Development and Validation of an Automatic Ultrawide-Field Fundus Imaging Enhancement System for Facilitating Clinical Diagnosis:A Cross-Sectional Multicenter Study

In ophthalmology,the quality of fundus images is critical for accurate diagnosis,both in clinical practice and in artificial intelligence(AI)-assisted diagnostics.Despite the broad view provided by ultrawide-field(UWF)imaging,pseudocolor images may conceal critical lesions necessary for precise diagnosis.To address this,we introduce UWF-Net,a sophisticated image enhancement algorithm that takes disease characteristics into consideration.Using the Fudan University ultra-wide-field image(FDUWI)dataset,which includes 11294 Optos pseudocolor and 2415 Zeiss true-color UWF images,each of which is rigorously annotated,UWF-Net combines global style modeling with feature-level lesion enhancement.Pathological consistency loss is also applied to maintain fundus feature integrity,significantly improving image quality.Quantitative and qualitative evaluations demonstrated that UWF-Net outperforms existing methods such as contrast limited adaptive histogram equalization(CLAHE)and structure and illumination constrained generative adversarial network(StillGAN),delivering superior retinal image quality,higher quality scores,and preserved feature details after enhancement.In disease classification tasks,images enhanced by UWF-Net showed notable improvements when processed with existing classification systems over those enhanced by StillGAN,demonstrating a 4.62%increase in sensitivity(SEN)and a 3.97%increase in accuracy(ACC).In a multicenter clinical setting,UWF-Net-enhanced images were preferred by ophthalmologic technicians and doctors,and yielded a significant reduction in diagnostic time((13.17±8.40)s for UWF-Net enhanced images vs(19.54±12.40)s for original images)and an increase in diagnostic accuracy(87.71%for UWF-Net enhanced images vs 80.40%for original images).Our research verifies that UWF-Net markedly improves the quality of UWF imaging,facilitating better clinical outcomes and more reliable AI-assisted disease classification.The clinical integration of UWF-Net holds great promise for enhancing diagnostic processes and patient care in ophthalmology.

MicroRNA396-mediated alteration in plant development and salinity stress response in creeping bentgrass

The conserved microRNA396(miR396)is involved in plant growth,development,and abiotic stress response in multiple plant species through regulating its targets,Growth Regulating Factor(GRF)transcription factor genes.However,the role of miR396 has not yet been characterized in perennial monocot species.In addition,the molecular mechanism of miR396-mediated abiotic stress response remains unclear.To elucidate the role of miR396 in perennial monocot species,we generated transgenic creeping bentgrass(Agrostis stolonifera)overexpressing Osa-miR396c,a rice miRNA396 gene.Transgenic plants exhibited altered development,including less shoot and root biomass,shorter internodes,smaller leaf area,fewer leaf veins,and epidermis cells per unit area than those of WT controls.In addition,transgenics showed enhanced salt tolerance associated with improved water retention,increased chlorophyll content,cell membrane integrity,and Na^(+)exclusion during high salinity exposure.Four potential targets of miR396 were identified in creeping bentgrass and up-regulated in response to salt stress.RNA-seq analysis indicates that miR396-mediated salt stress tolerance requires the coordination of stress-related functional proteins(antioxidant enzymes and Na+/H+antiporter)and regulatory proteins(transcription factors and protein kinases).This study establishes a miR396-associated molecular pathway to connect the upstream regulatory and downstream functional elements,and provides insight into the miRNA-mediated regulatory networks.

MiR396 is involved in plant response to vernalization and flower development in Agrostis stolonifera

MicroRNA396(miR396)has been demonstrated to regulate flower development by targeting growth-regulating factors(GRFs)in annual species.However,its role in perennial grasses and its potential involvement in flowering time control remain unexplored.Here we report that overexpression of miR396 in a perennial species,creeping bentgrass(Agrostis stolonifera L.),alters flower development.Most significantly,transgenic(TG)plants bypass the vernalization requirement for flowering.Gene expression analysis reveals that miR396 is induced by long-day(LD)photoperiod and vernalization.Further study identifies VRN1,VRN2,and VRN3 homologs whose expression patterns in wild-type(WT)plants are similar to those observed in wheat and barley during transition from short-day(SD)to LD,and SD to cold conditions.However,compared to WT controls,TG plants overexpressing miR396 exhibit significantly enhanced VRN1 and VRN3 expression,but repressed VRN2 expression under SD to LD conditions without vernalization,which might be associated with modified expression of methyltransferase genes.Collectively,our results unveil a potentially novel mechanism by which miR396 suppresses the vernalization requirement for flowering which might be related to the epigenetic regulation of VRN genes and provide important new insight into critical roles of a miRNA in regulating vernalization-mediated transition from vegetative to reproductive growth in monocots.

