Background Chinese indigenous pigs are popular with consumers for their juiciness,flavour and meat quality,but they have lower meat production.Insulin-like growth factor 2(IGF2) is a maternally imprinted growth factor...Background Chinese indigenous pigs are popular with consumers for their juiciness,flavour and meat quality,but they have lower meat production.Insulin-like growth factor 2(IGF2) is a maternally imprinted growth factor that promotes skeletal muscle growth by regulating cell proliferation and differentiation.A single nucleotide polymorphism(SNP) within intron 3 of porcine IGF2 disrupts a binding site for the repressor,zinc finger BED-type containing 6(ZBED6),leading to up-regulation of IGF2 and causing major effects on muscle growth,heart size,and backfat thickness.This favorable mutation is common in Western commercial pig populations,but absent in most Chinese indigenous pig breeds.To improve meat production of Chinese indigenous pigs,we used cytosine base editor 3(CBE3)to introduce IGF2 intron3-C3071T mutation into porcine embryonic fibroblasts(PEFs) isolated from a male Liang Guang Small Spotted pig(LGSS),and single-cell clones harboring the desired mutation were selected for somatic cell nuclear transfer(SCNT) to generate the founder line of IGF2^(T/T) pigs.Results We found the heterozygous progeny IGF2^(C/T) pigs exhibited enhanced expression of IGF2,increased lean meat by 18%-36%,enlarged loin muscle area by 3%-17%,improved intramuscular fat(IMF) content by 18%-39%,marbling score by 0.75-1,meat color score by 0.53-1.25,and reduced backfat thickness by 5%-16%.The enhanced accumulation of intramuscular fat in IGF2^(C/T) pigs was identified to be regulated by the PI3K-AKT/AMPK pathway,which activated SREBP1 to promote adipogenesis.Conclusions We demonstrated the introduction of IGF2-intron3-C3071T in Chinese LGSS can improve both meat production and quality,and first identified the regulation of IMF deposition by IGF2 through SREBP1 via the PI3KAKT/AMPK signaling pathways.Our study provides a further understanding of the biological functions of IGF2and an example for improving porcine economic traits through precise base editing.展开更多
当前,产教融合进入新发展阶段,为满足其呈现的新特征与新要求。本研究旨在构建新型教学模式,以旅游营销策划课程为例,将CBE与CDIO模式相结合,采用分段式教学。在前段教学过程中,采用CBE模式进行模块化教学,利用DACUM职业能力分析法,深...当前,产教融合进入新发展阶段,为满足其呈现的新特征与新要求。本研究旨在构建新型教学模式,以旅游营销策划课程为例,将CBE与CDIO模式相结合,采用分段式教学。在前段教学过程中,采用CBE模式进行模块化教学,利用DACUM职业能力分析法,深入剖析学生未来工作中所需能力,并以此与课程模块相对应,明确学习目标。在后段教学过程中,采用CDIO模式,以项目为教学载体,组建学生小组,协同完成项目任务,通过四阶段考察学生的知识迁移能力。本研究通过CBE-CDIO模式,旨在培养旅游管理专业学生的通用能力,如沟通能力、灵活应变能力和自我管理能力,以及专项能力,如团队合作能力和高阶思维能力。这一新思路以期为高等教育提供新思路与新方法,实现培养高素质应用型人才的目标。Currently, the integration of industry and education has entered a new development stage, in order to meet the new features and requirements it presents. This study aims to construct a new teaching mode, combining CBE and CDIO modes, and adopting segmented teaching. In the first part of the teaching process, the CBE model is used for modular teaching, and the DACUM vocational competence analysis method is used to deeply analyse the competences required by students in their future work, and correspond to the course modules, so as to clarify the learning objectives. In the latter part of the teaching process, the CDIO model is adopted, using the project as the teaching carrier, forming student groups to collaboratively complete project tasks, and examining students’ knowledge transfer ability through four stages. This study aims to develop students’ generic competencies, such as communication, flexibility and self-management, and specialised competencies, such as teamwork and higher-order thinking, through the CBE-CDIO model. This new idea aims to provide new ideas and methods for higher education to achieve the goal of cultivating high-quality applied talents.展开更多
随着市场需求的不断变化,商业空间景观设计课程建设要求越来越高。以商业空间景观设计课程为例,在分析CBE(Competency Based Education)教学模式理论内涵和实践特点的基础上,分析了商业空间景观设计课程的现状,探讨了CBE教学模式在商业...随着市场需求的不断变化,商业空间景观设计课程建设要求越来越高。以商业空间景观设计课程为例,在分析CBE(Competency Based Education)教学模式理论内涵和实践特点的基础上,分析了商业空间景观设计课程的现状,探讨了CBE教学模式在商业空间景观设计课程中的应用,并提出课程发展建议。以期为其他民办高校的教学改革提供新思路,培养出更多符合社会需求和市场需要的专业人才。展开更多
