目的探讨内皮型一氧化氮合酶(NOS3或eNOS)G894T基因多态性与肿瘤易感性的关系。方法通过计算机检索和手工检索,收集有关NOS3G894T基因多态性与肿瘤易感性的文献,筛选出符合纳入和排除标准的文献,应用Meta分析软件对各项研究进行异质性检...目的探讨内皮型一氧化氮合酶(NOS3或eNOS)G894T基因多态性与肿瘤易感性的关系。方法通过计算机检索和手工检索,收集有关NOS3G894T基因多态性与肿瘤易感性的文献,筛选出符合纳入和排除标准的文献,应用Meta分析软件对各项研究进行异质性检验,计算合并OR值及其95%CI,并行敏感性分析和发表偏倚的评估。结果 19篇文献纳入本研究,共计有7 679例肿瘤患者和8 180例对照人群。总体来说,NOS3G894T基因多态性与肿瘤无明显易感性(T versus G:OR=1.030,95%CI0.964-1.101;TT versus GG:OR=1.056,95%CI 0.895-1.245;TG versus GG:OR=1.045,95%CI 0.977-1.119;TT/TG versus GG:OR=1.047,95%CI0.981-1.117;TT versus TG/GG:OR=1.005,95%CI0.900-1.123)。根据种族、肿瘤类型及对照组来源进行分层分析,没有发现有明显易感性。结论 NOS3G894T基因多态性与肿瘤不存在明显易感性。展开更多
目的探讨吲哚美辛抑制非小细胞肺癌的相关机制。方法CCK-8检测NCI-H520、A549肺癌细胞增殖情况;克隆形成实验检测肺癌细胞增殖以及成瘤能力;分子靶向对接预测吲哚美辛相互作用蛋白;GEPIA公共数据库分析肿瘤与癌旁组织、肿瘤切除术后远...目的探讨吲哚美辛抑制非小细胞肺癌的相关机制。方法CCK-8检测NCI-H520、A549肺癌细胞增殖情况;克隆形成实验检测肺癌细胞增殖以及成瘤能力;分子靶向对接预测吲哚美辛相互作用蛋白;GEPIA公共数据库分析肿瘤与癌旁组织、肿瘤切除术后远期生存率与NOS3 mRNA水平关系;免疫组化检测NOS3表达水平;通过LinkedOmics数据库分析NOS3富集的基因;使用KEGG数据库分析与NOS3调控的信号通路;MitoTracker Red CMXRos探针检测线粒体ROS生成水平;电镜下观察线粒体形态;Western blot检测ATP5A、UQCRC2、SDH8、NDUFB8的表达水平。结果20μmol·L^(-1)吲哚美辛可抑制肺癌细胞增殖以及成瘤能力;吲哚美辛与NOS3蛋白存在作用位点;NOS3抑制剂L-NMMA可逆转吲哚美辛抑癌作用。通过生物信息学分析发现,肺癌组织中NOS3表达较低,NOS3基因高表达患者远期生存率更长;NOS3与线粒体呼吸链通路高度负相关。吲哚美辛作用后引起线粒体数量减少、形态肿胀、内脊消失、ROS水平显著上升、显著降低UQCRC2蛋白表达。结论吲哚美辛靶向NOS3抑制UQCRC2蛋白表达从而抑制线粒体呼吸链功能,进而在非小细胞肺癌中发挥抑癌作用。展开更多
Objective: Genetic polymorphisms associated with vascular diseases have been proposed to be involved in the pathogenesis of late unexplained intrauterine fetal death (IUFD). The Nos3 gene is known to regulate vascular...Objective: Genetic polymorphisms associated with vascular diseases have been proposed to be involved in the pathogenesis of late unexplained intrauterine fetal death (IUFD). The Nos3 gene is known to regulate vascular tone via the endothelial nitric oxide synthase/nitric oxide pathway. Study design: In a multicenter case-control study, we evaluated two Nos3 polymorphisms (exon 7 Glu298Asp and a 27 bp-repeat in intron 4) in 92 women with IUFD and 92 healthy control women. Results: The investigated Nos3 polymorphisms were not associated with the occurrence of IUFD. In the subgroup of pregnancies affected by IUFD, women with at least one mutant allele of the Nos3 intron 4 polymorphism were diagnosed with IUFD at a significantly earlier gestational age (31.8 [standard deviation (SD) = 4.9] weeks versus 34.6 [SD=4.8] weeks, p=0.02) and showed a significantly reduced birth weight (2113 g [SD = 1028] versus 1571 g [SD = 568], p = 0.03). Conclusion: We are the first to report on Nos3 polymorphisms and IUFD. While not being associated with the incidence of IUFD overall, the intron 4 Nos3 polymorphism might modulate the timing of IUFD in affected pregnancies.展开更多
