Low Level of Cystic Fibrosis Transmembrane Conductance Regulator Is Associated with Human Sperm Autophagy and Vitality

Low sperm motility is one of the main causes of male infertility. Cystic fibrosis transmembrane conductance regulator (CFTR, an anion channel protein) is related to the progressive motility of sperm. CFTR disruptor CFTRinh-172 or forskolin (FSK) in this study were used to treat human sperm separately, and the rates of sperm autophagy and progressive motility, mitochondrial membrane potential (MMP) and ATP concentration, and the expression levels of related factors were detected to explore their relationship. It was showed that sperms treated with CFTRinh-172 or FSK reduced the levels of cAMP, CFTR and PKA, but increased sperm autophagy rate, expression levels of AMPK and LC3B. However, reactive oxygen species content had no significant difference. It was indicated that low level of CFTR performed with cAMP and its downstream effectors such as PKA and AMPK to regulate mitochondrial structure and function, leading to increased autophagy rate and reduced vitality of sperm.

LASAR仿真中的VITAL建模方法研究

应用LASAR仿真方法时,只有具备了电路板网络表和电路板上所有数字逻辑器件的模型,才能够生成有效的电路板测试数据。构建完整的器件库,是有待研究的1项重要内容。VITAL建模是众多建模方法中较科学并容易实现的途径,它为ASIC库的建立、电路设计的描述提供了便利的、格式相对固定的描述方法。本文介绍VITAL的基本内容,并对用VITAL建立电路模型的方法进行详细描述。

以“VITAL”为核心理念的建筑类专业国际化人才培养模式构建与实践

培养高水平国际化人才、助推“双一流”建设已成为发展我国高等教育的重要组成部分。近年来建筑学科国际化人才培养虽已取得长足发展,但审视建筑学科发展后,仍存在国际化专业知识结构单一、与国家和行业发展需求关联不足、国际化与本土化教育缺乏良性互动等问题。重庆大学建筑城规学院围绕国家一流专业建设,坚持立德树人,秉承“高水平、高标准、国际化”的人才培养目的,以创新能力、实践能力、国际化能力、科研能力等关键能力培养为主线,构建实施了以“VITAL”为核心理念的国际化建筑类人才培养体系,并与国外一流建筑院校建立长期稳定合作关系,拓展了学生国际化学科视野,为培养一批在建筑类学科领域兼有国际视野与本土情怀、创新意识与批判性思维的国际化人才提供了条件。

基于Android Vitals的应用性能监控方法

随着移动应用的普及,应用的性能成为了用户体验的重要因素。文章提出了一种基于Android Vitals的应用性能监控方法,该方法可以监控应用的性能指标,如应用启动时间、响应时间和内存占用等,通过收集和分析这些数据,可以对应用的性能进行评估和优化。利用Android系统自带的性能监控工具和第三方工具来收集数据,并通过可视化工具展示结果,从而为开发人员提供了一种简单、有效的性能监控方法。实验结果表明,该方法可以对应用的性能进行准确地评估和优化,对提高应用的用户体验具有重要意义。

Vitality variation and population structure of a riparian forest in the lower reaches of the Tarim River,NW China

Since 2000, the Chinese government has implemented emergency water diversion measures to restore the damaged riparian forest ecosystem with dominant tree species Euphrat poplar(Populus euphratica Oliv.)at the lower reaches of the Tarim River. In the present study, comparative analysis of variations in the vitality of P. euphratica trees were made using 2005 and 2010 data to illustrate the revitalization process of riparian forest. Poplar trees within 300 m of the riverbed were positively revitalized, while the vitality of trees farther than 300 m from the river decreased. Population structure was studied to demonstrate the development of poplar community. In the first belt, the class structure for the diameter at breast height(DBH) of P. euphratica fit a logistic model, and the 2nd, 3rd and 4th belt curve fittings were close to a Gaussian model; in other plots they were bimodal. Cluster analysis of the composition of the DBH class of poplar trees demonstrated that those within 16–36 cm DBH were the most abundant(58.49% of total) in study area, under 16 cm of DBH were second(31.36%), and trees >40 cm DBH were the least abundant(10.15%). More than 80% of the trees were young and medium-sized, which means that the poplar forest community in the vicinity of the lower Tarim River is at a stable developmental stage. The abundance of juvenile trees of P. euphratica in the first and second measuring belts was 12.13% in 2005 and increased to 25.52% in 2010, which means that the emergency water transfer had a positive impact on the generation of young P. euphratica trees in the vicinity of the river.

