Seismic Response of Adjacent Structure to Inter-Story Isolated Structure

Currently, land resources are becoming more and more constrained and structures are getting closer to each other. To investigate the seismic response of inter-story isolated structure to adjacent structure, models considering no soil-structure interaction (SSI), considering soil-structure interaction (SSI), and considering structure-soil-structure interaction (SSSI) were established. Nonlinear seismic response comparative analysis was conducted by varying the spacing between adjacent structure and inter-story isolated structure, as well as the weight of adjacent structure, under different earthquake inputs, in order to obtain the structural response characteristics. The results indicate that the inter-story drift and inter-story shear of the inter-story isolated structure without considering SSI are smaller than those considering SSI and SSSI. The inter-story drift and inter-story shear of the inter-story isolated structure considering SSSI are further affected compared to that of the inter-story isolated structure considering only SSI. As the spacing between adjacent structure and inter-story isolated structure increases, the influence of adjacent structure on inter-story isolated structure decreases. The variation in the spacing between the two structures has a negligible effect on the isolation layer of the inter-story isolated structure. With the increase in the weight of adjacent structure, the influence of adjacent structure on inter-story isolated structure becomes more significant. The increasing weight of adjacent structure has an increasing effect on the Isolation layer of the inter-story isolated structure.

Electroacupuncture at Du channel and meridian of foot-Taiyang for hippocampal neurons in rats with depression

BACKGROUND： Long-term anti-depression treatment can promote the regeneration of hippocampal regeneration. Up-regulation of hippocampal regeneration can reverse or prevent against the injury of stress to cerebrum, especially to hippocampal structure and function. Therefore, promoting hippocampal neuronal regeneration may be a new strategy for treating depression and anxiety, OBJECTIVE： To observe the effect of electro-acupuncture at Du channel and meridian of foot-Taiyang on hippocampal neurons from model rats of depression. DESIGN： A randomized controlled animal experiment. SETTING： Department of Acupuncture and Moxibustion, Shenzhen Affiliated Hospital, Guangzhou University of Traditional Chinese Medicine. MATERIALS： Twenty-four Wistar rats, of either gender, aged 2 months old, weighing 200 - 220 g, were provided by the Animal Experimental Center, Guangzhou University of Traditional Chinese Medicine. METHODS： This experiment was carried out in the Clinical Molecular Biochemical Laboratory, Shenzhen Affiliated Hospital, Guangzhou University of Traditional Chinese Medicine between October 2006 and April 2007. ①The involved rats were randomized into 4 groups according to body mass： blank control group, model group, electroacupuncture A group and electroacupuncture B group, with 6 in each. Rats in the blank control group were free to access to water, and were not given any intervention. Rats in the latter 3 groups were developed into rat depression models by chronic stress combined with feeding alone, and received 21-day unpredictable various stresses. Rats in the model group were euthanized at 14 days after modeling, and their brain tissues were harvested. Rats in the electroacupuncture A group were modeled, then points ＂Baihui＂ and ＂Shenting＂ were chosen, and given electroacupuncture, once a day, 20 minutes once. Rats in the electroacupuncture B group were modeled, then points ＂Baihui＂, ＂Shenting＂, ＂Xinshu＂and ＂Ganshu＂ were chosen, and frequency and therapeutic time were the same as those of electroacupuncture A group. Rats in the latter 2 groups were also given electroacupuncture, and their brain tissues were harvested 14 days later. ②Index evaluation： Neurons in hippocampal CA 1 and CA3 regions as well as dentate gyrus [neuron specific enolase （NSE）-positive cells] were counted by immunofluorescent technique. Each rat was weighted on the day of grouping and before execution at 35 days, MAIN OUTCOME MEASURES： Neuron amount in each hippocampal region and body mass of rats in each group. RESULTS： Twenty-four rats were involved in the final analysis.①Neuronal amount in each region of hippocampus： Neurons in the hippocampal CA1 and CA3 regions as well as dentate gyrus of rats in the model group were significantly less than those in the blank control group （P 〈 0.01）. Neurons in hippocampal CAland CA3 regions as well as dentate gyrus of rats in the electroacupuncture A and B groups were more than those in the model group （P 〈 0.05）. ② Change in body mass of rats： Body mass increase of rats in the model group was less than that in the blank control group （P 〈 0.05）, and body mass increase of electroacupuncture A and B groups was significantly more than that in the model group, respectively （P 〈 0.01）. CONCLUSION： Electroacupuncture at Du channel and meridian of foot-Taiyang alleviates hippocampal neuronal injury of rats, and thereby, improves depression and promotes the increase of body mass of rats.