目的:探讨转录因子NOS3 SNP rs1799983、rs2070744对宁夏散发性先天性心脏病房间隔缺损(ASD)的影响。方法:采用病例-对照研究方法,运用MassArray SNP技术,对113例散发性先天性心脏病(CHD)患者(病例组)和115例对照组患者进行NOS3 SNP分...目的:探讨转录因子NOS3 SNP rs1799983、rs2070744对宁夏散发性先天性心脏病房间隔缺损(ASD)的影响。方法:采用病例-对照研究方法,运用MassArray SNP技术,对113例散发性先天性心脏病(CHD)患者(病例组)和115例对照组患者进行NOS3 SNP分型检测。结果:①NOS3 SNP rs1799983、rs2070744基因型频率和等位基因频率在CHD组和对照组中的分布差异无统计学意义(P>0.05);②ASD组患者rs1799983 TT与GG基因型在两组中的分布差异有统计学意义,T等位基因与G相比差异有统计学意义(P<0.05)。结论:NOS3 SNP rs1799983在ASD的发病中起作用。展开更多
Cerebral hypoxia or ischemia results in cell death and cerebral edema, as well as other cellular reactions such as angiogenesis and the reestablishment of functional microvasculature to promote recovery from brain inj...Cerebral hypoxia or ischemia results in cell death and cerebral edema, as well as other cellular reactions such as angiogenesis and the reestablishment of functional microvasculature to promote recovery from brain injury. Vascular endothelial growth factor is expressed in the central nervous system after hypoxic/ischemic brain injury, and is involved in the process of brain repair via the regulation of angiogenesis, neurogenesis, neurite outgrowth, and cerebral edema, which all require vascular endothelial growth factor signaling. In this review, we focus on the role of the vascular endothelial growth factor signaling pathway in the response to hypoxic/ischemic brain injury, and discuss potential therapeutic interventions.展开更多
文摘目的探讨内皮型一氧化氮合酶(NOS3或eNOS)G894T基因多态性与肿瘤易感性的关系。方法通过计算机检索和手工检索,收集有关NOS3G894T基因多态性与肿瘤易感性的文献,筛选出符合纳入和排除标准的文献,应用Meta分析软件对各项研究进行异质性检验,计算合并OR值及其95%CI,并行敏感性分析和发表偏倚的评估。结果 19篇文献纳入本研究,共计有7 679例肿瘤患者和8 180例对照人群。总体来说,NOS3G894T基因多态性与肿瘤无明显易感性(T versus G:OR=1.030,95%CI0.964-1.101;TT versus GG:OR=1.056,95%CI 0.895-1.245;TG versus GG:OR=1.045,95%CI 0.977-1.119;TT/TG versus GG:OR=1.047,95%CI0.981-1.117;TT versus TG/GG:OR=1.005,95%CI0.900-1.123)。根据种族、肿瘤类型及对照组来源进行分层分析,没有发现有明显易感性。结论 NOS3G894T基因多态性与肿瘤不存在明显易感性。
文摘目的探讨吲哚美辛抑制非小细胞肺癌的相关机制。方法CCK-8检测NCI-H520、A549肺癌细胞增殖情况;克隆形成实验检测肺癌细胞增殖以及成瘤能力;分子靶向对接预测吲哚美辛相互作用蛋白;GEPIA公共数据库分析肿瘤与癌旁组织、肿瘤切除术后远期生存率与NOS3 mRNA水平关系;免疫组化检测NOS3表达水平;通过LinkedOmics数据库分析NOS3富集的基因;使用KEGG数据库分析与NOS3调控的信号通路;MitoTracker Red CMXRos探针检测线粒体ROS生成水平;电镜下观察线粒体形态;Western blot检测ATP5A、UQCRC2、SDH8、NDUFB8的表达水平。结果20μmol·L^(-1)吲哚美辛可抑制肺癌细胞增殖以及成瘤能力;吲哚美辛与NOS3蛋白存在作用位点;NOS3抑制剂L-NMMA可逆转吲哚美辛抑癌作用。通过生物信息学分析发现,肺癌组织中NOS3表达较低,NOS3基因高表达患者远期生存率更长;NOS3与线粒体呼吸链通路高度负相关。吲哚美辛作用后引起线粒体数量减少、形态肿胀、内脊消失、ROS水平显著上升、显著降低UQCRC2蛋白表达。结论吲哚美辛靶向NOS3抑制UQCRC2蛋白表达从而抑制线粒体呼吸链功能,进而在非小细胞肺癌中发挥抑癌作用。
