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Coumarin and eugenol ameliorate LPS-induced inflammation in RAW 264.7 cells via modulating the NLRP3 inflammasome pathway
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作者 Jyotsana Bakshi Somnath Singh KP Mishra 《Asian Pacific Journal of Tropical Biomedicine》 SCIE CAS 2024年第1期40-46,共7页
Objective:To investigate the underlying mechanism of anti-inflammatory action of coumarin and eugenol in lipopolysaccharide(LPS)-stimulated RAW 264.7 cells.Methods:RAW 264.7 cells were treated with 2.5μg/mL of LPS,50... Objective:To investigate the underlying mechanism of anti-inflammatory action of coumarin and eugenol in lipopolysaccharide(LPS)-stimulated RAW 264.7 cells.Methods:RAW 264.7 cells were treated with 2.5μg/mL of LPS,50μM of coumarin,and 50μM eugenol for 24 h.The viability of the cells was assessed using MTT assay.The production of nitric oxide was determined using Griess reagent and DCFH-DA was used to measure the production of reactive oxygen species.The protein expression of NLRP3,IL-1β,NF-κB,and cyclooxygenase 2 was assessed using Western blot analysis.Results:Coumarin and eugenol showed anti-inflammatory effects against LPS-induced inflammatory response by ameliorating the expression of NLRP3 inflammasome and NF-κB,which further led to a subsequent reduction in IL-1β,nitric oxide,and reactive oxygen species.Conclusions:Coumarin and eugenol exert their anti-inflammatory activities by modulating the NLRP3 inflammasome pathway and NF-κB.These compounds may have promising therapeutic applications for the treatment of various inflammatory diseases. 展开更多
关键词 INFLAMMATION CYTOKINES COUMARIN eugenol NLRP3 LPS
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Eugenol targeting CrtM inhibits the biosynthesis of staphyloxanthin in Staphylococcus aureus
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作者 Jiang Chang Bo Chen +7 位作者 Zeqian Du Bowen Zhao Jiahui Li Ziyi Li Kannappan Arunachalam Ting Shi Dongqing Wei Chunlei Shi 《Food Science and Human Wellness》 SCIE CSCD 2024年第3期1368-1377,共10页
Staphylococcus aureus is a serious foodborne pathogen threatening food safety and public health.Especially the emergence of methicillin-resistant Staphylococcus aureus(MRSA)increased the difficulty of S.aureus treatme... Staphylococcus aureus is a serious foodborne pathogen threatening food safety and public health.Especially the emergence of methicillin-resistant Staphylococcus aureus(MRSA)increased the difficulty of S.aureus treatment.Staphyloxanthin is a crucial virulence factor of S.aureus.Blocking staphyloxanthin production could help the host immune system counteract the invading S.aureus cells.In this study,we first screened for staphyloxanthin inhibitors using a virtual screening method.The outcome of the virtual screening method resulted in the identification of eugenol(300μg/mL),which significantly inhibits the staphyloxanthin production in S.aureus ATCC 29213,S.aureus Newman,MRSA ATCC 43300 and MRSA ATCC BAA1717by 84.2%,63.5%,68.1%,and 79.5%,respectively.The outcome of the growth curve assay,field-emission scanning electron,and confocal laser scanning microscopy analyses confirmed that eugenol at the test concentration did not affect the morphology and growth of S.aureus.Moreover,the survival rate of S.aureus ATCC 29213 and MRSA ATCC 43300 under H_(2)O_(2) pressure decreased to 51.9%and 45.5%in the presence of eugenol,respectively.The quantitative RT-PCR and molecular simulation studies revealed that eugenol targets staphyloxanthin biosynthesis by downregulating the transcription of the crtM gene and inhibiting the activity of the CrtM enzyme.Taken together,we first determined that eugenol was a prominent compound for staphyloxanthin inhibitor to combat S.aureus especially MRSA infections. 展开更多
关键词 Staphylococcus aureus Staphyloxanthin eugenol Virtual screening 4 4’-Diapophytoene synthase(CrtM)
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Enhancement of the Antigenotoxic and Antioxidant Actions of Eugenol from Spice Clove and the Stabilizer Gum Arabic on Colorectal Carcinogenesis
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作者 Nayanna de Oliveira Ramos Melo Lucas Gabriel da Costa Marques +5 位作者 Humberto Maia Costa Neto Matheus De Sousa Silva Francisco Vagnaldo Fechine Jamacaru Bruno Coêlho Cavalcanti Antônio Adailson De Sousa Silva Conceição Aparecida Dornelas 《Food and Nutrition Sciences》 CAS 2024年第1期71-100,共30页
