[Objectives]This trial was conducted to investigate the effects of diet with"Lianteng"additive of Chinese herbal medicine on blood biochemical indexes,immune and antioxidant function of Guangxi Sanhuang chic...[Objectives]This trial was conducted to investigate the effects of diet with"Lianteng"additive of Chinese herbal medicine on blood biochemical indexes,immune and antioxidant function of Guangxi Sanhuang chickens.[Methods]480 Guangxi Sanhuang chickens at the age of 1 d were divided into 4 groups randomly and each group was assigned to 6 replicates with 20 chicks in each replicate;groups A,B and C were treatment groups and fed basal diets with 0.1%,0.2%and 0.4%of"Lianteng"additive of Chinese herbal medicine respectively during a trial of 70 d.Group D was control group and fed only basal diet.The serum samples were taken from 6 chicks in each group at the age of 21,35 and 49 d respectively and analyzed for biochemical,immune and antioxidant parameters in serum.[Results]Results from the trial showed that no difference was found in serum content of GLU,TG,TP,TC,and UN in the measurement at the age of 21,35 and 49 d(P>0.05);however,the content of ALB from groups A and B at 35 d was significantly higher than that from group D(P<0.01),and the ALB content from group B at 49 d was also higher than that from group D(P<0.05);the IgM content at 49 d from group A was lower than that from group D(P<0.05),in addition to this,there was no differences found in the contents of IgG,IgM and IFN-y among groups A,B,C and D during the trial(P>0.05);but the content of IL-2 from groups A and B at 49 d was significantly greater than that from group D(P<0.05);T-AOC from groups A,B and C at 49 d was very significantly higher than that from group D(P<0.01),and the one of group B at 35 d was still obviously higher than that of group D(P<0.05);also at 49 d,the GSH content in groups A and B was much greater than that in group D(P<0.01);T-SOD activity of group B and C at 35 d was distinctly greater than that of group D(P<0.05).[Conclusions]It can be concluded that the addition of 0.2%of"Lianteng"additive of Chinese herbal medicine in diet can increase immune function in a certain extent and promote the antioxidant ability observably for Guangxi Sanhuang chickens.展开更多
[ Objective] To compare immune efficacy of avian influenza virus （AIV） Re-5 strain （HSN1 subtype） and SS strain （ H9 subtype） in Guangxi Sanhuang chickens. [Method] A total of 180 Sanhuang chickens were selected...[ Objective] To compare immune efficacy of avian influenza virus （AIV） Re-5 strain （HSN1 subtype） and SS strain （ H9 subtype） in Guangxi Sanhuang chickens. [Method] A total of 180 Sanhuang chickens were selected from a breeding chicken farm in Guilin City of Guangxi and assigned into four groups （ n = 45）. At 145 days old, the chickens in the group I and II were respectively inoculated with AIV Re-5 strain and AIV SS strain, but they were kept in cages separately. The two control groups were not inoculated. Antibody levels were determined at 170 days old. [ Result] The level of antibodies against AIV Re-5 strain was significantly higher in the chickens of the group I and group II than in the corresponding control groups, respectively （ P 〈 0.01 ）. The level of antibodies against AIV SS strain was not significantly different between the group I and its control group or between the group II and its control group. [ Conclusion] The antibody level of chickens can be increased by the AIV Re-5 strain but not by the AIV SS strain.展开更多
[Objective] This trial was to investigate the effect of dietary Picria felterrae Lour extracts on serum biochemical indexes and immune function of Guangxi Partridge chickens. [Method] Totally 500 individuals of 1-day-...