Transcriptomics-based identification and characterization of genes related to sugar metabolism in‘Hongshuijing’pitaya

Sugar composition not only affects fruit flavor but is also an important determinant of fruit taste and consumer preference.In this study,changes in the sugar content and sugar-metabolizing enzymes were investigated from different sections of various fruit development phases of‘Hongshuijing’pitaya(Hylocereus monacanthus).Genes related to sugar metabolism were also screened by transcriptome analyses.The results indicated that glucose was the major sugar in mature pitaya fruit,and was mainly regulated by vacuolar acid invertase(VAI)and sucrose synthase(SS)(degradative direction).Sugar accumulation varied in pulp between different sections of the pitaya fruit.VAI,neutral invertase(NI)and SS(degradative direction)are crucial enzymes for sugar accumulation in pitaya.The expression of 17 genes related to sucrose metabolism obtained from seven databases[NCBI non-redundant protein database(Nr),NCBI non-redundant nucleotide sequence database(Nt),EuKaryotic Orthologous Groups(KOG),The Protein Families(Pfam),Kyoto Encyclopedia of Genes and Genomes(KEGG),Swiss-prot,and Gene Ontology(GO)]were analyzed in different pitaya pulp sections.HpVAI1 had the highest relative expression level on the 29th day after pollination(DAP).Positive correlations were found between HpVAI1 expression and VAI activity;HpNI4 and NI activity;HpSS2,HpSS5,and SS activity(synthetic direction),indicating that HpVAI1,HpNI4,and HpSS2 and HpSS5 were involved in the regulation of VAI,NI,and SS(synthetic direction),respectively.HpVAI1 and HpNI4 regulated sucrose degradation and the accumulation of glucose and fructose,while HpSS2 and HpSS5 regulated sucrose synthesis.These results suggest that HpVAI1 plays a key role in sugar metabolism during fruit development of‘Hongshuijing’pitaya.The results of this study provide new information about sugar metabolism in pitaya fruit that could help improve fruit quality and the breeding of new cultivars.

Removal of the main inflorescence to induce reflowering of loquat

Loquat(Eriobotrya japonica Lindl.)is an evergreen fruit tree species of the Rosaceae,and its unique flowering time greatly hinders its production.To explore the artificial regulation of loquat flowering time,we removed the main inflorescence(by cutting it)to induce reflowering.For different loquat tree cultivars with different stages,the inflorescence was removed by cutting the main floral axis at two alternative positions:the upper or the lower position beneath the inflorescence,and it was found that the proportion of reflowering resulting from removing the upper position of the main floral axis of yellow-flesh loquat cultivars during the full-bloom stage was the highest.In addition,compared with those of the normal-growing panicles,the number of flower buds and branch axes of the reflowering panicles decreased significantly after cutting.Importantly,these newly produced inflorescences flowered 2–4 months later than normal-growing inflorescences did,effectively prolonging both the flowering and fruiting time.In addition,qRT-PCR results showed that EjFT1,EjFT2,EjAP1–1 and EjAP1–2 were highly expressed in the floral axis.These findings highlighted a new method for extending the production cycle of loquat and provided a reference for the flowering regulation of loquat and other economically important fruit tree species.

Eight-channel microwave photonics transceiver photonic integrated circuit

Microwave photonics(MWP)studies the interaction between microwaves and light waves,including the generation,transmission,and processing of microwave signals.Integrated MWP using photonic integrated circuits(PICs)can achieve compact,reliable,and green implementation.However,most PICs have recently been developed that only contain one or a few devices.Here,we propose a multi-channel PIC that covers almost all devices in MWP.Our PIC integrates lasers,modulators,amplifiers,and detectors in the module,successfully manufacturing an eight-channel array transceiver module.We conducted performance tests on the encapsulated transceiver module and found that the cascaded bandwidth of the eightchannel transceiver module was greater than 40 GHz,and the spurious-free dynamic range(SFDR)of the broadband array receiver module was greater than 94 dBm·Hz2/3.The noise figure(NF)is less than-35 dB and the link gain is greater than-26 dB.The success of multi-channel PIC marks a crucial step forward in the implementation of large-scale MWP.

