Discrimination of Transgenic Rice Based on Near Infrared Reflectance Spectroscopy and Partial Least Squares Regression Discriminant Analysis

Near infrared reflectance spectroscopy （NIRS）, a non-destructive measurement technique, was combined with partial least squares regression discrimiant analysis （PLS-DA） to discriminate the transgenic （TCTP and mi166） and wild type （Zhonghua 11） rice. Furthermore, rice lines transformed with protein gene （OsTCTP） and regulation gene （Osmi166） were also discriminated by the NIRS method. The performances of PLS-DA in spectral ranges of 4 000-8 000 cm-1 and 4 000-10 000 cm-1 were compared to obtain the optimal spectral range. As a result, the transgenic and wild type rice were distinguished from each other in the range of 4 000-10 000 cm-1, and the correct classification rate was 100.0% in the validation test. The transgenic rice TCTP and mi166 were also distinguished from each other in the range of 4 000-10 000 cm-1, and the correct classification rate was also 100.0%. In conclusion, NIRS combined with PLS-DA can be used for the discrimination of transgenic rice.

Estimating Wheat Grain Protein Content Using Multi-Temporal Remote Sensing Data Based on Partial Least Squares Regression

Estimating wheat grain protein content by remote sensing is important for assessing wheat quality at maturity and making grains harvest and purchase policies. However, spatial variability of soil condition, temperature, and precipitation will affect grain protein contents and these factors usually cannot be monitored accurately by remote sensing data from single image. In this research, the relationships between wheat protein content at maturity and wheat agronomic parameters at different growing stages were analyzed and multi-temporal images of Landsat TM were used to estimate grain protein content by partial least squares regression. Experiment data were acquired in the suburb of Beijing during a 2-yr experiment in the period from 2003 to 2004. Determination coefficient, average deviation of self-modeling, and deviation of cross- validation were employed to assess the estimation accuracy of wheat grain protein content. Their values were 0.88, 1.30%, 3.81% and 0.72, 5.22%, 12.36% for 2003 and 2004, respectively. The research laid an agronomic foundation for GPC （grain protein content） estimation by multi-temporal remote sensing. The results showed that it is feasible to estimate GPC of wheat from multi-temporal remote sensing data in large area.

Simultaneous Spectrophotometric Determination of Three Components Including Deoxyschizandrin by Partial Least Squares Regression

The computer auxiliary partial least squares is introduced to simultaneously determine the contents of Deoxyschizandin, Schisandrin, r-Schisandrin in the extracted solution of wuweizi. Regression analysis of the experimental results shows that the average recovery of each component is all in the range from 98.9% to 110.3% , which means the partial least squares regression spectrophotometry can circumvent the overlappirtg of absorption spectrums of mlulti-components, so that sctisfactory results can be obtained without any scrapple pre-separation.

Partial Least Squares Regression Model to Predict Water Quality in Urban Water Distribution Systems

The water distribution system of one residential district in Tianjin is taken as an example to analyze the changes of water quality.Partial least squares(PLS) regression model,in which the turbidity and Fe are regarded as control objectives,is used to establish the statistical model.The experimental results indicate that the PLS regression model has good predicted results of water quality compared with the monitored data.The percentages of absolute relative error(below 15%,20%,30%) are 44.4%,66.7%,100%(turbidity) and 33.3%,44.4%,77.8%(Fe) on the 4th sampling point;77.8%,88.9%,88.9%(turbidity) and 44.4%,55.6%,66.7%(Fe) on the 5th sampling point.

Comparison of dimension reduction-based logistic regression models for case-control genome-wide association study:principal components analysis vs.partial least squares

With recent advances in biotechnology, genome-wide association study （GWAS） has been widely used to identify genetic variants that underlie human complex diseases and traits. In case-control GWAS, typical statistical strategy is traditional logistical regression （LR） based on single-locus analysis. However, such a single-locus analysis leads to the well-known multiplicity problem, with a risk of inflating type I error and reducing power. Dimension reduction-based techniques, such as principal component-based logistic regression （PC-LR）, partial least squares-based logistic regression （PLS-LR）, have recently gained much attention in the analysis of high dimensional genomic data. However, the perfor- mance of these methods is still not clear, especially in GWAS. We conducted simulations and real data application to compare the type I error and power of PC-LR, PLS-LR and LR applicable to GWAS within a defined single nucleotide polymorphism （SNP） set region. We found that PC-LR and PLS can reasonably control type I error under null hypothesis. On contrast, LR, which is corrected by Bonferroni method, was more conserved in all simulation settings. In particular, we found that PC-LR and PLS-LR had comparable power and they both outperformed LR, especially when the causal SNP was in high linkage disequilibrium with genotyped ones and with a small effective size in simulation. Based on SNP set analysis, we applied all three methods to analyze non-small cell lung cancer GWAS data.