Electro-acupuncture at Conception and Governor vessels and transplantation of umbilical cord bloodderived mesenchymal stem cells for treating cerebral ischemia/reperfusion injury

Mesenchymal stem cell transplantation is a novel means of treating cerebral ischemia/reper- fusion, and can promote angiogenesis and neurological functional recovery. Acupuncture at Conception and Governor vessels also has positive effects as a treatment for cerebral ischemia/ reperfusion. Therefore, we hypothesized that electro-acupuncture at Conception and Governor vessels plus mesenchymal stem cell transplantation may have better therapeutic effects on the promotion of angiogenesis and recovery of neurological function than either treatment alone. In the present study, human umbilical cord blood-derived mesenchymal stem cells were isolated, cultured, identified and intracranially transplanted into the striatum and subcortex of rats at 24 hours following cerebral ischemia/reperfusion. Subsequently, rats were electro-acupunctured at Conception and Governor vessels at 24 hours after transplantation. Modified neurological severity scores and immunohistochemistry findings revealed that the combined interventions of electro-acupuncture and mesenchymal stem cell transplantation clearly improved neurological impairment and up-regulated vascular endothelial growth factor expression around the isch- emic focus. The combined intervention provided a better outcome than mesenchymal stem cell transplantation alone. These findings demonstrate that electro-acupuncture at Conception and Governor vessels and mesenchymal stem cell transplantation have synergetic effects on promot- ing neurological function recovery and angiogenesis in rats after cerebral ischemia/reperfusion.

Influence of Ren and Du meridian electro-acupuncture on neural stem cell proliferation and extracellular signal-regulated kinase pathway in a rat model of focal cerebral ischemia injury

BACKGROUND： Studies have shown that electro-acupuncture at the Ren meridian could improve proliferation of subventricular zone neural stem cells in cerebral-ischemic rats. However, there are few reports on the influence of electro-acupuncture at the Du meridian on neural stem cell proliferation. OBJECTIVE： To observe the influence of electro-acupuncture at Ren and Du meridians on neural stem cell proliferation in the subventricular zone and altered signal transduction in cerebral ischemia rats. DESIGN, TIME AND SETTING： A randomized, controlled, animal experiment was performed at the Laboratory of Human Anatomy, Medical College of Sun Yat-sen University from May 2006 to February 2008. MATERIALS： Mouse anti-rat bromodeoxyuridine （BrdU） monoclonal antibody was provided by Sigma, USA; mouse anti-rat nestin monoclonal antibody and extracellular signal-regulated protein kinase （ERK） specific inhibitor PD98059 were provided by Calbiochem, Germany; acupuncture needle was provided by Suzhou Acupuncture Supplies, China. METHODS： A total of 126 rats were randomly assigned to four groups： model （n = 36）, Du meridian （n = 36）, Ren/Du meridian （n = 36）, and Ren/Du meridian ＋ PD98059 （n = 18）. Rats in the Ren/Du meridian ＋ PD98059 group were observed on days 7 （n = 6） and 14 （n = 12） after cerebral ischemia injury. Rats in the model, Du meridian, and Ren/Du meridian groups were observed on days 7, 14, and 28 after cerebral ischemia injury, with 12 rats per group at each time point. Thread occlusion was used to establish middle cerebral artery occlusion models. Electro-acupuncture was performed at Renzhong （DU 26） and Baihui （DU 20） acupoints in the Du meridian group, as well as Chengjiang （RN 24）, Guanyuan （RN 4）, Renzhong, and Baihuiacupoints in the Ren/Du meridian and Ren/Du meridian ＋ PD98059 groups 2 days after model establishment. In addition, electro-acupuncture stimulation with disperse-dense waves was performed, with 30 Hz disperse wave, 100 Hz dense wave, and 5 V intensity for 20 minutes. Rats in the Ren/Du meridian ＋ PD98059 group were treated with 0.2 pg PD98059 injection into the subventricular zone, 2 pL per rat. Rats in the model group were not treated with electro-acupuncture. MAIN OUTCOME MEASURES： BrdU/nestin immunofluorescent staining was used to detect proliferating neural stem cells in the subventricular zone of cerebral ischemia rats; Western blot was used to determine phosphorylated ERK1 and 2 （pERK1/2） expression in the subventricular zone. RESULTS： On days 14 and 28 after cerebral ischemia, there were significantly more BrdU-positive and BrdU/nestin-positive cells in the Ren/Du meridian group compared with the Du meridian group （P 〈 0.05）. PD98059 decreased the number of BrdU-positive and BrdU/nestin-positive cells induced by electro-acupuncture at the/：ten and Du meridians （P 〈 0.05）. On days 7, 14, and 28 after treatment, pERK1/2 expression was significantly greater in the Du meridian and Ren/Du meridian groups compared with the model group （P 〈 0.05）. The promoting effect of electro-acupuncture at Ren and Du meridians on ERK1/2 phosphorylation was superior to electro-acupuncture at the Du meridian alone on day 14 after model induction （P 〈 0.05）. However, PD98059 completely abolished the promoting effect of electro-acupuncture at Ren/Du meridians on pERK1/2 expression （P 〈 0.05）. CONCLUSION： Electro-acupuncture at Ren and Du meridians increased proliferation of subventricular zone neural stem cells, which was related to activation of the ERK pathway in a rat model of cerebral ischemia injury.