Spices are defined as any aromatic condiment of plant origin used to alter the flavor and aroma of foods. Besides flavor and aroma, many spices have antioxidant activity, mainly related to the presence in cloves of ph... Spices are defined as any aromatic condiment of plant origin used to alter the flavor and aroma of foods. Besides flavor and aroma, many spices have antioxidant activity, mainly related to the presence in cloves of phenolic compounds, such as flavonoids, terpenoids and eugenol. In turn, the most common uses of gum arabic are in the form of powder for addition to soft drink syrups, cuisine and baked goods, specifically to stabilize the texture of products, increase the viscosity of liquids and promote the leavening of baked products (e.g., cakes). Both eugenol, extracted from cloves, and gum arabic, extracted from the hardened sap of two species of the Acacia tree, are dietary constituents routinely consumed virtually throughout the world. Both of them are also widely used medicinally to inhibit oxidative stress and genotoxicity. The prevention arm of the study included groups: Ia, IIa, IIIa, Iva, V, VI, VII, VIII. Once a week for 20 weeks, the controls received saline s.c. while the experimental groups received DMH at 20 mg/kg s.c. During the same period and for an additional 9 weeks, the animals received either water, 10% GA, EUG, or 10% GA + EUG by gavage. The treatment arm of the study included groups Ib, IIb, IIIb e IVb, IX, X, XI, XII). Once a week for 20 weeks, the controls received saline s.c. while the experimental groups received DMH at 20 mg/kg s.c. During the subsequent 9 weeks, the animals received either water, 10% GA, EUG or 10% GA + EUG by gavage. The novelty of this study is the investigation of their use alone and together for the prevention and treatment of experimental colorectal carcinogenesis induced by dimethylhydrazine. Our results show that the combined use of 10% gum arabic and eugenol was effective, with antioxidant action in the colon, as well as reducing oxidative stress in all colon segments and preventing and treating genotoxicity in all colon segments. Furthermore, their joint administration reduced the number of aberrant crypts and the number of aberrant crypt foci (ACF) in the distal segment and entire colon, as well as the number of ACF with at least 5 crypts in the entire colon. Thus, our results also demonstrate the synergistic effects of 10% gum arabic together with eugenol (from cloves), with antioxidant, antigenotoxic and anticarcinogenic actions (prevention and treatment) at the doses and durations studied, in the colon of rats submitted to colorectal carcinogenesis induced by dimethylhydrazine. 展开更多
关键词 eugenol Gum Arabic CARCINOGENESIS Oxidative Stress GENOTOXICITY
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Studies on Anesthetic Effect of Eugenol on Juvenile Fenneropenaeus chinensis 被引量:2
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作者 黄雪芹 孔杰 +2 位作者 张天时 罗坤 赖光艳 《Agricultural Science & Technology》 CAS 2008年第3期115-118,共4页
[Objective] The aim of the study is to seek a good anesthetic to Fenneropenaeus chinensis. [Method] The anesthetic effect of eugenol to juvenile Fenneropenaeus chinensis was investigated. [Result] The juveniles could ... [Objective] The aim of the study is to seek a good anesthetic to Fenneropenaeus chinensis. [Method] The anesthetic effect of eugenol to juvenile Fenneropenaeus chinensis was investigated. [Result] The juveniles could be effectively anaesthetized by 50-400 mg/L eugenol aqueous solution with temperature of 24 ℃. Within the concentration range of 50-400 mg/L, the increase of the eugenol concentration could shorten the time required for anesthesia, meanwhile could prolong the time for recovery. The recovered rate of prawn reached 100% when the eugenol concentration was lower than 200 mg/L, while the recovered rate of prawn was just 66.67% when the eugenol concentration was higher than 400 mg/L. The survival rate of prawns in test group was 100% from the observation of three consecutive days. For the specific dose, the anesthetic effect enhanced with the increase of water temperature (18-27℃). [Conclusion] Eugenol is a safe and efficient anesthetics that can be applied in genetic breeding of prawn. 展开更多
关键词 Fenneropenaeus CHINENSIS eugenol ANESTHETIC effect
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Cold Stress-induced Glucosyltransferase CsUGT78A15 is Involved in the Formation of Eugenol Glucoside in Camellia sinensis 被引量:7
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作者 Mingyue Zhao Binbin Cai +12 位作者 Jieyang Jin Na Zhang Tingting Jing Jingming Wang Yuting Pan Zixiang Zhou Yifan Zhao Yingying Feng Feng Yu Mengting Zhang Yating Li Zhonghua Liu Chuankui Song 《Horticultural Plant Journal》 SCIE 2020年第6期439-449,共11页