[Objective] This trial was to investigate the effect of dietary Picria felterrae Lour extracts on serum biochemical indexes and immune function of Guangxi Partridge chickens. [Method] Totally 500 individuals of 1-day-old Guangxi Partridge chickens were randomly divided into five groups, five replicates each group and 20 chicks each replicate. Groups A, B and C were treatment groups supplemented with 0.70%, 0.35% and0.175% P. felterrae extracts in the basal diet, respectively; group D was a medical control group supplemented with 0.01% colistin sulfate premix in the basal diet; and group E was a control group with basal diet. Serum samples were collected from 10 chickens in each group at 21, 35 and 49 days of age, to analyze the biochemical and immune indexes. [Result] It had no significant impact on serum TP and GLU contents of chickens by adding different levels of P. felterrae extracts in the basal diet（P 〉0.05）. However, at 21 and 35 days of age, the TBILI content of chickens in groups A, B and C were extremely lower or significantly lower than that in group E（ P〈0.01; P〈0.05）. At 35 days of age, the serum AKP activities in groups A, B and C were extremely higher than that in group E（P〈0.01）, and the serum GOT activities at 21 and 35 days of age in group C were significantly higher than that in group E（P〈0.05）. At 49 days of age, the serum Ig G content in groups A, B and C and the IL-2 content in group B and group C were significantly lower or extremely lower than that in group E（ P〈0.05; P〈0.01）; the T-AOC content in group B was significantly higher than that in group E（P〈0.05）; the GSH content in groups A, B and C were significantly higher than that in group E（ P〈0.05）. Adding different levels of P. felterrae extracts in the diet significantly decreased the TBILI content, reduced the Ig G, IL-2 and IL-6 contents, and improved the AKP and GOT activities and the T-AOC and GSH contents of Guangxi Partridge chicken. [Conclusion] Adding 0.35% P. felterrae extracts in the basal diet received the best effort.展开更多
采用PCR和直接测序的方法测定广西东兰乌鸡线粒体DNA(mt DNA)D-loop第一高变区序列,比较分析东兰乌鸡(片羽、丝羽)的遗传变异。结果表明:在东兰乌鸡mt DNA D-loop区中,共发现27个变异位点,46种单倍型,其变异方式主要是转换和缺失;检测...采用PCR和直接测序的方法测定广西东兰乌鸡线粒体DNA(mt DNA)D-loop第一高变区序列,比较分析东兰乌鸡(片羽、丝羽)的遗传变异。结果表明:在东兰乌鸡mt DNA D-loop区中,共发现27个变异位点,46种单倍型,其变异方式主要是转换和缺失;检测片段的G+C含量(60.59%)明显高于A+T含量(39.41%),具有偏倚性(P<0.05);单倍型多样度和核苷酸多样度分别为(0.890±0.020)、(0.01653±0.02483),表明东兰乌鸡的遗传多样性较丰富,其中丝羽乌鸡的变异位点、单倍型数量、单倍型多样度和核苷酸多样度等均比片羽乌鸡高。在NJ系统发生树上,东兰乌鸡分为3大支,说明具有3个血统来源,起源于中国红色原鸡。展开更多
基金Supported by the Special Project of Basic Scientific Research Business Expenses of Public Welfare Scientific Research Institutes Directly under Guangxi District(Gui Mu Yan Ke 2019-9)Guangxi Innovation Team Construction Project of National Modern Agricultural Industrial Technology System(nycytxgxcxtd).
文摘[Objectives]This trial was conducted to investigate the effects of diet with"Lianteng"additive of Chinese herbal medicine on blood biochemical indexes,immune and antioxidant function of Guangxi Sanhuang chickens.[Methods]480 Guangxi Sanhuang chickens at the age of 1 d were divided into 4 groups randomly and each group was assigned to 6 replicates with 20 chicks in each replicate;groups A,B and C were treatment groups and fed basal diets with 0.1%,0.2%and 0.4%of"Lianteng"additive of Chinese herbal medicine respectively during a trial of 70 d.Group D was control group and fed only basal diet.The serum samples were taken from 6 chicks in each group at the age of 21,35 and 49 d respectively and analyzed for biochemical,immune and antioxidant parameters in serum.[Results]Results from the trial showed that no difference was found in serum content of GLU,TG,TP,TC,and UN in the measurement at the age of 21,35 and 49 d(P>0.05);however,the content of ALB from groups A and B at 35 d was significantly higher than that from group D(P<0.01),and the ALB content from group B at 49 d was also higher than that from group D(P<0.05);the IgM content at 49 d from group A was lower than that from group D(P<0.05),in addition to this,there was no differences found in the contents of IgG,IgM and IFN-y among groups A,B,C and D during the trial(P>0.05);but the content of IL-2 from groups A and B at 49 d was significantly greater than that from group D(P<0.05);T-AOC from groups A,B and C at 49 d was very significantly higher than that from group D(P<0.01),and the one of group B at 35 d was still obviously higher than that of group D(P<0.05);also at 49 d,the GSH content in groups A and B was much greater than that in group D(P<0.01);T-SOD activity of group B and C at 35 d was distinctly greater than that of group D(P<0.05).[Conclusions]It can be concluded that the addition of 0.2%of"Lianteng"additive of Chinese herbal medicine in diet can increase immune function in a certain extent and promote the antioxidant ability observably for Guangxi Sanhuang chickens.