Detecting overlapping communities based on vital nodes in complex networks

Detection of community structures in the complex networks is significant to understand the network structures and analyze the network properties. However, it is still a problem on how to select initial seeds as well as to determine the number of communities. In this paper, we proposed the detecting overlapping communities based on vital nodes algorithm（DOCBVA）, an algorithm based on vital nodes and initial seeds to detect overlapping communities. First, through some screening method, we find the vital nodes and then the seed communities through the pretreatment of vital nodes. This process differs from most existing methods, and the speed is faster. Then the seeds will be extended. We also adopt a new parameter of attribution degree to extend the seeds and find the overlapping communities. Finally, the remaining nodes that have not been processed in the first two steps will be reprocessed. The number of communities is likely to change until the end of algorithm. The experimental results using some real-world network data and artificial network data are satisfactory and can prove the superiority of the DOCBVA algorithm.

EFFECTS OF DIFFERENT COMBINATIONS OF CRYOPROTECTANTS ON SPERMATOZOON VITALITY OF CHINESE SCALLOP,CHLAMYS FARRERI,DURING LOW TEMPERATURE EQUILIBRIUM

The effects of membrane penetrating anti-freezing agents(MPAAs), DMSO(dimethyl sulfoxide),glycerol,EG(ethylene glycol) and methanol in combination with different cryoprotective additives suchas carbohydrates,macromolecules and inorganic compounds on the spermatozoon vitality of Chinesescallop,Chlamys farreri,during 1 h 0℃ equilibrium were investigated.When only MPAAs existed,the detrimental effects of different MPAAs ranked in the following order:DMSO【methanol【EG【glycerol.When carbohydrates were added into MPAAs solution,5% glucose caused larger decrease ofspermatozoon vitality than 2.4% lactose.5% glucose or 2.4% lactose in 7.5% glycerol caused com-plete damage.10% yolk was best in maintaining the spermatozoon vitality except when used incombination with 10% methanol.10% milk significantly decreased spermatozoon vitality in EG andmethanol and enhanced its vitality in glycerol,but did not significantly influence it in DMSO.Glycinewas apparently detrimental to spermatozoon vitality.The average

Impact of Smoking on Lifestyle and Vitality in College Students

There is a long history of studies on the bad influence of smoking on our bodies, with many study results accumulated to date indicating a decline in the smoking rate. However, the smoking rate in Japan is still higher than that in other countries. In order to reveal the various influences due to smoking, we examined the changes in lifestyle and vitality prior to and the following smoking, targeting college students who are at the early age of starting to smoke. The subjects were 213 college students (83 men and 130 women). Smoking frequency as well as sleeping, eating habits, exercise, and vitality as a lifestyle were surveyed through a questionnaire survey. As a result of comparing smoking and lifestyle between the smoking group and non-smoking group, in terms of sleep, the bedtime and wake-up time tended to be late in the smoking group. Regarding eating habits, there was a tendency for the smoking group to have a high rate of skipping breakfast and a high intake rate of midnight snacks. Regarding exercise, the number of people exercising on a daily basis was small in both the smoking group and the non-smoking group, with no significant difference observed. Therefore, this study revealed that smoking has a negative influence on some aspects of lifestyle. There was no significant relationship in terms of the effects of smoking on vitality. Going forward, it will be necessary to clarify the negative effects of smoking on mental health.

Typology-based Vitality Promotion of Urban Waterfront Public Space in the Three Gorges Reservoir

Revitalization of waterfront-a precious resource of city has gradually become popular worldwide. Cities around the Three Gorges Reservoir grow and proper for the Yangtze River,thus waterfront in these cities has been long ignored or faced with "constructive destruction" with the city development. This paper divided urban waterfront public spaces around the Three Gorges into 3 kinds of spaces,namely point,line and surface,from the perspective of typology,and put forward strategies such as functional renovation,and improvement of spatial scale from the perspectives of land use and traffic,to enhance the vitality of waterfront public space.