Eugenol is a natural phenolic compound known for its health-promoting properties and its ability to add a floral scent to tea plants.Plant eugenol glycosides have been identified and shown to make important contributi... Eugenol is a natural phenolic compound known for its health-promoting properties and its ability to add a floral scent to tea plants.Plant eugenol glycosides have been identified and shown to make important contributions to fruit floral quality.However,the details of their biosynthesis and metabolism in tea plants are still unknown.Here,eugenol glucoside was unambiguously identified as a native metabolite in the tea plant,and its biosynthesis was shown to be induced by low temperature treatment.Through the analysis of UGTs induced by low temperature,the glycosyltransferase CsUGT78A15 was identified in tea,and its encoded protein was shown to catalyze the glucosylation of eugenol.Vmax/Km ratios showed that eugenol was the most suitable substrate for CsUGT78A15.Sugar donor preference analysis showed that CsUGT78A15 had a higher selectivity for glucose,followed by galactose and glucuronic acid.The expression of CsUGT78A15was correlatedwith the accumulation of eugenol glucoside in different tissues and genotypes of tea.Down-regulation of CsUGT78A15 led to a decreased eugenol glucoside content under cold stress,indicating that CsUGT78A15 plays an important role in the biosynthesis of eugenol glucoside under cold stress.The identification of eugenol glucoside in the tea plant and the discovery of a cold stress-induced eugenol glucosyltransferase in tea provide the foundation for the improvement of tea flavor under cold stress and the biotechnological production of eugenol glucoside. 展开更多
关键词 Camellia sinensis eugenol GLUCOSYLTRANSFERASE Cold stress
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MgFe hydrotalcites-derived layered structure iron molybdenum sulfide catalysts for eugenol hydrodeoxygenation to produce phenolic chemicals
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作者 Xinyong Diao Na Ji +7 位作者 Mingyuan Zheng Qingling Liu Chunfeng Song Yibo Huang Qing Zhang Alazar Alemayehu Luoyun Zhang Changhai Liang 《Journal of Energy Chemistry》 SCIE EI CAS CSCD 2018年第2期600-610,共11页
Hydrodeoxygenation(HDO) is an effective alternative to produce value-added chemicals and liquid fuels by removing oxygen from lignin-derived compounds. Sulfide catalysts have been proved to have good activity for th... Hydrodeoxygenation(HDO) is an effective alternative to produce value-added chemicals and liquid fuels by removing oxygen from lignin-derived compounds. Sulfide catalysts have been proved to have good activity for the HDO and particularly high selectivity to phenolic products. Herein, we presented a novel way to prepare the layered structure sulfide catalysts(MgFeMo-S) derived from MgFe hydrotalcites via the intercalation of Mo in consideration of the memory effect of the calcined hydrotalcite. By varying the Mg/Fe mole ratio, a series of MgFeMo-S catalysts were successfully prepared and characterized by nitrogen adsorption/desorption isotherms, X-ray diffraction(XRD), transmission electron microscopy(TEM),and inductively coupled plasma optical emission spectrometer(ICP-OES). The characterization results indicated that the MgFeMo-S catalyst has retained the unique layered structure, which can facilitate uniform dispersion of the MoS2 species on both the surface and interlayer of the catalysts. For the HDO of eugenol, the Mg1Fe2Mo-S catalysts exhibited the best HDO activity among all the catalysts due to its higher active metal contents and larger pore size. The HDO conversion was 99.6% and the yield of phenolics was 63.7%, under 5 MPa initial H2 pressure(measured at RT) at 300 ℃ for 3 h. More importantly,MoS2 species deposited on the interlayer galleries in the MgFeMo-S catalysts resulted in dramatically superior HDO activity to MoS2/Mg1Fe2-S catalyst. Based on the mechanism investigation for eugenol, the HDO reaction route of eugenol under sulfide catalytic system has been proposed for the first time. Further applicability of the catalyst on HDO of more lignin-derived compounds was operated, which showed good HDO activity and selectivity to produce aromatic products. 展开更多
关键词 MgFe Hydrotalcite Sulfide catalysts Layered structure HYDRODEOXYGENATION eugenol
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<i>In Vitro</i>Efficacy of Clove Oil and Eugenol against <i>Staphylococcus</i>spp and <i>Streptococcus mutans</i>on Hydrophobicity, Hemolysin Production and Biofilms and their Synergy with Antibiotics
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作者 Huma Jafri Iqbal Ahmad 《Advances in Microbiology》 2021年第2期117-143,共27页
