Effect of Inhibinα(1-32)Gene Immunization on the Follicular Development and Reproductive Hormones in Rats

A total of 40 rats, aged in 30 days. were divided into 4 groups and immunized (intramuscularly injection) with 0 μg(control), 15μg (group 1), 25μg (group 2) or 40 μg (group 3) of inhibin α(1-32) re-combinant expression plasmid pcINH in combination with liposome. Booster was given without liposome on day 20 after primary immunization. The results showed that 50%(13/26) rats were detected in positive antibody against inhibin. However, the increase of immunization dosage and booster did not promote the ratio of antibody positive rats. The number of matured follicles above 0.8 mm in diameter in the antibody positive rats was 2.3 more than that in the negative rats (P>0. 05). The concentration of blood plasma FSH increased distinctively on day 10 after primary immunization (P<0. 05), but no increase was observed after booster immunization. The 17-β-estradiol levels in blood plasma of rats between the positive and the negative groups had no remarkable differences (P>0.05). These results suggested that recombinant inhibin expression plasmid could stimulate animal body to produce antibody against inhibin.

Multi‑omics analysis reveals gut microbiota‑ovary axis contributed to the follicular development difference between Meishan and Landrace×Yorkshire sows

Background The mechanism by which Meishan(MS)sows are superior to white crossbred sows in ovarian follicle development remains unclear.Given gut microbiota could regulate female ovarian function and reproductive capacity,this study aimed to determine the role of gut microbiota-ovary axis on follicular development in sows.Methods We compared the ovarian follicular development,gut microbiota,plasma metabolome,and follicular fluid metabolome between MS and Landrace×Yorkshire(L×Y)sows.A H_(2)O_(2)-induced cell apoptosis model was used to evaluate the effects of multi-omics identified metabolites on the apoptosis of porcine ovarian granulosa cells in vitro.Results Compared with L×Y sows,MS sows have greater ovary weight and improved follicular development,including the greater counts of large follicles of diameter≥5 mm,secondary follicles,and antral follicles,but lesser atretic follicles.The ovarian granulosa cells in MS sows had alleviated apoptosis,which was indicated by the increased BCL-2,decreased caspases-3,and decreased cleaved caspases-3 than in L×Y sows.The ovarian follicular fluid of MS sows had higher concentrations of estradiol,progesterone,follicle-stimulating hormone,luteinizing hormone,and insulin like growth factor 1 than L×Y sows.Gut microbiota of MS sows formed a distinct cluster and had improved alpha diversity,including increased Shannon and decreased Simpson than those of L×Y sows.Corresponding to the enhanced function of carbohydrate metabolism and elevated short-chain fatty acids(SCFAs)in feces,the differential metabolites in plasma between MS and L×Y sows are also mainly enriched in pathways of fatty acid metabolism.There were significant correlations among SCFAs with follicular development,ovarian granulosa cells apoptosis,and follicular fluid hormones,respectively.Noteworthily,compared with L×Y sows,MS sows had higher follicular fluid SCFAs concentrations which could ameliorate H_(2)O_(2)-induced porcine granulosa cells apoptosis in vitro.Conclusion MS sows have more secondary and antral follicles,but fewer atretic follicles and apoptotic ovarian granulosa cells,as well as harbored a distinctive gut microbiota than L×Y sows.Gut microbiota may participate in regulating ovarian follicular development via SCFAs affecting granulosa cells apoptosis in sows.

Major royal jelly proteins accelerate onset of puberty and promote ovarian follicular development in immature female mice

The major royal jelly proteins(MRJPs)are the central constituents responsible for the specific activities of royal jelly.Here MRJPs via oral administration daily for 45 consecutive days were evaluated the effects on the reproductive parameters in immature female mice(FM).Neonatal FM were divided into four groups fed MRJPs with doses of 0,125,250 and 500 mg/kg/body weight(M125,M250 and M500).The results in M125,M250 and M500 showed that the times of estrus were accelerated by 10.7%,15.5%and 10.7%,the secondary follicles number were increased by 50.7%,78.8%and 38.6%,the Graafian follicles were increased by 600.0%and 774.0%and 150.0%,respectively.M500 induced multi-oocyte follicles.The serum estradiol levels of the three groups were increased by 47.1%,64.9%and 31.1%,the action of MRJPs raising hormone secretion level is mainly via upregulating expression of ERˇgene.Antioxidant parameters of ovarian tissue showed that the malondialdehyde levels in M125 and M250 were decreased,the superoxide dismutase activities and glutathione peroxidase activities in M125 and M250 were increased.In conclusion,MRJPs may accelerate onset of puberty and promote follicular development in FM.Our findings would facilitate better understanding of the benefit effect of MRJPs as the key ingredient in royal jelly on promoting fertility performance.

