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EDC/NHS交联重组胶原蛋白水凝胶修复关节软骨缺损 被引量:1
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作者 黄长瑾 雷桓 唐晓军 《中国材料进展》 CAS CSCD 北大核心 2024年第4期344-354,共11页
关节软骨损伤、缺损是一种常见且严峻的临床挑战,没有有效的治疗方法,基于胶原蛋白的水凝胶是软骨组织工程的研究热点之一,但在开发具有良好生物相容性且可安全交联的胶原蛋白产品,并提高软骨修复有效性方面仍然存在巨大挑战。将重组Ⅰ... 关节软骨损伤、缺损是一种常见且严峻的临床挑战,没有有效的治疗方法,基于胶原蛋白的水凝胶是软骨组织工程的研究热点之一,但在开发具有良好生物相容性且可安全交联的胶原蛋白产品,并提高软骨修复有效性方面仍然存在巨大挑战。将重组Ⅰ型胶原蛋白和重组Ⅲ型胶原蛋白通过EDC/NHS交联,制备了2种水凝胶,均表现出良好的三维孔隙结构以及优异的力学性能和可降解性。研究发现,重组Ⅰ型胶原蛋白水凝胶(Gel-Ⅰ)和重组Ⅲ型胶原蛋白水凝胶(Gel-Ⅲ)能促进人骨髓间充质干细胞(human bone-marrow mesenchymal stem cells,HBMSCs)糖胺聚糖的分泌,Gel-Ⅰ可以显著上调HBMSCs表达COLⅡ基因和SOX9基因,Gel-Ⅲ可以显著上调SOX9基因表达。在兔子膝关节软骨修复模型中,与对照组和Gel-Ⅲ组相比,Gel-Ⅰ组在骨体积大小方面表现出明显优势,软骨下骨已形成并有效恢复,小梁的数量和厚度最高,小梁分布更均匀,并且缺损处新软骨厚度与相邻软骨厚度相同。因此,Gel-Ⅰ在软骨修复有效性以及安全性方面具有极大的临床应用潜力。 展开更多
关键词 EDC/nhs 交联 重组胶原蛋白 可注射水凝胶 关节软骨修复
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英国NHS的组织架构改革及其对中国医疗资源整合的启示
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作者 周文 《卫生经济研究》 北大核心 2024年第6期64-67,共4页
2022年,英国国家医疗服务体系(NHS)经历了一次改革,建立了42个综合护理系统(ICS),取代此前211个临床医疗服务委托委员会(CCG),这是2012年以来NHS首次大规模的组织架构改革。ICS相比于CCG而言,融入了更加丰富的社会护理服务,注重发挥区... 2022年,英国国家医疗服务体系(NHS)经历了一次改革,建立了42个综合护理系统(ICS),取代此前211个临床医疗服务委托委员会(CCG),这是2012年以来NHS首次大规模的组织架构改革。ICS相比于CCG而言,融入了更加丰富的社会护理服务,注重发挥区域内的规模化效益,并致力于构建人群健康管理体系。这个改革对中国的医疗资源整合有两点启示,一是鼓励基层医疗卫生机构开展线上诊疗,二是促进开展家庭医生签约服务。 展开更多
关键词 英国国家医疗服务体系 组织架构改革 医疗资源整合 启示
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A Study on Inflation Theories and Turkey’s Inflation Problem
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作者 SıtkıSelim Dolanay 《Management Studies》 2024年第5期309-322,共14页
In the Turkish Economy,with the policies of opening up to the outside world and transition to a free market economy in the 1980s,there have been radical changes in the structure of the economy and the prices of all go... In the Turkish Economy,with the policies of opening up to the outside world and transition to a free market economy in the 1980s,there have been radical changes in the structure of the economy and the prices of all goods and services have begun to be determined by the market under market conditions.In the 1980s,which can be called the transition process to a free market economy and open economy,on the one hand,efforts were made to privatize state institutions in order to withdraw the state from economic life and to ensure that the prices of goods and services were determined under free market conditions,while on the other hand,efforts were made to grant freedom to foreign trade.While these two main issues were being realized,the import substitution industrialization strategy was abandoned and an export-based growth model was started to be implemented.The last step of this opening up and liberalization process was realized with the decision numbered 32 in 1989.With the liberalization of capital movements in the 1990s,we can say that economic growth and development was attempted