The present study aimed to evaluate <i>Syzygium aromaticum</i> (clove) plant extract, clove oil and eugenol for their antibacterial activity and their potential to eradicate bacterial biofilms alone and in... The present study aimed to evaluate <i>Syzygium aromaticum</i> (clove) plant extract, clove oil and eugenol for their antibacterial activity and their potential to eradicate bacterial biofilms alone and in combination with antibiotics. Anti-bacterial efficacy of <i>S. aromaticum</i> extract, clove oil and eugenol was evaluated as minimum inhibitory concentration (MIC) and subsequently sub-MICs was selected for inhibition of virulence factors against test bacterial strains. Biofilm cultivation and eradication was assayed using XTT reduction in 96-well microtiter plate. Checkerboard method was used to study the interaction between essential oils and antibiotics. <i>Staphylococcus aureus</i> MTCC3160, <i>Staphylococcus epidermidis</i> MTCC435, <i>Staphylococcus sciuri</i> (SC-01), <i>Staphylococcus auricularis</i> (SU-01) <i>and</i> <i>Streptococcus mutans</i> MTCC497 were found strong biofilm former among all the test bacterial strains. The potency of test agents was found in the order of eugenol > clove oil > <i>S. aromaticum</i> methanolic extract. Sub-MIC (0.5 × MIC) of clove oil and eugenol showed a significant reduction in cell surface hydrophobicity (<i>p </i>< 0.05) and hemolysin production in the test bacterial strains. Eugenol showed no increase in sessile MIC (SMIC) against <i>S. auricularis</i> (SU-01), <i>S. epidermidis</i> MTCC435 and <i>S. mutans</i> MTCC497 compared to planktonic MIC (PMIC). Antibiotics (vancomycin and azithromycin) exhibited upto 1000-folds increased in SMIC compared to PMIC against all the test bacterial strains. Synergy was observed between eugenol and antibiotics (vancomycin/azithromycin) against all the test bacterial strains in both planktonic and sessile mode. Highest synergy was exhibited between eugenol and azithromycin in planktonic mode (FICI value 0.141). Further, microscopy also confirmed the spectacular effect of combination treatment on pre-formed <i>S. aureus</i> MTCC3160 and <i>S. mutans</i> MTCC497 biofilms. These findings highlighted the promising role of clove oil and eugenol alone and in combination on pathogenic bacterial biofilms. 展开更多
关键词 Syzygium aromaticum Staphylococcus spp Streptococcus mutans eugenol Biofilm Inhibition Hydrobhobicity SMIC SYNERGY
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Antiasthmatic Effect of Eugenol (4-Allyl-2-Methoxyophenol) Mediated by Both Bronchodilator and Immunomodulatory Properties
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作者 Campos Keina Maciele Teixeira Tatiane Oliveira +7 位作者 Cerqueira-Lima Ana Tereza Costa Ryan Santos CarneiroTamires Cana Brasil Silva Darizy Faivia Barreto Mauricio Lima Pontes-de-Carvalho Lain Carlos Alcantatara-Neves Neuza Maria Figueiredo Camila Alexandrina 《Journal of Pharmacy and Pharmacology》 2014年第1期38-49,共12页
Eugenol, an aromatic product, exhibits anti-inflammatory properties, however, little is known about its effect in allergic inflammation mediated by Th2-type cells and cytokines. We examined the pharmacological potenti... Eugenol, an aromatic product, exhibits anti-inflammatory properties, however, little is known about its effect in allergic inflammation mediated by Th2-type cells and cytokines. We examined the pharmacological potential of this compound in a murine model of respiratory allergy to Bt (Blomia tropicalis). For this, AJ mice were sensitized (100μg/animal s.c.) and challenged (10 μg/animal i.n.) with Bt mite extract. Sensitized animals were treated or not with eugenol (20, 40 or 80 mg/kg) and the following parameters were analyzed: number of total cells in BAL (bronchoalveolar lavage); EPO (eosinophil peroxidase activity) and histopathological changes in the lungs; serum level of specific IgE, IgG1 and IgG2a and; concentration of Th2 cytokines on BAL and spleen cultures. In addition, the capability ofeugenol in relaxing tracheal smooth muscle was also evaluated. Our results showed that treatment with eugenol significantly reduced the airway inflammation, decreasing the cellular infiltrate, EPO and mucus in the lungs, as well as the production of Th2 cytokines. It was also demonstrated a dilator effect of eugenol observed by the relaxation of normal and hyper-reactivity isolated trachea upon carbachol stimulation. These results suggest that eugenol has potential as an anti-asthmatic drug by both bronchodilator and immunomodulatory properties. 展开更多
关键词 ASTHMA BIomia tropicalis natural products eugenol.
