ADAMTS-1(a d isintegrin and m etalloprotease w ith thrombospond in type 1 motifs)是一种新发现的金属蛋白酶,来源于肿瘤细胞,因其蛋白质分子-C末端的特殊结构可以结合细胞外基质,通过介导细胞与细胞以及细胞与细胞外基质的相互作...ADAMTS-1(a d isintegrin and m etalloprotease w ith thrombospond in type 1 motifs)是一种新发现的金属蛋白酶,来源于肿瘤细胞,因其蛋白质分子-C末端的特殊结构可以结合细胞外基质,通过介导细胞与细胞以及细胞与细胞外基质的相互作用,从而在个体生长发育、泌尿生殖器官形态改变以及炎症、肿瘤生长转移、动脉粥样硬化等生理和病理过程中发挥重要作用。展开更多
目的检测ADAMTS-(a disintegrin and metalloprotease with throm- spondin type 1 motifs)在急、慢性柯萨奇病毒B3(CVB3)心肌炎小鼠心肌组织中的表达,并分析其与心肌纤维化的关系。方法以CVB3单次感染或重复增量感染Ba1b／C小鼠分别建...目的检测ADAMTS-(a disintegrin and metalloprotease with throm- spondin type 1 motifs)在急、慢性柯萨奇病毒B3(CVB3)心肌炎小鼠心肌组织中的表达,并分析其与心肌纤维化的关系。方法以CVB3单次感染或重复增量感染Ba1b／C小鼠分别建立急性病毒性心肌炎(急性组,20只)及慢性病毒性心肌炎(慢性组,25只)模型,同期小鼠腹腔无菌注射等剂量不含病毒的EMEM液作为各自正常对照组(均为10只)。以苦味酸天狼猩红行心肌胶原组织特异性染色,并运用图像分析软件计算心肌组织中的胶原容积积分(collagen volume fraction,CVF,％;用反转录-聚合酶链反应(RT-PCR)和免疫组织化学方法分别检测小鼠心肌组织中ADAMTS-1基因水平和蛋白水平的表达。结果两组病毒性心肌炎小鼠心肌组织中CVF显著增加,且慢性组更为显著(P<0．01):急、慢性组ADAMTS-1 mRNA均明显高于其正常对照组,尤以慢性组升高更为显著(P<0．05);免疫组织化学检测结果显示, ADAMTS-1在心肌组织细胞胞质内表达;各实验组小鼠心肌组织中ADAMTS-1 mRNA的表达与CVF呈正相关。结论ADAMTS-1在急、慢性病毒性心肌炎心肌组织中呈进行性增加并与胶原增生密切相关,表明ADAMTS-1可能通过调节胶原代谢参与心肌纤维化的发生、发展。展开更多
ADAMTS-1是一个新发现的金属蛋白酶ADAMTS(adisintegrin and metalloprotease with thrombospondin type1motif)家族的一员。排卵时ADAMTS-1在卵巢颗粒细胞层的转录活性明显增强,并且其转录活性的增强依赖于黄体生成素(luteinizing horm...ADAMTS-1是一个新发现的金属蛋白酶ADAMTS(adisintegrin and metalloprotease with thrombospondin type1motif)家族的一员。排卵时ADAMTS-1在卵巢颗粒细胞层的转录活性明显增强,并且其转录活性的增强依赖于黄体生成素(luteinizing hormone,LH)峰值的出现和孕激素受体(progesterone receptor,PR)的表达,孕激素受体基因敲除小鼠(PRKO小鼠)卵巢局部ADAMTS-1表达显著下降,有明显的排卵障碍,提示:ADAMTS-1是PR的直接下游靶基因,可能是LH和P(PR)作用下诱导排卵的关键蛋白水解酶。展开更多
目的研究大鼠蛛网膜下腔出血(subarachnoid hemorrhage,SAH)后早期海马组织中ADAMTS-l(a disintegrin-like and metalloproteinase with thrombospondin type l motifs)表达情况及其与血脑屏障通透性的关系,初步探讨ADAMTS-l在实验性大...目的研究大鼠蛛网膜下腔出血(subarachnoid hemorrhage,SAH)后早期海马组织中ADAMTS-l(a disintegrin-like and metalloproteinase with thrombospondin type l motifs)表达情况及其与血脑屏障通透性的关系,初步探讨ADAMTS-l在实验性大鼠SAH后早期脑损伤(early brain injury,EBI)中的作用。方法成年雄性SD大鼠108只,分假手术组和SAH组。采用大鼠视交叉前池注血模型,于SAH后6、12、24、48、72 h时间点,应用Western-Blotting和RT-PCR法检测大鼠海马ADAMTS-1蛋白及mRNA表达变化,同时应用甲酰胺浸泡法检测大鼠脑内伊文氏蓝(Evans blue,EB)含量。结果大鼠SAH后6 h、12 h组海马ADAMTS-1mRNA及蛋白表达与sham组相比无统计学意义,于出血后24 h表达明显升高,48 h达到高峰,72 h后仍维持在较高水平。脑内伊文氏蓝含量亦于出血后24 h明显升高,48 h达到高峰并于72 h仍维持在较高水平。ADAMTS-1蛋白表达与脑内EB含量成正相关(r=0.936,P=0.014)。结论大鼠SAH后早期脑损伤过程中ADAMTS-l的表达与血脑屏障损伤成正相关,提示ADAMTS-l可能参与SAH后早期脑损伤的病理过程。展开更多
The nucleotide sequences in exon 7 of a disintegrin and metalloproteinase with thrombospondin motifs-1(ADAMTS-1)gene in Landrace pig were obtained with cloning and sequencing by using PCR-RFLP technique in this experi...The nucleotide sequences in exon 7 of a disintegrin and metalloproteinase with thrombospondin motifs-1(ADAMTS-1)gene in Landrace pig were obtained with cloning and sequencing by using PCR-RFLP technique in this experiment.Sequence alignment results showed that the C/G substitution in exon 7 induced amino acid change(Arg → Pro).AA,BB and AB genotypes were found in Landrace pig by PvuⅡ-RFLP.Correlation analysis between genotypes and reproductive traits indicated that ADAMTS-1 was significantly correlated with litter size(LS)and number born alive(NBA).展开更多
