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灵发素(LFS)对诱导半夏体细胞胚胎发生过程中内源激素含量的影响 被引量:3
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作者 梁琼月 周凤珏 +3 位作者 许鸿源 覃柳兰 何丽珊 何冰 《热带农业科学》 2017年第2期38-42,共5页
以半夏叶柄为材料,比较MS+BA 2.0 mg/L+NAA 0.1 mg/L(CK)和MS+LFS 0.3 mg/L(Lingfasu,LFS,灵发素)2种培养基在诱导体细胞胚胎发生和发育过程中内源激素的变化差异。结果表明:在半夏体细胞胚胎发生和发育过程中,2个处理组内源激素变化趋... 以半夏叶柄为材料,比较MS+BA 2.0 mg/L+NAA 0.1 mg/L(CK)和MS+LFS 0.3 mg/L(Lingfasu,LFS,灵发素)2种培养基在诱导体细胞胚胎发生和发育过程中内源激素的变化差异。结果表明:在半夏体细胞胚胎发生和发育过程中,2个处理组内源激素变化趋势,除ABA大致呈"M"状外,IAA、GA_3和ZR激素大致呈"∧"状;在半夏体胚发生的重要时期(胚性愈伤组织时期和早期胚时期),LFS处理组中IAA和ABA的含量显著高于CK组,ZR在早期胚时期高于对照29.0%,GA_3含量以CK组含量较高。此结果表明,LFS对半夏体细胞胚胎的发生和发育过程中内源激素IAA、ZR、ABA和GA_3的调控起着重要作用。 展开更多
关键词 半夏 灵发素(lfs) 体细胞胚胎 内源激素
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基于PCR和LFS技术的掺假豌豆成分快速检测
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作者 王凯 屈玮 +2 位作者 姚帮本 徐建国 姚丽 《合肥工业大学学报(自然科学版)》 CAS 北大核心 2022年第11期1575-1579,共5页
文章运用聚合酶链式反应(polymerase chain reaction,PCR)和侧向流试纸条(lateral flow strip,LFS)相结合方法,以豌豆特异性基因为参照对绿豆食品中掺入豌豆成分进行定性分析。实验对退火温度、修饰引物浓度和镁离子浓度进行优化,并在最... 文章运用聚合酶链式反应(polymerase chain reaction,PCR)和侧向流试纸条(lateral flow strip,LFS)相结合方法,以豌豆特异性基因为参照对绿豆食品中掺入豌豆成分进行定性分析。实验对退火温度、修饰引物浓度和镁离子浓度进行优化,并在最优PCR条件下进行特异性和灵敏度检测。结果表明,在退火温度为59℃,修饰引物加入量为0.20μmol/L,镁离子加入量为1.75 mmol/L条件下,PCR扩增效果最好,掺假检测限为0.5%,最低可检测豌豆DNA量为0.12 ng。该方法特异性高、操作简单快速,依靠视觉观察就能得出定性结果。 展开更多
关键词 绿豆产品 豌豆掺假 聚合酶链式反应(PCR) 侧向流试纸条(lfs)
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日志结构云存储中缓存的设计与实现 被引量:6
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作者 陈莉君 王森 康华 《西安邮电大学学报》 2013年第5期76-80,共5页
日志结构分布式文件系统(HDFS-based Log-structured File System,HLFS)是基于Hadoop分布式文件系统(Hadoop Distributed File System,HDFS)构建的云存储平台,为了提高其数据读写性能,结合HLFS客户端与后台存储集群相分离的特点,利用最... 日志结构分布式文件系统(HDFS-based Log-structured File System,HLFS)是基于Hadoop分布式文件系统(Hadoop Distributed File System,HDFS)构建的云存储平台,为了提高其数据读写性能,结合HLFS客户端与后台存储集群相分离的特点,利用最近最久未使用算法作为缓存策略,在HLFS客户端内存中设计并实现HLFS缓存,并采用固定时间间隔刷新缓存的策略来保证数据一致性。在集群系统上对所设计的缓存进行了数据读写测试,结果表明,该设计能够有效地降低HLFS与后台集群的交互频率,减少用户感知的数据读写时间。 展开更多
关键词 分布式文件系统(DFS) 缓存技术 日志结构文件系统(lfs)
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Rapid and easy determination of morphine in chafing dish condiments with colloidal gold labeling based lateral flow strips 被引量:4
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作者 Wei Chen Xin-ni Li +2 位作者 Qian Wu Li Yao Jianguo Xu 《Food Science and Human Wellness》 SCIE 2019年第1期40-45,共6页
In order to enhance the flavor of chafing dish and increase the attraction of consumers,the poppy shell is reported to be illegally added to the condiments of chafing dish.In this research,a rapid,simple,and convenien... In order to enhance the flavor of chafing dish and increase the attraction of consumers,the poppy shell is reported to be illegally added to the condiments of chafing dish.In this research,a rapid,simple,and convenient method based on the classic immunochromatographic lateral flow strip(LFS)with gold nanoparticles(GNPs)labeling was developed for easy monitoring of morphine(MOP),an effective component of poppy shell.Under optimized conditions,this developed LFS can well realize the detection of target MOP in the condiments of chafing dish in less than 10 min without any complicated pre-treatments.The limit of detection(LOD)can be achieved as low as 0.1 ppb for standard MOP or the MOP spiked condiments of chafing dish.All these results of the research strongly demonstrate that this established LFS method can be successfully applied in practical rapid and accurate on-site screening of poppy shell in condiments of chafing dish. 展开更多
关键词 Morphine(MOP) Gold nanoparticles(GNPs) Lateral flow strip(lfs) Chafing dish condiments
