Integrating Geology and Engineering for Viscous Oil Production Improvement—JX Oilfield, Bohai Bay, China

In this paper a case study is presented where refined 3D reservoir geology models, well pattern pilot test and Real-time GeoSteering tools have been integrated to optimize production performance of a viscous oil reserve. The viscous reserves were of high structural dip angle. In addition delta depositional system represented highly variable geomorphology, where stacked sandbodies and shale bedding are crossing each other frequently. In order to keep a higher production rate, using horizontal wells along with water injection was not enough;therefore, detailed reservoir characterization, well pattern pilot experiment and GeoSteering were used to optimize previous development strategy and keep horizontal trajectories safely landing into reservoir target zone. The stratigraphic sequence architecture that is derived from seismic interpretations captured the variation within these high dip structural backgrounds very effectively. The best combination of choices was “Injecting Water outside from OWC” and “Stair Shaped Horizontal Trajectories”. The borehole collision risks of these optimized strategies were then analyzed and controlled successfully by the GeoSteering tools during trajectory landing process. The reservoir development performance is improved tremendously as result of these renewed development strategies.

Study on Water Injection Formula by Grey Correlation Method for Offshore Water Flooding Reservoir

Based on the development of the offshore water drive reservoir, the determination of reasonable water injection of monolayer and single well affects the distribution of remaining oil and development effect of oil field water flooding, so this paper, using the grey correlation analysis method, studies the main factors influencing the dividing coefficient of water-injection and weight. This method takes into account the effective thickness, permeability, injection-production well spacing, the number of injection-production wells, and the direction of formation coefficient factors such as the influence of the dividing coefficient for injection. The formula for water-injection multi-factor controlled is established, at the same time, applying water injection formula of this article and the conventional on injection allocation of A-platform offshore injection wells. And the difference of single-well injection-allocation quantity in layers is analyzed. Based on a platform offshore, layers exist in the process of water flooding and the problems such as the contradiction between the layers, put forward the necessity of layered water injection through this article to determine water injection formula and injection allocation on a platform of injection wells by implementing effect which can be found. A platform for water flooding development of water cut under control, natural decline rate reduced to verify the reliability and practicability of the formula to determine injection in this paper. It has certain guiding significance to the offshore for the water-injection development oilfield.

Impacts of different surface features on soil detachment in the subtropical region

Near-surface features have a great influence on runoff and detachment processes by overland flow,but the contributions are still unclear on steep slopes with yellow soil in subtropical humid regions.Field scouring experiments were conducted to investigate how near-surface features affect hydraulic parameters and detachment rate by overland flow.Five treatments and a baseline(disturbed rootless bare cropland)were designed to identify the contributions from stem-leaves,litter,biological soil crusts(BSCs),root systems and non-disturbance,respectively.The results showed that(1)the values of velocity,Reynolds number and Froude number for vegetated slopes were significantly lower than that of baseline,and stem-leaves made a greater contribution(average of 47.30%)to reducing kinetic energy than the other features;(2)the total contribution rate of grassland was 99.38%,and of this total,1.19,1.44,2.49,49.79 and 44.47%reductions were attributed to the stem-leaves,litter,BSCs,root systems and non-disturbance,respectively.Root system and non-disturbance dominated the detachment rate reduction;(3)with increasing flow rate or slope gradient,the total contribution rate remained between 98%and 100%;and(4)for each treatment,the relationship between detachment rate and hydraulic parameters remained constant,and the stream power was the best predictor to detachment rate.The study results are helpful in evaluating the effects of near-surface features on erosion control and providing reference for government decision-makers to choose appropriate soil conservation and management practices.

Integrative cytology and proteome alterations unravel the multitarget effect of a novel alkaloid antofine against Penicillium italicum

Penicillium italicum is the causal agent of citrus blue mold,which is a major threat to the global citrus fruit industry.Antofine,a natural phenanthroindolizidine alkaloid,is water-soluble and exhibits a broad range of biological activities.However,whether it can inhibit P italicum growth and the potential inhibitory mechanism remains to be elucidated.This study aimed to investigate the antifungal mechanism of antofine against P italicum using scanning electron microscopy,transmission electron microscopy(TEM),propidium iodide staining,and tandem mass tag-labeled quantitative proteomic analysis.Antofine was found to exhibit its preeminent antifungal activity against P italicum with a minimum inhibitory concentration of 1.56 mg/L and a minimum fungicidal concentration of 6.25 mg/L.The challenge test revealed that antofine inhibited the development of citrus blue mold during a 6-d P italicum-infected period.Antofine acted on its potential multitargets to inhibit P italicum growth by synergistically activating oxidative stress through accumulating excess reactive oxygen species,impairing membrane integrity.inducing membrane lipid peroxidation,and disrupting mitochondrial function,thereby disrupting the membrane system and reducing cell via-bility.Moreover,antofine treatment downregulated most differentially expressed proteins involved in carbon metabolism,pyruvate metabolism,and the tricarboxylic acid cycle(TCA)in P italicum mycelia,which may explain the mitochondrial decomposition observed by TEM and the de-clines in ATP levels as well as the activities of TCA-related enzymes.These results indicate that antofine treatment inhibited P italicum growth by targeting the cell membrane and mitochondria.