Using polyvinyl alcohol (PVA) as raw material and vacuum pump oil as oil phase medium, PVA-based microcarriers were prepared by suspension method. The diameters of the beads were 100-180μm. LO2 cells were cultured on...Using polyvinyl alcohol (PVA) as raw material and vacuum pump oil as oil phase medium, PVA-based microcarriers were prepared by suspension method. The diameters of the beads were 100-180μm. LO2 cells were cultured on PVA-based microcarriers and cytodexIII microcarriers. Morphology, attachment and growth rate of LO2 cells were studied.展开更多
To address the controversial issue of the toxicity of dental alloys and silver nanoparticles in medical ap- plications, an in vivo-like LO2 3-D model was constructed within polyvinylidene fluoride hollow fiber materia...To address the controversial issue of the toxicity of dental alloys and silver nanoparticles in medical ap- plications, an in vivo-like LO2 3-D model was constructed within polyvinylidene fluoride hollow fiber materials to mimic the microenvironment of liver tissue. The use of microscopy methods and the measurement of liver-specific functions optimized the model for best cell performances and also proved the superiority of the 3-D LO2 model when compared with the traditional monolayer model. Toxicity tests were conducted using the newly constructed model, finding that four dental castings coated with silver nanoparticles were toxic to human hepatocytes after cell viability assays. In general, the toxicity of both the castings and the coated silver nanoparticles aggravated as time increased, yet the nanoparticles attenuated the general toxicity by preventing metal ion release, especially at high concentrations.展开更多
西凤酒中微量成分种类丰富,但关于其微量成分的作用及量效关系研究较少。因此,该研究基于细胞和动物实验探讨了西凤酒(以红西凤为代表)中微量成分对LO2细胞和健康小鼠肠道菌群的影响情况。细胞实验结果表明,乙醇浓度越高,细胞增殖抑制...西凤酒中微量成分种类丰富,但关于其微量成分的作用及量效关系研究较少。因此,该研究基于细胞和动物实验探讨了西凤酒(以红西凤为代表)中微量成分对LO2细胞和健康小鼠肠道菌群的影响情况。细胞实验结果表明,乙醇浓度越高,细胞增殖抑制率、谷丙转氨酶(alanine aminotransferase,ALT)和谷草转氨酶(aspartate aminotransferase,AST)泄漏量越大,相同乙醇浓度下,红西凤和食用酒精(对照组)干预组之间具有显著性差异(P<0.05)。动物实验结果表明,不同剂量红西凤和食用酒精干预均导致小鼠体重、高密度脂蛋白胆固醇(high density lipoprotein-cholesterol,HDL-C)含量降低,肝重体重比、AST、ALT、TC、总胆固醇(total cholesterol,TC)、总甘油三酯(total triglycerides,TG)、低密度脂蛋白胆固醇(low density lipoprotein-cholesterol,LDL-C)含量升高,肝脏组织出现不同程度脂肪变性,肠道菌群发生明显变化(门水平上,厚壁菌门与拟杆菌门比例下降;属水平上,乳酸杆菌属、毛螺菌属等相对丰度降低,幽门螺杆菌属、链球菌属等相对丰度增加),LEfSe分析结果显示,小鼠肠道菌群存在的差异与红西凤和食用酒精干预剂量有一定关系,且高剂量干预对小鼠肠道菌群的影响有扩大的趋势,然而同剂量下,红西凤干预对小鼠的损伤程度轻于食用酒精,以低剂量红西凤干预对小鼠的影响最轻,推测红西凤中各微量成分可能通过调节小鼠肠道菌群从而减缓对肝脏的损伤程度。以上结果说明,适量摄入红西凤在一定程度上可以缓解酒精对LO2细胞及小鼠肠道菌群的负面影响,该研究将为西凤酒的健康消费提供理论依据。展开更多
目的:探讨建立人肝细胞系LO2单纯肝脂肪变性细胞模型的方法。方法:试验分为正常对照组与试验组,用含10%胎牛血清的1640培养基培养LO2细胞,当细胞处于对数生长期时,正常对照组更换新鲜的1640培养基,试验组用含不同浓度游离脂肪酸(油酸∶...目的:探讨建立人肝细胞系LO2单纯肝脂肪变性细胞模型的方法。方法:试验分为正常对照组与试验组,用含10%胎牛血清的1640培养基培养LO2细胞,当细胞处于对数生长期时,正常对照组更换新鲜的1640培养基,试验组用含不同浓度游离脂肪酸(油酸∶棕榈酸为2∶1)的1640培养基培养,24 h后油红O染色,观察细胞内脂滴大小,并检测细胞中TG、TC含量的变化及细胞增殖情况。结果:试验组中LO2细胞内有大量的脂滴生成,且甘油三酯水平显著升高,其中,以1.2 mM FFA组脂质沉积最为明显。同时,细胞抑制率小于4%,胆固醇水平无明显升高,均符合单纯脂肪变性模型的特征。结论:采用游离脂肪酸(油酸∶棕榈酸为2∶1)可以成功诱导LO2细胞单纯脂肪变性,其中游离脂肪酸浓度以1.2 m M最为理想。展开更多
基金The National Natural Science Foundation of China(No.29776027).