Alterations and Its Mechanisms of Wnt Signal Pathway in Human High-matastatatic Large Cell Lung Cancer Cell Line L9981 by Transfecting with Nm23-H1 Gene

Backgroud and Objective Tumor metastasis is not only the malignant marker and characteristics of lung cancer, but also the main cause of failure to cure and lose their life of the

Effects of Different Picking Time and Different Geographical Provenances on Oil Content of Camellia oleifera

This paper studied effects of different picking time and different geographical provenances on oil content of Camellia oleifera. The results showed that different picking time had significant effects on the oil content of cold dew seeds and frost's descent seeds. With the delay of picking time,the oil content of cold dew seeds,frost's descent seeds,dry seeds and oil content of seed kernels were significantly increased.There was a significantly positive correlation between the oil content of fresh fruit of cold dew seeds and frost's descent seeds and the dry seed yield of fresh seeds and the oil content of fresh fruit( P < 0. 05),and the correlation coefficient was greater than 0. 85. Besides,the oil content of seed kernels of cold dew seeds was closely correlated with the seed yield of fresh seeds,oil content of fresh fruit,and oil content of fresh seeds. In the process of C. oleifera breeding,the thin coat,high seed yield and high oil content can be taken as the key research directions for future breeding. The oil content of ordinary C. oleifera seeds in 18 counties( cities) was 36. 42%-63. 33%,indicating that there were significant differences in the oil content of C. oleifera in different geographical provenances. In conclusion,according to the study of different picking time,the recommended picking time of cold dew seeds in Hunan area is around October 10,while the best picking time of frost's descent seeds is about October 30. The oil content of C. oleifera fruit in different geographical provenances is quite different. During the development of C. oleifera fruit,the cultivation and management of C. oleifera should be strengthened to increase the oil content of C. oleifera.

Construction of Rooting System during Camellia oleifera Tissue Culture

An rooting experiment of tissue culture plantlets was carried out with sterile plantlets obtained from the stem segments of a good clone of Camellia oleifera as materials. The results showed that basic medium and illumination condition are factors crucial to rooting of C. oleifera. With 1/4MS as basic medium, the treat- ment with the addition of 0.5 mg/L NAA and soaking in 2 000 mg/L KIBA, containing 30 mg/L sucrose and subjected to dark culture of 20 d was the optimal treat- ment, achieving a rooting rate of 86. 7%.

Electro-acupuncture therapy to improve spatial learning and memory in APPswe/ PS1dE9 transgenic mice through the inhibition of the TLR4/MyD88 signaling pathway

Objective:To determine whether electro-acupuncture (EA) therapy could improve the cognitive functions of amyloid precursor protein Swedish mutation (APPswe)/presenilin 1 deleted in exon 9 (PS1dE9) mice and examine whether EA treatment could attenuate neuroinflammation by targeting the toll-like receptor 4 (TLR4)/myeloid differentiation primary response factor 88 (MyD88) signaling pathway.Methods:Twenty-seven double transgenic APPswe/PS1dE9 mice were randomly allocated into three groups:an Alzheimer's disease model group (AD group),a medication group (M group) and an EA treatment group (EA group).Each group contained nine mice,and nine wild-type mice were used in a normal group (N group).The animals in the M group were treated with oral administrations of 0.92 mg/kg donepezil hydrochloride for 15 days.For animals in the EA group,EA treatments were used on the Yintang (GV 29) and Baihui (GV 20) acupoints for 20 minutes,and the Shuigou (GV 26) acupoint was pricked without needle retention following EA treatments.Following treatments,the spatial learning and memory of the mice were measured using the Morris water maze test.The expression levels of TLR4,MyD88,nuclear factor kappa B (NF-κB) and inducible nitric oxide synthase (iNOS) were analyzed by immunohistochemical staining and western blot.Results:The escape latencies of the M and EA groups were significantly lower than those of the AD group (vs M,P =.002;vs EA,P <.001).Moreover,compared with the AD group,the numbers of platform crossings was higher (vs M,P =.038;vs EA,P =.008) and the latency time for target quadrants was longer (vs M,P =.002;vs EA,P =.001) in the M and EA groups (P <.05).Furthermore,in the M and EA groups,the expression levels of TLR4,MyD88,NF-κB and iNOS decreased significantly compared with those of the AD group (all P <.01).Conclusion:EA treatment enhanced the memory and learning abilities of APPswe/PS1dE9 mice by regulating the TLR4/MyD88 inflammatory signaling pathway.