Effect of electro-acupuncture on basic fibroblast growth factor protein and mRNA expression in hippocampal dentate gyrus of spleen deficiency rats

BACKGROUND： Spleen deficiency in traditional Chinese medicine refers to the functional disorder of spleen, pancreas, intestines, and nervous system in modern medicine. OBJECTIVE; To test whether electro-acupuncture could alter basic fibroblast growth factor （bFGF） protein and mRNA expression in the hippocampal dentate gyrus of spleen deficiency rats. DESIGN, TIME AND SETTING： The randomized, controlled, in vivo animal experiment was performed at the National LeveI-B Laboratory of Clinical Cell Molecule and Biology in Shenzhen Hospital of Traditional Chinese Medicine, between March and November in 2008. MATERIALS： Reserpine injection was produced by Guangdong Bangmin Pharmaceutical Co. Rhubarb extract granule preparation was produced by Guangdong Yifang Pharmaceutical. Huanqiu Brand sterile acupuncture pin was provided by Suzhou Acupuncture Supplies, China. Huatuo Brand electroacupuncture instrument （type SDZ-II） was purchased from Suzhou Medical Appliance Factory, China. METHODS： A total of 96 male Sprague Dawley rats were randomly assigned to control （n = 32） and induction （n = 64） groups. Spleen deficiency was induced via intraperitoneal injection of reserpine and intragastric administration of rhubarb. The successful models were randomized into two groups： model and electro-acupuncture, with 32 rats in each group. Electro-acupuncture was administered at Zusanfi （ST 36） and Sanyinjiao （SP 6） acupoints using a condensation wave and rarefaction （condensation wave 15 Hz） at a strength of 6-15 V for 20 minutes, once per day. The appearance of a slight shiver in the corresponding locus was taken as the standard. According to electro- acupuncture time points, each group was assigned to four subgroups at 7, 14, 28, and 49 days, respectively, with eight rats in each subgroup. Immunohistochemical staining, image analysis, and reverse-transcription polymerase chain reaction were performed at different time points. MAIN OUTCOME MEASURES： bFGF protein and mRNA expression in the hippocampal dentate gyrus of spleen deficiency rats. RESULTS： After 7 days of electro-acupuncture therapy, bFGF protein and mRNA expression significantly increased compared with the model and control groups （P 〈 0.05）. After 14 days, bFGF protein and mRNA expression decreased until 28 days, where levels were then equal to the model group and greater than the control group （P 〈 0.05）. After 49 days, the above indices remained increased in the electro-acupuncture group compared to the model and control groups （P 〈 0.05）. CONCLUSION： Continuous electro-acupuncture maintained a high level of bFGF protein and mRNA expression in the hippocampal dentate gyrus of spleen deficiency rats.

Application of S-transform threshold filtering in Anhui experiment airgun sounding data de-noising

As a relatively new method of processing non-stationary signal with high time-frequency resolution, S transform can be used to analyze the time-frequency characteristics of seismic signals. It has the following characteristics: its time-frequency resolution corresponding to the signal frequency, reversible inverse transform, basic wavelet that does not have to meet the permit conditions. We combined the threshold method, proposed the S-transform threshold filtering on the basis of S transform timefrequency filtering, and processed airgun seismic records from temporary stations in "Yangtze Program"(the Anhui experiment). Compared with the results of the bandpass filtering, the S transform threshold filtering can improve the signal to noise ratio(SNR) of seismic waves and provide effective help for first arrival pickup and accurate travel time. The first arrival wave seismic phase can be traced farther continuously, and the Pm seismic phase in the subsequent zone is also highlighted.

