[Objectives] To systematically identify and analyze pharmacognostical features of Mongolian medicine Shoushen and Naishoushen( dairy tablets) to provide scientific basis for the establishment and identification of its...[Objectives] To systematically identify and analyze pharmacognostical features of Mongolian medicine Shoushen and Naishoushen( dairy tablets) to provide scientific basis for the establishment and identification of its quality standard. [Methods] According to relevant methods specified in Appendix to 2010 Chinese Pharmacopoeia,the water content,total ash,and extracts of Shoushen and Naishoushen were detected,and thin layer chromatography( TLC) was applied to make qualitative identification. Gastrodin was used as the reference substance,extracted with 70% methanol,and then sprayed with ethyl acetate-formic acid-water( 9∶ 1∶ 0. 2) as the developing solvent,and then sprayed with 10% phosphomolybdic acid ethanol solution and heated at 105℃ to clear spot color. UV-Visible spectrophotometry and high performance liquid chromatography( HPLC) were used to analyze the content of polysaccharides and gastrodins in Shoushen and Naishoushen. The chromatographic column was a ZOBB AX eclipse XDB-C_(18)( 4. 6 mm × 250 mm,5 cm) column with the methanol-0. 04% phosphoric acid solution( 8∶92) as the mobile phase,the flow rate of 1. 0mL/min,the detection wavelength of 222 nm,and the column temperature of 30℃.[Results]In the thin-layer chromatography test,spots of the same color appeared at the positions corresponding to the chromatogram of the reference substance. Gastrodin eluted under high pressure reached baseline separation. Gastrodin had a good linearity in the concentration range of 0. 009-0. 09 mg/m L. The regression equation was Y = 586 866 X + 425 821( R^2= 0. 999 6),and the average recovery rate was100. 1%. Precision test,reproducibility test,and stability test conformed to the requirements. The results of extracts of three batches of samples were 26. 13%-42. 58%,water content was 3. 47%-5. 31%,and total ash was 5. 43%-6. 33%. [Conclusions] The quality control method has high reliability,high sensitivity,high specificity,high accuracy,and high stability. The results are expected to provide a scientific basis for the identification,resource utilization,and improvement of quality standard for Shoushen and Naishoushen.展开更多
用1951—2008年58 a 1月10日—2月2日1 000 hPa高度场逐日NCEP/NCAR再分析格点资料,求得逐日蒙古高压的强度P和中心位置λc、c指数,用其对"0801南方雪灾"期间蒙古高压的中期演变过程进行统计分析。结果表明,2008年1月10日—...用1951—2008年58 a 1月10日—2月2日1 000 hPa高度场逐日NCEP/NCAR再分析格点资料,求得逐日蒙古高压的强度P和中心位置λc、c指数,用其对"0801南方雪灾"期间蒙古高压的中期演变过程进行统计分析。结果表明,2008年1月10日—2月2日蒙古高压强度P和中心所在纬度φc出现了连续4次振荡,它们与我国南方降温、降水振荡过程准同步。由P、(λc,c)给出的综合动态图上蒙古高压4次活动过程也很明显地与降温、降水中期过程一一对应。分析表明2008年1月10日—2月2日蒙古高压的这一中期演变特征,是1951年以来仅有的一次。因此,可以认为,在充沛水汽供应条件下蒙古高压强而连续的爆发是导致"0801南方雪灾"产生的直接环流成因。展开更多
目的采用高效液相色谱法建立蒙药材玉簪花指纹图谱分析方法 ,为玉簪花药材的质量评价提供参考依据。方法采用高效液相色谱法对蒙药材玉簪花指纹图谱进行研究,色谱柱为Phenomenex Luna C_(18)(250 mm×4.6 mm,5μm),以甲醇-0.5%磷酸...目的采用高效液相色谱法建立蒙药材玉簪花指纹图谱分析方法 ,为玉簪花药材的质量评价提供参考依据。方法采用高效液相色谱法对蒙药材玉簪花指纹图谱进行研究,色谱柱为Phenomenex Luna C_(18)(250 mm×4.6 mm,5μm),以甲醇-0.5%磷酸水溶液为流动相,梯度洗脱,流速为1.0 m L/min;柱温为30℃;检测波长256 nm,结果采用"中药色谱指纹图谱相似度评价系统"以中位数法生成标准指纹图谱。结果 10个批次的玉簪花药材的色谱指纹图谱有18个共有峰,高效液相色谱指纹图谱的相似度都>0.9,建立共有模式,不同批次玉簪花指纹图谱相似性较好。结论高效液相色谱法用于蒙药玉簪花指纹图谱研究可为玉簪花药材质量评价和今后规范药用资源提供科学依据。展开更多