文摘Objective: Genetic polymorphisms associated with vascular diseases have been proposed to be involved in the pathogenesis of late unexplained intrauterine fetal death (IUFD). The Nos3 gene is known to regulate vascular tone via the endothelial nitric oxide synthase/nitric oxide pathway. Study design: In a multicenter case-control study, we evaluated two Nos3 polymorphisms (exon 7 Glu298Asp and a 27 bp-repeat in intron 4) in 92 women with IUFD and 92 healthy control women. Results: The investigated Nos3 polymorphisms were not associated with the occurrence of IUFD. In the subgroup of pregnancies affected by IUFD, women with at least one mutant allele of the Nos3 intron 4 polymorphism were diagnosed with IUFD at a significantly earlier gestational age (31.8 [standard deviation (SD) = 4.9] weeks versus 34.6 [SD=4.8] weeks, p=0.02) and showed a significantly reduced birth weight (2113 g [SD = 1028] versus 1571 g [SD = 568], p = 0.03). Conclusion: We are the first to report on Nos3 polymorphisms and IUFD. While not being associated with the incidence of IUFD overall, the intron 4 Nos3 polymorphism might modulate the timing of IUFD in affected pregnancies.
文摘目的:探讨转录因子NOS3 SNP rs1799983、rs2070744对宁夏散发性先天性心脏病房间隔缺损(ASD)的影响。方法:采用病例-对照研究方法,运用MassArray SNP技术,对113例散发性先天性心脏病(CHD)患者(病例组)和115例对照组患者进行NOS3 SNP分型检测。结果:①NOS3 SNP rs1799983、rs2070744基因型频率和等位基因频率在CHD组和对照组中的分布差异无统计学意义(P>0.05);②ASD组患者rs1799983 TT与GG基因型在两组中的分布差异有统计学意义,T等位基因与G相比差异有统计学意义(P<0.05)。结论:NOS3 SNP rs1799983在ASD的发病中起作用。
基金supported by the National Natural Science Foundation of China,No.81401238,81330016,31171020,81172174 and 81270724the grants from Ministry of Education of China,No.313037,20110181130002+2 种基金a grant from State Commission of Science Technology of China,No.2012BAI04B04the grants from Science and Technology Bureau of Sichuan Province of China,No.2012SZ0010,2014FZ0113,2014SZ0149a grant from Clinical Discipline Program(Neonatology)from the Ministry of Health of China,No.1311200003303
文摘Cerebral hypoxia or ischemia results in cell death and cerebral edema, as well as other cellular reactions such as angiogenesis and the reestablishment of functional microvasculature to promote recovery from brain injury. Vascular endothelial growth factor is expressed in the central nervous system after hypoxic/ischemic brain injury, and is involved in the process of brain repair via the regulation of angiogenesis, neurogenesis, neurite outgrowth, and cerebral edema, which all require vascular endothelial growth factor signaling. In this review, we focus on the role of the vascular endothelial growth factor signaling pathway in the response to hypoxic/ischemic brain injury, and discuss potential therapeutic interventions.