Beneficial effects of a decreased meal frequency on nutrient utilization,secretion of luteinizing hormones and ovarian follicular development in gilts

Background:Replacement gilts are typically fed ad libitum,whereas emerging evidence from human and rodent studies has revealed that time-restricted access to food has health benefits.The objective of this study was to investigate the effect of meal frequency on the metabolic status and ovarian follicular development in gilts.Methods:A total of 36 gilts(Landrace×Yorkshire)with an age of 150±3 d and a body weight of 77.6±3.8 kg were randomly allocated into one of three groups(n=12 in each group),and based on the group allocation,the gilts were fed at a frequency of one meal(T1),two meals(T2),or six meals per day(T6)for 14 consecutive weeks.The effects of the meal frequency on growth preference,nutrient utilization,short-chain fatty acid production by gut microbial,the post-meal dynamics in the metabolic status,reproductive hormone secretions,and ovarian follicular development in the gilts were measured.Results:The gilts in the T1 group presented a higher average daily gain(+48 g/d,P<0.05)and a higher body weight(+4.9 kg,P<0.05)than those in the T6 group.The meal frequency had no effect on the apparent digestibility of dry matter,crude protein,ether extract,ash,and gross energy,with the exception that the T1 gilts exhibited a greater NDF digestibility than the T6 gilts(P<0.05).The nitrogen balance analysis revealed that the T1 gilts presented decreased urine excretion of nitrogen(−8.17 g/d,P<0.05)and higher nitrogen retention(+9.81 g/d,P<0.05),and thus exhibited higher nitrogen utilization than the T6 gilts.The time-course dynamics of glucose,α-amino nitrogen,urea,lactate,and insulin levels in serum revealed that the T1 group exhibited higher utilization of nutrients after a meal than the T2 or T6 gilts.The T1 gilts also had a higher acetate content and SCFAs in feces than the T6 gilts(P<0.05).The age,body weight and backfat thickness of the gilts at first estrous expression were not affected by the meal frequency,but the gilts in the T1 group had higher levels of serum luteinizing hormone on the 18th day of the 3rd estrus cycle and 17β-estradiol,a larger number of growing follicles and corpora lutea,and higher mRNA expression levels of genes related to follicular development on the 19th day of the 3rd estrus cycle.Conclusions:The current findings revealed the benefits of a lower meal frequency equal feed intake on nutrient utilization and reproductive function in replacement gilts,and thus provide new insights into the nutritional strategy for replacement gilts,and the dietary pattern for other mammals,such as humans.

Progress in Study on Shen-invigorating Herbs Used in Promoting Follicular Development

The practice of stimulating follicular develop-ment by Shen-invigorating herbs is directed by tra-ditional Chinese medical theories of“the Shenstores essence of life and governs reproduction”

Effects of Heyan Kuntai Capsule(和颜坤泰胶囊)on Follicular Development and Oocyte Cohesin Levels in Aged Mice

Objective： To evaluate the effect of Heyan Kuntai Capsule （和颜坤泰胶囊, HYKT） on the ovarian function of aged mice and expressions of cohesion complexes in oocytes. Methods： Twenty-five 9-month-old female C57BL/6J mice were randomly divided into 5 groups by block randomization method （n=5 per group）, including the control group （saline）, 17β-estradiol group [E2, 100 μg/（kg·d）], and low-, medium-, and high- dose of HYKT groups [0.3, 0.9, 2.7 g/（kg·d）, respectively]. All mice were treated by intragastric administration for 4 weeks. Hematoxylin and eosin staining and anti-VASA staining were used to detect the amounts of follicles. The apoptosis of follicles was measured by anti-gamma H2A histone family member X （γ, H2AX） staining and TdT-mediated dUTP Nick-End Labeling （TUNEL） assay. The density of cohesin subunits, REC8 meiotic recombination protein （REC8）, structural maintenance of chromosome （SMC） 1 β and SMC3 in oocytes were evaluated by immunofluorescent staining. Results： After administration of E2 and high-dose of HYKT, the total number of follicles as well as the number of primordial and primary follicles were significantly increased （P〈0.05）. Anti-γ/H2AX staining and TUNEL assay demonstrated that high-dose of HYKT and E2 partly suppressed the apoptosis of follicles （P〈0.05）. Furthermore, it showed an increased trend in the levels of REC8 and SMC1 β, after administration with E2 and HYKT, and no obvious change in the level of SMC3. Conclusion： HYKT could enhance the number of follicles, suppress apoptosis of oocytes and have a trend to elevate the meiotic-specific cohesin subunits （REC8 and SMC1 β） in oocytes of aged mice, indicating a beneficial effect on the ovarian function in terms of the quantity and quality of follicles.