基金Supported by Inner Mongolia Science and Technology Foundation Project(kjt15sf22)
文摘[Objectives] To systematically identify and analyze pharmacognostical features of Mongolian medicine Shoushen and Naishoushen( dairy tablets) to provide scientific basis for the establishment and identification of its quality standard. [Methods] According to relevant methods specified in Appendix to 2010 Chinese Pharmacopoeia,the water content,total ash,and extracts of Shoushen and Naishoushen were detected,and thin layer chromatography( TLC) was applied to make qualitative identification. Gastrodin was used as the reference substance,extracted with 70% methanol,and then sprayed with ethyl acetate-formic acid-water( 9∶ 1∶ 0. 2) as the developing solvent,and then sprayed with 10% phosphomolybdic acid ethanol solution and heated at 105℃ to clear spot color. UV-Visible spectrophotometry and high performance liquid chromatography( HPLC) were used to analyze the content of polysaccharides and gastrodins in Shoushen and Naishoushen. The chromatographic column was a ZOBB AX eclipse XDB-C_(18)( 4. 6 mm × 250 mm,5 cm) column with the methanol-0. 04% phosphoric acid solution( 8∶92) as the mobile phase,the flow rate of 1. 0mL/min,the detection wavelength of 222 nm,and the column temperature of 30℃.[Results]In the thin-layer chromatography test,spots of the same color appeared at the positions corresponding to the chromatogram of the reference substance. Gastrodin eluted under high pressure reached baseline separation. Gastrodin had a good linearity in the concentration range of 0. 009-0. 09 mg/m L. The regression equation was Y = 586 866 X + 425 821( R^2= 0. 999 6),and the average recovery rate was100. 1%. Precision test,reproducibility test,and stability test conformed to the requirements. The results of extracts of three batches of samples were 26. 13%-42. 58%,water content was 3. 47%-5. 31%,and total ash was 5. 43%-6. 33%. [Conclusions] The quality control method has high reliability,high sensitivity,high specificity,high accuracy,and high stability. The results are expected to provide a scientific basis for the identification,resource utilization,and improvement of quality standard for Shoushen and Naishoushen.
文摘用1951—2008年58 a 1月10日—2月2日1 000 hPa高度场逐日NCEP/NCAR再分析格点资料,求得逐日蒙古高压的强度P和中心位置λc、c指数,用其对"0801南方雪灾"期间蒙古高压的中期演变过程进行统计分析。结果表明,2008年1月10日—2月2日蒙古高压强度P和中心所在纬度φc出现了连续4次振荡,它们与我国南方降温、降水振荡过程准同步。由P、(λc,c)给出的综合动态图上蒙古高压4次活动过程也很明显地与降温、降水中期过程一一对应。分析表明2008年1月10日—2月2日蒙古高压的这一中期演变特征,是1951年以来仅有的一次。因此,可以认为,在充沛水汽供应条件下蒙古高压强而连续的爆发是导致"0801南方雪灾"产生的直接环流成因。
文摘目的采用高效液相色谱法建立蒙药材玉簪花指纹图谱分析方法 ,为玉簪花药材的质量评价提供参考依据。方法采用高效液相色谱法对蒙药材玉簪花指纹图谱进行研究,色谱柱为Phenomenex Luna C_(18)(250 mm×4.6 mm,5μm),以甲醇-0.5%磷酸水溶液为流动相,梯度洗脱,流速为1.0 m L/min;柱温为30℃;检测波长256 nm,结果采用"中药色谱指纹图谱相似度评价系统"以中位数法生成标准指纹图谱。结果 10个批次的玉簪花药材的色谱指纹图谱有18个共有峰,高效液相色谱指纹图谱的相似度都>0.9,建立共有模式,不同批次玉簪花指纹图谱相似性较好。结论高效液相色谱法用于蒙药玉簪花指纹图谱研究可为玉簪花药材质量评价和今后规范药用资源提供科学依据。