Research on Carrier Synchronization of QPSK Based on Simulink

As an important part of the communication system, synchronization technology is a communication system to run the correct protection, has been a hot topic in communication systems research. Four-phase phase shift keying (QPSK) circuit structure is relatively simple, and has a good band utilization, in the modern communication has a wide range of applications. Corresponding research on carrier synchronization for QPSK coherent demodulation is carried out. By comparing several carrier recovery loops, The quaternary loose tail loop is selected as carrier recovery loop. First of all, the core part of the digital phase-locked loop analysis and implementation, and finally through the tool Simulink to complete the realization of the system.

Changes in Physiological and Biochemical Characteristics of Floral Organ Development in a Soybean Cytoplasmic-nuclear Male Sterile Line

[Objectives]This study was conducted to explore the mechanism of soybean cytoplasmic-nuclear male sterility.[Methods]With soybean cytoplasmic-nuclear male sterile line JLCMS9 A and its homotype maintainer line JLCMS9 B as experimental materials,the activity of superoxide dismutase(SOD),peroxidase(POD)and catalase(CAT),malondialdehyde(MDA)content,starch content,soluble protein content,soluble sugar content and free proline content in flower buds,alabastrums and mature flowers were determined,and the contents and changes of auxin(IAA),gibberellin(GA3),isopentenyl adenosine(iPA)and abscisic acid(ABA)at the three stages were analyzed.[Results]The activity of SOD and CAT and the contents of MDA and free proline in the sterile line at the flower bud stage were lower than those of the maintainer line,but the opposite was true at the alabastrum stage and the flowering stage,and their values were higher than those of the maintainer line;the POD activity of the sterile line was significantly lower than that of the maintainer line at the flower bud stage,and the opposite was true at the alabastrum stage and the flowering stage,and its values were higher than those of the maintainer line;and the starch content and soluble sugar content of sterile line 9 A showed an overall upward trend,and were significantly lower than those of the maintainer line 9 B at the alabastrum stage and the flowering stage.During the whole development process of floral organs,the content of IAA in sterile line 9 A showed a trend of first increasing and then decreasing,and the content of iPA increased gradually.The contents of hormones in the sterile line were lower than those in the maintainer line.The ratios of IAA/ABA,IAA/GA3,IAA/iPA and ABA/GA3 in the sterile line were significantly different from those in the maintainer line.It is inferred that the abnormal physiological characteristics of floral organ development are related to the cytoplasmic-nuclear male sterility of soybean.The alabastrum stage may be a critical period for the occurrence of abnormal physiological and biochemical indexes in the floral organs of soybean cytoplasmic-nuclear male sterile lines.[Conclusions]This study provides a theoretical basis for the breeding of fine sterile lines of soybean and the research on the mechanism of sterility.

The Inhibiting Effects and It's Molecular Mecanisms of the Fungi of Huai Er's on Human Large Cell Lung Cancer Cell Line L9981

Background and Objective Lung cancer, which threatens human’s health and life, is the malignant tumor with the most rapid increase of morbidity. Although recent years the basic

Down-regulation and It's Molecular Biology of Nm-23H1 Gene Transfection on PKC Signal Path-way in Human High-metastatic Large Cell Lung Cancer Cell Line L9981

Backgroud and Objective At present, lung cancer has become the most frenquent malignant tumor in China and in the world which its mortality and morbidity is increasing fastest.

