Tyrosine phosphorylation is crucial for controlling normal cell growth,survival,intercellular communication,gene transcription,immune responses,and other processes.protein tyrosine phosphatase(PTP)and protein tyrosine...Tyrosine phosphorylation is crucial for controlling normal cell growth,survival,intercellular communication,gene transcription,immune responses,and other processes.protein tyrosine phosphatase(PTP)and protein tyrosine kinases(PTK)can achieve this goal by regulating multiple signaling pathways.Oedaleus decorus asiaticus is an important pest that infests the Mongolian Plateau grassland.We aimed to evaluate the survival rate,growth rate,overall performance,and ovarian developmental morphology of the 4th instar nymphs of O.decorus asiaticus while inhibiting the activity of protein tyrosine phosphatase-1B(PTP1B)and PTK.In addition,the expression and protein phosphorylation levels of key genes in the MAPK signaling pathway and antioxidant enzyme activity were assessed.The results showed no significant differences in survival rate,growth rate,or overall performance between PTP1B inhibitor treatment and control.However,after PTK inhibitor treatment,these indexes were significantly lower than those in the control.The ovarian size of female larvae after 15 days of treatment with PTK inhibitors showed significantly slower development,while female larvae treated with PTP1B exhibited faster ovarian growth than the control group.In comparison to controls and nymphs treated with PTK inhibitors,the expression and phosphorylation levels of key genes in the MAPK signaling pathway under PTP1B inhibitor treatments were significantly higher in 4th instar nymphs.However,reactiveoxygen(ROS)species levels and the activities of NADPH oxidase and other antioxidant enzymes were considerably reduced,although they were significantly greater in the PTK inhibitor treatment.The results suggest that PTP1B and PTK feedback inhibition in the mitogen-activated-protein kinases(MAPK)signal transfer can regulate the physiological metabolism of the insect as well as its developmental rate.These findings can facilitate future uses of PTP1B and PTK inhibitors in controlling insect development to help control pest populations.展开更多
随着4K、8K技术的快速发展,广播电视制作与播出领域进行了网际互连协议(Internet Protocol,IP)化的迭代,为高分辨率节目的传输提供更加快捷、灵活的应用环境。在这一过程中,同步时钟起到了重要的保障作用。基于此,分析IP系统中精确时间...随着4K、8K技术的快速发展,广播电视制作与播出领域进行了网际互连协议(Internet Protocol,IP)化的迭代,为高分辨率节目的传输提供更加快捷、灵活的应用环境。在这一过程中,同步时钟起到了重要的保障作用。基于此,分析IP系统中精确时间协议(Precision Time Protocol,PTP)时钟同步原理,阐述PTP在不同场景的应用,探讨如何构建一个统一基准的同步系统,以满足广电系统IP化的需求。展开更多
本文探讨了一种采用通用IT服务器平台实现专业媒体网络下IP流时钟同步精度分析和监测方法,实现对基于SMPTE ST 2110、SMPTE ST 2022-6等多种专业媒体网络IP流的时钟锁定精度状态进行实时监测和分析,同时该平台具备多通道IP流同时分析的...本文探讨了一种采用通用IT服务器平台实现专业媒体网络下IP流时钟同步精度分析和监测方法,实现对基于SMPTE ST 2110、SMPTE ST 2022-6等多种专业媒体网络IP流的时钟锁定精度状态进行实时监测和分析,同时该平台具备多通道IP流同时分析的能力,并提供信息上报接口。展开更多
基金the National Key R&D Program of China(2022YFD1400500)the earmarked fund for China Agriculture Research System(CARS-34)the Central Public-interest Scientific Institution Basal Research Fund of China(S2022XM21)。
文摘Tyrosine phosphorylation is crucial for controlling normal cell growth,survival,intercellular communication,gene transcription,immune responses,and other processes.protein tyrosine phosphatase(PTP)and protein tyrosine kinases(PTK)can achieve this goal by regulating multiple signaling pathways.Oedaleus decorus asiaticus is an important pest that infests the Mongolian Plateau grassland.We aimed to evaluate the survival rate,growth rate,overall performance,and ovarian developmental morphology of the 4th instar nymphs of O.decorus asiaticus while inhibiting the activity of protein tyrosine phosphatase-1B(PTP1B)and PTK.In addition,the expression and protein phosphorylation levels of key genes in the MAPK signaling pathway and antioxidant enzyme activity were assessed.The results showed no significant differences in survival rate,growth rate,or overall performance between PTP1B inhibitor treatment and control.However,after PTK inhibitor treatment,these indexes were significantly lower than those in the control.The ovarian size of female larvae after 15 days of treatment with PTK inhibitors showed significantly slower development,while female larvae treated with PTP1B exhibited faster ovarian growth than the control group.In comparison to controls and nymphs treated with PTK inhibitors,the expression and phosphorylation levels of key genes in the MAPK signaling pathway under PTP1B inhibitor treatments were significantly higher in 4th instar nymphs.However,reactiveoxygen(ROS)species levels and the activities of NADPH oxidase and other antioxidant enzymes were considerably reduced,although they were significantly greater in the PTK inhibitor treatment.The results suggest that PTP1B and PTK feedback inhibition in the mitogen-activated-protein kinases(MAPK)signal transfer can regulate the physiological metabolism of the insect as well as its developmental rate.These findings can facilitate future uses of PTP1B and PTK inhibitors in controlling insect development to help control pest populations.
文摘随着4K、8K技术的快速发展,广播电视制作与播出领域进行了网际互连协议(Internet Protocol,IP)化的迭代,为高分辨率节目的传输提供更加快捷、灵活的应用环境。在这一过程中,同步时钟起到了重要的保障作用。基于此,分析IP系统中精确时间协议(Precision Time Protocol,PTP)时钟同步原理,阐述PTP在不同场景的应用,探讨如何构建一个统一基准的同步系统,以满足广电系统IP化的需求。