Objective: To investigate the correlation of serum ADAMTS-1 and ADAMTS-13 contents with plaque nature, inflammatory factors and adipocytokines in patients with coronary heart disease. Methods: A total of 106 patients ...Objective: To investigate the correlation of serum ADAMTS-1 and ADAMTS-13 contents with plaque nature, inflammatory factors and adipocytokines in patients with coronary heart disease. Methods: A total of 106 patients with coronary heart disease undergoing examination in the hospital between September 2014 and June 2017 were enrolled in coronary heart disease group, and 100 healthy volunteers were enrolled in normal control group. Differences in serum ADAMTS-1, ADAMTS-13, plaque nature-related index, inflammatory factor and adipocytokine contents were compared between the two groups. Pearson test was used to assess the correlation of ADAMTS-1 and ADAMTS-13 contents with plaque nature, inflammatory factor and adipocytokines in patients with coronary heart disease. Results: Serum ADAMTS-1 content of coronary heart disease group was higher than that of normal control group whereas ADAMTS-13 content was lower than that of normal control group;serum plaque nature-related indexes Hcy and UA contents were higher than those of normal control group;serum inflammatory factors HMGB1, IL-6, IL-8 and IL-18 contents were higher than those of normal control group;serum adipocytokines APN and Vaspin contents were lower than those of normal control group whereas Visfatin and RBP4 contents were higher than those of normal control group. Pearson test showed that serum ADAMTS-1 and ADAMTS-13 contents of patients with coronary heart disease were directly correlated with the contents of plaque nature-related indexes, inflammatory factors and adipocytokines. Conclusion: Serum ADAMTS-1 content abnormally increases and ADAMTS-13 content abnormally decreases in patients with coronary heart disease, and the specific contents are directly correlated with the condition of coronary heart disease.展开更多
文摘ADAMTS-1(a d isintegrin and m etalloprotease w ith thrombospond in type 1 motifs)是一种新发现的金属蛋白酶,来源于肿瘤细胞,因其蛋白质分子-C末端的特殊结构可以结合细胞外基质,通过介导细胞与细胞以及细胞与细胞外基质的相互作用,从而在个体生长发育、泌尿生殖器官形态改变以及炎症、肿瘤生长转移、动脉粥样硬化等生理和病理过程中发挥重要作用。
文摘目的检测ADAMTS-(a disintegrin and metalloprotease with throm- spondin type 1 motifs)在急、慢性柯萨奇病毒B3(CVB3)心肌炎小鼠心肌组织中的表达,并分析其与心肌纤维化的关系。方法以CVB3单次感染或重复增量感染Ba1b／C小鼠分别建立急性病毒性心肌炎(急性组,20只)及慢性病毒性心肌炎(慢性组,25只)模型,同期小鼠腹腔无菌注射等剂量不含病毒的EMEM液作为各自正常对照组(均为10只)。以苦味酸天狼猩红行心肌胶原组织特异性染色,并运用图像分析软件计算心肌组织中的胶原容积积分(collagen volume fraction,CVF,％;用反转录-聚合酶链反应(RT-PCR)和免疫组织化学方法分别检测小鼠心肌组织中ADAMTS-1基因水平和蛋白水平的表达。结果两组病毒性心肌炎小鼠心肌组织中CVF显著增加,且慢性组更为显著(P<0．01):急、慢性组ADAMTS-1 mRNA均明显高于其正常对照组,尤以慢性组升高更为显著(P<0．05);免疫组织化学检测结果显示, ADAMTS-1在心肌组织细胞胞质内表达;各实验组小鼠心肌组织中ADAMTS-1 mRNA的表达与CVF呈正相关。结论ADAMTS-1在急、慢性病毒性心肌炎心肌组织中呈进行性增加并与胶原增生密切相关,表明ADAMTS-1可能通过调节胶原代谢参与心肌纤维化的发生、发展。