文摘Using polyvinyl alcohol (PVA) as raw material and vacuum pump oil as oil phase medium, PVA-based microcarriers were prepared by suspension method. The diameters of the beads were 100-180μm. LO2 cells were cultured on PVA-based microcarriers and cytodexIII microcarriers. Morphology, attachment and growth rate of LO2 cells were studied.
基金Project supported by the Zhejiang Provincial Natural Science Foundation of China(No.LZ14C200001)the Public Welfare Project of Science Technology Department of Zhejiang Province(No.2013c33139),China
文摘To address the controversial issue of the toxicity of dental alloys and silver nanoparticles in medical ap- plications, an in vivo-like LO2 3-D model was constructed within polyvinylidene fluoride hollow fiber materials to mimic the microenvironment of liver tissue. The use of microscopy methods and the measurement of liver-specific functions optimized the model for best cell performances and also proved the superiority of the 3-D LO2 model when compared with the traditional monolayer model. Toxicity tests were conducted using the newly constructed model, finding that four dental castings coated with silver nanoparticles were toxic to human hepatocytes after cell viability assays. In general, the toxicity of both the castings and the coated silver nanoparticles aggravated as time increased, yet the nanoparticles attenuated the general toxicity by preventing metal ion release, especially at high concentrations.
文摘西凤酒中微量成分种类丰富,但关于其微量成分的作用及量效关系研究较少。因此,该研究基于细胞和动物实验探讨了西凤酒(以红西凤为代表)中微量成分对LO2细胞和健康小鼠肠道菌群的影响情况。细胞实验结果表明,乙醇浓度越高,细胞增殖抑制率、谷丙转氨酶(alanine aminotransferase,ALT)和谷草转氨酶(aspartate aminotransferase,AST)泄漏量越大,相同乙醇浓度下,红西凤和食用酒精(对照组)干预组之间具有显著性差异(P<0.05)。动物实验结果表明,不同剂量红西凤和食用酒精干预均导致小鼠体重、高密度脂蛋白胆固醇(high density lipoprotein-cholesterol,HDL-C)含量降低,肝重体重比、AST、ALT、TC、总胆固醇(total cholesterol,TC)、总甘油三酯(total triglycerides,TG)、低密度脂蛋白胆固醇(low density lipoprotein-cholesterol,LDL-C)含量升高,肝脏组织出现不同程度脂肪变性,肠道菌群发生明显变化(门水平上,厚壁菌门与拟杆菌门比例下降;属水平上,乳酸杆菌属、毛螺菌属等相对丰度降低,幽门螺杆菌属、链球菌属等相对丰度增加),LEfSe分析结果显示,小鼠肠道菌群存在的差异与红西凤和食用酒精干预剂量有一定关系,且高剂量干预对小鼠肠道菌群的影响有扩大的趋势,然而同剂量下,红西凤干预对小鼠的损伤程度轻于食用酒精,以低剂量红西凤干预对小鼠的影响最轻,推测红西凤中各微量成分可能通过调节小鼠肠道菌群从而减缓对肝脏的损伤程度。以上结果说明,适量摄入红西凤在一定程度上可以缓解酒精对LO2细胞及小鼠肠道菌群的负面影响,该研究将为西凤酒的健康消费提供理论依据。
文摘目的:探讨建立人肝细胞系LO2单纯肝脂肪变性细胞模型的方法。方法:试验分为正常对照组与试验组,用含10%胎牛血清的1640培养基培养LO2细胞,当细胞处于对数生长期时,正常对照组更换新鲜的1640培养基,试验组用含不同浓度游离脂肪酸(油酸∶棕榈酸为2∶1)的1640培养基培养,24 h后油红O染色,观察细胞内脂滴大小,并检测细胞中TG、TC含量的变化及细胞增殖情况。结果:试验组中LO2细胞内有大量的脂滴生成,且甘油三酯水平显著升高,其中,以1.2 mM FFA组脂质沉积最为明显。同时,细胞抑制率小于4%,胆固醇水平无明显升高,均符合单纯脂肪变性模型的特征。结论:采用游离脂肪酸(油酸∶棕榈酸为2∶1)可以成功诱导LO2细胞单纯脂肪变性,其中游离脂肪酸浓度以1.2 m M最为理想。