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Complexation of Eugenol(EG),as Main Component of Clove Oil and as Pure Compound,withβ-and HP-β-CDs
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作者 Pilar Hernández-Sánchez Santiago López-Miranda +1 位作者 Carmen Lucas-Abellán Estrella Núnez-Delicado 《Food and Nutrition Sciences》 2012年第6期716-723,共8页
Eugenol, both in its pure form (EG) and included in essential clove oil (CO) was successfully solubilized in aqueous solution by forming inclusion complexes with β-cyclodextrins (β-CDs) and its modified hydroxy-prop... Eugenol, both in its pure form (EG) and included in essential clove oil (CO) was successfully solubilized in aqueous solution by forming inclusion complexes with β-cyclodextrins (β-CDs) and its modified hydroxy-propyl-β-CDs (HP-β-CDs). To investigate the molecular association between β-CDs/HP-β-CDs with pure EG and essential CO, phase solubility studies were undertaken. Essential CO formed insoluble complexes with β-CDs, but not with HP-β-CDs. The work clearly demonstrates complexes formation follow an order higher than 1:1 when high essential CO and β-CDs concentrations were used, however it was 1:1 in the case of essential CO-HP-β-CDs complexes. When pure EG was studied the results indicated that EG could form 1:1 inclusion complexes with β-CDs and HP-β-CDs. Based on the studies, the Kc values for pure EG were 4555 ± 225 M-1 and 10,633 ± 614 M-1 for β-CDs and HP-β-CDs, respectively, and 2005 ± 199 M-1 for essential CO-HP-β-CDs. These finding indicate that CDs are suitable for encapsulating EG. 展开更多
关键词 eugenol Essential Clove Oil β-CDs HP-β-CDs
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Evaluation of the antibacterial mechanism and biofilm removal effect of eugenol on Vibrio vulnificus and its application in fresh oysters
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作者 Kunyao Luo Shengnan Kang +5 位作者 Meixian Guo Chenyu Shen Lehui Wang Xiaodong Xia Xin Lü Chao Shi 《Food Bioscience》 SCIE 2022年第6期1092-1101,共10页
Eugenol is a natural active substance with high antibacterial activity,but its antibacterial activity against Vibrio vulnificus has not been extensively studied.The purpose of this study was to investigate the antibac... Eugenol is a natural active substance with high antibacterial activity,but its antibacterial activity against Vibrio vulnificus has not been extensively studied.The purpose of this study was to investigate the antibacterial and biofilm-clearing abilities and potential mechanisms of eugenol against V.vulnificus,and to provide knowledge for the use of eugenol to prevent oyster contamination.It was found that eugenol had an encouraging antibacterial effect on V.vulnificus with a minimum inhibitory concentration(MIC)of 0.2 mg/mL.The accumulation of reactive oxygen species(ROS)and the increase of malondialdehyde(MDA)content suggest that oxidative stress is involved in the bactericidal mechanism.Moreover,cell membrane hyperpolarization,changes in cell membrane integrity and morphology suggest that eugenol can reduce the permeability and integrity of cell membranes in V.vulnificus.In addition,eugenol produced a significant biofilm clearance effect on V.vulnificus,as evidenced by the reduced amount of biofilm and the reduction of polysaccharides and viable cells in the biofilm.Finally,eugenol was able to effectively inhibit the activity of V.vulnificus in artificially contaminated oyster at 4°C and 25°C.But sensory analysis showed that 0.10%eugenol was most acceptable to trained panelists.All of these highlight the great promise of eugenol as a natural bacteriostatic agent for the food industry. 展开更多
关键词 eugenol Vibrio vulnificus Antibacterial mechanism Biofilm removal Reactive oxidative species Oyster
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Evaluation of antibacterial and toxicological activities of essential oil of Ocimum gratissimum L.and its major constituent eugenol
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作者 Julio Cesar Silva Raimundo Luiz Silva Pereira +13 位作者 Thiago Sampaio de Freitas Janaína Esmeraldo Rocha Nair Silva Macedo Carla de Fatima Alves Nonato Marina Leite Linhares Daniely Sampaio Arruda Tavares Francisco Assis Bezerra da Cunha Henrique Douglas Melo Coutinho Sidney Gonçalo de Lima Francisco Nascimento Pereira-Junior Francisco Paulo Araújo Maia Ivo Cavalcante Pita Neto Fabiola Fernandes Galvão Rodrigues George Joaquim Garcia Santos 《Food Bioscience》 SCIE 2022年第6期2152-2159,共8页