Time-dependent effects of electroacupuncture at the Ren channel on extracellular signal-regulated kinases 1/2 expression in focal cerebral ischemia rats

BACKGROUND： The onset of focal cerebral ischemia activates extracellular signal-regulated kinases 1 and 2, regulates cell cycle, promotes cell proliferation and differentiation, and affects the normal stage and function of brain cells. OBJECTIVE： To observe the effects of electroacupuncture at the Ren channel on extracellular signal-regulated kinases 1/2 expression in the lateral cerebral ventricle wall of rats with focal cerebral ischemia. The effects were analyzed at different time points after intervention. DESIGN： Randomized controlled study. SETTING： Department of Anatomy, Sun Yat-Sen University. MATERIALS： A total of 60 healthy adult male Wistar rats weighing （250±10） g were provided by the Experimental Animal Center, Medical College of Sun Yat-Sen University. The animal experiment was conducted with confirmed consent by the local ethics committee. The GB6805-Ⅱ electric acupuncture apparatus was provided by Shanghai Medical Equipment High-techno Company. METHODS： The experiment was performed at the Laboratory of Anatomy, Sun Yat-Sen University, from February to July 2007. All experimental animals were randomly divided into the following groups： normal group （n = 6）, sham operation group （n = 18）, model group （n = 18）, and electroacupuncture group （n = 18）. Middle cerebral artery occlusion （MCAO） was performed in the model group and electroacupuncture group. Zea Longa＇s grading standard was used to assess neurological impairment after reperfusion; animals whose grades were between l and 4 were included in this study. The normal control group was not exposed to MCAO. In sham operation animals, the right common carotid artery （CCA） was isolated, and the external carotid artery （ECA） was damaged, but no embolism was induced. The electroacupuncture group was given acupuncture on the second day after surgery. The acupoint locations were chosen according to Experimental Acupuncture （People＇s Publishing House; 1997; First Edition）. The Chengjiang, Qihai, and Guanyuan acupoints were labeled and connected to a G6805 electroacupuncture apparatus with sparse-dense waves （sparse waves were 30 Hz, dense waves were 100 Hz）, with a frequency of 6-15 V. The duration was 20 minutes. Two days after surgery, the model and sham operation groups were placed with their backs on the operating table, but they received no acupuncture. However, the normal group received acupuncture. The experimental animals under anesthesia were sacrificed on days 7, 14, and 28 post-surgery. Western blot analysis was used to measure expression of extracellular signal-regulated kinases 1/2 in the inferior region of the lateral cerebral ventricle wall. Expression was measured in the normal group at time points corresponding to the sham operation group. MAIN OUTCOME MEASURES： Expression of extracellular signal-regulated kinases 1/2 in the inferior region of the lateral cerebral ventricle wall at different time points after intervention. RESULTS： All 60 rats were included in the final analysis, without any loss. Seven days after MCAO, there was no significant difference in extracellular signal-regulated kinases 1/2 expression in the electroacupuncture group compared to the model group （P 〉 0.05）. However, extracellular signal-regulated kinases 1/2 expression significantly increased in the model group at 14 and 28 days after treatment （P 〈 0.05）. CONCLUSION： Electroacupuncture at the Ren channel can enhance extracellular signal-regulated kinasesl/2 expression in the inferior region of the lateral cerebral ventricle wall of rats with focal cerebral ischemia. However, this effect is not apparent until 14 days after electroacupuncture intervention.

