Chronic pain, especially the chronic neuropathic pain syndrome characterized by long duration, slow recovery and difficulty to control in clinic practice makes it more serious physically and psychologically. Nowadays,...Chronic pain, especially the chronic neuropathic pain syndrome characterized by long duration, slow recovery and difficulty to control in clinic practice makes it more serious physically and psychologically. Nowadays, the field of chro nic neuropathic pain has been grasping more and more attention. The present stud y aims at investigating the optimal interval parameter of the electroacupunctur e (EA) treatment. L 5/L 6 nerve ligation model was used to assess the effect o f EA on neuropathic pain. Mechanical allodynia was assessed by 50% paw withdrawal t hreshold and cold induced ongoing pain was detected by the number of paw lifts within 5 minutes when free rat was put on to 5±1 ℃ cold plate. Han’s Acupoint Nerve Stimulator (HANS) was connected to needles inserted into acupo ints "Jiaji"(EX B 2/L 5) and "Weizhong" (BL 40) on both sides. Other parame ters were: intensity of 0.5～ 1 mA, 1～2 mA , ten minutes each; frequency of 2 H z; and 0.6 ms in pulse width. Different interval parameter was Q 1 day (one tr eatment/day), Q 2 day, Q 3 day and Q 4 day. Results: Optimal interval pla ys a critical r ole for the analgesic effect of electroacupuncture. The interval of Q 1 day ha s a declining analgesic effect or even not any effect. The other 3 groups have accumulative effect and long lasting analgesic effect (24 hours for mechanical allodynia and 1 week for cold induced ongoing pain). Compared with the control, there is a significant difference (P<0.01 or 0.001). Among these 4 in tervals, Q 3 day group is the best. Longer lasting analgesic effect following this interval choice (48 hours for mechanical allodynia and 10 days for cold i nduced ongoing pain). Amongst the 4 interval parameters, Q 3 day group has don e better effect than the others(P<0.01 or 0.001). The results indicate that one EA treatment every 3 days is the optimal interval. And it may have som ething to do with the clinical practice.展开更多
Acupuncture has been applied in the treatment of Parkinson’s Disease (PD) in China for over decades. Clinical practice revealed that it could relieve the suf ferings of PD patients and improve their life quality. Bes...Acupuncture has been applied in the treatment of Parkinson’s Disease (PD) in China for over decades. Clinical practice revealed that it could relieve the suf ferings of PD patients and improve their life quality. Besides, acupuncture is a lso effective for other neurological disorders such as optic nerve atrophy, spin al cord injury and brain dysplasia. But the mechanism underlying the phenomenon is still unknow n. In the present study, we gave high and low frequency electroacupuncture (EA) stimulation to the medial forebrain bundle (MFB) transected rats in order to fi nd whether EA could attenuate the abnormal rotational behavior and dopaminergic neuron degeneration or increase the gene expression of GDNF and BDNF in the dopa minergic neurons. The main findings are: ① Transection of the MFB with a wire k nife resulted in abnormal ipsilateral rotational behavior of rats induced by amp hetamine and reduction of DA in the striatum to about 50% two weeks after the tr ansection. ② Both chronic low (2 Hz) and high (100 Hz) frequency EA stimulation were effective in attenuating the rotational behavior within 2 weeks of MFB tra nsection. The same effect could last for at least 4 weeks in the animals stimula ted with high frequency EA but not low frequency EA. ③ The content of DA in the striatum was increased significantly in the unlesioned side of both the low and high frequency EA stimulated rats. No increase had been found in the lesioned s ide. ④ High frequency EA could increase the gene expression of GDNF on the unle sioned side of substantia nigra reticular (SNr) region, and both sides of the gl obus pallidus, while low frequency EA was effective in increasing gene expressio n of GDNF in the unlesioned side of globus pallidus. ⑤ High frequency EA could attenuate the degeneration of dopaminergic neurons in the SNc. The low frequency EA had no effect. ⑥ High frequency EA, but not the low frequency, could increa se the gene expression of BDNF in the SNc and the ventral tegmental area (VTA) o f the lesioned side of rats after MFB transection, indicating that high frequenc y EA stimulation could be more effective than the low frequency EA in accelerati ng the synthesis of endogenous neurotrophic factors and in attenuating the degen eration of dopaminergic neurons.展开更多
