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Magnetic resonance imaging of hepatocellular carcinoma induced by diethylnitrosamine in Sprague-Dawley rats 被引量:3
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作者 Department of Radiology, West China Hospital, Sichuan University, Chengdu 610041, China (Li X, Zhou XP and Guan YS) Department of Radiology, Ruijin Hospieal. Shanghai Second Medical University, Shanghai 200025 , China. Current address:Astra Zeneca R & D Alderley, Macclesfield SK 10 4 TG U. K. (Wang YX) 《Hepatobiliary & Pancreatic Diseases International》 SCIE CAS 2005年第3期427-432,共6页
Diethylnitrosamine (DENA) is able to induce various benign and malignant liver lesions in rats with a high success rate and a low mortality rate. It provides a more appropriate model that better simulates the various ... Diethylnitrosamine (DENA) is able to induce various benign and malignant liver lesions in rats with a high success rate and a low mortality rate. It provides a more appropriate model that better simulates the various lesions occurring in humans than the usual model of tumor implantations. The aim of the present study was to evaluate MRI liver examination in Sprague-Dawley(SD) rats as a routine method to detect hepatocellular carcinoma (HCC) nodules induced by DENA and to follow up their growth. METHODS:Hepatic carcinogenesis was induced in 80 male SD rats using oral DENA solution. All animals were imaged for liver tumor detection with a 1.5 Tesla magnet (Siemens Sonata,Erlangen, Germany) using correspondence scan parameters and a radio-frequency knee coil. Macroscopic examinations were performed along the axial MRI sections to evaluate magnetic resonance imaging (MRI) findings, and histopathological assessment was also performed. RESULTS:No false negative results were obtained on MR images. Hepatic tumors in 72 rats were confirmed macro-scopically and 68 rats were detected by MRI till the 20th week. The smallest and the largest nodules detected by MRI were 2 mm and 37 mm in diameter respectively. The agreement rate of MRI with macroscopic observation was 39. 1% and 97. 4% respectively for 2 mm to 5 mm and more than 5 mm nodules. CONCLUSIONS:The hepatic tumor induced by DENA provides a more representative range of tumors for imaging diagnosis and interventional treatment. MRI is the best approach for scrutinizing pathological changes of rat livers in the period of observation. 展开更多
关键词 ANIMAL-MODEL hepatocellular carcinoma sprague-dawley rat magnetic resonance imaging
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Analysis of transcriptome sequencing of sciatic nerves in Sprague-Dawley rats of different ages 被引量:1
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作者 Jiang-Hui Liu Qing Tang +5 位作者 Xiang-Xia Liu Jian Qi Rui-Xi Zeng Zhao-Wei Zhu Bo He Yang-Bin Xu 《Neural Regeneration Research》 SCIE CAS CSCD 2018年第12期2182-2190,共9页
An aging-induced decrease in Schwann cell viability can affect regeneration following peripheral nerve injury in mammals. It is therefore necessary to investigate possible age-related changes in gene expression that m... An aging-induced decrease in Schwann cell viability can affect regeneration following peripheral nerve injury in mammals. It is therefore necessary to investigate possible age-related changes in gene expression that may affect the biological function of peripheral nerves. Ten 1-week-old and ten 12-month-old healthy male Sprague-Dawley rats were divided into young(1 week old) and adult(12 months old) groups according to their ages. mRNA expression in the sciatic nerve was compared between young and adult rats using next-generation sequencing(NGS) and bioinformatics(n = 4/group). The 18 groups of differentially expressed mRNA(DEmRNAs) were also tested by quantitative reverse transcription polymerase chain reaction(n = 6/group). Results revealed that(1) compared with young rats, adult rats had 3608 groups of DEmRNAs. Of these, 2684 were groups of upregulated genes, and 924 were groups of downregulated genes. Their functions mainly involved cell viability, proliferation, differentiation, regeneration, and myelination.(2) The gene with the most obvious increase of all DEmRNAs in adult rats was Thrsp(log2 FC = 9.01, P 〈 0.05), and the gene with the most obvious reduction was Col2 a1(log2 FC = -8.89, P 〈 0.05).(3) Gene Ontology analysis showed that DEmRNAs were mainly concentrated in oligosaccharide binding, nucleotide-binding oligomerization domain containing one signaling pathway, and peptide-transporting ATPase activity.(4) Analysis using the Kyoto Encyclopedia of Genes and Genomes showed that, with increased age, DEmRNAs were mainly enriched in steroid biosynthesis, Staphylococcus aureus infection, and graft-versus-host disease.(5) Spearman's correlation coefficient method for evaluating NGS accuracy showed that the NGS results and quantitative reverse transcription polymerase chain reaction results were positively correlated(rs = 0.74, P 〈 0.05). These findings confirm a difference in sciatic nerve gene expression between adult and young rats, suggesting that, in peripheral nerves, cells and the microenvironment change with age, thus influencing the function and repair of peripheral nerves. 展开更多