基金supported by the National Natural Science Foundation of China (3207269732030102)+2 种基金CARS-PIG-35R&D Programmes of Guangdong Province (2018B020203003)Laboratory of Lingnan Modern Agriculture Project (NZ2021006)。
文摘Background Chinese indigenous pigs are popular with consumers for their juiciness,flavour and meat quality,but they have lower meat production.Insulin-like growth factor 2(IGF2) is a maternally imprinted growth factor that promotes skeletal muscle growth by regulating cell proliferation and differentiation.A single nucleotide polymorphism(SNP) within intron 3 of porcine IGF2 disrupts a binding site for the repressor,zinc finger BED-type containing 6(ZBED6),leading to up-regulation of IGF2 and causing major effects on muscle growth,heart size,and backfat thickness.This favorable mutation is common in Western commercial pig populations,but absent in most Chinese indigenous pig breeds.To improve meat production of Chinese indigenous pigs,we used cytosine base editor 3(CBE3)to introduce IGF2 intron3-C3071T mutation into porcine embryonic fibroblasts(PEFs) isolated from a male Liang Guang Small Spotted pig(LGSS),and single-cell clones harboring the desired mutation were selected for somatic cell nuclear transfer(SCNT) to generate the founder line of IGF2^(T/T) pigs.Results We found the heterozygous progeny IGF2^(C/T) pigs exhibited enhanced expression of IGF2,increased lean meat by 18%-36%,enlarged loin muscle area by 3%-17%,improved intramuscular fat(IMF) content by 18%-39%,marbling score by 0.75-1,meat color score by 0.53-1.25,and reduced backfat thickness by 5%-16%.The enhanced accumulation of intramuscular fat in IGF2^(C/T) pigs was identified to be regulated by the PI3K-AKT/AMPK pathway,which activated SREBP1 to promote adipogenesis.Conclusions We demonstrated the introduction of IGF2-intron3-C3071T in Chinese LGSS can improve both meat production and quality,and first identified the regulation of IMF deposition by IGF2 through SREBP1 via the PI3KAKT/AMPK signaling pathways.Our study provides a further understanding of the biological functions of IGF2and an example for improving porcine economic traits through precise base editing.
文摘当前,产教融合进入新发展阶段,为满足其呈现的新特征与新要求。本研究旨在构建新型教学模式,以旅游营销策划课程为例,将CBE与CDIO模式相结合,采用分段式教学。在前段教学过程中,采用CBE模式进行模块化教学,利用DACUM职业能力分析法,深入剖析学生未来工作中所需能力,并以此与课程模块相对应,明确学习目标。在后段教学过程中,采用CDIO模式,以项目为教学载体,组建学生小组,协同完成项目任务,通过四阶段考察学生的知识迁移能力。本研究通过CBE-CDIO模式,旨在培养旅游管理专业学生的通用能力,如沟通能力、灵活应变能力和自我管理能力,以及专项能力,如团队合作能力和高阶思维能力。这一新思路以期为高等教育提供新思路与新方法,实现培养高素质应用型人才的目标。Currently, the integration of industry and education has entered a new development stage, in order to meet the new features and requirements it presents. This study aims to construct a new teaching mode, combining CBE and CDIO modes, and adopting segmented teaching. In the first part of the teaching process, the CBE model is used for modular teaching, and the DACUM vocational competence analysis method is used to deeply analyse the competences required by students in their future work, and correspond to the course modules, so as to clarify the learning objectives. In the latter part of the teaching process, the CDIO model is adopted, using the project as the teaching carrier, forming student groups to collaboratively complete project tasks, and examining students’ knowledge transfer ability through four stages. This study aims to develop students’ generic competencies, such as communication, flexibility and self-management, and specialised competencies, such as teamwork and higher-order thinking, through the CBE-CDIO model. This new idea aims to provide new ideas and methods for higher education to achieve the goal of cultivating high-quality applied talents.
文摘随着市场需求的不断变化,商业空间景观设计课程建设要求越来越高。以商业空间景观设计课程为例,在分析CBE(Competency Based Education)教学模式理论内涵和实践特点的基础上,分析了商业空间景观设计课程的现状,探讨了CBE教学模式在商业空间景观设计课程中的应用,并提出课程发展建议。以期为其他民办高校的教学改革提供新思路,培养出更多符合社会需求和市场需要的专业人才。