to be achieved through hot money inflows rather than direct foreign investments.This orientation made the economy more vulnerable to crises,and for the first time,a crisis occurred in the form of the 1994 economic crisis,which was understood to be caused by hot money.The 1994 economic crisis was attempted to be overcome by providing state guarantees for bank deposits and applying high interest rates.Thus,it is noteworthy that high inflation was experienced in the period leading up to the 2001 economic crisis.Indeed,while the increase in the wholesale price index rarely remained below 30%on an annual basis between 1980 and 1990,increases of 50%and 60%began to be seen.However,the period between 1990 and 2000 was a period in which higher inflation rates were encountered.As a result of the environment created in the 1990s,we can say that the financial crisis experienced in 2001 deepened into an economic crisis and that this crisis had economic and political effects.Although there have been many economic crises in the history of the Republic,this crisis is considered to be the most deeply effective.While the crisis was overcome with the stand-by agreement made by the IMF and the announced Transition to a Strong Economy program,the economy began to grow rapidly with the ease of use of foreign resources,and the existence of political stability seems to have ensured that this economic growth process continued uninterruptedly except for 2009.A decrease was also experienced in inflation rates.After 2015,inflation rates began to rise again,and although negative economic growth rates were experienced between the 3rd quarter of 2018 and the 3rd quarter of 2019 during the exchange rate shock period in 2018,we can say that this was not effective enough to turn annual average growth rates into negatives.The exchange rate shock experienced in November 2021 both caused changes in economic policies and the adoption of new policies,and seems to have led to an economic crisis wave caused by the large increase in inflation rates.Within this framework,a post-Keynesian policy of combating inflation has been implemented,and with the new economic policies announced in June 2023,the Central Bank has gradually increased policy interest rates and started to implement tight monetary policy in cooperation with the Ministry of Treasury and Finance,and we can say that a new phase has been entered in the post-Keynesian policy of combating inflation.However,in our opinion,it is necessary to determine well where inflation originates from and what ensures its continuation.In this context,Turkey’s inflation problem will be analyzed by utilizing the views of post-Keynesians on inflation and the profit-push inflation approach. 展开更多
关键词 Turkish economy free market economy export-based growth model inflation theories post-Keynesian approach to inflation profit-push inflation approach