Changes of the Proteomic Profiles in Human High-metastatic Large Cell Lung Cancer Cell Line L9981 by Transfecting with nm23-H1 Gene

Background and Objective Cancer metastasis is not only the malignant characteristics of lung cancer, but also the key cause of failure to cure and high mortality. It has been proved

The Regulating Mechanism of Nm23-H1 Gene for FAK Signal Pathway in Human Lung Cancer Cell Line L9981

Background and objective Lung Cancer is one of the most malignant cancers threatening people’s health and life and one of the most rapid increasing cancers both in morbidity

Down-regulation and It's Molecular Biology of Nm-23H1 gene Transfection on Ras-to-MAPK Signal Transduction Pathway in Human High-metastatic Large Cell Lung Cancer Cell Line L9981

Backgroud and Objective Tumor metastasis is not only the malignant marker and characteristics of lung cancer, but also the key cause of failure to cure and lose their life of the patients

Leaf Photosynthetic Characteristics of High-Yield Soybean[Glycine max(L.)Merr.]Under Different Planting Densities,Different Fertilization Rates,and Single/Mixed Planting Patterns

Understanding the photosynthetic characteristics of high-yield soybean[Glycine max(L.)Merr]cultivar(HYC)would aid research aiming at investigating the soybean high yield formation mechanism and optimization of cultivation system.To assess the photosynthesis of HYC,a pot experiment was conducted to quantify the differences in photosynthetic characteristics between HYC and common-yield soybean cultivar(CC)under different planting densities,fertilization rates,and single/mixed planting patterns.The leaf greenness(Lg),net photosynthetic rate(Ph),stomatal conductance(St)and transpiration rate(Tr)were significantly higher in HYC than CC mainly in seed-filling stages.HYC was more tolerant to dense and mixed planting because the decreases of Ph and St under high planting density and those of Ph,St,and Tr under mixed planting were lower in HYC than CC.The Lg and Ph in HYC were more superior to those in CC at high fertilization rate.Thus,the HYC has a superior performance in photosynthetic characteristics under the varied cultivation practices,which may contribute to the greater seed yield in HYC than CC.

Fast Acquisition Method of GPS Signal Based on FFT Cyclic Correlation

Since the global positioning system began to operate, it has become more and more close to people’s lives, and has been applied to various fields now. In order to track and decode GPS signals, GPS signals need to be captured first. The necessary parameters of the captured GPS signal are immediately transmitted to the tracking process, and then the navigation message of the satellite can be obtained by tracking process. In this paper, the basic contents related to the signal structure of GPS system are briefly described. Then, the traditional GPS signal acquisition method based on time domain correlation method is introduced, and the GPS signal acquisition method based on FFT cyclic correlation method is discussed in this paper. By comparing the simulation results, two kinds of GPS signal acquisition methods are compared with the calculation time according to the method of controlling variables. For the two GPS signal acquisition methods, the variation of time delay error with SNR is simulated in this paper.

A Comparative Assessment of On-Site Application Effects of Mine Dust Prevention and Control Technology

Prolonged exposure to coal dust leads to various lung disorders, including incurable coal workers’ pneumoconiosis (CWP), and endangers miners’ health in underground mines. This article summarizes the latest research progress in dust control technology, including chemical dust suppressants, foam dust removal, ultrasonic atomization, magnetized water dust suppression, double curtains of wind and fog, biological nano-film, and emerging microbial dust suppressants in the field of dust. The actual application compares and analyzes the advantages and disadvantages of different dust removal technologies. The current three directions of mine dust prevention and control are pointed out: the prevention and control of respirable dust, hydrophobic dust, and secondary dust, and the prospects for future development trends. Given the treatment of respirable dust, it is necessary to strengthen the research on the dust generation mechanism under different working conditions and to explore the migration and settlement laws of respirable dust by constructing a numerical model of dust dynamics;for the treatment of hydrophobic dust, further research on the microphysical and chemical properties of coal dust is needed. The relationship between wettability and continuously optimizing the wetting agent;for the prevention and control of secondary dust in coal mines, emerging microbial dust suppression technologies need to be developed to explore the micro-action between microorganisms and coal dust molecules to achieve green prevention and control of dust.

