Saccharides are a sort of ubiquitous and vital molecules within the whole life.However,the application of saccharides analysis with matrix-assisted laser desorption/ionization mass spectrometry(MALDI-MS)is restricted ...Saccharides are a sort of ubiquitous and vital molecules within the whole life.However,the application of saccharides analysis with matrix-assisted laser desorption/ionization mass spectrometry(MALDI-MS)is restricted by their low ionization efficiency and the instability of the sialic acid fraction.Derivatization strategy based on nonreductive amination provides a good solution,however,this is often time consuming and may result in sample loss due to removal of excessive derivatization reagents.Herein,hydralazine(HZN)was utilized as a reactive matrix for labeling reducing saccharides directly on MALDI target which eliminated tedious sample preparation and avoided sample loss.After optimization,effective and reproducible on-MALDI-target derivatization of neutral and acidic saccharides was achieved in both positive and negative modes.Compared with 2,5-dihydroxybenzoic acid(DHB)and 9-aminoacridine(9-AA),HZN improved the detection sensitivity of reducing saccharides and provided more abundant fragment ions in MS/MS analysis.Moreover,26 kinds of neutral glycans and 5 kinds of sialic glycans were identified from ovalbumin(OVA)and bovine fetuin,respectively.Combined with the statistical models,this strategy could be used to distinguish and predict samples of 6 brands of beer,and discriminate 2 kinds of beer fermentation modes.In addition,HZN was applied for quantitative analysis of glucose in urine samples,and the obtained urine glucose concentrations of diabetic patients were consistent with the clinical test results,showing the potential of qualitative and quantitative analysis of reducing saccharides in complex samples.展开更多
Amyloid beta-peptide 1-42(Aβ1-42)is one of the biomarkers of Alzheimer's disease,and its selective capture and quantitative detection are important for diagnosis and treatment of Alzheimer's disease.Herein,co...Amyloid beta-peptide 1-42(Aβ1-42)is one of the biomarkers of Alzheimer's disease,and its selective capture and quantitative detection are important for diagnosis and treatment of Alzheimer's disease.Herein,copper(Ⅱ)ions-immobilized virus-like hollow covalent organic frameworks(V-HCOFs@Cu^(2+))were synthesized by a facile approach.The as-prepared V-HCOFs@Cu^(2+)showed unique morphology,ultra-high specific surface(2552 m^(2)/g),uniform mesoporous structure(3.2 nm),superior chemical stability and abundant binding sites.Based on these excellent properties,the V-HCOFs@Cu^(2+)could be adopted as an ideal enrichment probe for highly efficient capture of Aβ1-42,exhibiting high adsorption capacity(320 mg/g),and fast adsorption equilibration time(3 min).In addition,an attractive approach of the V-HCOFs@Cu^(2+)-based matrix-assisted laser desorption/ionization mass spectrometry(MALDI-MS)was developed for the rapid screening and quantitative analysis of Aβ1-42 in human serum by using C-peptide as an internal standard,which exhibited low limit of detection(LOD,0.2 fmol/μL),and satisfactory recovery.This work provides an alternative solution for enrichment of biomarkers and also offers the potential applications of COFs in clinical analysis.展开更多
基金supported by a grant from the National Natural Science Foundation of China(Nos.22274021,21974021 and 22036001)the Natural Science Foundation of Fujian Province(No.2022J01535)the Major Project of Science and Technology of Fujian Province(No.2020HZ06006)。
文摘Saccharides are a sort of ubiquitous and vital molecules within the whole life.However,the application of saccharides analysis with matrix-assisted laser desorption/ionization mass spectrometry(MALDI-MS)is restricted by their low ionization efficiency and the instability of the sialic acid fraction.Derivatization strategy based on nonreductive amination provides a good solution,however,this is often time consuming and may result in sample loss due to removal of excessive derivatization reagents.Herein,hydralazine(HZN)was utilized as a reactive matrix for labeling reducing saccharides directly on MALDI target which eliminated tedious sample preparation and avoided sample loss.After optimization,effective and reproducible on-MALDI-target derivatization of neutral and acidic saccharides was achieved in both positive and negative modes.Compared with 2,5-dihydroxybenzoic acid(DHB)and 9-aminoacridine(9-AA),HZN improved the detection sensitivity of reducing saccharides and provided more abundant fragment ions in MS/MS analysis.Moreover,26 kinds of neutral glycans and 5 kinds of sialic glycans were identified from ovalbumin(OVA)and bovine fetuin,respectively.Combined with the statistical models,this strategy could be used to distinguish and predict samples of 6 brands of beer,and discriminate 2 kinds of beer fermentation modes.In addition,HZN was applied for quantitative analysis of glucose in urine samples,and the obtained urine glucose concentrations of diabetic patients were consistent with the clinical test results,showing the potential of qualitative and quantitative analysis of reducing saccharides in complex samples.
基金supported by a grant from the National Natural Science Foundation of China(Nos.21974021,22036001,and 91843301).
文摘Amyloid beta-peptide 1-42(Aβ1-42)is one of the biomarkers of Alzheimer's disease,and its selective capture and quantitative detection are important for diagnosis and treatment of Alzheimer's disease.Herein,copper(Ⅱ)ions-immobilized virus-like hollow covalent organic frameworks(V-HCOFs@Cu^(2+))were synthesized by a facile approach.The as-prepared V-HCOFs@Cu^(2+)showed unique morphology,ultra-high specific surface(2552 m^(2)/g),uniform mesoporous structure(3.2 nm),superior chemical stability and abundant binding sites.Based on these excellent properties,the V-HCOFs@Cu^(2+)could be adopted as an ideal enrichment probe for highly efficient capture of Aβ1-42,exhibiting high adsorption capacity(320 mg/g),and fast adsorption equilibration time(3 min).In addition,an attractive approach of the V-HCOFs@Cu^(2+)-based matrix-assisted laser desorption/ionization mass spectrometry(MALDI-MS)was developed for the rapid screening and quantitative analysis of Aβ1-42 in human serum by using C-peptide as an internal standard,which exhibited low limit of detection(LOD,0.2 fmol/μL),and satisfactory recovery.This work provides an alternative solution for enrichment of biomarkers and also offers the potential applications of COFs in clinical analysis.