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NHS签出大额IT合同背后
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作者 黄柳 《中国医院院长》 2024年第5期18-19,共2页
“统一的数据库可能会改变传统医疗运行的规则,NHS签出的这份新合同是一个很好的开始。”英国国家医疗服务体系(NHS)有史以来最大额度的信息系统升级合同花落一家美国公司。1英镑为NHS在疫情中排忧解难,随后加速嵌入N H S信息系统业务... “统一的数据库可能会改变传统医疗运行的规则,NHS签出的这份新合同是一个很好的开始。”英国国家医疗服务体系(NHS)有史以来最大额度的信息系统升级合同花落一家美国公司。1英镑为NHS在疫情中排忧解难,随后加速嵌入N H S信息系统业务的智能科技公司Palantir在2023年底赢得了这份合同。合同的金额一度被媒体报道为超过4亿英镑,但最后政府在11月正式宣布的额度为3.3亿英镑,据称额外资金被预留用于夯实隐私功能,以及购置其他优化类产品。 展开更多
关键词 智能科技 信息系统 数据库 媒体报道 合同 IT nhs 美国公司
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英国国家卫生服务体系(NHS)绩效评估及其实践评价 被引量:6
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作者 张国玉 《标准科学》 CSSCI 2009年第4期24-27,共4页
介绍了英国国家卫生服务体系(NHS)绩效评估的实施背景及绩效评估框架(PAF)和绩效示标体系,并对其在实践中的实施效果和局限进行了评价,以期对中国医疗机构绩效评估提供经验借鉴。
关键词 英国国家卫生服务体系(nhs) 绩效评估框架(PAF) nhs绩效示标
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Similarities and Differences of Chinese Translation Theories And Western Translation Theories 被引量:14
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作者 黄雯怡 《海外英语》 2017年第2期241-244,共4页
Translation theories are based on the different languages and cultures.The difference is not clearly defined,so there is a place of mutual integration that can be used interchangeably.Theyall regard author’s meaning ... Translation theories are based on the different languages and cultures.The difference is not clearly defined,so there is a place of mutual integration that can be used interchangeably.Theyall regard author’s meaning and style as a core to translate a good product.Comparatively,Chinese theories focus on expressing the general meaning of the author,but Western theory emphasizes equivalence of details on translation and response of reader.In the translation practice,according to different theoretical texts and different stages of the translation process,the Chinese and Western translation theories are combined to apply them for making up for each other's deficiencies,which can help our translators to solve the problems in the process of translation improve the construction of Chinese theories. 展开更多
关键词 chinese translation theories western translation theories SIMILARITIES DIFFERENCES
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Special Relativity’s “Newtonization” in Complex “Para-Space”: The Two Theories Equivalence Question
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作者 Jerzy K. Filus 《Journal of Applied Mathematics and Physics》 2024年第7期2421-2451,共31页
Complex model, say C3, of “para-space” as alternative to the real M4 Minkowski space-time for both relativistic and classical mechanics was shortly introduced as reference to our previous works on that subject. The ... Complex model, say C3, of “para-space” as alternative to the real M4 Minkowski space-time for both relativistic and classical mechanics was shortly introduced as reference to our previous works on that subject. The actual aim, however, is