[目的]分析DNA、引物,dNTPs、Taq DNA聚合酶4种因素对雷公笋SCoT-PCR扩增结果的影响,通过最优的SCoT-PCR反应体系筛选多态性引物,为雷公笋种质资源分子标记研究提供条件。[方法]以海南雷公笋为材料,采用单因素试验和正交试验方法。[结果...[目的]分析DNA、引物,dNTPs、Taq DNA聚合酶4种因素对雷公笋SCoT-PCR扩增结果的影响,通过最优的SCoT-PCR反应体系筛选多态性引物,为雷公笋种质资源分子标记研究提供条件。[方法]以海南雷公笋为材料,采用单因素试验和正交试验方法。[结果]Taq酶对雷公笋SCoT-PCR扩增的影响最大,其次是模板DNA和dNTPs,最后是引物;最优反应体系为20μL体系中40 ng DNA,0.30μmol/L引物,0.25 mmol/L dNTPs,3.00 U Taq酶。经验证,该体系获得的扩增产物清晰稳定;应用该体系从40条SCoT引物筛选出8条多态性好,且适合雷公笋扩增的引物,多态性条带占62.64%。[结论]该研究为使用SCoT分子标记技术对雷公笋开展深入研究提供了重要的理论基础和技术支持。展开更多
Over the past decade,the presence of mistletoe(Viscum album ssp.austriacum)in Scots pine stands has increased in many European countries.Understanding the factors that influence the occurrence of mistletoe in stands i...Over the past decade,the presence of mistletoe(Viscum album ssp.austriacum)in Scots pine stands has increased in many European countries.Understanding the factors that influence the occurrence of mistletoe in stands is key to making appropriate forest management decisions to limit damage and prevent the spread of mistletoe in the future.Therefore,the main objective of this study was to determine the probability of mistletoe occurrence in Scots pine stands in relation to stand-related endogenous factors such as age,top height,and stand density,as well as topographic and edaphic factors.We used unmanned aerial vehicle(UAV)imagery from 2,247 stands to detect mistletoe in Scots pine stands,while majority stand and site characteristics were calculated from airborne laser scanning(ALS)data.Information on stand age and site type from the State Forest database were also used.We found that mistletoe infestation in Scots pine stands is influenced by stand and site characteristics.We documented that the densest,tallest,and oldest stands were more susceptible to mistletoe infestation.Site type and specific microsite conditions associated with topography were also important factors driving mistletoe occurrence.In addition,climatic water balance was a significant factor in increasing the probability of mistletoe occurrence,which is important in the context of predicted temperature increases associated with climate change.Our results are important for better understanding patterns of mistletoe infestation and ecosystem functioning under climate change.In an era of climate change and technological development,the use of remote sensing methods to determine the risk of mistletoe infestation can be a very useful tool for managing forest ecosystems to maintain forest sustainability and prevent forest disturbance.展开更多
Ecoregion-based height-diameter models were developed in the present study for Scots pine(Pinus sylves-tris L.)stands in Turkiye and included several ecological factors derived from a pre-existing ecoregional classifi...Ecoregion-based height-diameter models were developed in the present study for Scots pine(Pinus sylves-tris L.)stands in Turkiye and included several ecological factors derived from a pre-existing ecoregional classification system.The data were obtained from 2831 sample trees in 292 sample plots.Ten generalized height–diameter models were developed,and the best model(HD10)was selected according to statistical criteria.Then,nonlinear mixed-effects modeling was applied to the best model.The R2 for the generalized height‒diameter model(Richards function)modified by Sharma and Parton is 0.951,and the final model included number of trees,dominant height,and diameter at breast height,with a random parameter associated with each ecoregion attached to the inverse of the mean basal area.The full model predictions using the nonlinear mixed-effects model and the reduced model(HD10)predictions were compared using the nonlinear sum of extra squares test,which revealed significant differences between ecore-gions;ecoregion-based height–diameter models were thus found to be suitable to use.In addition,using these models in appropriate ecoregions was very important for achieving reliable predictions with low prediction errors.展开更多