Effect of Epidermal Growth Factor on Follicular Development and Steroidogenesis in Perfused Rat Ovary

The effect of epidermal growth factor(EGF) on follicular development and steroidogenesis was investigated using an in vitro perfused immature rat ovary model. Type 3a and type 3b follicles were counted in the sections of perfused ovaries. To determine action of EGF on amortize activity, effect of testosterone(10-5 M) plus EGF(10 ng/ml) and that of testosterone(10-5 M) alone were compared. EGF(10 ng/ml) significantly increased the number of both types of follicles. At time-course experiment, EGF(10 ng/ml) did not enhance the number of both types of follicles after 10 hr perfusion, but significantly augmented it after perfusion of 20 hrs. EGF(10 ng/m) also stimulated progesterone production. There was no significant difference in estradiol level when ovaries were treated with EGF alone. The addition of testosterone significantly increased estradiol production. EGF inhibited testosterone-derived ovary estradiol production. These results suggest that EGF induces development of the primordial and primary follicles and plays an important role in controlling development by regulating function of granulosa cells.

基于转录组学探讨林蛙油对卵泡发育障碍大鼠的影响

目的:通过转录组学角度分析林蛙油对环磷酰胺所致卵泡发育障碍的影响,为林蛙油的雌激素样作用机制研究提供充足的理论及实验依据。方法:筛选后的雌性Wistar大鼠60只,随机分为对照组(C)、模型组(M)、阳性药组(P)、林蛙油低(ORL)、高剂量组(ORH);除对照组外,其余大鼠均腹腔注射环磷酰胺建立卵泡发育障碍大鼠模型;除模型组外,其余大鼠灌胃相应药物,连续灌胃14~16 d后测定大鼠血清性激素含量;摘取卵巢和子宫称量记录,计算器官指数;一侧卵巢与子宫做HE染色计算各级卵泡数量及子宫内膜厚度,另一侧卵巢进行转录组学分析,筛选差异基因,对差异基因进行GO和KEGG富集分析。结果:大鼠卵泡发育障碍模型成功建立。与模型组大鼠相比,林蛙油低、高剂量组大鼠子宫湿重和子宫指数显著升高(P<0.05),孕酮和雌二醇含量显著升高(P<0.05);林蛙油低、高剂量组大鼠窦前卵泡与黄体数量显著升高(P<0.05),子宫内膜厚度显著增加(P<0.01),差异有统计学意义,林蛙油低剂量组大鼠窦状卵泡数量显著升高(P<0.05);转录组结果显示,林蛙油低、高剂量组与模型组之间差异基因分别有738、572个;GO富集分析分别富集到1790、1616个条目;KEGG富集分析主要集中在PI3K/Akt信号通路与B细胞受体信号通路。结论:林蛙油能有效改善环磷酰胺所致卵泡发育障碍大鼠的血清性激素水平、卵巢卵泡发育情况、子宫内膜萎缩等情况,其分子机制可能与PI3K/Akt、B细胞受体等通路及基因有关。

Kisspeptin/kiss1r系统在多囊卵巢综合征大鼠卵巢中的表达及其对卵泡发育障碍的可能作用机制

目的：探讨多囊卵巢综合征（PCOS）大鼠卵巢中kisspeptin／kisslr系统的表达及其对PCOS大鼠卵泡发育障碍的可能作用机制。方法：构建PCOS大鼠模型；免疫组织化学检测实验大鼠卵巢中kisspeptin／kisslr的表达；免疫印迹和qRT-PCR分别检测kisspeptin／kisslr蛋白和mRNA水平。结果：与正常组相比，PCOS组的体质量及卵巢质量明显增加，血清雄激素、黄体生成素水平明显增加，卵泡刺激素水平变化无统计学差异。PCOS大鼠卵巢中含有较多发育早期的小卵泡及闭锁卵泡，囊状扩张卵泡明显增加，颗粒细胞层数减少至2～3层，黄体数量明显下降；hsspeptin／kissh蛋白和mRNA水平明显降低。结论：卵巢表达的kisspeptin／kisslr在PCOS大鼠卵泡发育障碍中可能通过调节颗粒细胞发挥作用，本研究将为进一步探讨PCOS的发病机制提供一定的理论基础。

