Most mammalian cells take up cholesterol from low-density lipoproteins(LDLs) via receptor-mediated endocytosis.After reaching lysosomes,LDL-derived cholesterol continues to transport to downstream organelles including...Most mammalian cells take up cholesterol from low-density lipoproteins(LDLs) via receptor-mediated endocytosis.After reaching lysosomes,LDL-derived cholesterol continues to transport to downstream organelles including the ER for specific structural and functional needs.Peroxisomes are recently found to receive cholesterol from lysosomes through lysosomeperoxisome membrane contacts.However,whether and how cholesterol is conveyed from peroxisomes to the ER remain unknown.Here,by combining high-resolution microscopic analyses and in vitro reconstitution of highly purified organelles or artificial liposomes,we demonstrate that peroxisomes form membrane contacts with the ER through the interaction between peroxisomal PI(4,5)P2 and ER-resident extended synaptotagmin-1,2 and 3(E-Syts).Depletion of peroxisomal PI(4,5)P2 or ESyts markedly decreases peroxisome-ER membrane contacts and induces cholesterol accumulation in lysosomes.Furthermore,we show that cholesterol is delivered from 3H-labeled peroxisomes or PI(4,5)P2-containing liposomes to the ER in vitro,and that the presence of peroxisomes augments cholesterol transfer from lysosomes to the ER.Together,our study reveals a new cholesterol transport pathway along the lysosome-peroxisome-ER membrane contacts in the cell.展开更多
Can WiFi signals be used for sensing purpose？ The growing PHY layer capabilities of WiFi has made it possible to reuse WiFi signals for both communication and sensing. Sensing via WiFi would enable remote sensing wit...Can WiFi signals be used for sensing purpose？ The growing PHY layer capabilities of WiFi has made it possible to reuse WiFi signals for both communication and sensing. Sensing via WiFi would enable remote sensing without wearable sensors, simultaneous perception and data transmission without extra communication infrastructure, and contactless sensing in privacy-preserving mode. Due to the popularity of WiFi devices and the ubiquitous deployment of WiFi networks, WiFi-based sensing networks, if fully connected, would potentially rank as one of the world＇s largest wireless sensor networks. Yet the concept of wireless and sensorless sensing is not the simple combination of WiFi and radar. It seeks breakthroughs from dedicated radar systems, and aims to balance between low cost and high accuracy, to meet the rising demand for pervasive environment perception in everyday life. Despite increasing research interest, wireless sensing is still in its infancy. Through introductions on basic principles and working prototypes, we review the feasibilities and limitations of wireless, sensorless, and contactless sensing via WiFi. We envision this article as a brief primer on wireless sensing for interested readers to explore this open and largely unexplored field and create next-generation wireless and mobile computing applications.展开更多
基金supported by the National Natural Science Foundation of China (91754102, 31771568, 31690102, 31600651, 31701030)National Key Research and Development Project of the Ministry of Science and Technology of China (2016YFA0500100)+2 种基金Shenzhen City Technology Basic Research Program (JCYJ20170818144026198)Science and Technology Department of Hubei Province (2017CFB617)the 111 Project of Ministry of Education of China (B16036)
文摘Most mammalian cells take up cholesterol from low-density lipoproteins(LDLs) via receptor-mediated endocytosis.After reaching lysosomes,LDL-derived cholesterol continues to transport to downstream organelles including the ER for specific structural and functional needs.Peroxisomes are recently found to receive cholesterol from lysosomes through lysosomeperoxisome membrane contacts.However,whether and how cholesterol is conveyed from peroxisomes to the ER remain unknown.Here,by combining high-resolution microscopic analyses and in vitro reconstitution of highly purified organelles or artificial liposomes,we demonstrate that peroxisomes form membrane contacts with the ER through the interaction between peroxisomal PI(4,5)P2 and ER-resident extended synaptotagmin-1,2 and 3(E-Syts).Depletion of peroxisomal PI(4,5)P2 or ESyts markedly decreases peroxisome-ER membrane contacts and induces cholesterol accumulation in lysosomes.Furthermore,we show that cholesterol is delivered from 3H-labeled peroxisomes or PI(4,5)P2-containing liposomes to the ER in vitro,and that the presence of peroxisomes augments cholesterol transfer from lysosomes to the ER.Together,our study reveals a new cholesterol transport pathway along the lysosome-peroxisome-ER membrane contacts in the cell.
文摘Can WiFi signals be used for sensing purpose？ The growing PHY layer capabilities of WiFi has made it possible to reuse WiFi signals for both communication and sensing. Sensing via WiFi would enable remote sensing without wearable sensors, simultaneous perception and data transmission without extra communication infrastructure, and contactless sensing in privacy-preserving mode. Due to the popularity of WiFi devices and the ubiquitous deployment of WiFi networks, WiFi-based sensing networks, if fully connected, would potentially rank as one of the world＇s largest wireless sensor networks. Yet the concept of wireless and sensorless sensing is not the simple combination of WiFi and radar. It seeks breakthroughs from dedicated radar systems, and aims to balance between low cost and high accuracy, to meet the rising demand for pervasive environment perception in everyday life. Despite increasing research interest, wireless sensing is still in its infancy. Through introductions on basic principles and working prototypes, we review the feasibilities and limitations of wireless, sensorless, and contactless sensing via WiFi. We envision this article as a brief primer on wireless sensing for interested readers to explore this open and largely unexplored field and create next-generation wireless and mobile computing applications.