The present study aimed to comparatively analyze the antibacterial activity,combined with antibiotics,and toxicological activity of the essential oil of O.gratissimum L.and its major compound,eugenol.The phytochemical... The present study aimed to comparatively analyze the antibacterial activity,combined with antibiotics,and toxicological activity of the essential oil of O.gratissimum L.and its major compound,eugenol.The phytochemical analysis of the essential oil was performed by gas chromatography coupled with mass spectrometry.The minimum inhibitory concentration and combined antibiotic activity were performed by microdilution.Drosophila melanogaster was the model organism chosen to evaluate the toxicological response of substances.The chromatographic assay showed eugenol as the major compound of the essential oil of O.gratissimum L.The microbiological assays showed that in terms of antibacterial activity,both products showed similar activity,quite significant in Gram-negatives,such as those of strains of Pseudomonas aeruginosa.Similar results were verified in the modulatory activity,allowing the verification that the bioactivity performed by the essential oil of O.gratissimum L.is mainly influenced by its major constituent.Although the EC50 of the compounds varied subtly,the essential oil was less toxic compared to eugenol.The information obtained in the present study demonstrates that the essential oil of O.gratissimum L.is a promising antibacterial agent and better tolerated than its majority compound alone,in the model organism D.melanogaster. 展开更多
关键词 Antibacterial agents Drosophila melanogaster eugenol Ocimum gratisismumum L.essential oils
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Extraction of Eugenol from Essential Oils by In Situ Formation of Deep Eutectic Solvents:A Green Recyclable Process
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作者 Lingqi Shen Xiangzi Jin +3 位作者 Zhihui Zhang Yuexing Yi Jingyu Zhang Zuguang Li 《Journal of Analysis and Testing》 EI CSCD 2024年第1期63-73,共11页
Eugenol is extensively utilized in chemical industry.In actual production,it is mainly separated from clove essential oils(EOs)through acid-base solutions.In this work,a simple,rapid and effective extraction process w... Eugenol is extensively utilized in chemical industry.In actual production,it is mainly separated from clove essential oils(EOs)through acid-base solutions.In this work,a simple,rapid and effective extraction process was developed for the extraction of eugenol from EOs samples by directly forming deep eutectic solvents(DESs)without using acid-base solutions.The proposed process is designed to dissolve EOs in n-hexane and eugenol was highly selective extracted into DESs with tetrabutylammonium chloride(TBAC).Hydrogen bonds in DESs can be broken by addition of water,resulting in a separation of the two phases,thus obtaining a hydrophobic yellowish eugenol in the upper layer.The quaternary ammonium salt in the lower aqueous solution can be reused after recovery.The most determining influence factors such as the structure of quaternary ammonium salt,the mole ratio of quaternary ammonium salt to eugenol and the amount of n-hexane were investigated.The forming conditions of DESs were studied by Box-Behnken design(BBD).Experiment was carried out under the optimal conditions and good experimental result was demonstrated by the developed process,a high extraction recovery of 92.51% and the purity was about 98.50%,which verifies the excellent performance for the proposed process. 展开更多
关键词 eugenol Deep eutectic solvent Essential oils Tetrabutylammonium chloride
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Quantification of eugenol and bancroftione in Caryophylli Fructus using high-performance liquid chromatography 被引量:1
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作者 刘淑娟 林宗涛 +1 位作者 王弘 陈世忠 《Journal of Chinese Pharmaceutical Sciences》 CAS 2010年第6期459-463,共5页
For the first time, we have utilized high-performance liquid chromatography (HPLC) to simultaneously quantify the eugenol and bancroffione in Caryophylli Fructus. The optimized parameters included: Inertsil ODS-4 c... For the first time, we have utilized high-performance liquid chromatography (HPLC) to simultaneously quantify the eugenol and bancroffione in Caryophylli Fructus. The optimized parameters included: Inertsil ODS-4 column (150 mm×4.6 mm, 5 μm); column temperature: 35 ℃; mobile phase: methanol water (65:35, v/v); flow rate: 1.0 mL/min; detection wavelength: 280 nm. Eugenol and bancroftione showed good linear relationships with peak areas within the range of (0.0998 0.8982) mg/mL (r = 0.9999) and (0.1474-1.3266) mg/mL (r = 0.9999), respectively. The average recoveries were 102.52% and 100.96% for eugenol and bancroftione, respectively. Our results showed that the established method is simple, rapid, and accurate with good reproducibility to evaluate the quality of Caryophylli Fructus. 展开更多
关键词 Caryophylli Fructus eugenol Bancroftione Quantification HPLC
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Synthesis of Eugenol Bio-based Reactive Epoxy Diluent and Study on the Curing Kinetics and Properties of the Epoxy Resin System 被引量:5
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作者 Bin Chen Feng Wang +3 位作者 Jing-Yu Li Jia-Lu Zhang Yan Zhang Hai-Chao Zhao 《Chinese Journal of Polymer Science》 SCIE CAS CSCD 2019年第5期500-508,共9页
In this study, monoglycidyl silyl etherated eugenol(GSE) was synthesized as reactive epoxy diluent, and the chemical structure of GSE, intermediates, and products were characterized by Fourier transform infrared spect... In this study, monoglycidyl silyl etherated eugenol(GSE) was synthesized as reactive epoxy diluent, and the chemical structure of GSE, intermediates, and products were characterized by Fourier transform infrared spectroscopy(FTIR) and nuclear magnetic resonance(~1 H-NMR). GSE existed as a potential bio-based reactive diluent for petroleum-based epoxy resin. The curing kinetics of EP/HHPA/GSE system was studied by non-isothermal DSC method. The kinetics parameters were calculated by using the Kissinger model, Crane model, Ozawa model, and β-T(temperature-heating rate) extrapolation, respectively. In addition, the effects of GSE on the thermo-mechanical properties and thermal stability of EP/HHPA/GSE systems were studied, indicating that GSE can effectively improve the toughness and thermal decomposition temperature of the epoxy system. 展开更多
