目的:探讨罗伊氏乳杆菌对肠上皮细胞炎症反应和凋亡的作用及相关机制。方法:通过免疫荧光和酶联免疫吸附检测罗伊氏乳杆菌对肠上皮细胞炎症反应和凋亡的影响;采用生物信息学分析、定量聚合酶链式反应和双荧光素酶报告基因等实验筛选罗...目的:探讨罗伊氏乳杆菌对肠上皮细胞炎症反应和凋亡的作用及相关机制。方法:通过免疫荧光和酶联免疫吸附检测罗伊氏乳杆菌对肠上皮细胞炎症反应和凋亡的影响;采用生物信息学分析、定量聚合酶链式反应和双荧光素酶报告基因等实验筛选罗伊氏乳杆菌发挥作用的下游微小核糖核酸(microRNA,miRNA);通过双荧光素酶报告基因和Western blot等实验筛选miR-196a下游靶基因及其功能。结果:罗伊氏乳杆菌能够抑制脂多糖引起的肠上皮细胞炎症反应和凋亡,进一步分析发现罗伊氏乳杆菌通过上调肠上皮细胞miR-196a抑制细胞炎症和凋亡,miR-196a通过靶向抑制程序性细胞死亡因子4(programmed cell death 4,PDCD4)基因表达发挥对肠上皮细胞的保护作用。结论:罗伊氏乳杆菌通过miR-196a靶向抑制PDCD4基因表达,减缓肠上皮细胞炎症反应和凋亡。展开更多
A main requirement for cells to function normally is the availability of glucose.Glucose,available either direct from circulation or storage,is converted to the essential energy that cells need to drive critical intri...A main requirement for cells to function normally is the availability of glucose.Glucose,available either direct from circulation or storage,is converted to the essential energy that cells need to drive critical intrinsic functions.If cells are deprived of glucose,they become dysfunctional and suffer distress.Photobiomodulation,the use of specific wavelengths of light on body tissues,has been shown to promote,through small organelles called mitochondria,the metabolism of glucose to make energy for cells;this energy can be used to improve cell function and survival.In this perspective,we hypothesize that the availability of glucose is central to the core mechanism of photobiomodulation;that photobiomodulation is at its most efficient in stimulating mitochondrial activity and improving cell function when there is glucose readily available.展开更多
主题语境:濒危动物的保护篇幅:350词建议用时:7分钟1 Scientists are worried about the future of the axolotl,an unusual Mexican salamander,and theyre asking for help.A new program lets people“adopt”an axolotl to raise mo...主题语境:濒危动物的保护篇幅:350词建议用时:7分钟1 Scientists are worried about the future of the axolotl,an unusual Mexican salamander,and theyre asking for help.A new program lets people“adopt”an axolotl to raise money to support efforts to save the endangered animal.展开更多
文摘目的:探讨罗伊氏乳杆菌对肠上皮细胞炎症反应和凋亡的作用及相关机制。方法:通过免疫荧光和酶联免疫吸附检测罗伊氏乳杆菌对肠上皮细胞炎症反应和凋亡的影响;采用生物信息学分析、定量聚合酶链式反应和双荧光素酶报告基因等实验筛选罗伊氏乳杆菌发挥作用的下游微小核糖核酸(microRNA,miRNA);通过双荧光素酶报告基因和Western blot等实验筛选miR-196a下游靶基因及其功能。结果:罗伊氏乳杆菌能够抑制脂多糖引起的肠上皮细胞炎症反应和凋亡,进一步分析发现罗伊氏乳杆菌通过上调肠上皮细胞miR-196a抑制细胞炎症和凋亡,miR-196a通过靶向抑制程序性细胞死亡因子4(programmed cell death 4,PDCD4)基因表达发挥对肠上皮细胞的保护作用。结论:罗伊氏乳杆菌通过miR-196a靶向抑制PDCD4基因表达,减缓肠上皮细胞炎症反应和凋亡。
文摘A main requirement for cells to function normally is the availability of glucose.Glucose,available either direct from circulation or storage,is converted to the essential energy that cells need to drive critical intrinsic functions.If cells are deprived of glucose,they become dysfunctional and suffer distress.Photobiomodulation,the use of specific wavelengths of light on body tissues,has been shown to promote,through small organelles called mitochondria,the metabolism of glucose to make energy for cells;this energy can be used to improve cell function and survival.In this perspective,we hypothesize that the availability of glucose is central to the core mechanism of photobiomodulation;that photobiomodulation is at its most efficient in stimulating mitochondrial activity and improving cell function when there is glucose readily available.
文摘主题语境:濒危动物的保护篇幅:350词建议用时:7分钟1 Scientists are worried about the future of the axolotl,an unusual Mexican salamander,and theyre asking for help.A new program lets people“adopt”an axolotl to raise money to support efforts to save the endangered animal.