an additional analysis of the physical and para-physical phenomena’ behavior as we formally transport observable mechanical phenomena [motion] to non-real interior of the complex domain. As it turns out, such procedure, when properly set, corresponds to transition from relativistic to more classic (or, possibly, just classic) kind of the motion. This procedure, we call the “Newtonization of relativistic physical quantities and phenomena”, first of all, includes the mechanical motion’s characteristics in the C3. The algebraic structure of vector spaces was imposed and analyzed on both: the set of all relativistic velocities and on the set of the corresponding to them “Galilean” velocities. The key point of the analysis is realization that, as a matter of fact, the relativistic theory and the classical are equivalent at least as for the kinematics. This conclusion follows the fact that the two defined structures of topological vector spaces i.e., the structure imposed on sets of all relativistic velocities and the structure on set of all “Galilean” velocities, are both diffeomorphic in their topological parts and are isomorphic as the vector spaces. As for the relativistic theory, the two approaches: the hyperbolic (“classical” SR) with its four-vector formalism and Euclidean, where SR is modeled by the complex para-space C3, were analyzed and compared. 展开更多
关键词 Special Relativity’s Hyperbolic Versus Circular Versions Galilean Kinematics Partial Equivalence of SR and Newton’s theories Algebra of Relativistic and the Corresponding Galilean Velocities
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NHS将扩大向私营部门购买服务
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作者 曹凯 《中国医院院长》 2024年第20期20-21,共2页
就医等待时间过长,大量需求无法被满足,英国私营医疗机构持续扩大服务供给。时间临近2024年末,英国国会开始审查2025财年(2025年4月至2026年3月)的财政预算,重新上台执政的工党如何落地其卫生政策承诺受到社会关注。一直以来,医疗服务... 就医等待时间过长,大量需求无法被满足,英国私营医疗机构持续扩大服务供给。时间临近2024年末,英国国会开始审查2025财年(2025年4月至2026年3月)的财政预算,重新上台执政的工党如何落地其卫生政策承诺受到社会关注。一直以来,医疗服务等待时间过长的问题都困扰英国国家医疗服务体系(National Health Service,NHS)。就在刚刚过去的竞选过程中,英国两党就服务等待时间过长的问题进行激烈舆论攻防,选举过后也一直是社会关注的焦点。 展开更多
关键词 财政预算 卫生政策 购买服务 服务供给 私营部门 英国国会 nhs
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Chinese Education Modernization:A Case Study on Ideological and Political Theories Teaching in All Courses with Organic Integration of Cultural Literacy and Political Education
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作者 Zongyu Gan 《Journal of Contemporary Educational Research》 2024年第5期172-176,共5页