文摘ADAMTS-1是一个新发现的金属蛋白酶ADAMTS(adisintegrin and metalloprotease with thrombospondin type1motif)家族的一员。排卵时ADAMTS-1在卵巢颗粒细胞层的转录活性明显增强,并且其转录活性的增强依赖于黄体生成素(luteinizing hormone,LH)峰值的出现和孕激素受体(progesterone receptor,PR)的表达,孕激素受体基因敲除小鼠(PRKO小鼠)卵巢局部ADAMTS-1表达显著下降,有明显的排卵障碍,提示:ADAMTS-1是PR的直接下游靶基因,可能是LH和P(PR)作用下诱导排卵的关键蛋白水解酶。
文摘The nucleotide sequences in exon 7 of a disintegrin and metalloproteinase with thrombospondin motifs-1(ADAMTS-1)gene in Landrace pig were obtained with cloning and sequencing by using PCR-RFLP technique in this experiment.Sequence alignment results showed that the C/G substitution in exon 7 induced amino acid change(Arg → Pro).AA,BB and AB genotypes were found in Landrace pig by PvuⅡ-RFLP.Correlation analysis between genotypes and reproductive traits indicated that ADAMTS-1 was significantly correlated with litter size(LS)and number born alive(NBA).
文摘Objective: To investigate the correlation of serum ADAMTS-1 and ADAMTS-13 contents with plaque nature, inflammatory factors and adipocytokines in patients with coronary heart disease. Methods: A total of 106 patients with coronary heart disease undergoing examination in the hospital between September 2014 and June 2017 were enrolled in coronary heart disease group, and 100 healthy volunteers were enrolled in normal control group. Differences in serum ADAMTS-1, ADAMTS-13, plaque nature-related index, inflammatory factor and adipocytokine contents were compared between the two groups. Pearson test was used to assess the correlation of ADAMTS-1 and ADAMTS-13 contents with plaque nature, inflammatory factor and adipocytokines in patients with coronary heart disease. Results: Serum ADAMTS-1 content of coronary heart disease group was higher than that of normal control group whereas ADAMTS-13 content was lower than that of normal control group;serum plaque nature-related indexes Hcy and UA contents were higher than those of normal control group;serum inflammatory factors HMGB1, IL-6, IL-8 and IL-18 contents were higher than those of normal control group;serum adipocytokines APN and Vaspin contents were lower than those of normal control group whereas Visfatin and RBP4 contents were higher than those of normal control group. Pearson test showed that serum ADAMTS-1 and ADAMTS-13 contents of patients with coronary heart disease were directly correlated with the contents of plaque nature-related indexes, inflammatory factors and adipocytokines. Conclusion: Serum ADAMTS-1 content abnormally increases and ADAMTS-13 content abnormally decreases in patients with coronary heart disease, and the specific contents are directly correlated with the condition of coronary heart disease.