关键词 Bio-based EPOXY REACTIVE DILUENT eugenol CURING kinetics
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A new insight into electrochemical detection of eugenol by hierarchical sheaf-like mesoporous NiCo2O4 被引量:3
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作者 Mylamparambil Udayan Anu Prathap Chao Wei Shengnan Sun Zhichuan J. Xu 《Nano Research》 SCIE EI CAS CSCD 2015年第8期2636-2645,共10页
NiCo2O4 nanosheets with sheaf-like nanostructure morphologies have been synthesized by a facile one-step hydrothermal reaction followed by annealing treatment. Impressively, the NiCo2O4 nanosheets exhibit rapid detect... NiCo2O4 nanosheets with sheaf-like nanostructure morphologies have been synthesized by a facile one-step hydrothermal reaction followed by annealing treatment. Impressively, the NiCo2O4 nanosheets exhibit rapid detection of eugenol. The linear range of detection is from 1-500μM, and the limit of detection is 5.4 μM. The NiCo2O4 modified electrode demonstrated high sensitivity, good repeatability and reproducibility, and long-term stability (7% decrease in response over 30 days). Based on this work, an electrochemical reaction mechanism for eugenol oxidation was proposed, and in addition, the NiCo2O4 modified electrode was successfully employed for the analysis of eugenol in medicative balm samples. Recovery values for eugenol in medicative balm samples were in the range 98.7%-105.5%. 展开更多
关键词 NiCo2O4 nanosheets eugenol determination ELECTROCATALYSIS electrochemical mechanism real sample analysis
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Optimizing methyl-eugenol aromatherapy to maximize posttreatment effects to enhance mating competitiveness of male Bactrocera carambolae (Diptera: Tephritidae) 被引量:1
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作者 Ihsan ul Haq Marc J.B. Vreysen +2 位作者 Carlos Caceres Todd E. Shelly Jorge Hendrichs 《Insect Science》 SCIE CAS CSCD 2015年第5期661-669,共9页
Methyl-eugenol (ME) (1,2-dimethoxy-4-(2-propenyl)benzene), a natural phy- tochemical, did enhance male Bactrocera carambolae Drew & Hancock (Diptera: Tephri- tidae) mating competitiveness 3 d after ingestion... Methyl-eugenol (ME) (1,2-dimethoxy-4-(2-propenyl)benzene), a natural phy- tochemical, did enhance male Bactrocera carambolae Drew & Hancock (Diptera: Tephri- tidae) mating competitiveness 3 d after ingestion. Enhanced male mating competitiveness can significantly increase the effectiveness of the sterile insect technique (SIT). ME appli- cation to mass reared sterile flies by feeding is infeasible. ME application by aromatherapy however, would be a very practical way of ME application in fly emergence and release facilities. This approach was shown to enhance mating competitiveness ofB. carambolae 3 d posttreatment (DPT). Despite this added benefit, every additional day of delaying re- lease will reduce sterile fly quality and will add cost to SIT application. The present study was planned to assess the effects of ME-aromatherapy on male B. carambolae mating competitiveness 1DPT and 2DPT. ME aromatherapy 1DPT or 2DPT did enhance mating competitiveness of B. carambolae males whereas ME feeding 1DPT and 2DPT did not. Male mating competitiveness was enhanced by the ME aromatherapy irrespective if they received 1DPT, 2DPT or 3DPT. ME aromatherapy, being a viable approach for its applica- tion, did enhance mating competitiveness of male B. carambolae 1 d posttreatrnent as ME feeding did 3 d after ingestion. 展开更多
关键词 Bactrocera carambolae mating competitiveness ME aromatherapy methyl-eugenol ME feeding posttreatment effect SIT
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Preparation of mesoporous silica nanoparticle with tunable pore diameters for encapsulating and slowly releasing eugenol
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作者 Tianlu Zhang Zhiguo Lu +8 位作者 Jianze Wang Jie Shen Qiulian Hao Yan Li Jun Yang Yunwei Niu Zuobing Xiao Lei Chen Xin Zhang 《Chinese Chemical Letters》 SCIE CAS CSCD 2021年第5期1755-1758,共4页
Fragrances are frequently added to a variety of products,including food,cosmetics and health products.However,the high volatility and instability of essence limit its application in some fields.In this study,mesoporou... Fragrances are frequently added to a variety of products,including food,cosmetics and health products.However,the high volatility and instability of essence limit its application in some fields.In this study,mesoporous silica nanoparticles(MSNs) were prepared to encapsulate eugenol,which could reduce the volatilization of the fragrance molecules.A facile approach was presented to synthesize MSNs with three different pore diameters for encapsulating eugenol.In addition,the properties of MSNs including mean particle size,morphology,encapsulating efficiency and release tendency were charac terized.Results showed that the larger the pore diameters of MSNs,the more aromatic molecules were adsorbed.Furthermore,the release mechanism was described as the smaller the pore diameters of MSNs,the slower the release of eugenol. 展开更多
