Highly purified CD71 protein was isolated from human placental trophoblasts and Molt-4 cells with affinity chromatography and was shown with SDS-PAGE as an obvious protein band of 90 ×103u under reducing conditio...Highly purified CD71 protein was isolated from human placental trophoblasts and Molt-4 cells with affinity chromatography and was shown with SDS-PAGE as an obvious protein band of 90 ×103u under reducing condition and about 180 × 103u under non-reducing condition. The protein molecules were reconstituted into artificial membranes composed of different ratios of phospholipids (PL)/cholesterol (Ch). Circular dichroism analysis demonstrated that there was a decrease of the beta-laminar structure and an increase of alpha-helica and random coils when the Ch content of the artificial membranes DMPC/Ch or DOPC/Ch was increased. Tryptophan fluorescence spectrum also demonstrated that along with the changes of the lipid constituents of the artificial membrane,the spatial conformation of CD71 protein underwent corresponding changes.展开更多
文摘Highly purified CD71 protein was isolated from human placental trophoblasts and Molt-4 cells with affinity chromatography and was shown with SDS-PAGE as an obvious protein band of 90 ×103u under reducing condition and about 180 × 103u under non-reducing condition. The protein molecules were reconstituted into artificial membranes composed of different ratios of phospholipids (PL)/cholesterol (Ch). Circular dichroism analysis demonstrated that there was a decrease of the beta-laminar structure and an increase of alpha-helica and random coils when the Ch content of the artificial membranes DMPC/Ch or DOPC/Ch was increased. Tryptophan fluorescence spectrum also demonstrated that along with the changes of the lipid constituents of the artificial membrane,the spatial conformation of CD71 protein underwent corresponding changes.