Optical coherent dot-product chip for sophisticated deep learning regression

Optical implementations of neural networks(ONNs)herald the next-generation high-speed and energy-efficient deep learning computing by harnessing the technical advantages of large bandwidth and high parallelism of optics.However,due to the problems of the incomplete numerical domain,limited hardware scale,or inadequate numerical accuracy,the majority of existing ONNs were studied for basic classification tasks.Given that regression is a fundamental form of deep learning and accounts for a large part of current artificial intelligence applications,it is necessary to master deep learning regression for further development and deployment of ONNs.Here,we demonstrate a silicon-based optical coherent dot-product chip(OCDC)capable of completing deep learning regression tasks.The OCDC adopts optical fields to carry out operations in the complete real-value domain instead of in only the positive domain.Via reusing,a single chip conducts matrix multiplications and convolutions in neural networks of any complexity.Also,hardware deviations are compensated via in-situ backpropagation control provided the simplicity of chip architecture.Therefore,the OCDC meets the requirements for sophisticated regression tasks and we successfully demonstrate a representative neural network,the AUTOMAP(a cutting-edge neural network model for image reconstruction).The quality of reconstructed images by the OCDC and a 32-bit digital computer is comparable.To the best of our knowledge,there is no precedent of performing such state-of-the-art regression tasks on ONN chips.It is anticipated that the OCDC can promote the novel accomplishment of ONNs in modern AI applications including autonomous driving,natural language processing,and scientific study.

Ultra-compact four-lane hybrid-integrated ROSA based on three-dimensional microwave circuit design

An ultra-compact hybrid-integration receiver optical subassembly(ROSA) with four channels is demonstrated in our laboratory with the size of 23.3 mm × 6.0 mm × 6.5 mm. The ROSA is comprised of a planar lightwave circuit(PLC) arrayed waveguide grating(AWG) chip, a top-illuminated positive-intrinsic-negative photodetector array chip, and a three-dimensional microwave circuit that is specially designed for compact packaging. For each transmission lane, the -3 dB bandwidth of the ROSA is up to 20 GHz, and the maximum responsivity is up to 0.53 A/W. The proposed package structure can be used for smaller package sizes and would be an easy assembling solution for 100 GbE optical communication devices.

112 Gbit/s transmitter optical subassembly based on hybrid integrated directly modulated lasers

Based on the hybrid integration technology, an ultra-compact and low cost transmitter optical subassembly module is proposed. Four directly modulated lasers are combined with a coarse wavelength division multiplexer operated at the O-band. The bandwidth for all channels is measured to be approximately 3 GHz. The 112 Gb∕s transmission is experimentally demonstrated for a 10 km standard single mode fiber（SSMF）, in which an optical isolator is used for avoiding the back-reflected and scattered light to improve the bit error rate（BER） performance. A low BER and clear eye opening are achieved for 10 km transmission.

Package-level passive equalization technology enabling DML-based 112 Gbps/λ PAM4 transmission

We demonstrate a package-level passive equalization technology in which the wire-bonding-induced resonance effect is used to compensate for the limited gain strength within the Nyquist frequency.The corresponding gain strength under various inductance and capacitance combinations could be quantitatively determined using a numerical simulation.With the increase in the Nyquist frequency,the capacitance shows a greater effect on the gain strength than the inductance.Therefore,the parasitic capacitance should be decreased to realize the desired gain strength at a higher Nyquist frequency.With this equalization technology,gain strength of 5.8 dB is obtained at 22 GHz,which can compensate for the limited bandwidth for the 112 Gbps pulse amplitude modulation(PAM4)signal.The experimental results show that 112 Gbps/λ PAM4 transmission based on a directly modulated laser(DML)module can be realized with a bit error rate of 1×10^-3 at a received optical power of 3d Bm.