关键词 eugenol Mesoporous silica nanoparticles Nano-fragrance Slow release of fragrance
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Cationic and temperature-sensitive liposomes loaded with eugenol for the application to silk
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作者 Zhiguo Lu Xiangyu Wang +9 位作者 Tianlu Zhang Luyao Zhang Jun Yang Yan Li Jie Shen Jianze Wang Yunwei Niu Zuobing Xiao Guiying Liu Xin Zhang 《Chinese Chemical Letters》 SCIE CAS CSCD 2020年第12期3139-3142,共4页
Silk has been widely used in the clothing industry due to their soft and smooth features,good biocompatibility,good heat dissipation,warmth and ultraviolet resistance.The application of fragrance to silk can significa... Silk has been widely used in the clothing industry due to their soft and smooth features,good biocompatibility,good heat dissipation,warmth and ultraviolet resistance.The application of fragrance to silk can significantly improve the performance of silk.However,there are two key scientific problems that need to be solved:slowing down the release rate of fragrances and increasing the scent lasting time of silk.In this study,cationic and temperature-sensitive liposomes were designed and prepared to encapsulate eugenol.These fragrance-loaded liposomes significantly slowed down the release rate of the fragrance and controlled the release rate of the fragrance in a thermo-sensitive manner.The liposomes adhered to the silk through electrostatic adsorption interaction.The positive charge on the fragranceloaded liposomes neutralized much negative charge on silk and thereby increasing the adhesion efficiency. 展开更多
关键词 eugenol SILK Cationic and temperature-sensitive liposomes Slow release of fragrance Controlled release of fragrance
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Preparation of hollow mesoporous silica nanorods for encapsulating and slowly releasing eugenol
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作者 Tianlu Zhang Zhiguo Lu +8 位作者 Luyao Zhang Yan Li Jun Yang Jie Shen Jianze Wang Yunwei Niu Zuobing Xiao Lei Chen Xin Zhang 《Chinese Chemical Letters》 SCIE CAS CSCD 2020年第12期3135-3138,共4页
Fragrances are widely used in many aspects of our lives.They cannot only make people happy,but also treat many diseases.However,excessively fast evaporation rate is one of the main obstacles to the use of spices.In th... Fragrances are widely used in many aspects of our lives.They cannot only make people happy,but also treat many diseases.However,excessively fast evaporation rate is one of the main obstacles to the use of spices.In this study,mesoporous silica nanorods(MSNRs)and hollow mesoporous silica nanorods(HMSNRs)were prepared to encapsulate eugenol.These two nano-fragrances were named eugenol@MSNRs and eugenol@HMSNRs,respectively.The morphologies,size,interior structures and pore performances of MSNRs and HMSNRs.Besides,the performances of encapsulation and fragrance release of eugenol@MSNRs and eugenol@HMSNRs were compared and analyzed.The results showed that eugenol@HMSNRs encapsulated more fragrance and were faster to encapsulate compared with eugenol@MSNRs.Both the release rates of eugenol from eugenol@MSNRs and eugenol@HMSNRs were slow.But the eugenol was released from eugenol@MSNRs more slowly. 展开更多
关键词 eugenol Hollow mesoporous silica nanorods Mesoporous silica nanorods Nano-fragrance Slow release of fragrance
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Synthesis, Crystal Structure and Biological Activities of Naproxen-eugenol Ester Prodrug
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作者 LIANG Di YANG Xiao-hong +4 位作者 SUN Wei WANG Wen-na YANG Jin-zhu LIU yin-yan WANG Guang-shu 《Chemical Research in Chinese Universities》 SCIE CAS CSCD 2013年第2期245-248,共4页
The prodrug, naproxen-eugenol ester, was synthesized by acyl chloride method with naproxen and eugenol as the raw materials. The structure was identified by proton nuclear magnetic resonance(~H NMR), mass spectrome... The prodrug, naproxen-eugenol ester, was synthesized by acyl chloride method with naproxen and eugenol as the raw materials. The structure was identified by proton nuclear magnetic resonance(~H NMR), mass spectrometry(MS), infrared spectrometry(IR) and X-ray diffraction. The compound was crystallized in the or- thorhombic system, space group P21212t with unit cell dimensions a=0.60563(12) nm, b=1.0234(2) nm, c=3.2654(7) nm, a=90°, β=90°, γ=90°, V=2.0240(7) nm3, Z=4. Calculated density 1.235 Mg/m3; absorption coefficient: 0.083 mm-1; F(000)=800; final R1=0.0564. The analgesic activity and anti-inflammatory were similar to those of naproxen, and the results of ulcerogenic activity indicate that the prodrug can significantly decrease the irritation after oral ad- ministration. 展开更多
关键词 Naproxen-eugenol ester Crystal structure Biological activity
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