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Numerical modeling of time-dependent deformation and induced stresses in concrete pipes constructed in Queenston shale using micro-tunneling technique 被引量:1
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作者 Hayder Mohammed Salim Al-Maamori M.Hesham El Naggar Silvana Micic 《Journal of Rock Mechanics and Geotechnical Engineering》 SCIE CSCD 2018年第2期290-309,共20页
Effects of time-dependent deformation(TDD) on a tunnel constructed using the micro-tunneling technique in Queenston shale(QS) are investigated employing the finite element method. The TDD and strength parameters o... Effects of time-dependent deformation(TDD) on a tunnel constructed using the micro-tunneling technique in Queenston shale(QS) are investigated employing the finite element method. The TDD and strength parameters of the QS were measured from tests conducted on QS specimens soaked in water and lubricant fluids(LFs) used in micro-tunneling such as bentonite and polymer solutions. The numerical model was verified using the results of TDD tests performed on QS samples, field measurements of some documented projects, and the closed-form solutions to circular tunnels in swelling rock. The verified model was then employed to conduct a parametric study considering important micro-tunneling design parameters, such as depth and diameter of the tunnel, in situ stress ratio(K;), and the time lapse prior to replacing LFs with permanent cement grout around the tunnel. It was revealed that the time lapse plays a vital role in controlling deformations and associated stresses developed in the tunnel lining.The critical case of a pipe or tunnel in which the maximum tensile stress develops at its springline occurs when it is constructed at shallow depths in the QS layer. The results of the parametric study were used to suggest recommendations for the construction of tunnels in QS employing micro-tunneling. 展开更多
关键词 Numerical model Micro-tunneling Queenston shale(QS) Lubricant fluids(lfs)
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Landfill Sites Identification Using GIS and Multi-Criteria Method: A Case Study of Intermediate City of Punjab, Pakistan 被引量:1
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作者 Adeel Ahmad Umar Javaid +3 位作者 Muhammad Asif Javed Sajid Rashid Ahmad Muhammad Abbas Jaffri Mudassar Ashfaq 《Journal of Geographic Information System》 2016年第1期40-49,共10页
Escalation in population over time triggered the abrupt exploitation of natural resources for human survival trough industrialization that ultimately caused splendid increase in the waste generation. This industrial d... Escalation in population over time triggered the abrupt exploitation of natural resources for human survival trough industrialization that ultimately caused splendid increase in the waste generation. This industrial development resulted in the migration of rural community toward urban areas. Management of urban waste is a great challenge for the urban administration. However, technologies have been developed to manage the waste in environmental friendly and sustainable manner. Sanitary landfill sites are one of the latest methods of disposing the municipal solid waste in an environment friendly and sustainable manner. Government and administrative authorities are adopting this technology for the management of urban solid waste. Present study is about identifying landfill sites for the Sahiwal city with an area of 1160 square kilometer and projected population of 1.57 million persons in 2016. Geographical Information System (GIS) is used for the identification of appropriate landfill