Intermittent fasting boosts antitumor immunity by restricting CD11b^(+)Ly6C^(low)Ly6G^(low) cell viability through glucose metabolism in murine breast tumor model

Intermittent fasting can benefit breast cancer patients undergoing chemotherapy or immunotherapy.However,it is still uncertain how to select immunotherapy drugs to combine with intermittent fasting.Herein we observed that two cycles of fasting treatment significantly inhibited breast tumor growth and lung tissue metastasis,as well as prolonged overall survival in mice bearing 4T1 and 4T07 breast cancer.During this process,both the immunosuppressive monocytic-(M-)and granulocytic-(G-)myeloid-derived suppressor cell(MDSC)decreased,accompanied by an increase in interleukin(IL)7R^(+)and granzyme B^(+)T cells in the tumor microenvironment.Interestingly,we observed that Ly6G^(low)G-MDSC sharply decreased after fasting treatment,and the cell surface markers and protein mass spectrometry data showed potential therapeutic targets.Mechanistic investigation revealed that glucose metabolism restriction suppressed the splenic granulocytemonocyte progenitor and the generation of colony-stimulating factors and IL-6,which both contributed to the accumulation of G-MDSC.On the other hand,glucose metabolism restriction can directly induce the apoptosis of Ly6G^(low)G-MDSC,but not Ly6G^(high)subsets.In summary,these results suggest that glucose metabolism restriction induced by fasting treatment attenuates the immune-suppressive milieu and enhances the activation of CD3^(+)T cells,providing potential solutions for enhancing immune-based cancer interventions.

Bakuchiol ameliorates glycolipid homeostasis by reducing inflammation

Inflammatory stimulation plays a significant role in the development and worsening of insulin-resistant diabetes.Therefore,it is crucial to identify therapeutic agents that can alleviate insulin resistance by targeting inflammation.Here,we present evidence that Bakuchiol(BL),a monoterpene phenolic compound first discovered from Psoralea corylifolia L.as traditional Chinese medicine,can effectively improve insulin resistance in diabetic mice through anti-inflammation.Our findings demonstrate that BL alleviates inflammation by inhibiting the toll-like receptor 4/nuclear factorκB/mitogen-activated protein kinase axis,consequently enhancing insulin receptor signaling through the c-Jun N-terminal kinase/suppressors of cytokine signaling 3/insulin receptor substrate1 pathway and improving glucolipid homeostasis.Furthermore,the insulin recovery achieved with BL(60 mg/kg)was comparable to that of metformin(200 mg/kg).These results provide further support for considering BL as a potential treatment option for insulin-resistant diabetes mellitus.

Structural characteristics,anticoagulant and antithrombotic mechanism of a novel polysaccharide from Rosa Chinensis Flos

This is the first report on a polysaccharide(RCJ2-Ib)isolated from Rosa Chinensis Flos.RCJ2-Ib was obtained through the extraction with water,precipitation with ethanol,separation with DEAE-52 column and purification with DEAE-Sepharose Fast Flow column and Sephadex G100 column.GC,FT-IR and NMR analyses revealed that RCJ2-Ib(3.3 k Da)was a 1,4-linked polymannuronic acid containing substantialβ-Danomers units.The anticoagulant effect of RCJ2-Ib evaluated by using rabbit ear venous blood and an acute blood stasis rat model showed that RCJ2-Ib had obvious anticoagulant activity in regulating endogenous and exogenous coagulation pathways and reducing serum thromboxane B2 and endothelin-1.In addition,RCJ2-Ib could also increase the number of Lactobacillus and Escherichia coli.As a result,RCJ2-Ib has the potential to inhibit thrombosis and maintain the intestinal environment.

Immunomodulatory effects of polysaccharides from edible fungus:a review

The polysaccharides from edible fungus showed many kinds of biological activities,including anti-tumor,immunomodulatory,anti-inflammatory,anti-diabetes,improving functional constipation activities.In particular,the immunomodulatory effects have been paid more and more attention by scholars,but there was no systematic introduction of their immunomodulatory mechanism.So,this review introduced the immunomodulatory effects and mechanism of edible fungus polysaccharides in recent years,and then the relationships between structure and immunomodulatory effect were also discussed.

Comprehensive Evaluation of New Self-cultivated Sugarcane Lines by Grey Relational Analysis

The grey relational analysis( GRA) was used to evaluate the 10 sugarcane lines by 13 main agronomic characters. The result showed that the grey correlation degree of self-cultivated lines GNY08- 320(0. 7502),GNY08- 336(0. 7409),GNY08- 186(0. 7369),and GNY08- 225(0. 7277) were higher than ROC22(CK),so the 4 lines can be further tested,and the others were worse than CK that should be further observed or eliminated.

国产精子计数板在长白公猪精液检测上的应用研究

文章以精子计数板为研究对象,应用SPSS 19.0软件的单因素方差分析法对81份精液检测数据进行分析,以探讨国产的精子计数板代替进口的精子计数板的可行性。试验结果显示,进口产品组精子计数板测得的随机3个视野平均精子数、精子密度、精子活力、正常精子比例分别为566.06个、249.56×10^6/ml、92.65%、71.52%;国产产品组的对应值测得为577.02个、252.68×10^6/ml、91.32%、72.65%。两产品的同一指标相比均差异不显著。结果表明:国产的精子计数板替代进口的精子计数板是可行的。

Polyhydric Corrole and Its Gallium Complex： Synthesis, DNA-binding Properties and Photodynamic Activities

A new hydrophilic polyhydric corrole （1） and its Ga（III） complex （1-Ga） were synthesized and characterized. Corroles 1 and 1-Ga bind to calf thymus DNA externally and exhibit remarkable photonuclease-like activity. Singlet oxygen （102） was identified as the reactive oxygen species involved. These corroles also exhibit significant photocytotoxicity against HeLa, A549, BEL-7402 and HepG2 tumor cell lines. They mainly accumulated in the nuclei of tumor cells. ROS-mediated mitochondrial damage and intracellular DNA disintegration contributed to the observed photodynamic activity.