体外受精周期中卵泡发育阻滞患者应用未成熟卵体外成熟培养的临床观察

目的观察常规体外受精(IVF)中卵泡发育阻滞患者采用未成熟卵体外成熟(IVM)培养治疗结果。方法常规IVF治疗患者11例,在促排卵过程发生卵泡发育阻滞时,取卵行IVM培养成熟后,卵母细胞行卵胞浆内单精子注射(ICSI)以获得受精卵并最终实现胚胎移植。统计分析未成熟卵的成熟率、卵母细胞的受精率、胚胎的发育情况及临床结果。结果共获取卵母细胞128枚,其中成熟卵21枚,行ICSI后15枚正常受精,获10枚正常卵裂胚胎,全部移植3例,均未妊娠;107枚未成熟卵,经IVM、ICSI和体外培养后,成熟率、受精率、正常卵裂率及优质胚胎率分别为72.9%、75.6%、78%及39.1%,胚胎移植后3例临床妊娠(新鲜周期2例,冻融周期1例)。结论常规IVF控制性超促排卵(COH)过程中,发育阻滞的卵泡在体外适宜的培养条件下仍有成熟、受精、继续发育的能力,并最终实现临床妊娠。IVM技术是改善IVF中发生卵泡发育阻滞患者结局的措施之一。

miR-99a-5p inhibits target gene FZD5 expression and steroid hormone secretion from goat ovarian granulosa cells

MicroRNA(miRNA) has vital regulatory effects on the proliferation, differentiation and secretion of ovarian granulosa cells, but the role of miR-99a-5p in goat ovarian granulosa cells(GCs) is unclear. Both miR-99a-5p and Frizzled-5(FZD5) were found to be expressed in GCs in goat ovaries via fluorescence in situ hybridization and immunohistochemistry, respectively, and FZD5 was verified(P<0.001) as a target gene of miR-99a-5p by double luciferase reporter gene experiments. Furthermore, FZD5 mRNA and protein expression were both found to be regulated(P<0.05) by miR-99a-5p in GCs. Moreover, the overexpression of miR-99a-5p or knockdown of FZD5 suppressed(P<0.05) estradiol and progesterone secretion from the GCs, as determined by ELISA. In summary, miR-99a-5p inhibits target gene FZD5 expression and estradiol and progesterone synthesis in GCs. Our study thus provides seminal data and new insights into the regulatory mechanisms of follicular development in the goat and other animals.

不同冷冻方法对牛卵泡卵母细胞发育潜力的影响

本试验探讨了不同方法冷冻保存的牛卵泡卵母细胞体外受精后的发育潜力。尽管用不同方法冷冻的牛卵母细胞大多 ( 79.1 % )形态正常 ,但体外受精后的受精率 ( 1 3.2 % )和卵裂率( 8.8% )均明显低于对照组 ( 66.7%和 46.0 % ) ,卵裂后继续发育的能力严重受损。在 3种方法中 ,玻璃化冷冻效果最好 ,受精率分别达到 1 5 .0 %和 1 1 .1 % 。

发育阶段对牛卵泡卵母细胞冷冻效果的影响

研究了用3种不同方法(A法:程序冷冻法;B法:超快速冷冻法;C法:玻璃化冷冻法)冷冻保存的牛GV期和IVM卵母细胞的发育潜力。结果表明,无论采取哪种方法,GV期卵母细胞的发育潜力均显著低于IVM卵母细胞;三种冷冻方法中,以玻璃化冷冻为最佳。

Intermittent spontaneous ovulation in patients with premature ovarian failure:Three case reports and review of literature

BACKGROUND Premature ovarian failure(POF)is the end-stage of a decline in ovarian function prior to the age of 40 years that involves symptoms associated with low estradiol(E2)levels and a minimal probability of pregnancy.This increases the physical and psychological burden experienced by young women of reproductive age,particularly with regards to over-diagnosis.CASE SUMMARY Here,we report three cases(29,22,and 33 years-of-age)diagnosed with POF after experiencing secondary amenorrhea for more than one year,serum levels of follicle-stimulating hormone(FSH)>40 IU/L on two occasions with an interval of more than 4 wk,and negative progesterone withdrawal tests.All three patients were intermittently administered with drugs to create an artificial cycle.During the subsequent discontinuation period,the patients experienced intermittent follicular growth and spontaneous ovulation.One patient experienced two natural pregnancies(both with embryo arrest).CONCLUSION Our findings suggest that young patients with POF can experience unpredictable and intermittent spontaneous follicular development,ovulation,and even natural pregnancy.Clinicians should provide appropriate medical guidance and individualized treatments according to fertility requirements,genetic risks and hypoestrogenic symptoms as soon as possible.

