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穗花杉双黄酮对3T3-L1细胞增殖、凋亡及生物钟的影响 被引量:7
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作者 王曜晖 赵智权 +2 位作者 杜运松 袁冰舒 王刚 《中国现代医学杂志》 CAS 2019年第16期1-9,共9页
目的探讨穗花杉双黄酮对3T3-L1细胞增殖、凋亡及生物钟的影响。方法采用不同浓度穗花杉双黄酮处理正常和过表达昼夜节律运动输出周期故障蛋白(CLOCK)的3T3-L1细胞,CCK-8和EdU检测细胞增殖情况;流式细胞仪检测细胞凋亡情况;real-timeRT-... 目的探讨穗花杉双黄酮对3T3-L1细胞增殖、凋亡及生物钟的影响。方法采用不同浓度穗花杉双黄酮处理正常和过表达昼夜节律运动输出周期故障蛋白(CLOCK)的3T3-L1细胞,CCK-8和EdU检测细胞增殖情况;流式细胞仪检测细胞凋亡情况;real-timeRT-PCR检测生物钟基因CLOCK和BMAL1的表达;Westernblotting检测Cleavedcaspase-3、Bcl-2、CLOCK及BMAL1蛋白的表达。结果低浓度(2.5~10.0mg/L)穗花杉双黄酮作用于3T3-L1细胞后,3T3-L1细胞增殖和凋亡无影响(P>0.05),但是当浓度达20和40mg/L反而抑制增殖(P<0.05);通过上调Cleavedcaspase-3表达,下调Bcl-2表达,促进凋亡的发生。较低浓度穗花杉双黄酮对3T3-L1细胞CLOCK、BMAL1基因和蛋白的表达无影响(P>0.05),而浓度达20和40mg/L则呈抑制作用(P<0.05);过表达CLOCK基因可减弱穗花杉双黄酮对3T3-L1细胞的抑制作用(P<0.05)。结论3T3-L1细胞在培养状态仍保持昼夜节律性,较高浓度穗花杉双黄酮可抑制3T3-L1细胞增殖并促进凋亡,其可作为抑制细胞增殖的药物,其作用机制与生物钟基因表达有关。 展开更多
关键词 双黄酮类 细胞增殖 细胞凋亡 生物钟
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Protective Effect of Chlorogenic Acid against Carbon Tetrachloride-induced Acute Liver Damage in Rats 被引量:4
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作者 Zong-xi Sun Song Liu +1 位作者 zhi-quan zhao Rui-qiang Su 《Chinese Herbal Medicines》 CAS 2014年第1期36-41,共6页
Objective To evaluate the protective effect of chlorogenic acid (CGA) on carbon tetrachloride (CCh)-induced liver injury of rats. Methods The anti-oxidative activity of CGA was investigated with several establishe... Objective To evaluate the protective effect of chlorogenic acid (CGA) on carbon tetrachloride (CCh)-induced liver injury of rats. Methods The anti-oxidative activity of CGA was investigated with several established in vitro systems. The hepatoprotective activity of CGA against CCI4-induced acute liver injury in eats was studied. The levels of alanine aminotranferase (ALT), aspartate aminotransferase (AST), alkaline phosphatase (ALP), and total bilirubin (TB) were measured. The histopathological examination was carried out to supplement the biochemical results. Results CGA possessed strong anti-oxidative ability in vitro. The CCh-induced liver toxicity experiment showed that the rats pretreated with CGA (300 or 500 mg/kg) had lower levels of ALT, AST, ALP, and TB than those of the CCI4-treated group. These data were supplemented with histopathological examination of rat liver sections. CGA did not show any mortality at the dose up to 5000 mg/kg. Conclusion CGAcould protect the liver againstCCI4-induced oxidative damage in rats, and the possible mechanism of the activity may be due to its free radical-scavenging and anti-oxidative activity. 展开更多
关键词 acute liver damage ANTI-OXIDATION carbon tetrachloride chlorogenic acid HEPATOPROTECTION
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