A partial least-squares regression approach to land use studies in the Suzhou-Wuxi-Changzhou region

In several LUCC studies, statistical methods are being used to analyze land use data. A problem using conventional statistical methods in land use analysis is that these methods assume the data to be statistically independent. But in fact, they have the tendency to be dependent, a phenomenon known as multicollinearity, especially in the cases of few observations. In this paper, a Partial Least-Squares （PLS） regression approach is developed to study relationships between land use and its influencing factors through a case study of the Suzhou-Wuxi-Changzhou region in China. Multicollinearity exists in the dataset and the number of variables is high compared to the number of observations. Four PLS factors are selected through a preliminary analysis. The correlation analyses between land use and influencing factors demonstrate the land use character of rural industrialization and urbanization in the Suzhou-Wuxi-Changzhou region, meanwhile illustrate that the first PLS factor has enough ability to best describe land use patterns quantitatively, and most of the statistical relations derived from it accord with the fact. By the decreasing capacity of the PLS factors, the reliability of model outcome decreases correspondingly.

Based on Partial Least-squares Regression to Build up and Analyze the Model of Rice Evapotranspiration

During the course of calculating the rice evapotranspiration using weather factors,we often find that some independent variables have multiple correlation.The phenomena can lead to the traditional multivariate regression model which based on least square method distortion.And the stability of the model will be lost.The model will be built based on partial least square regression in the paper,through applying the idea of main component analyze and typical correlation analyze,the writer picks up some component from original material.Thus,the writer builds up the model of rice evapotranspiration to solve the multiple correlation among the independent variables (some weather factors).At last,the writer analyses the model in some parts,and gains the satisfied result.

Quantum partial least squares regression algorithm for multiple correlation problem

Partial least squares(PLS) regression is an important linear regression method that efficiently addresses the multiple correlation problem by combining principal component analysis and multiple regression. In this paper, we present a quantum partial least squares(QPLS) regression algorithm. To solve the high time complexity of the PLS regression, we design a quantum eigenvector search method to speed up principal components and regression parameters construction. Meanwhile, we give a density matrix product method to avoid multiple access to quantum random access memory(QRAM)during building residual matrices. The time and space complexities of the QPLS regression are logarithmic in the independent variable dimension n, the dependent variable dimension w, and the number of variables m. This algorithm achieves exponential speed-ups over the PLS regression on n, m, and w. In addition, the QPLS regression inspires us to explore more potential quantum machine learning applications in future works.

A Novel Extension of Kernel Partial Least Squares Regression

Based on continuum power regression(CPR) method, a novel derivation of kernel partial least squares(named CPR-KPLS) regression is proposed for approximating arbitrary nonlinear functions.Kernel function is used to map the input variables(input space) into a Reproducing Kernel Hilbert Space(so called feature space),where a linear CPR-PLS is constructed based on the projection of explanatory variables to latent variables(components). The linear CPR-PLS in the high-dimensional feature space corresponds to a nonlinear CPR-KPLS in the original input space. This method offers a novel extension for kernel partial least squares regression(KPLS),and some numerical simulation results are presented to illustrate the feasibility of the proposed method.

Characterizing and estimating rice brown spot disease severity using stepwise regression,principal component regression and partial least-square regression

Detecting plant health conditions plays a key role in farm pest management and crop protection. In this study, measurement of hyperspectral leaf reflectance in rice crop (Oryzasativa L.) was conducted on groups of healthy and infected leaves by the fungus Bipolaris oryzae (Helminthosporium oryzae Breda. de Hann) through the wavelength range from 350 to 2 500 nm. The percentage of leaf surface lesions was estimated and defined as the disease severity. Statistical methods like multiple stepwise regression, principal component analysis and partial least-square regression were utilized to calculate and estimate the disease severity of rice brown spot at the leaf level. Our results revealed that multiple stepwise linear regressions could efficiently estimate disease severity with three wavebands in seven steps. The root mean square errors (RMSEs) for training (n=210) and testing (n=53) dataset were 6.5% and 5.8%, respectively. Principal component analysis showed that the first principal component could explain approximately 80% of the variance of the original hyperspectral reflectance. The regression model with the first two principal components predicted a disease severity with RMSEs of 16.3% and 13.9% for the training and testing dataset, respec-tively. Partial least-square regression with seven extracted factors could most effectively predict disease severity compared with other statistical methods with RMSEs of 4.1% and 2.0% for the training and testing dataset, respectively. Our research demon-strates that it is feasible to estimate the disease severity of rice brown spot using hyperspectral reflectance data at the leaf level.

