The response of enzyme and non-enzymatic antioxidants of Mn hyperaccumuator, Polygonum hydropiper （P. hydropiper）, to Mn stress was studied using hydroponics culture experiments to explore the mechanism of Mn tolera...The response of enzyme and non-enzymatic antioxidants of Mn hyperaccumuator, Polygonum hydropiper （P. hydropiper）, to Mn stress was studied using hydroponics culture experiments to explore the mechanism of Mn tolerance in this species. Results showed that both chlorophyll and carotenoid contents significantly （p〈0.05） decreased with increasing Mn treatment levels （0, 0.5, 1, 2, 4, and 8 mg/L） in hydroponics. The concentrations of malondialdehyde （MDA） and hydrogen peroxide （H202） in the root and shoot of P hydropiper were accumulated under Mn stress. Meanwhile, the anti-oxidative functions of several important enzymes, including superoxide dismutase （SOD）, catalase （CAT）, ascorbate peroxidase （APX） and peroxidase （POD） in plants were stimulated by Mn spike in leaves and roots, especially at low Mn stress; while sulfhydryl group （--SH） and glutathion （GSH） were likely involved in Mn detoxification ofP. hydropiper under high Mn stress.展开更多
OBJECTIVE To investigate the antihyperlipidemic effect of methanol and aqueous extracts of the leaves of Polygonum minus in acute hyperlipidemic rat model.METHODS Acute hyperlipidemia was chemically induced in Sprague...OBJECTIVE To investigate the antihyperlipidemic effect of methanol and aqueous extracts of the leaves of Polygonum minus in acute hyperlipidemic rat model.METHODS Acute hyperlipidemia was chemically induced in Sprague Dawley rats by using poloxamer 407(500mg·kg-1 of body weight;intraperitoneal).Increase in total cholesterol was confirmed after six hours of induction.The normal and hyperlipidemic control groups were administered with 1mL carboxymethylcellulose(CMC),the two test groups received aqueous and methanol extract of leaves of P.minus respectively(1000mg·kg-1;orally;suspended in 1% CMC)whereas reference standard treated group received atorvastatin(60mg·kg-1;orally;suspended in 1% CMC)once daily for 3consecutive days.Blood samples were collected at 10 th and 24 th hour of the study for total cholesterol and triglycerides determination,while terminal blood samples were collected at58 th hour for full lipids profile analysis.RESULTS In the present study,both methanol and aqueous extracts lowered the serum total cholesterol and triglycerides significantly(P<0.001 and P<0.05,respectively)when compared with the hyperlipidemic control,similar to the standard drug atorvastatin.Varying effects were observed for both extracts on the other lipid parameter studied.Methanol extract showed significant reduction in LDL(P<0.05),VLDL(P<0.01)and atherogenic index(AI;P<0.001)and it showed a significant elevation in HDL levels(P<0.05).On the other hand,the aqueous extract showed significant reduction only in VLDL and AI(P<0.05)but no increase in HDL levels.CONCLUSION Present study confirmed the antihyperlipidemic effect of leaves of P.minus in acute hyperlipidemic rat model.The study also suggested that the methanol extract possess higher antihyperlipidemic effect than aqueous extract.Currently a study is ongoing to evaluate the antihyperlipidemic effect of the methanol extract in high-fat diet-induced chronic hyperlipidemic rat model.展开更多
[Objectives]This study was conducted to investigate the volatile components of Polygonum chinense L.[Methods]The volatile components of the methanol extract,ethyl acetate extract,chloroform extract and petroleum ether...[Objectives]This study was conducted to investigate the volatile components of Polygonum chinense L.[Methods]The volatile components of the methanol extract,ethyl acetate extract,chloroform extract and petroleum ether extract of P.chinense were analyzed and determined by gas chromatography.[Results]The volatile components of the methanol extract from P.chinense were the most,and phenolic acids and ketones accounted for a relatively high proportion,among which pyrogallic acid had the highest content.The ethyl acetate extract contained the second most volatile components,mostly acids and esters,of whichβ-sitosterol had the highest content;the chloroform and petroleum ether extracts had relatively few types of volatile components,most of which were alkanes,and the content ofγ-sitosterol shared by the two was the highest.The common substances of the four extracts were palmitic acid and phytol.[Conclusions]This study provides a theoretical basis for the quality evaluation of P.chinense and a scientific basis for its resource development.展开更多
基金Project(41161057)supported by the National Natural Science Foundation of ChinaProject(Guikezhuan 14122008-2)supported by Guangxi Provincial Science and Technology Development,China+2 种基金Project(2014GXNSFAA118303)supported by the Natural Science Foundation of Guangxi Province,ChinaProjects(YRHJ15K002,YRHJ15Z026)supported by Key Laboratory of Karst Ecology and Environment Change of Guangxi Normal University,ChinaProject(2016JJ6135)supported by the Natural Science Foundation of Hunan Province,China
文摘The response of enzyme and non-enzymatic antioxidants of Mn hyperaccumuator, Polygonum hydropiper （P. hydropiper）, to Mn stress was studied using hydroponics culture experiments to explore the mechanism of Mn tolerance in this species. Results showed that both chlorophyll and carotenoid contents significantly （p〈0.05） decreased with increasing Mn treatment levels （0, 0.5, 1, 2, 4, and 8 mg/L） in hydroponics. The concentrations of malondialdehyde （MDA） and hydrogen peroxide （H202） in the root and shoot of P hydropiper were accumulated under Mn stress. Meanwhile, the anti-oxidative functions of several important enzymes, including superoxide dismutase （SOD）, catalase （CAT）, ascorbate peroxidase （APX） and peroxidase （POD） in plants were stimulated by Mn spike in leaves and roots, especially at low Mn stress; while sulfhydryl group （--SH） and glutathion （GSH） were likely involved in Mn detoxification ofP. hydropiper under high Mn stress.