林蛙油对雷公藤所致大鼠卵泡发育障碍的影响

目的:研究林蛙油对卵泡发育障碍模型大鼠卵巢中的卵泡和磷脂酰肌醇受体3-酪氨酸激酶(phosphatidylinositol 3-kinase,PI3K)/卵蛋白激酶受体B(protein kinase B,Akt)的信号通路的影响。方法:筛选后的雌性WISTAR大鼠40只,随机分为对照组、模型组、阳性药组和林蛙油高、低剂量组;除对照组外其余各组大鼠每日灌胃雷公藤多苷溶液(40 mg/kg),阳性药组大鼠灌胃戊酸雌二醇溶液(0.1 mg/kg)和醋酸甲地孕酮溶液(0.8 mg/kg),林蛙油高、低剂量组大鼠分别灌胃林蛙油溶液(400、200 mg/kg)。第8周测定大鼠血清雌二醇(estradiol,E2)、孕酮(progesterone,P)、促黄体生成素(Luteinizing hormone,LH)、促卵泡生成素(Follicle-stimulating hormone,FSH)和睾酮(testosterone,T)含量;摘取卵巢和子宫称量记录,计算器官指数;一侧卵巢做HE染色和TUNEL染色计算各级卵泡数量。另外一侧卵巢测定其中PI3K、AKT、人第10号染色体缺失的磷酸酶(phosphatase and tensin homolog deleted on chromosome ten,PTEN)和哺乳动物雷帕霉素靶蛋白(mammalian target of rapamycin,mTOR)的mRNA相对表达量以及PI3K、磷酸化蛋白激酶B(phosphorylated protein kinase B,p-Akt)的蛋白含量。结果:大鼠卵泡发育障碍模型成功建立。与模型组大鼠相比,林蛙油高剂量组大鼠子宫湿重和子宫指数显著升高(P<0.05);林蛙油高、低剂量组大鼠发情周期显著缩短(P<0.05),次级卵泡数量显著升高(P<0.05),卵泡闭锁率显著降低(P<0.05),血清FSH含量显著减少(P<0.05)、T和E2含量显著升高(P<0.05),PI3K蛋白含量显著增多(P<0.05),Akt蛋白磷酸化水平显著提高(P<0.05),PTEN m RNA相对表达量显著减少(P<0.05);林蛙油高剂量组大鼠卵巢中mTOR mRNA相对表达量显著低于模型组大鼠(P<0.05)。结论:林蛙油对雷公藤多苷所致大鼠卵泡发育障碍有明显的改善作用,林蛙油可以上调PI3K/Akt信号通路促进卵泡发育障碍的大鼠卵泡的生长和发育。

周惠芳治疗卵泡发育障碍性不孕症经验

周惠芳顺应女子月经周期变化,治疗卵泡发育障碍性不孕症以滋养肝肾为主,结合经后健脾滋阴,排卵期活血通络,排卵后助阳的补肾调周法,在临证中,常见到一些辨证属脾肾阳虚的患者,辨证与调周相矛盾,周师认为,阴阳互根,阳中求阴,此时在经后期补阴的同时,适当加用小剂量的补阳药,如菟丝子、川续断,往往收到立竿见影之效。周师常在中医辨证论证的基础上配合西药治疗,加强疗效。周师在予以中药调理的同时,常耐心细致的与患者沟通交流,细心倾听,了解其内心苦闷,适时劝导,让其充分了解自己的病情,放松心情,保持精神心境舒畅,患者在解除精神负担后获得不错的治疗效果。

Induction of follicular luteinization by equine chorionic gonadotropin in cyclic guinea pigs