S-wave velocity structure in Tangshan earthquake region and its adjacent areas from joint inversion of receiver functions and surface wave dispersion

Using the seismic records of 83 temporary and 17 permanent broadband seismic stations deployed in Tangshan earthquake region and its adjacent areas(39°N–41.5°N,115.5°E–119.5°E),we conducted a nonlinear joint inversion of receiver functions and surface wave dispersion.We obtained some detailed information about the Tangshan earthquake region and its adjacent areas,including sedimentary thickness,Moho depth,and crustal and upper mantle S-wave velocity.Meanwhile,we also obtained the vP/vS structure along two sections across the Tangshan region.The results show that:(1)the Moho depth ranges from 30 km to 38 km,and it becomes shallower from Yanshan uplift area to North China basin;(2)the thickness of sedimentary layer ranges from 0 km to 3 km,and it thickens from Yanshan uplift region to North China basin;(3)the S-wave velocity structure shows that the velocity distribution of the upper crust has obvious correlation with the surface geological structure,while the velocity characteristics of the middle and lower crust are opposite to that of the upper crust.Compared with the upper crust,the heterogeneity of the middle and lower crust is more obvious;(4)the discontinuity of Moho on the two sides of Tangshan fault suggests that Tangshan fault cut the whole crust,and the low vS and high vP/vS beneath the Tangshan earthquake region may reflect the invasion of mantle thermal material through Tangshan fault.

Electroacupuncture at Ren channel point combined with injection of basic fibroblast growth factors for neural stem cell proliferation in subventricular zone of cerebral ischemic side

BACKGROUND： Electroacupuncture at Ren channel point can treat stroke and generate some neurodevelopment-related growth factors, which are associated with the proliferation and differentiation of stem cells. OBJECTIVE： To observe the effect of electroacupuncture at Ren channel point combined with injection of basic fibroblast growth factors （bFGF） on the expression of 5-bromo-2-deoxyuridine （BrdU）-Iabeled and BrdU/Neuroepthelial stem cell protein （Nestin）-Iabeled positive cells in subventricular zone of cerebral ischemic rats. DESIGN ： Completely randomized grouping design and controlled trial.SETTING： Department of Acupuncture and Moxibustion, Shenzhen Hospital, Guangzhou University of Traditional Chinese Medicine. MATERIALS ： Eighty-four adult healthy male Wistar rats were involved and divided into 5 groups by a lot： blank group （n =6）, sham-operation group （n =6）, model group （n =26）, injection of bFGF group （bFGF group, n =24） and electroacupuncture at Ren channel combined with injection of bFGF group （bFGF group, n =22）. Three time points were set in each of the latter three groups for observation： 7, 14 and 28 days after ischemia respectively. Mouse BrdU and Nestin monoclonal antibody （analytical pure, China）, G6805 electro-acupuncture apparatus （Huayi Medical Instrument, Shanghai） and Olympus FV 500 laser confocal microscope （Olympus Company） were used. METHODS ： This trial was carried out in the Laboratory of Anatomy, Medical College of Sun Yat-sen University from September 2004 to July 2005. ① Except for blank group and sham-operation group, the rats in the other groups were prepared into rat models of local cerebral ischemia by suture-occluded method. The rats were reperfused, and then they were graded in neurologic deficits based on neurological scoring of Longa. Rat model was successful when the neurological scoring ranged from 1 to 4 points and used for the trial. Blank group was untouched; Right common carotid artery was isolated and right external carotid artery was dissected without embolism in the sham-operation group. On the 2nd day after operation, the rats in the combination group were given acupuncture at Chengjiang, Qihai and Guanyuan point, then, electro-acupuncture apparatus, with sparse wave 30 Hz, dense wave 100 Hz, intensity 6 to 15 V, was used to stimulate these three points. Stimulation standard was based on the slight trill appeared in the corresponding position, and the stimulation lasted 20 minutes. After being acupunctured, the rats were intramuscularly injected with 4 000 U/d, and administration was immediately after reperfusion, after this, bFGF was intramuscularly injected following acupuncture, once a day. In the bFGF group, the rats were intramuscularly injected with bFGF on the 2^nd day after operation. The dosage and time of injection were the same as those in the combination group. The rats in the model group and sham-operation group were mounted on the acupuncture bench for 20 minutes on the 2^nd day after operation, and acupuncture was omitted. ② The expressions of BrdU-labeled positive cells （ reflect cell proliferation） and BrdU/Nestin-labeled positive cells （reflect neural stem cells proliferation） in the lateral subventricular zone were determined by immunofluorescence. Positive cells were counted in five visual fields using 200-fold Olympus FV 500 laser confocal microscope system. ③ One-way analysis of variance was used for multiple comparison of the mean of samples. MAIN OUTCOME MEASURES ： The effect of electroacupuncture combined with injection of bFGF on the number of BrdU and BrdU/Nestin-labeled positive cells in subventricular region of cerebral ischemic side. RESULTS： Altogether 8,6 and 4 rats in model group, combination group and bFGF group died respectively during modeling, finally 6 rats respectively in the normal group and sham-operation group and 18 rats respectively Le., 6 rats at each time point, in the model group, combination group and bFGF group were involved in the result analysis.① 7 days after cerebral ischemia, BrdU and BrdU/Nestin-labeled positive cells in the model group were （272.6±36.9）and （65.3±11.8）/5 visual fields, respectively, which were more than those of normal group [（47.2±6.1） ,（15.6±4.2）/5 visual fields, P 〈 0.01], but less than those in the bFGF group [（365.5±42.7 ）, （82.9±12.2）/5 visual fields] and in the combination group [（427.8±46.9） ,（125.6±18.5）/5 visual fields, P 〈 0.01]. The BrdU and BrdU/Nestin-labeled positive cells in the bFGF group were significantly less than those in the combination group （P 〈 0.05）. ② 14 days after cerebral ischemia, the BrdU and BrdU/Nestin-labeled positive cells in the model group were significantly more than those in the blank group [（184.7±28.1 ）,（46.5±9.6）/5 visual fields, P 〈 0.01], but less than those in the bFGF group [（259.4＋32.8）,（68.1 ± 10.1 ）/5 visual fields] and combination group [（308.6±34.2）,（87.2±16.9）/5 visual fields, P 〈 0.01]. The BrdU and BrdU/Nestin-labeled positive cells in the bFGF group were significantly less than those in the combination group （P〈0.05）. ③ 28. days after cerebral ischemia, the BrdU and BrdU/Nestin-labeled positive cells in the model group were close to those in the normal group [（51.3±8.4）,（17.8±5.3）/5 visual fields, P 〉 0.05], but significantly less than those in the bFGF group [（128.1 ±23.6）,（41.3±11.4）/5 visual fields]and in the combination group [（162.5±26.1）,（62.3±15.8）/5 visual fields, P〈 0.01]. The BrdU and BrdU/Nestin-labeled positive cells in the bFGF group were significantly less than those in the combination group （P〈 0.05）. ④ There were no significant differences in BrdU/Nestin-labeled and Nestin-labeled positive cells between sham-operation group and normal group（P 〉 0.05）. CONCLUSION ： Electroacupuncture at Ren channel point alone or combined with injection of bFGF can significantly promote neural stem cell proliferation of rats after local cerebral ischemia. Electroacupuncture at Ren channel point and injection of bFGF have synergistic effect.