文摘[目的]分析DNA、引物,dNTPs、Taq DNA聚合酶4种因素对雷公笋SCoT-PCR扩增结果的影响,通过最优的SCoT-PCR反应体系筛选多态性引物,为雷公笋种质资源分子标记研究提供条件。[方法]以海南雷公笋为材料,采用单因素试验和正交试验方法。[结果]Taq酶对雷公笋SCoT-PCR扩增的影响最大,其次是模板DNA和dNTPs,最后是引物;最优反应体系为20μL体系中40 ng DNA,0.30μmol/L引物,0.25 mmol/L dNTPs,3.00 U Taq酶。经验证,该体系获得的扩增产物清晰稳定;应用该体系从40条SCoT引物筛选出8条多态性好,且适合雷公笋扩增的引物,多态性条带占62.64%。[结论]该研究为使用SCoT分子标记技术对雷公笋开展深入研究提供了重要的理论基础和技术支持。
基金funded by National Science Centre,Poland under the project"Assessment of the impact of weather conditions on forest health status and forest disturbances at regional and national scale based on the integration of ground and space-based remote sensing datasets"(project no.2021/41/B/ST10/)Data collection and research was also supported by the project no.EZ.271.3.19.2021"Modele ryzyka zamierania drzewostanow glownych gatunkow lasotworczych Polski"funded by the General Directorate of State Forests in Poland。
文摘Over the past decade,the presence of mistletoe(Viscum album ssp.austriacum)in Scots pine stands has increased in many European countries.Understanding the factors that influence the occurrence of mistletoe in stands is key to making appropriate forest management decisions to limit damage and prevent the spread of mistletoe in the future.Therefore,the main objective of this study was to determine the probability of mistletoe occurrence in Scots pine stands in relation to stand-related endogenous factors such as age,top height,and stand density,as well as topographic and edaphic factors.We used unmanned aerial vehicle(UAV)imagery from 2,247 stands to detect mistletoe in Scots pine stands,while majority stand and site characteristics were calculated from airborne laser scanning(ALS)data.Information on stand age and site type from the State Forest database were also used.We found that mistletoe infestation in Scots pine stands is influenced by stand and site characteristics.We documented that the densest,tallest,and oldest stands were more susceptible to mistletoe infestation.Site type and specific microsite conditions associated with topography were also important factors driving mistletoe occurrence.In addition,climatic water balance was a significant factor in increasing the probability of mistletoe occurrence,which is important in the context of predicted temperature increases associated with climate change.Our results are important for better understanding patterns of mistletoe infestation and ecosystem functioning under climate change.In an era of climate change and technological development,the use of remote sensing methods to determine the risk of mistletoe infestation can be a very useful tool for managing forest ecosystems to maintain forest sustainability and prevent forest disturbance.
基金supported by Scientific Research Projects Management Coordinator of Kastamonu University,under grant number KÜ-BAP01/2019-41.
文摘Ecoregion-based height-diameter models were developed in the present study for Scots pine(Pinus sylves-tris L.)stands in Turkiye and included several ecological factors derived from a pre-existing ecoregional classification system.The data were obtained from 2831 sample trees in 292 sample plots.Ten generalized height–diameter models were developed,and the best model(HD10)was selected according to statistical criteria.Then,nonlinear mixed-effects modeling was applied to the best model.The R2 for the generalized height‒diameter model(Richards function)modified by Sharma and Parton is 0.951,and the final model included number of trees,dominant height,and diameter at breast height,with a random parameter associated with each ecoregion attached to the inverse of the mean basal area.The full model predictions using the nonlinear mixed-effects model and the reduced model(HD10)predictions were compared using the nonlinear sum of extra squares test,which revealed significant differences between ecore-gions;ecoregion-based height–diameter models were thus found to be suitable to use.In addition,using these models in appropriate ecoregions was very important for achieving reliable predictions with low prediction errors.