Chinese education modernization is integral to Chinese modernization,and is of leading significance to the further advancement of Chinese modernization and the great rejuvenation of the Chinese nation.In this paper,ta... Chinese education modernization is integral to Chinese modernization,and is of leading significance to the further advancement of Chinese modernization and the great rejuvenation of the Chinese nation.In this paper,taking ideological and political theories teaching in all courses as an example,an attempt is made to organically integrate cultural literacy and political education under the vision of Chinese education modernization,and“gathers knowledge heritage in the dissemination of value and leads the values in the dissemination of knowledge,”thereby training students into highcaliber talents with good and all-round moral,intellectual,physical,aesthetical,and labor grounding. 展开更多
关键词 Chinese education modernization Ideological and political theories teaching in all courses EDUCATION
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英国NHS体系对我国卫生服务的启示 被引量:17
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作者 张嵬 马玉琴 +1 位作者 段光锋 李婷 《解放军医院管理杂志》 2012年第6期599-600,共2页
本文介绍英国NHS体系的特点,包括公平优先、兼顾效率,合理的分级保健体制,健全的医药体制及电子化健康服务,对我国卫生服务启示:动员社会资金投入卫生服务,推行家庭医师制度,推进药物制度建设,加强卫生信息化建设。
关键词 特点 卫生服务 nhs
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英国NHS改革对我国的启示 被引量:16
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作者 詹国彬 王雁红 《南京社会科学》 CSSCI 北大核心 2010年第9期36-42,63,共8页
回望近30年的改革历程,以内部市场导向的NHS改革在提升医疗服务效率、增强医疗机构回应性以及控制医疗费用增长等方面取得了较好的成效。本文旨在考察和分析英国NHS改革的现实动因与路径选择,以期获得对英国NHS改革的理性认识及其对我... 回望近30年的改革历程,以内部市场导向的NHS改革在提升医疗服务效率、增强医疗机构回应性以及控制医疗费用增长等方面取得了较好的成效。本文旨在考察和分析英国NHS改革的现实动因与路径选择,以期获得对英国NHS改革的理性认识及其对我国医疗卫生体制改革的有益启示。 展开更多
关键词 英国 nhs 内部市场 路径选择
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用于固相放射免疫分析的一类聚合物NHS酯的合成与表征 被引量:4
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作者 胡子文 谢家理 +2 位作者 王科太 冯立 郝艳静 《四川大学学报(自然科学版)》 CAS CSCD 北大核心 2003年第2期310-314,共5页
将NHS(N 羟基琥珀酰亚胺 )酯功能基团引入高分子载体上 ,合成了几种聚合物的NHS酯 .研究了合成单体NHS酯及聚合物NHS酯的合成条件 ,测试了NHS酯及聚合物NHS酯的基本特性 .该聚合物NHS酯可在温和条件下与蛋白反应 ,使蛋白以共价键结合方... 将NHS(N 羟基琥珀酰亚胺 )酯功能基团引入高分子载体上 ,合成了几种聚合物的NHS酯 .研究了合成单体NHS酯及聚合物NHS酯的合成条件 ,测试了NHS酯及聚合物NHS酯的基本特性 .该聚合物NHS酯可在温和条件下与蛋白反应 ,使蛋白以共价键结合方式固定在聚合物上 。 展开更多
关键词 固相放射免疫分析 聚合物nhs 合成 共价结合 N—羟基琥珀酰亚胺 医学检验
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EDC和NHS对玉米醇溶蛋白成膜性质的影响 被引量:6
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作者 张敏 陈野 +3 位作者 刘君 周淑红 王君予 李燕丹 《食品科学》 EI CAS CSCD 北大核心 2014年第1期12-17,共6页
为提高玉米醇溶蛋白膜的抗拉强度和伸长率,降低其水蒸气透过率和吸水率,研究交联剂1-乙基-3-(3-二甲基氨丙基)碳二亚胺盐酸盐(EDC)和N-羟基琥珀酰亚胺(NHS)对成膜性质的影响。结果表明:以90%乙醇为溶剂,EDC和NHS的添加量分别为0.06 g/g... 为提高玉米醇溶蛋白膜的抗拉强度和伸长率,降低其水蒸气透过率和吸水率,研究交联剂1-乙基-3-(3-二甲基氨丙基)碳二亚胺盐酸盐(EDC)和N-羟基琥珀酰亚胺(NHS)对成膜性质的影响。结果表明:以90%乙醇为溶剂,EDC和NHS的添加量分别为0.06 g/g时,制得的膜性能最佳,抗拉强度为83 MPa,较未添加交联剂的蛋白膜提高97.6%;伸长率为5.5%,提高57.1%;水蒸气透过率为2.5×10-8(g·m)/(m2·h·Pa),降低43.2%;吸水率为39.4%,降低24.4%;质量损失率为3.6%,增加20.0%;静态接触角为67.4°,表明膜表面仍为疏水表面。原子力显微镜观测显示,加入交联剂后,蛋白分子聚集体变小,以均一的小球型聚集体形式紧密有序排列。 展开更多
关键词 1-乙基-3-(3-二甲基氨丙基)碳二亚胺盐酸盐(EDC) N-羟基琥珀酰亚胺(nhs) 玉米醇溶蛋白 成膜性质
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EDC/NHS交联胶原基牙周组织引导再生膜材料的研究 被引量:1
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作者 季培红 侯思润 +2 位作者 林绣丹 任力 刘卅 《广东工业大学学报》 CAS 2013年第3期122-126,共5页
