患者,男性,58岁,乙型肝炎后肝硬化失代偿期、2型糖尿病12年,近1个月再发腹胀、水肿,尿少入院。住院期间患者寒战、发热,取血标本进行培养,选用头孢哌酮/舒巴坦经验性抗感染治疗。确诊非O1/O139霍乱弧菌引起的血流感染,药敏试验提示头孢...患者,男性,58岁,乙型肝炎后肝硬化失代偿期、2型糖尿病12年,近1个月再发腹胀、水肿,尿少入院。住院期间患者寒战、发热,取血标本进行培养,选用头孢哌酮/舒巴坦经验性抗感染治疗。确诊非O1/O139霍乱弧菌引起的血流感染,药敏试验提示头孢哌酮/舒巴坦敏感后,继续使用当前药物,头孢哌酮/舒巴坦由3 g q12h改为3 g q8h治疗。10 d后患者无发热,血培养和粪便霍乱弧菌培养阴性,好转出院。展开更多
Objective To construct the detection method of vibrio cholerae with fluorescence quantitative PCR and evaluate its superiority.Methods A diagnostic kit(FQ-PCR) was developed according to the common sequence of NhaA ge...Objective To construct the detection method of vibrio cholerae with fluorescence quantitative PCR and evaluate its superiority.Methods A diagnostic kit(FQ-PCR) was developed according to the common sequence of NhaA gene in vibrio cholerae.The sensitivity and speciality in detecting of vibrio cholerae in infectious water,sea food and winter-endurance specimen and clinical samples were compared with FQ-PCR kit.Results In the detection of clinical samples,FQ-PCR and regular detection ways have the same sensitivity and speciality.While in the detection of infectious water,sea food and winter-endurance specimen,FQ-PCR has superiority to regular ways.Conclusion The construction of FQ-PCR method eliminates PCR cross-contamination which cause false positive,and real-time detection ensure accurate quantity.So FQ-PCR can be used to the detection of vibrio cholerae in clinical and sanitary laboratory.展开更多
文摘患者,男性,58岁,乙型肝炎后肝硬化失代偿期、2型糖尿病12年,近1个月再发腹胀、水肿,尿少入院。住院期间患者寒战、发热,取血标本进行培养,选用头孢哌酮/舒巴坦经验性抗感染治疗。确诊非O1/O139霍乱弧菌引起的血流感染,药敏试验提示头孢哌酮/舒巴坦敏感后,继续使用当前药物,头孢哌酮/舒巴坦由3 g q12h改为3 g q8h治疗。10 d后患者无发热,血培养和粪便霍乱弧菌培养阴性,好转出院。
文摘Objective To construct the detection method of vibrio cholerae with fluorescence quantitative PCR and evaluate its superiority.Methods A diagnostic kit(FQ-PCR) was developed according to the common sequence of NhaA gene in vibrio cholerae.The sensitivity and speciality in detecting of vibrio cholerae in infectious water,sea food and winter-endurance specimen and clinical samples were compared with FQ-PCR kit.Results In the detection of clinical samples,FQ-PCR and regular detection ways have the same sensitivity and speciality.While in the detection of infectious water,sea food and winter-endurance specimen,FQ-PCR has superiority to regular ways.Conclusion The construction of FQ-PCR method eliminates PCR cross-contamination which cause false positive,and real-time detection ensure accurate quantity.So FQ-PCR can be used to the detection of vibrio cholerae in clinical and sanitary laboratory.