基金The project supported in part by AIMST University Internal Grant(AURGC/18/FOP/2013)
文摘OBJECTIVE To investigate the antihyperlipidemic effect of methanol and aqueous extracts of the leaves of Polygonum minus in acute hyperlipidemic rat model.METHODS Acute hyperlipidemia was chemically induced in Sprague Dawley rats by using poloxamer 407(500mg·kg-1 of body weight;intraperitoneal).Increase in total cholesterol was confirmed after six hours of induction.The normal and hyperlipidemic control groups were administered with 1mL carboxymethylcellulose(CMC),the two test groups received aqueous and methanol extract of leaves of P.minus respectively(1000mg·kg-1;orally;suspended in 1% CMC)whereas reference standard treated group received atorvastatin(60mg·kg-1;orally;suspended in 1% CMC)once daily for 3consecutive days.Blood samples were collected at 10 th and 24 th hour of the study for total cholesterol and triglycerides determination,while terminal blood samples were collected at58 th hour for full lipids profile analysis.RESULTS In the present study,both methanol and aqueous extracts lowered the serum total cholesterol and triglycerides significantly(P<0.001 and P<0.05,respectively)when compared with the hyperlipidemic control,similar to the standard drug atorvastatin.Varying effects were observed for both extracts on the other lipid parameter studied.Methanol extract showed significant reduction in LDL(P<0.05),VLDL(P<0.01)and atherogenic index(AI;P<0.001)and it showed a significant elevation in HDL levels(P<0.05).On the other hand,the aqueous extract showed significant reduction only in VLDL and AI(P<0.05)but no increase in HDL levels.CONCLUSION Present study confirmed the antihyperlipidemic effect of leaves of P.minus in acute hyperlipidemic rat model.The study also suggested that the methanol extract possess higher antihyperlipidemic effect than aqueous extract.Currently a study is ongoing to evaluate the antihyperlipidemic effect of the methanol extract in high-fat diet-induced chronic hyperlipidemic rat model.
基金Supported by Development of Huotanmu Capsules,a New Drug for Hepatitis B Treatment(20183046-1)Collaborative Innovation Center for Zhuang and Yao Medicines(GJKY[2013]20)+2 种基金Guangxi Key Laboratory of Zhuang and Yao Medicines(GKJZ[2014]32)Guangxi Key Discipline:Zhuang Medicine(GJKY[2013]16)First-class Discipline in Guangxi of Traditional Chinese Pharmacology(GJKY[2018]12)。
文摘[Objectives]This study was conducted to investigate the volatile components of Polygonum chinense L.[Methods]The volatile components of the methanol extract,ethyl acetate extract,chloroform extract and petroleum ether extract of P.chinense were analyzed and determined by gas chromatography.[Results]The volatile components of the methanol extract from P.chinense were the most,and phenolic acids and ketones accounted for a relatively high proportion,among which pyrogallic acid had the highest content.The ethyl acetate extract contained the second most volatile components,mostly acids and esters,of whichβ-sitosterol had the highest content;the chloroform and petroleum ether extracts had relatively few types of volatile components,most of which were alkanes,and the content ofγ-sitosterol shared by the two was the highest.The common substances of the four extracts were palmitic acid and phytol.[Conclusions]This study provides a theoretical basis for the quality evaluation of P.chinense and a scientific basis for its resource development.