文摘Chronic pain, especially the chronic neuropathic pain syndrome characterized by long duration, slow recovery and difficulty to control in clinic practice makes it more serious physically and psychologically. Nowadays, the field of chro nic neuropathic pain has been grasping more and more attention. The present stud y aims at investigating the optimal interval parameter of the electroacupunctur e (EA) treatment. L 5/L 6 nerve ligation model was used to assess the effect o f EA on neuropathic pain. Mechanical allodynia was assessed by 50% paw withdrawal t hreshold and cold induced ongoing pain was detected by the number of paw lifts within 5 minutes when free rat was put on to 5±1 ℃ cold plate. Han’s Acupoint Nerve Stimulator (HANS) was connected to needles inserted into acupo ints "Jiaji"(EX B 2/L 5) and "Weizhong" (BL 40) on both sides. Other parame ters were: intensity of 0.5～ 1 mA, 1～2 mA , ten minutes each; frequency of 2 H z; and 0.6 ms in pulse width. Different interval parameter was Q 1 day (one tr eatment/day), Q 2 day, Q 3 day and Q 4 day. Results: Optimal interval pla ys a critical r ole for the analgesic effect of electroacupuncture. The interval of Q 1 day ha s a declining analgesic effect or even not any effect. The other 3 groups have accumulative effect and long lasting analgesic effect (24 hours for mechanical allodynia and 1 week for cold induced ongoing pain). Compared with the control, there is a significant difference (P<0.01 or 0.001). Among these 4 in tervals, Q 3 day group is the best. Longer lasting analgesic effect following this interval choice (48 hours for mechanical allodynia and 10 days for cold i nduced ongoing pain). Amongst the 4 interval parameters, Q 3 day group has don e better effect than the others(P<0.01 or 0.001). The results indicate that one EA treatment every 3 days is the optimal interval. And it may have som ething to do with the clinical practice.
文摘Acupuncture has been applied in the treatment of Parkinson’s Disease (PD) in China for over decades. Clinical practice revealed that it could relieve the suf ferings of PD patients and improve their life quality. Besides, acupuncture is a lso effective for other neurological disorders such as optic nerve atrophy, spin al cord injury and brain dysplasia. But the mechanism underlying the phenomenon is still unknow n. In the present study, we gave high and low frequency electroacupuncture (EA) stimulation to the medial forebrain bundle (MFB) transected rats in order to fi nd whether EA could attenuate the abnormal rotational behavior and dopaminergic neuron degeneration or increase the gene expression of GDNF and BDNF in the dopa minergic neurons. The main findings are: ① Transection of the MFB with a wire k nife resulted in abnormal ipsilateral rotational behavior of rats induced by amp hetamine and reduction of DA in the striatum to about 50% two weeks after the tr ansection. ② Both chronic low (2 Hz) and high (100 Hz) frequency EA stimulation were effective in attenuating the rotational behavior within 2 weeks of MFB tra nsection. The same effect could last for at least 4 weeks in the animals stimula ted with high frequency EA but not low frequency EA. ③ The content of DA in the striatum was increased significantly in the unlesioned side of both the low and high frequency EA stimulated rats. No increase had been found in the lesioned s ide. ④ High frequency EA could increase the gene expression of GDNF on the unle sioned side of substantia nigra reticular (SNr) region, and both sides of the gl obus pallidus, while low frequency EA was effective in increasing gene expressio n of GDNF in the unlesioned side of globus pallidus. ⑤ High frequency EA could attenuate the degeneration of dopaminergic neurons in the SNc. The low frequency EA had no effect. ⑥ High frequency EA, but not the low frequency, could increa se the gene expression of BDNF in the SNc and the ventral tegmental area (VTA) o f the lesioned side of rats after MFB transection, indicating that high frequenc y EA stimulation could be more effective than the low frequency EA in accelerati ng the synthesis of endogenous neurotrophic factors and in attenuating the degen eration of dopaminergic neurons.