Boosting the partial least square algorithm for regression modelling

Boosting algorithms are a class of general methods used to improve the general periormance of regression analysis. The main idea is to maintain a distribution over the train set. In order to use the given distribution directly, a modified PLS algorithm is proposed and used as the base learner to deal with the nonlinear multivariate regression problems. Experiments on gasoline octane number prediction demonstrate that boosting the modified PLS algorithm has better general performance over the PLS algorithm.

基于PLSR和LSSVM模型的土壤水分高光谱反演

为对地下采矿扰动区表层土壤水分进行反演,以大柳塔煤矿52501工作面为例,利用无人机搭载成像光谱仪获取高光谱影像,对获取的光谱数据进行对数、倒数对数、一阶和包络线去除变换,结合地面采集的128个土壤水分数据,基于偏最小二乘回归(PLSR)和最小二乘支持向量机(LSSVM)构建土壤水分预测模型并验证其预测精度。结果表明,基于一阶变换的PLSR模型和LSSVM模型预测精度相对较好,一阶变换的PLSR模型建模集R^(2)_(c)和预测集R^(2)_(p)分别为0.7021和0.6405,均方根误差RMSE_(c)和RMSE_(p)分别为1.6384%和1.1034%,相对分析误差RPD_(p)为1.7263;一阶变换的LSSVM模型建模集R^(2)_(c)和预测集R^(2)_(p)分别为0.8125和0.5979,均方根误差RMSE_(c)和RMSE_(p)分别为1.2755%和1.3459%,相对分析误差RPD_(P)为1.6323。最终基于PLSR和LSSVM模型完成了土壤水分的制图,实现了土壤水分的空间预测,为该研究区植被引导修复中土壤水分精准提升提供了空间数据支持。

优化光谱指数结合PLSR的多金属矿区土壤As含量高光谱反演

砷(As)是我国多金属矿区的主要污染物之一,对环境、农业和人类健康构成严重威胁。近地高光谱技术具有快速、动态、无损、光谱分辨率高等优势,对于多金属矿区土壤As污染监测与综合治理具有巨大应用潜力。然而,由于受污染区域、土壤背景以及高光谱质量、光谱输入量等因素影响,高光谱反演模型的适用性和精度差异较大。本研究针对湘南某多金属矿区,基于Pearson相关性分析并结合变量投影重要性(VIP)准则,提取18种变换光谱形式下的单变量特征波段及4种光谱指数算法下的优化光谱指数作为光谱输入量,建立偏最小二乘回归(PLSR)模型,实现了矿区土壤As含量反演。结果表明:倒数(RT)、对数(L)、平方根(Sqrt)、标准正态变量变换二阶导(SNV_SD)等变换后的光谱数据与As含量具有较高的相关性;优化光谱指数能从二维光谱空间揭示As的光谱响应,相较于单变量特征波段,以优化光谱指数为自变量构建的模型性能更优;比值指数(RI)模型的R_(c)^(2)、RMSE_(c)、R_(p)^(2)、RMSE_(p)、RPD分别为0.908、50.8 mg/kg、0.949、35.6 mg/kg、4.45,是研究区土壤As含量反演的最优模型。单变量特征波段结合优化光谱指数预测土壤As含量具有较好的可行性,可为多金属矿区土壤As污染高光谱快速监测提供科学依据。

The Consistency of LSE Estimators in Partial Linear Regression Models under Mixing Random Errors

In this paper,we consider the partial linear regression model y_(i)=x_(i)β^(*)+g(ti)+ε_(i),i=1,2,...,n,where(x_(i),ti)are known fixed design points,g(·)is an unknown function,andβ^(*)is an unknown parameter to be estimated,random errorsε_(i)are(α,β)-mix_(i)ng random variables.The p-th(p>1)mean consistency,strong consistency and complete consistency for least squares estimators ofβ^(*)and g(·)are investigated under some mild conditions.In addition,a numerical simulation is carried out to study the finite sample performance of the theoretical results.Finally,a real data analysis is provided to further verify the effect of the model.