采用胶原材料制备了一种密实-疏松双层结构的牙周引导再生膜材料.为改善再生膜材料的物理化学性能,采用1-乙基-3-(3-二甲基氨丙基)碳二亚胺(EDC)和N-羟基琥珀酰亚胺(NHS)为交联剂对膜材料进行交联,考察了不同交联剂质量浓度对膜材料物... 采用胶原材料制备了一种密实-疏松双层结构的牙周引导再生膜材料.为改善再生膜材料的物理化学性能,采用1-乙基-3-(3-二甲基氨丙基)碳二亚胺(EDC)和N-羟基琥珀酰亚胺(NHS)为交联剂对膜材料进行交联,考察了不同交联剂质量浓度对膜材料物理化学性能的影响,并通过差示扫描量热分析仪(DSC)、扫描电镜(SEM)、吸水率测试、膨胀动力学分析、抗酶解性能分析等手段对膜材料交联前后的结构与性能进行了研究.结果表明,以EDC/NHS为交联剂,在pH为5.5、EDC质量浓度为5 g/L、交联时间为24 h的条件下,引导再生胶原膜材料的综合性能达到最佳,进一步提高交联剂的浓度,材料物理化学性能的变化并不明显.采用EDC/NHS交联后,可显著改善再生膜材料的物理化学性能.交联后膜材料的变性温度和抗酶解性能显著提高,并且维持了密实-疏松的双层结构. 展开更多
关键词 组织引导再生膜 胶原 交联 EDC nhs
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The Fate of Supersymmetry in Quantum Field Theories
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作者 Risto Raitio 《Journal of High Energy Physics, Gravitation and Cosmology》 CAS 2024年第2期609-620,共12页
We analyze the significance of supersymmetry in two topological models and the standard model (SM). We conclude that the two topological field theory models favor hidden supersymmetry. The SM superpartners, instead, h... We analyze the significance of supersymmetry in two topological models and the standard model (SM). We conclude that the two topological field theory models favor hidden supersymmetry. The SM superpartners, instead, have not been found. 展开更多
关键词 Topological Field Theory SUPERSYMMETRY Chern-Simons Model
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英国医疗服务创新模式分析——以NHS西北创新中心TrusTECH为例 被引量:1
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作者 李靖华 Ronnie Ramlogan +3 位作者 Emma Malpeli 刘馨 Davide Consoli 王祺 《科技管理研究》 北大核心 2011年第2期14-19,共6页
英国医疗服务创新成果显著,值得借鉴。首先简要介绍了英国国家医疗服务体系(NHS)及其创新系统的主要情况,接着以NHS的西北创新中心TrusTECH为例,介绍了它的两种创新模式——商业化创新和服务创新计划,然后通过三个案例(生理学追踪和触... 英国医疗服务创新成果显著,值得借鉴。首先简要介绍了英国国家医疗服务体系(NHS)及其创新系统的主要情况,接着以NHS的西北创新中心TrusTECH为例,介绍了它的两种创新模式——商业化创新和服务创新计划,然后通过三个案例(生理学追踪和触发系统、信息管理工作手册和戒烟服务)深入分析了TrusTECH的服务创新流程和特点。最后总结了英国医疗服务创新模式的理论启示,以及对我国医疗服务创新的实践启示,包括培养医护人员创新意识和能力,建立系统化的创新平台,实现平台上的知识共享,以及重视非商业化创新等。 展开更多
关键词 创新模式 服务创新 医疗保健 英国 nhs
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健康信息服务驱动公共图书馆未来发展——基于《英国NHS图书馆质量评估标准》的分析与启示 被引量:33
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作者 王培林 《图书情报知识》 CSSCI 北大核心 2018年第2期32-39,共8页
《英国NHS图书馆质量评估标准》是英国官方的健康图书馆信息服务质量评估标准,规定了包括政策、经费、员工、服务设施、图书馆信息服务等在内的48个健康信息服务质量标准,将图书馆健康信息服务纳入到国家发展规划日程。以《英国NHS图书... 《英国NHS图书馆质量评估标准》是英国官方的健康图书馆信息服务质量评估标准,规定了包括政策、经费、员工、服务设施、图书馆信息服务等在内的48个健康信息服务质量标准,将图书馆健康信息服务纳入到国家发展规划日程。以《英国NHS图书馆质量评估标准》为切入点,深入解读其服务政策、员工资质、服务空间、资金来源、服务内容等五项涉及公众健康信息服务的重要质量标准,以期找到英国图书馆健康信息服务的成功所在。从紧密围绕国家政策指示、创设高体验值服务环境、争取社会力量支持、开展多元化服务推广、倡导健康知识服务等方面为我国公共图书馆健康信息服务的发展提供参考,以期推动我国图书馆健康信息服务的理论研究与实践创新。 展开更多
关键词 健康信息服务 公共图书馆 英国nhs图书馆质量评估标准 公众 服务推广
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Dual Instinct and Structural Theories: DSM-5-TR
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作者 Lawrence Greenman 《Open Journal of Psychiatry》 2024年第4期374-379,共6页
