UDP-glucose pyrophosphorylase is an important enzyme concerned with carbohydrate metabolism in plants. Cloning of UGPase is a premise for further study on molecular level, and it is also crucial for study of carbohydr...UDP-glucose pyrophosphorylase is an important enzyme concerned with carbohydrate metabolism in plants. Cloning of UGPase is a premise for further study on molecular level, and it is also crucial for study of carbohydrate metabolism. UGPase cDNA sequence as a template, designed primer, then 3＇-untranslate region （3＇ UTR） of UGPase were amplified by 3＇-rapid amplification of cDNA ends （3＇-RACE）. The results suggested the 3＇ UTR were 243 bp, contained AATAA sequence and Poly（A）.展开更多
An isolate of PVS,HZ00P1,was obtained from nature infected Solanum tuberosum from Hangzhou,Zhejiang province.It was primarily identified by DAS-ELISA and host reaction tests.3’ end partial sequence of the virus isola...An isolate of PVS,HZ00P1,was obtained from nature infected Solanum tuberosum from Hangzhou,Zhejiang province.It was primarily identified by DAS-ELISA and host reaction tests.3’ end partial sequence of the virus isolate was cloned.Nucleic acid sequence of the cp gene and deduced cp amino acid sequence alignments were compared with 16 other isolates registered in GenBank.The results showed that the 17 PVS isolates were divided into two groups.Among them,two Andean isolates were classified to groupⅡ,HZ00P1 and the other 14 isolates were assigned to groupⅠ.Compared with other PVS isolates in group Ⅰ,PVS HZ00P1 had 93.1%-98.1% and 95.9%-99.3% homologies of nucleotide sequence and deduced amino acid sequence respectively,whereas its similarities to the groupⅡwere 81.4%-81.7% and 93.5%-93.9%.A survey of PVS occurrence in Zhejiang province was achieved by RNA spot hybridization(RSH).Among the 30 samples collected from different areas of the province,26.7% were found to have the infection of PVS.It is concluded that PVS has become a common virus in Zhejiang province.展开更多
文摘UDP-glucose pyrophosphorylase is an important enzyme concerned with carbohydrate metabolism in plants. Cloning of UGPase is a premise for further study on molecular level, and it is also crucial for study of carbohydrate metabolism. UGPase cDNA sequence as a template, designed primer, then 3＇-untranslate region （3＇ UTR） of UGPase were amplified by 3＇-rapid amplification of cDNA ends （3＇-RACE）. The results suggested the 3＇ UTR were 243 bp, contained AATAA sequence and Poly（A）.
文摘An isolate of PVS,HZ00P1,was obtained from nature infected Solanum tuberosum from Hangzhou,Zhejiang province.It was primarily identified by DAS-ELISA and host reaction tests.3’ end partial sequence of the virus isolate was cloned.Nucleic acid sequence of the cp gene and deduced cp amino acid sequence alignments were compared with 16 other isolates registered in GenBank.The results showed that the 17 PVS isolates were divided into two groups.Among them,two Andean isolates were classified to groupⅡ,HZ00P1 and the other 14 isolates were assigned to groupⅠ.Compared with other PVS isolates in group Ⅰ,PVS HZ00P1 had 93.1%-98.1% and 95.9%-99.3% homologies of nucleotide sequence and deduced amino acid sequence respectively,whereas its similarities to the groupⅡwere 81.4%-81.7% and 93.5%-93.9%.A survey of PVS occurrence in Zhejiang province was achieved by RNA spot hybridization(RSH).Among the 30 samples collected from different areas of the province,26.7% were found to have the infection of PVS.It is concluded that PVS has become a common virus in Zhejiang province.