基金funded by the Program for Excellent Talents in Guangxi Higher Education Institutions (Guilin Education Talents 0857)the Fourth Project of the 11th Five-Year Plan for Guangxi New Century Education Reform Engineering(Guilin Higher Education[2008]No.178)
文摘[ Objective] To compare immune efficacy of avian influenza virus （AIV） Re-5 strain （HSN1 subtype） and SS strain （ H9 subtype） in Guangxi Sanhuang chickens. [Method] A total of 180 Sanhuang chickens were selected from a breeding chicken farm in Guilin City of Guangxi and assigned into four groups （ n = 45）. At 145 days old, the chickens in the group I and II were respectively inoculated with AIV Re-5 strain and AIV SS strain, but they were kept in cages separately. The two control groups were not inoculated. Antibody levels were determined at 170 days old. [ Result] The level of antibodies against AIV Re-5 strain was significantly higher in the chickens of the group I and group II than in the corresponding control groups, respectively （ P 〈 0.01 ）. The level of antibodies against AIV SS strain was not significantly different between the group I and its control group or between the group II and its control group. [ Conclusion] The antibody level of chickens can be increased by the AIV Re-5 strain but not by the AIV SS strain.
基金Supported by Science and Technology Development Program of Nanning City(20155181)Key Technology Research and Development Program of Guangxi Province(14121003-3-3)
文摘[Objective] This trial was to investigate the effect of dietary Picria felterrae Lour extracts on serum biochemical indexes and immune function of Guangxi Partridge chickens. [Method] Totally 500 individuals of 1-day-old Guangxi Partridge chickens were randomly divided into five groups, five replicates each group and 20 chicks each replicate. Groups A, B and C were treatment groups supplemented with 0.70%, 0.35% and0.175% P. felterrae extracts in the basal diet, respectively; group D was a medical control group supplemented with 0.01% colistin sulfate premix in the basal diet; and group E was a control group with basal diet. Serum samples were collected from 10 chickens in each group at 21, 35 and 49 days of age, to analyze the biochemical and immune indexes. [Result] It had no significant impact on serum TP and GLU contents of chickens by adding different levels of P. felterrae extracts in the basal diet（P 〉0.05）. However, at 21 and 35 days of age, the TBILI content of chickens in groups A, B and C were extremely lower or significantly lower than that in group E（ P〈0.01; P〈0.05）. At 35 days of age, the serum AKP activities in groups A, B and C were extremely higher than that in group E（P〈0.01）, and the serum GOT activities at 21 and 35 days of age in group C were significantly higher than that in group E（P〈0.05）. At 49 days of age, the serum Ig G content in groups A, B and C and the IL-2 content in group B and group C were significantly lower or extremely lower than that in group E（ P〈0.05; P〈0.01）; the T-AOC content in group B was significantly higher than that in group E（P〈0.05）; the GSH content in groups A, B and C were significantly higher than that in group E（ P〈0.05）. Adding different levels of P. felterrae extracts in the diet significantly decreased the TBILI content, reduced the Ig G, IL-2 and IL-6 contents, and improved the AKP and GOT activities and the T-AOC and GSH contents of Guangxi Partridge chicken. [Conclusion] Adding 0.35% P. felterrae extracts in the basal diet received the best effort.
文摘采用PCR和直接测序的方法测定广西东兰乌鸡线粒体DNA(mt DNA)D-loop第一高变区序列,比较分析东兰乌鸡(片羽、丝羽)的遗传变异。结果表明:在东兰乌鸡mt DNA D-loop区中,共发现27个变异位点,46种单倍型,其变异方式主要是转换和缺失;检测片段的G+C含量(60.59%)明显高于A+T含量(39.41%),具有偏倚性(P<0.05);单倍型多样度和核苷酸多样度分别为(0.890±0.020)、(0.01653±0.02483),表明东兰乌鸡的遗传多样性较丰富,其中丝羽乌鸡的变异位点、单倍型数量、单倍型多样度和核苷酸多样度等均比片羽乌鸡高。在NJ系统发生树上,东兰乌鸡分为3大支,说明具有3个血统来源,起源于中国红色原鸡。