The genetic based Dual Instinct Theory is determined by evolutionary history. Aggression is required for survival and sex for propagation of species. Aggressive and sexual drive derivatives with their corresponding de... The genetic based Dual Instinct Theory is determined by evolutionary history. Aggression is required for survival and sex for propagation of species. Aggressive and sexual drive derivatives with their corresponding defence mechanisms are combined with biologically based stages of infant/child development and the functional entity of Structural Theory. This model of human nature is the applied to the diagnostic categories of DSM-5-TR. Objectives: To follow the innate Dual Instinct Theory from life to death and through Artificial Intelligence;connect it with biological stages of development of the Structural Theory and illustrate its manifestations in DSM-5-TR classifications. Method: Review of selected published literature. Applying the principle of focus and cognition of informed clinical observation of innate drive derivatives in conjunction with The Structural Theory. Both theories are functional entities with no structures involved. Sigmund Freud’s biologically based stages of development;oral, anal, genital pubic, adult, and geriatric along with a variety of unconscious, automatic, and persistent defense mechanisms are selectively folded into diagnosis listed in the manual. 展开更多
关键词 Dual Instincts Structural Theory Stages of Development Drive Derivatives Mechanisms of Defense
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NHS制度背景下英国医药产业政策体系探析与启示 被引量:6
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作者 李洁 David Qian 《中国卫生事业管理》 北大核心 2016年第11期806-808,843,共4页
目的:通过剖析NHS制度背景下英国医药产业政策体系的做法与特色,提出了完善我国产业政策体系的启示与建议。方法:采用文献研究法,对英国医药产业政策相关制度文件资料进行研究,剖析NHS制度下英国医药产业政策体系框架与内容。结论:借鉴... 目的:通过剖析NHS制度背景下英国医药产业政策体系的做法与特色,提出了完善我国产业政策体系的启示与建议。方法:采用文献研究法,对英国医药产业政策相关制度文件资料进行研究,剖析NHS制度下英国医药产业政策体系框架与内容。结论:借鉴英国经验,明确医药产业政策目标取向,形成有效协商机制,强化新药审批,引入药物经济学评价等,促进我国现有卫生制度下医药产业健康可持续发展。 展开更多
关键词 nhs 医药产业政策体系 英国 政策启示
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NHS-MAG_3为螯合剂的^(99m)Tc-寡核苷酸标记特性 被引量:1
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作者 张春 谭天秩 +3 位作者 匡安仁 李云春 郑建国 潘卫民 《四川大学学报(医学版)》 CAS CSCD 北大核心 2004年第2期169-171,共3页
目的 探讨以 NHS- MAG3作为螯合剂的 99m Tc-寡核苷酸 (99m Tc- ON)的标记特性。方法 将 NHS-MAG3与长度为 15个碱基的 c- m yc m RNA的反义 (ASON)、正义 (SON)和无义寡核苷酸 (MON)偶联 ,进行 99m Tc标记 ,Sephadex G2 5柱层析分离... 目的 探讨以 NHS- MAG3作为螯合剂的 99m Tc-寡核苷酸 (99m Tc- ON)的标记特性。方法 将 NHS-MAG3与长度为 15个碱基的 c- m yc m RNA的反义 (ASON)、正义 (SON)和无义寡核苷酸 (MON)偶联 ,进行 99m Tc标记 ,Sephadex G2 5柱层析分离纯化 ,评价 99m Tc- ON的标记率、放化纯和稳定性 ;将 ON- MAG3偶联物置 - 2 0℃贮存 15 d、1月、2月后进行 99m Tc标记 ,观察标记率的变化 ,评价 ON- MAG3的稳定性 ;用三氯乙酸沉淀法测定 99m Tc-ON的血浆蛋白结合率。结果  99m Tc- ASON、99m Tc- SON和 99m Tc- MON的标记率分别为 6 8.4 1%、6 6 .2 4 %和6 9.38% ,纯化后放化纯分别为 96 .98%、95 .34%和 94 .6 2 %。三者在室温和血清中均较稳定。 ON- MAG3在 - 2 0℃保存 2月后 ,标记率未见明显下降。三种 99m Tc- ON的血浆蛋白结合率均小于 13%。结论 以 NHS- MAG3作为螯合剂的 99m Tc- ON具备优良的标记特性 ,标记率、放化纯较高 ,稳定性好 ,血浆蛋白结合率低 。 展开更多
关键词 nhs-MAG3 C-MYC 寡核苷酸 反义 ^99MTC标记
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