The effects of equine chorionic gonadotropin （eCG） on follicular development and ovulation in cyclic guinea pigs were investigated by histological and immunohistochemical analyses. Three groups of guinea pigs （n=12） were administrated subcutaneously with saline, 20 or 50 IU of eCG, respectively, on cyclic Day 12 （Day 1=vaginal openings）. Ovaries were collected at 4 and 8 d after administration （6 animals per group each time）. The eCG administration induced significant and distinct morphological changes in the ovaries, as it promoted the luteinization of granulosa cells, but not follicular development. In addition, proliferating cell nuclear antigen （PCNA） and steroidogenic acute regulatory protein （STAR） were immunolocalized specifically in luteinized follicles. Our experiments together indicate that eCG administration can induce follicular luteinization but not superovulation in guinea pigs. The eCG in cyclic guinea pigs functions similar to that of luteinizing hormone （LH）, but not follicle-stimulating hormone （FSH）.

未成熟卵体外成熟技术在卵巢反应不良和卵泡发育迟缓周期中的应用

目的探讨利用未成熟卵体外成熟技术治疗卵巢反应不良和卵泡发育迟缓周期的可能性.方法2000年10月至2002年7月间,在南京医科大学第一附属医院生殖中心取在常规体外受精(IVF)刺激周期中卵巢反应不良和卵泡发育迟缓患者的生发泡期卵母细胞,体外成熟培养24～48h后,对排出第一极体的卵母细胞采用单精子卵浆内注射法(ICSI)受精,受精3d后移入患者子宫,移植后通过检查血清HCG含量和B超判断妊娠结局.结果8个周期共取生发泡期卵母细胞41枚,成熟培养后有33枚卵母细胞排出第一极体,体外成熟率为80.5%(33/41).ICSI后,正常受精率为78.8%(26/33).平均每周期移植胚胎2.5(20/8)枚,获得3例妊娠.结论未成熟卵体外成熟技术可以用来挽救常规IVF治疗中卵巢反应不良和卵泡发育迟缓的周期.

Effects of Matricaria chamomilla Extract on Growth and Maturation of Isolated Mouse Ovarian Follicles in a Three-dimensional Culture System

Background： The aim of this study was to design and assess the effects of hydroalcoholic extract of Matricaria chamomill^l （MC） on preantral follicle culture of mouse ovaries in a three-dimensional culture system. Methods： Isolated preantral follicles were randomly divided into three main groups： the control group containing 10% fetal bovine serum without MC extract （G1）, the first experimental group supplemented with 25 ktg/ml hydroalcoholic extract of chamomile （G2）, and the second experimental group supplemented with 50 p,g/ml hydroalcoholic extract of chamomile （G3）. Results： After 12 days of culture, the survival rate （P 〈 0.05）, antrum formation （P 〈 0.01）, metaphase two oocytes （P 〈 0.01）, and the expression ofPCNA （P 〈 0.05） and FSHR （P 〈 0.05） genes significantly decreased in G3 as compared with GI. On the other hand, at the last day of culture （day 12）, the mean diameter of follicles cultured in the medium which was supplemented with 50 lag/ml hydroalcoholic extract of chamomile significantly decreased as compared with the GI （P 〈 0.05）. In addition, the levels of progesterone and dehydroepiandrosterone hormones significantly increased in the medium of G3 relative to G I （P 〈 0.01）, while in the medium of G 1, the level of 17[3-estradiol was significantly higher than that of other groups （P 〈 0.01）. Reactive oxygen species levels of metaphase 11 oocytes were significantly decreased in G2 as compared with G1 （P 〈 0.01）. Conclusion： Adding chamomile extract to culture media appeared to decrease follicular function and development.

反复IVF失败患者卵泡液代谢组学的检测分析

目的:探讨与卵子发育潜能相关的代谢物质。方法:运用气相色谱-质谱仪(GC-MS)回顾性分析13例反复IVF失败(RIF组)和15例同期第1次行IVF-ET成功妊娠患者(对照组)的卵泡液代谢组学。结果:用偏最小二乘法分析样本主成分,结果显示RIF组和对照组的主成分积分值集中分布于2个不同区域,且反复IVF失败组较对照组有11种氨基酸含量显著升高,而二元羧酸、胆固醇及部分有机酸含量显著降低。结论:升高的缬氨酸、苏氨酸、异亮氨酸、半胱氨酸、丝氨酸、脯氨酸、丙氨酸、苯丙氨酸、赖氨酸、甲硫氨酸、鸟氨酸,和降低的二元羧酸、胆固醇可能成为预测卵子发育潜能的代谢标志物。