Changes of intracellular Ca 2+ ,pH value and mitochondria membrane potential(△Ψ m) in the apoptosis of MGC 803 cells induced by water soluble constituents of Glycyrrhiza uralensis Fisch(WSCG) were investigated.MGC 8...Changes of intracellular Ca 2+ ,pH value and mitochondria membrane potential(△Ψ m) in the apoptosis of MGC 803 cells induced by water soluble constituents of Glycyrrhiza uralensis Fisch(WSCG) were investigated.MGC 803 cells were incubated with 0.5,0.75,1.0 and 1.5 g/L WSCG for 1,4,7,11,15,19 and 23 hours respectively.The percentage of apoptosis and intracellular Ca 2+ content increased in a dose and time dependent manner,except that the intracellular Ca 2+ content of 1.5 g/L WSCG treated cells started to decrease after 11 hours.The cells were alkalized after various treatments,except that 1.5 g/L WSCG treated cells turned to acidify after 11 hours.It was found that the mitochondria △Ψ m of all treated cells decreased drastically at the first hour,then kept decreasing slowly until the 23rd hour.The results indicated that intracellular Ca 2+ ,pH and mitochondria △Ψ m all played pivotal roles in the apoptosis of MGC 803 cells induced by WSCG.展开更多
为了准确计算除尘变压器的铁芯损耗,在分析传统的有限元分析方法以及Steinmetz公式的基础上,提出了一种计算除尘变压器铁芯损耗的棱边元与节点元相结合的E??法模型;并以一台20 k Hz、50 k V、60 k W除尘变压器为例,建立了三维模型,计算...为了准确计算除尘变压器的铁芯损耗,在分析传统的有限元分析方法以及Steinmetz公式的基础上,提出了一种计算除尘变压器铁芯损耗的棱边元与节点元相结合的E??法模型;并以一台20 k Hz、50 k V、60 k W除尘变压器为例,建立了三维模型,计算了变压器的铁芯损耗。将利用该方法计算的出的铁芯损耗计算结果同实测值、节点有限元、棱边有限元以及Steinmetz公式计算结果进行对比。对比结果表明:该方法计算精度明显高于Steinmetz公式模型、棱边有限元法以及节点有限元法的计算结果。证明了该算法能够更加准确的计算变压器铁芯损耗。展开更多
文摘Changes of intracellular Ca 2+ ,pH value and mitochondria membrane potential(△Ψ m) in the apoptosis of MGC 803 cells induced by water soluble constituents of Glycyrrhiza uralensis Fisch(WSCG) were investigated.MGC 803 cells were incubated with 0.5,0.75,1.0 and 1.5 g/L WSCG for 1,4,7,11,15,19 and 23 hours respectively.The percentage of apoptosis and intracellular Ca 2+ content increased in a dose and time dependent manner,except that the intracellular Ca 2+ content of 1.5 g/L WSCG treated cells started to decrease after 11 hours.The cells were alkalized after various treatments,except that 1.5 g/L WSCG treated cells turned to acidify after 11 hours.It was found that the mitochondria △Ψ m of all treated cells decreased drastically at the first hour,then kept decreasing slowly until the 23rd hour.The results indicated that intracellular Ca 2+ ,pH and mitochondria △Ψ m all played pivotal roles in the apoptosis of MGC 803 cells induced by WSCG.
文摘为了准确计算除尘变压器的铁芯损耗,在分析传统的有限元分析方法以及Steinmetz公式的基础上,提出了一种计算除尘变压器铁芯损耗的棱边元与节点元相结合的E??法模型;并以一台20 k Hz、50 k V、60 k W除尘变压器为例,建立了三维模型,计算了变压器的铁芯损耗。将利用该方法计算的出的铁芯损耗计算结果同实测值、节点有限元、棱边有限元以及Steinmetz公式计算结果进行对比。对比结果表明:该方法计算精度明显高于Steinmetz公式模型、棱边有限元法以及节点有限元法的计算结果。证明了该算法能够更加准确的计算变压器铁芯损耗。