Estimating canopy closure density and above-ground tree biomass using partial least square methods in Chinese boreal forests

Boreal forests play an important role in global environment systems. Understanding boreal forest ecosystem structure and function requires accurate monitoring and estimating of forest canopy and biomass. We used partial least square regression （PLSR） models to relate forest parameters, i.e. canopy closure density and above ground tree biomass, to Landsat ETM＋ data. The established models were optimized according to the variable importance for projection （VIP） criterion and the bootstrap method, and their performance was compared using several statistical indices. All variables selected by the VIP criterion passed the bootstrap test （p〈0.05）. The simplified models without insignificant variables （VIP 〈1） performed as well as the full model but with less computation time. The relative root mean square error （RMSE%） was 29% for canopy closure density, and 58% for above ground tree biomass. We conclude that PLSR can be an effective method for estimating canopy closure density and above ground biomass.

基于GEE和PLSR的2001-2020年长三角地区植被覆盖时空变化及驱动力分析

基于google earth engine(GEE)云平台,以MODIS EVI影像为数据源,结合Theil-Sen Median趋势分析、Mann-Kendall显著性分析、偏最小二乘回归(PLSR)分析等方法,对2001-2020年长三角地区植被覆盖的时空演变特征及其对气候和人类活动的响应特征进行分析。结果表明,1)2001-2020年,研究区植被覆盖度在时序上呈上升趋势(从0.433增至0.474),整体增长速率约为0.16%·a^(-1)。在空间上呈现“西高东低、南北高中部低”的分布特征,高值区均值0.486,低值区均值0.374。2)植被覆盖动态变化以改善(76.29%)和稳定(8.0%)为主,主要分布在西部和南部;退化区域(22.71%)主要集中在城市扩张地区。3)人类活动因素对植被覆盖时空变化的驱动力(投影重要性>1)大于气候因素(投影重要性<0.5)。降水为主要的气候驱动因素。城镇化快速发展导致土地利用变化较大,总体上对植被覆盖起到了显著的正向影响,但也导致景观破碎度增加。研究长三角植被覆盖变化及驱动因素,对区域生态环境监测、保护和建设具有重要现实意义。

Application of Principal Component Regression with Dummy Variable in Statistical Downscaling to Forecast Rainfall

Statistical downscaling (SD) analyzes relationship between local-scale response and global-scale predictors. The SD model can be used to forecast rainfall (local-scale) using global-scale precipitation from global circulation model output (GCM). The objectives of this research were to determine the time lag of GCM data and build SD model using PCR method with time lag of the GCM precipitation data. The observations of rainfall data in Indramayu were taken from 1979 to 2007 showing similar patterns with GCM data on 1st grid to 64th grid after time shift (time lag). The time lag was determined using the cross-correlation function. However, GCM data of 64 grids showed multicollinearity problem. This problem was solved by principal component regression (PCR), but the PCR model resulted heterogeneous errors. PCR model was modified to overcome the errors with adding dummy variables to the model. Dummy variables were determined based on partial least squares regression (PLSR). The PCR model with dummy variables improved the rainfall prediction. The SD model with lag-GCM predictors was also better than SD model without lag-GCM.

NIR Hyperspectral Imaging Measurement of Sugar Content in Peach Using PLS Regression

Near infrared （NIR） hyperspectral imaging measurement of sugar content in peach was introauced. NIR spectral images （650～1 000 nm, resolution： 2 nm） of peach samples were captured with developed hyperspectral imaging setup. Partial least square （PLS） regression prediction model was developed to estimate the sugar content in peach; step-wise backward method was utilized to determine optimal wavelength subsets. Experimental results show that the calibration model with optimal wavelength subsets has a correlation coefficient of prediction of 0.97 and a standard error of prediction of 0.19, the prediction accuracy is higher than the calibration model applied over the whole wavelength, which proves that variable selection plays an important role in improving the prediction accuracy of PLS regression model.

Short Term Electric Load Prediction by Incorporation of Kernel into Features Extraction Regression Technique

Accurate load prediction plays an important role in smart power management system, either for planning, facing the increasing of load demand, maintenance issues, or power distribution system. In order to achieve a reasonable prediction, authors have applied and compared two features extraction technique presented by kernel partial least square regression and kernel principal component regression, and both of them are carried out by polynomial and Gaussian kernels to map the original features’ to high dimension features’ space, and then draw new predictor variables known as scores and loadings, while kernel principal component regression draws the predictor features to construct new predictor variables without any consideration to response vector. In contrast, kernel partial least square regression does take the response vector into consideration. Models are simulated by three different cities’ electric load data, which used historical load data in addition to weekends and holidays as common predictor features for all models. On the other hand temperature has been used for only one data as a comparative study to measure its effect. Models’ results evaluated by three statistic measurements, show that Gaussian Kernel Partial Least Square Regression offers the more powerful features and significantly can improve the load prediction performance than other presented models.