An approach to a non-contact vital sign monitoring using dual-frequency microwave radars for elderly care

This study aimed to produce a prototype system for non-contact vital sign monitoring of the elderly using microwave radar with the intention of reducing the burdens on monitored individuals and nursing caregivers. In addition, we tested the ability of the proposed prototype system to measure the respiratory and heart rates of the elderly in a nursing home and discussed the systems effectiveness and problems by examining results of real-time monitoring. The prototype system consisted of two 24-GHz microwave radar antennas and an analysis system. The antennas were positioned below a mattress to monitor motion on the body surface for measuring cardiac and respiratory rates from the dorsal side of the subjects (23.3 ± 1.2 years) who would be lying on the mattress. The heart rates determined by the prototype system correlated significantly with those measured by electrocardiography (r = 0.92). Similarly, the respiratory rates determined by the prototype correlated with those obtained from respiration curves (r = 0.94). Next, we investigated the effectiveness of the prototype system with 7 elderly patients (93.3 ± 10.56 years) at a nursing home. The proposed system appears to be a promising tool for monitoring the vital signs of the elderly in a way that alleviates the need to attach electrodes overnight to confirm patient safety.

基于VITAL的无人机目标跟踪方法

针对无人机飞行环境复杂及现有跟踪算法在精确性和实时性上略微不足的问题,提出了一种新型的基于VITAL的无人机目标跟踪算法.首先,设计一套完整的无人机目标跟踪控制系统;其次,对跟踪算法进行设计,将基于对抗学习的VITAL目标识别算法引入无人机目标跟踪算法,用EKF算法对其进行优化,提高实时性且防止目标跟踪失败;最后,通过实际跟踪和性能测试实验,得到相比主流的DNN等目标跟踪算法,算法在精确度上提高4%、实时性提高43%,算法在无人机目标跟踪方面具有较强的鲁棒性.

Vital Stim电刺激配合Mendelssohn吞咽法治疗脑卒中患者咽期吞咽障碍的疗效观察

目的:观察Vital Stim电刺激配合门德尔松(Mendelssohn)吞咽法治疗脑卒中患者咽期吞咽障碍的临床疗效。方法:选取我院72例脑卒中合并咽期吞咽障碍的患者,随机分为对照组和观察组,每组36例,对照组采用吞咽障碍的常规治疗和Vital Stim电刺激,观察组在对照组的基础上配合门德尔松(Mendelssohn)吞咽法,2组均连续治疗3周,观察两组治疗前后洼田饮水试验分级情况,标准吞咽功能评估量表(SSA)评分情况。结果:治疗前各项评估等级在两组之间无显著差异(P>0.05),治疗后观察组在洼田饮水试验评估中的总有效率为88.89%,优于对照组总有效率(69.44%),两组差异有统计学意义(P<0.05);两组患者的洼田饮水试验和SSA评估等级均有所下降,且观察组下降优势较对照组有显著性(P<0.05)。结论:Vital Stim电刺激同时配合门德尔松(Mendelssohn)吞咽法治疗脑卒中患者咽期吞咽障碍的疗效更佳。

MEDICAL ACUPUNCTURE IN SPORTS MEDICINE:RELEVANT POINTS AND THE POST-TRAUMATIC VITAL ALIGNMENT PROCEDURE

Medical acupuncture is an extremely useful therapeutic modality in sportsmedicine.There are a number of cardinal points that have proven effective in treating the pain associated with various kinds of sports injury and for alleviating the general discomfort associated with theseinjuries and with any particular illness an athlete may be experiencing.The pain and discomfort experienced by athletes also has mental and spiritual dimensions,and the post-traumatic vital alignment procedure I have developed helps atheletes recover a sense of well-being and″groundedness″after a serious injury or illness has disrupted their total,healthy equilibrium.

EFFECT OF LOW TEMPERATURE ON POLLEN VITALITY OF LARIX OLGENSIS

After the treatment of pollen of larix olgensis at the different low temperature, the pollen vitality was observed by using fluorescent staining method. Several conclusions were obtained: 1) 0℃-3℃ is the best temperature range for storing pollen of Larix olgensis. 2) At the same temperature, humidity is the key factor influencing the pollen vitality. 3) Different clones shows different resistance to low temperature. Among the ten clones 2# clone shows the highest resistance.