Effects of acupuncture on serotonin,histamine,substance P,and tryptase levels at sensitized points in model rats with knee osteoarthritis

Objective:To elucidate the differences in manual acupuncture effectiveness at sensitized points by investigating the mechanisms of local skin action at different sensitization points in rats with knee osteoarthritis(KOA).Methods:Forty SpragueeDawley rats were equally divided into control,model(1 mg of mono-iodoacetate into the right knee joint cavity),sham operation,manual acupuncture at right Tianjing acupoint(MAR-SJ 10),and left SJ 10 groups.Safranine-O and fast green staining were used to assess the modeling.The morphological and functional changes in mast cells(MCs)were assessed during acupoint sensitization using toluidine blue and immunofluorescence staining.The levels of serotonin,histamine,substance P(SP),and tryptase at skin acupoints and serum levels of IL-β,IL-6,and TNF-αwere detected using ELISA.Results:After 14 days of treatment,the number of MCs and their degranulation rates were statistically higher in the model group than in the control group(both P<0.001).After applying acupuncture,the levels of 5-HT,HA,and SP at skin acupoints were lower than those in the model group(all P<0.05),and tryptase level was higher(both P<0.05).Tryptase level was higher on the skin at the MAL-SJ 10 acupoint than that on the MAR-SJ 10 acupoint(P=0.004).Compared with the model group,the serum levels of IL-1β,IL-6,and TNF-αin the MAR-SJ 10 and MAL-SJ 10 groups were lower(all P<0.05).Conclusion:Acupuncture at KOA-sensitized acupoints mitigates joint injury in KOA rats and may bidi-rectionally regulate local MCs of these acupoints.This finding not only enhances the reference value of sensitizing points in clinical diagnosis and treatment,but also contributes to the understanding of the biological mechanisms underlying acupuncture intervention at sensitizing points.

Reduced graphene oxide aerogel decorated with Mo_(2)C nanoparticles toward multifunctional properties of hydrophobicity,thermal insulation and microwave absorption

Reduced graphene oxide(rGO)aerogels are emerging as very attractive scaffolds for high-performance electromagnetic wave absorption materials(EWAMs)due to their intrinsic conductive networks and intricate interior microstructure,as well as good compatibility with other electromagnetic(EM)components.Herein,we realized the decoration of rGO aerogel with Mo_(2)C nanoparticles by sequential hydrothermal assembly,freeze-drying,and high-temperature pyrolysis.Results show that Mo_(2)C nanoparticle loading can be easily controlled by the ammonium molybdate to glucose molar ratio.The hydrophobicity and thermal insulation of the rGO aerogel are effectively improved upon the introduction of Mo_(2)C nanoparticles,and more importantly,these nanoparticles regulate the EM properties of the rGO aerogel to a large extent.Although more Mo_(2)C nanoparticles may decrease the overall attenuation ability of the rGO aerogel,they bring much better impedance matching.At a molar ratio of 1:1,a desirable balance between attenuation ability and impedance matching is observed.In this context,the Mo_(2)C/r GO aerogel displays strong reflection loss and broad response bandwidth,even with a small applied thickness(1.7 mm)and low filler loading(9.0wt%).The positive effects of Mo_(2)C nanoparticles on multifunctional properties may render Mo_(2)C/r GO aerogels promising candidates for high-performance EWAMs under harsh conditions.

Acid Soil Is Associated with Reduced Yield, Root Growth and Nutrient Uptake in Black Pepper (<i>Piper nigrum</i>L.)

Low pH is a major limiting factor for the production of black pepper (Piper nigrum L.) in Hainan province. Black pepper gardens often exhibit a decrease in soil pH (to 5.5 - 5.0) on orchards with a multi-year production history. An exploratory hydroponic experiment was conducted to examine the effects of increasingly acid nutrient solution pH (7.0, 5.5, 4.0, and 3.5) on seedling growth, tissue nutrient concentrations and root morphological traits. The results indicated that low pH may directly inhibit root development and function, limit K, Ca and Mg absorption and reduce seedling growth. At pH 5.5, black pepper attained maximum growth, while the minimum growth occurred at pH 3.5. It can be concluded that low pH reduces plant growth and is associated with low root nutrient concentrations of Ca and Mg, which may explain the decline of the yield in the seven pepper gardens of the Institute.