Pseudoalteromonas sp. SM9913 is a phychrotmphic bacterium isolated from the deep-sea sediment. The genes encoding chaperones DnaJ and DnaK of P. sp. SM9913 were cloned by normal PCR and TAIL - PCR （GenBank accession ...Pseudoalteromonas sp. SM9913 is a phychrotmphic bacterium isolated from the deep-sea sediment. The genes encoding chaperones DnaJ and DnaK of P. sp. SM9913 were cloned by normal PCR and TAIL - PCR （GenBank accession Nos DQ640312, DQ504163 ）. The chaperones DnaJ and DnaK from the strain SM9913 contain such conserved domains as those of many other bacteria, and show some cold-adapted characteristics in their structures when compared with those from psychro-, meso-and themophilic bacteria. It is indicated that chaperones DnaJ and DnaK of P. sp. SM9913 may be adapted to low temperature in deep-sea and function well in assisting folding, assembling and translocation of proteins at low temperature. This research lays a foundation for the further study on the cold-adapted mechanism of chaperones DnaJ and DnaK of cold-adapted microorganisms.展开更多
The abundance and biomass of benthic heterotrophic bacteria were investigated for the 4 typical sampling stations in the northern muddy part of Jiaozhou Bay, estuary of the Dagu River, raft culturing and nearby areas ...The abundance and biomass of benthic heterotrophic bacteria were investigated for the 4 typical sampling stations in the northern muddy part of Jiaozhou Bay, estuary of the Dagu River, raft culturing and nearby areas of Huangdao in March, June, August and December, 2002. The abundance and biomass range from 0.98×107 to 16.87×107cells g-1 sediment and 0.45 to 7.08μgCg-1 sediment, respectively. Correlation analysis showed that heterotrophic bacterial abundance and biomass are significantly correlated to water temperature (R =0.79 and 0.83, respectively, P<0.01).展开更多
A protocol to coextract the microbial metagenomic DNA and RNA from deep-sea sediment was developed for the microbiological study of environmental samples. The obtained pure metagenomic DNA with the size larger than 23...A protocol to coextract the microbial metagenomic DNA and RNA from deep-sea sediment was developed for the microbiological study of environmental samples. The obtained pure metagenomic DNA with the size larger than 23 kb and stable RNA could be used directly for PCR and reverse transcription - PCR （ RT - PCR） respectively. The direct lysis including the treatments of SDS, proteinase and lysozyme was applied to acquiring the metagenomic DNA and RNA furthest. Prior to the lysis treatment, the glass bead and denaturing solution were added to enhance the lysis efficiency and keep the integrity of RNA respectively. Denaturing gradient gel electrophoresis （DGGE） was applied in accessing the microbial 16S rRNA diversity by PCR and RT -PCR amplification from a single extraction. The pattern obtained by this analysis revealed some differences between them, indicating the efficiency of the protocol in extracting the metagenomic DNA and total RNA from deep-sea sediment.展开更多
基金The work was supported by the Hi-Tech Research and Development Program of China under contract Nos 2006AA09Z414 and 2007AA091903；the China Ocean Mineral Resources R ＆ D Association under contract No. DYXM - 115 - 02 - 2 - 6；the National Natural Science Foundation of China under contract No. Z2004D02；the Natural Science Foundation of Shandong Province of China under contract No. Z2004D02；the Foundation for Young Excellent Scientists in Shandong Province of China under contract No. 2006BS02002；the Program for New Century Excellent Talents in University under contract No. NCET - 06 - 0578.
文摘Pseudoalteromonas sp. SM9913 is a phychrotmphic bacterium isolated from the deep-sea sediment. The genes encoding chaperones DnaJ and DnaK of P. sp. SM9913 were cloned by normal PCR and TAIL - PCR （GenBank accession Nos DQ640312, DQ504163 ）. The chaperones DnaJ and DnaK from the strain SM9913 contain such conserved domains as those of many other bacteria, and show some cold-adapted characteristics in their structures when compared with those from psychro-, meso-and themophilic bacteria. It is indicated that chaperones DnaJ and DnaK of P. sp. SM9913 may be adapted to low temperature in deep-sea and function well in assisting folding, assembling and translocation of proteins at low temperature. This research lays a foundation for the further study on the cold-adapted mechanism of chaperones DnaJ and DnaK of cold-adapted microorganisms.
基金funded by the National Key Basic Research Program from the Ministry of Science and Technology,the People's Republic of China(G1999043709,2002CTM12400)the National Natural science Foundation of China(Nos.40176033,40576061).
文摘The abundance and biomass of benthic heterotrophic bacteria were investigated for the 4 typical sampling stations in the northern muddy part of Jiaozhou Bay, estuary of the Dagu River, raft culturing and nearby areas of Huangdao in March, June, August and December, 2002. The abundance and biomass range from 0.98×107 to 16.87×107cells g-1 sediment and 0.45 to 7.08μgCg-1 sediment, respectively. Correlation analysis showed that heterotrophic bacterial abundance and biomass are significantly correlated to water temperature (R =0.79 and 0.83, respectively, P<0.01).
文摘A protocol to coextract the microbial metagenomic DNA and RNA from deep-sea sediment was developed for the microbiological study of environmental samples. The obtained pure metagenomic DNA with the size larger than 23 kb and stable RNA could be used directly for PCR and reverse transcription - PCR （ RT - PCR） respectively. The direct lysis including the treatments of SDS, proteinase and lysozyme was applied to acquiring the metagenomic DNA and RNA furthest. Prior to the lysis treatment, the glass bead and denaturing solution were added to enhance the lysis efficiency and keep the integrity of RNA respectively. Denaturing gradient gel electrophoresis （DGGE） was applied in accessing the microbial 16S rRNA diversity by PCR and RT -PCR amplification from a single extraction. The pattern obtained by this analysis revealed some differences between them, indicating the efficiency of the protocol in extracting the metagenomic DNA and total RNA from deep-sea sediment.