site (LFS) that can fulfill the need of city in future and is selected based on the sustainable and eco-friendly criteria. The criteria are developed keeping in view the proximity from several land-use features i.e. water bodies, roads, settlements, agricultural land, bare land and existing disposal sites. The weights of the criteria are quantified using pair-wise comparison method in Analytic Hierarchy Process (AHP). The weights are incorporated in GIS spatial data environment and are assigned to proximity threshold of each criterion. Ultimately a map for each criterion is developed highlighting suitable, least suitable, less suitable and un-suitable areas with respect to each specific criterion. These maps are spatially overlaid which result in a final map that identifies most suitable landfill sites for solid waste disposal. The five identified sites are then prioritized based on their distances from the city center and area available. All identified sites are on the bare land and contain considerable buffer from environmentally sensitive receptors. 展开更多
关键词 URBANIZATION Urban Waste Landfill Site (lfs) GIS Analytic Hierarchy Process (AHP)
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Filtration assisted pretreatment for rapid enrichment and accurate detection of Salmonella in vegetables
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作者 Bin Li Hanling Wang +5 位作者 Jianguo Xu Wei Qu Li Yao Bangben Yao Chao Yan Wei Chen 《Food Science and Human Wellness》 SCIE CSCD 2023年第4期1167-1173,共7页
Rapid detection of target foodborne pathogens plays more and more significant roles in food safety,which requires the efficiency,sensitivity,and accuracy.In this research,we proposed a new st rategy of isothermal-mole... Rapid detection of target foodborne pathogens plays more and more significant roles in food safety,which requires the efficiency,sensitivity,and accuracy.In this research,we proposed a new st rategy of isothermal-molecular-amplification integrated with lateral-flow-strip for rapid detection of Salmonella without traditional enrichment-culture.Th e designed syringe-assisted-filtration can contribute to simultaneous collection and concentration of target bacterium from vegetable samples in just 3 min,resolving the drawbacks of traditional random sampling protocols.After simple and convenient ultrasonication,samples can be directly amplified at 39℃ in 25 min and the amplicons are qualitatively and quantitatively analyzed with the designed lateral-flow-strip in 5 min.Finally,satisfied results have been achieved within 40 min,which greatly improve the efficiency while the accuracy is also guaranteed.Furthermore,all detection steps can be completed under instrument-free conditions.This method will hold great promise for target pathogen detection in the resource-limited district,or for emergency on-site identification. 展开更多
关键词 VEGETABLES SALMONELLA Filtration enrichment Culture-free detection Enzymatic recombinase amplification(ERA) Lateral flow strip(lfs) Rapid detection Food safety
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机载海洋溢油探测任务系统 被引量:1
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作者 王根成 高超 《工业技术创新》 2020年第3期97-101,共5页
比较分析了海洋溢油探测的主要传感器,提出了基于合成孔径雷达(SAR)、激光荧光传感器(LFS)、船载自动识别系统(AIS)的机载海洋溢油探测任务系统。机动探测主要探测的内容包括航路、油井及事故突发海域溢油位置、面积、油量、种类等数据... 比较分析了海洋溢油探测的主要传感器,提出了基于合成孔径雷达(SAR)、激光荧光传感器(LFS)、船载自动识别系统(AIS)的机载海洋溢油探测任务系统。机动探测主要探测的内容包括航路、油井及事故突发海域溢油位置、面积、油量、种类等数据,并动态监视油膜运动趋势。分析了载荷、通信、地面保障、信息处理4个分系统,研究了AIS接收机(带安装架)、AIS天线的信息处理功能。实例验证表明该系统易于安装、成本低、实用性强。 展开更多
关键词 合成孔径雷达(SAR) 激光荧光传感器(lfs) 船载自动识别系统(AIS) 机载 海洋 溢油探测
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