Effects of Seed Vitality and Regeneration on Genetic Integrity in Soybean by SSR Markers

The seeds of Zhonghuang 18 were selected as a test material,and subjected to artificial aging treatment(0,112,154 and 196 d),obtaining four 4 populations,i.e.,G_0-1,G_0-2,G_0-3 and G_0-4,the germination rates of which were 98.0%,95.0%,81.0%and 79.0%,respectively.The four populations were reproduced twice in field,giving four populations of the first reproduced generation G_1-1,G_1-2,G_1-3 and G_1-4 and four populations of the second reproduced generation G_2-1,G_2-2,G_2-3 and G_2-4.The results showed that the number of alleles(Ae)per locus and genetic identity of all the treatment populations did not change significantly compared with the control population G_0-1,and population G_0-4 still shared 0.996 2 genetic identity with the control population,indicating that the genetic identity between the population with a germination rate of 79.0%and the control population was still high.The results of t test showed that populations G_0-2,G_1-1 and G_2-1 showed number of alleles per locus(A),genetic diversity index(H)and Shannon index without significantly differences from the control population G_0-1;populations G_1-2 and G_2-2 had the number of alleles per locus(A)significantly decreased;and the above genetic diversity parameters of populations G_0-3,G_0-4,G_1-3,G_1-4,G_2-3 and G_2-4 decreased significantly or very significantly.The results ofχ~2test showed that there were almost no differences in the allelic frequency distribution between populations G_0-2,G_1-1 and G_2-1 and the control populaiton G_0-1;and populations G_0-3,G_0-4,G_1-2,G_1-3,G_1-4,G_2-2,G_2-3and G_2-4 differed in allele frequency distribution,and the lower the vitality level,the greater the differences.Compared with the control population G_0-1,populations G_0-2,G_1-1 and G_2-1 had no significant changes in number of rare alleles,while populations G_0-3,G_0-4,G_1-2,G_2-2,G_1-3,G_1-4,G_2-3 and G_2-4 decreased significantly in number of rare alleles.The above results showed that compared with the control population,the progeny populations reproduced from the population with a germination rate of 98.0%had significant changes in genetic diversity and number of rare alleles,while the values of the progeny populations reproduced from populations having germination rates of 81.0%and 79.0%,respectively,decreased significantly,and the number of alleles per locus and number of rare alleles of the progeny populations reproduced from the population with a germination rate of 95.0%began to decrease.The decline in viability has a greater effect on the genetic structure of soybean germplasm populations than reproduction generation.It is recommended that the germination rate standard for regeneration of soybean germplasm with an initial germination rate of 98.0%should not be lower than 81.0%.

Normal reference value of forced vital capacity of Chinese younger women and geographical factors

Objective To supply a scientific basis for standardizing the normal reference value of forced vital capacity(FVC)of Chinese younger women.Methods We studied the relationship between the normal reference value of 21767 samples of FVC of younger women and eight geographical factors in 157 areas in China.Results It was found that the correlation between geographical factors and the normal reference value of FVC of younger women was quite significant(F=5.884,P=0.000).By adopting the method of mathematical regression analysis,one regression equation was inferred:=3.146+0.00006919X1+0.01315X4-0.006966X6+0.09524X8±0.254.In the above equation,is the normal reference value of younger women’ FVC(L);X1 is the altitude(m);X4 is the annual mean air temperature(℃);X6 is the annual mean relative humidity(%);X8 is the annual mean wind speed(m/s);0.254 is the value of the residual standard deviation.Conclusion If geographical values are obtained in a certain area,the normal reference value of FVC of younger women in this area can be obtained by using the regression equation.Furthermore,according to the geographical factors,China can be divided into eight regions:Northeast China Region,North China Region,Shanxi-Shaanxi-Inner Mongolia Region,middle and lower reaches of the Yangzte River Region,Southeast China Region,Northwest China Region,Southwest China Region,and Qinghai-Tibet Plateau Region.

A neural network-based infection screening system that uses vital signs and percutaneous oxygen saturation for rapid screening of patients with influenza

Objective: Influenza is a highly infectious viral disease, which occurs epidemically almost every winter in Japan. Rapid screening of patients with suspected influenza in places of mass gathering is important to delay or prevent transmission of the infection. The aim of this study was to assess the effectiveness of our newly developed infection screening system that employed vital signs and percutaneous oxygen saturation (SpO2) as parameters in a clinical setting. Methods: Since SpO2 accurately reflects respiratory status during influenza virus infection, we upgraded our previous system by adding SpO2 as a new parameter to improve the screening accuracy. This system instantly measures SpO2 and vital signs (i.e., heart rate, respiration rate, and facial temperature), which automatically detects infected individuals via a neural network-based nonlinear discriminant function using these derived parameters. We tested the system on 45 patients with seasonal influenza (35.8℃ < body temperature < 40.0℃, 18-35 years) and 64 normal control subjects (35.0℃ < body temperature < 37.5℃, 18-30 years) at Japan Self-Defense Central Hospital in 2012. Results: The system identified 40/45 patients with influenza and 60/64 normal control subjects, and provided sensitivity, specificity, and positive and negative predictive value (PPV, NPV) of 88.8%, 93.8%, 90.9%, and 92.3%, respectively. By including SpO2 as a screening parameter, we achieved superior sensitivity and NPV compared to that reported in our previous paper (sensitivity = 88%;NPV = 82%). Conclusions: Our results suggest that SpO2 is a good screening parameter that improves the accuracy of infection screening. The proposed system has the potential to efficiently identify infected individuals, thereby delaying or preventing the spread of infection during epidemic seasons.

