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Role of Progesterone in TLR4-MyD88-dependent Signaling Pathway in Pre-eclampsia
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作者 朱颖 吴敏 +1 位作者 吴超英 夏革清 《Journal of Huazhong University of Science and Technology(Medical Sciences)》 SCIE CAS 2013年第5期730-734,共5页
The role of progesterone in the Toll-like receptor 4 (TLR4)-MyD88-dependent signaling pathway in pre-eclampsia was studied. Peripheral blood mononuclear cells (PBMCs) from pre-eclampsia (PE) patients were subjec... The role of progesterone in the Toll-like receptor 4 (TLR4)-MyD88-dependent signaling pathway in pre-eclampsia was studied. Peripheral blood mononuclear cells (PBMCs) from pre-eclampsia (PE) patients were subjected to primary culture, and stimulated with different concentra- tions of progesterone (0, 10^-8, 10^-6, and 10^-4 mol/L). The mRNA expression of TLR4, MyD88 and nu- clear factor-kappaB (NF-κB) was detected by using real-time PCR. The Ikappa-B protein expression was detected by using Western blotting. The expression of tumor necrosis factor-or (TNF-α and inter- leukin-6 (IL-6) in the supernatant was determined by using ELISA. With the concentrations of proges- terone increasing, the mRNA expression levels of TLR4, MyD88 and NF-κB in 2^△△CT value were sig- nificantly decreased, and the IkappaB protein expression levels were significantly increased. The TNF-α and IL-6 expression showed a downward trend when the progesterone concentration increased, and there were significant differences among all of the groups (P〈0.05). It was suggested that progesterone can inhibit the TLR4-MyD88-dependent signaling pathway in PE significantly and benefit for the preg- nancy. 展开更多
关键词 PROGESTERONE PRE-ECLAMPSIA Toll-like receptor 4 tumor necrosis factor-α nuclear tac-tor-b ikappa-κb INTERLEUKIN-6
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