Combinatorial effects of conception and governor vessel electroacu-puncture and human umbilical cord blood-derived mesenchymal stem cells on pathomorphologic lesion and cellular apoptosis in rats with cerebral ischemia/reperfusion

OBJECTIVE: To investigate the combinatorial effects of conception and governor vessel electroacupuncture(EA) and human umbilical cord blood-derived mesenchymal stem cells(HUCB-MSCs) on pathomorphologic lesion and cellular apoptosis in rats with cerebral ischemia/reperfusion. METHODS: With the HUCB-MSCs isolated, cultured and identified and the models of cerebral ischemia-reperfusion established, the HUCB-MSCs of passage three were intracranially transplanted and the EA at conception and governor vessels was applied. The pathomorphologic lesion by hematoxylin-eosin staining and the cellular apoptosis by terminal deoxynucleotidyl transferase-mediated nick-end labeling method around the ischemic focus were observed. RESULTS: The cultured adherent HUCB-MSCs exhibited a spindle shape and expressed MSC-specific markers, with the cell purity and proliferation rate significantly increasing after the primary passage. HE staining showed that there were no pathological changes observed in the sham surgery group. However, in the PBS transplantation group, degeneration and necrosis of a great number of nerve cells were seen. In both the HUCB-MSCs transplantation group and the HUCB-MSCs transplantation + EA group, reparative changes of the pathomorphism of the tissue were found. Both combination treatment and simple MSCs treatment were able to improve the pathomorphorlogic lesion following cerebral ischemia and reduce the abnormal TUNEL-positive numbers, with former better than latter. CONCLUSION: HUCB-MSCs improve pathological lesions and inhibit the cellular apoptosis around the cerebral ischemic area. EA at conception and governor vessels also improve pathological lesion and inhibit the cellular apoptosis in rats treated with HUCB-MSCs transplantation, which effects were superior to that of simple HUCB-MSCs transplantation.