超声引导下竖脊肌平面阻滞对多发肋骨骨折患者术后早期呼吸功能的影响

目的探讨超声引导下竖脊肌平面阻滞(ESPB)对多发肋骨骨折(MRFs)患者术后早期呼吸功能和炎性因子的影响。方法选择2019年2月至2021年12月择期行多发肋骨骨折手术患者58例,男42例,女16例,年龄18~64岁,BMI 18.5~30.0 kg/m^(2),ASAⅠ或Ⅱ级。采用随机数字表法将患者分为两组:ESPB联合全身麻醉组(E组)和单纯全身麻醉组(G组),每组29例。E组于麻醉诱导后在侧卧位下实施超声引导下ESPB,给予0.5%罗哌卡因0.4 ml/kg。记录麻醉诱导前、出PACU时、术后24、48 h的用力肺活量(FVC)、动脉血气分析、静息和咳嗽时VAS疼痛评分。记录术后0~24 h、24~48 h的PCIA有效按压次数和补救镇痛情况。记录麻醉诱导前、术后24、48 h的IL-6和TNF-α浓度。结果与G组比较,E组出PACU时、术后24、48 h的FVC明显增大(P<0.05),PaCO_(2)、静息和咳嗽时VAS疼痛评分明显降低(P<0.05)。与G组比较,E组术后0~24 h、24~48 h的PCIA有效按压次数和补救镇痛率明显降低(P<0.05)。与G组比较,E组术后24、48 h的IL-6和TNF-α浓度明显降低(P<0.05)。结论超声引导下ESPB可为MRFs患者提供良好的术后镇痛,促进术后早期呼吸功能的恢复。

沿海省份城市发展活力评价及其互动关系研究——以副省级城市为例

以德鲁克社会创新为理论基础,基于城市内三大行为活动主体构建包含人居环境、营商环境、治理环境系统的发展活力评价指标体系,利用多因子分层动态激励模型对2006—2020年我国沿海省份8个副省级城市的发展活力进行评价,运用耦合协调模型和面板向量自回归模型分别测度并分析3个子系统间的耦合协调情况和互动关系。结果表明:(1)各城市的发展活力由高到低依次为广州、深圳、杭州、南京、青岛、宁波、大连、厦门,城市间发展活力综合评价结果两极分化明显,营商环境是影响城市发展活力提升最重要的因素,人居环境次之,治理环境最小。(2)8个城市的耦合协调级别不高,整体处于失调类型。(3)3个系统间相互作用,但其发展水平主要受自身影响。其中,人居环境系统显著受其他两个系统影响;营商环境系统受治理环境系统影响较强;治理环境系统主要依靠自身调节,受其他系统的影响较小。

Breeding of Silkworm Variety "Chuancan No.24" with Strong Vitality for Summer-Autumn Rearing

[ Objective] The aim was to introduce the breeding process of natural colorful cocoon silkworm variety, Chuancan No. 24. [ Method] By cross breeding and pedigree separation, the silkworm variety, Chuancan No. 24, with double sex-limited markings and strong vitality and for sum- mer-Autumn rearing was bred. [ Result] The results of laboratory identification and countryside test in Sichuan Province indicated that the Chuancan No. 24 was strong and could be reared easily. They had uniform development and excellent quality of cocoon and silk. In addition, their sexes could be identified by larval body color and markings. The main indexes are as follows： cocoon yield per 10 003 fourth instar larvae, 19.66 kg; filament length, 1 241.29 m; length of non-broken filament, 941.99 mm; reelability, 75.87% ; raw silk ratio of fresh cocoon, 17.07% ; and neatness, 94.03 points. The main economic features of new variety were better than those of the control. [ Conclusion] The Chuancan No. 24 has been approved by the Sichuan Silkworm Evaluation Commission, and it is suitable to rear this new variety in some regions of the Yangtze River drainage basin, such as Sichuan Province and Chongqing.