关键词 peripheral nerve injury AGING sprague-dawley rat TRANSCRIPTOME SEQUENCING MRNA rat age Schwann cells neural regeneration
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Hormetic Effects of Yttrium on Male Sprague-Dawley Rats 被引量:2
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作者 ZHANG Wen Zhong SUN Na Na +3 位作者 MA Shang Quan ZHAO Zhen Chao CAO Yu ZHANG Chao 《Biomedical and Environmental Sciences》 SCIE CAS CSCD 2018年第10期777-780,共4页
To evaluate hormesis induced by Yttrium(Y) nitrate in male rats, Y was offered to F0 mother rats and F1 offspring at concentrations of 0, 20, 80, and320 ppm daily from gestational day (GD) 0 through postnatal day... To evaluate hormesis induced by Yttrium(Y) nitrate in male rats, Y was offered to F0 mother rats and F1 offspring at concentrations of 0, 20, 80, and320 ppm daily from gestational day (GD) 0 through postnatal day 70(PND70).The F1 offspring were evaluated with respect to motor function,learning and memory, and histopathology. 展开更多
关键词 PND Hormetic Effects of Yttrium on Male sprague-dawley rats
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Differences in action potential propagation speed and axon initial segment plasticity between neurons from Sprague-Dawley rats and C57BL/6 mice
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作者 Zhi-Ya Chen Luxin Peng +5 位作者 Mengdi Zhao Yu Li Mochizuki Takahiko Louis Tao Peng Zou Yan Zhang 《Zoological Research》 SCIE CAS CSCD 2022年第4期615-633,共19页
Action potentials(APs)in neurons are generated at the axon initial segment(AIS).AP dynamics,including initiation and propagation,are intimately associated with neuronal excitability and neurotransmitter release kineti... Action potentials(APs)in neurons are generated at the axon initial segment(AIS).AP dynamics,including initiation and propagation,are intimately associated with neuronal excitability and neurotransmitter release kinetics.Most learning and memory studies at the single-neuron level have relied on the use of animal models,most notably rodents.Here,we studied AP initiation and propagation in cultured hippocampal neurons from Sprague-Dawley(SD)rats and C57BL/6(C57)mice with genetically encoded voltage indicator(GEVI)-based voltage imaging.Our data showed that APs traveled bidirectionally in neurons from both species;forward-propagating APs(fpAPs)had a different speed than backpropagating APs(bpAPs).Additionally,we observed distinct AP propagation characteristics in AISs emerging from the somatic envelope compared to those originating from dendrites.Compared with rat neurons,mouse neurons exhibited higher bpAP speed and lower fpAP speed,more distally located ankyrin G(AnkG)in AISs,and longer Nav1.2 lengths in AISs.Moreover,during AIS plasticity,AnkG and Nav1.2 showed distal shifts in location and shorter lengths of labeled AISs in rat neurons;in mouse neurons,however,they showed a longer AnkG-labeled length and more distal Nav1.2 location.Our findings suggest that hippocampal neurons in SD rats and C57 mice may have different AP propagation speeds,different AnkG and Nav1.2 patterns in the AIS,and different AIS plasticity properties,indicating that comparisons between these species must be carefully considered. 展开更多
关键词 sprague-dawley rats C57BL/6 mice Action potential Axon initial segment PLASTICITY
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Effects of chromium nanoparticle dosage on growth,body composition,serum hormones and tissue chromium in Sprague-Dawley rats 被引量:4
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作者 ZHA Long-ying XU Zi-rong WANG Min-qi GU Liang-ying 《Journal of Zhejiang University-Science B(Biomedicine & Biotechnology)》 SCIE CAS CSCD 2007年第5期323-330,共8页
This 6-week study was conducted to evaluate the effects of seven different levels of dietary chromium (Cr) (0,75,150,300,450,600,and 1200 ppb Cr) in the form of Cr nanoparticle (CrNano) on growth,body composition,seru... This 6-week study was conducted to evaluate the effects of seven different levels of dietary chromium (Cr) (0,75,150,300,450,600,and 1200 ppb Cr) in the form of Cr nanoparticle (CrNano) on growth,body composition,serum hormones and tissue Cr in Sprague-Dawley (SD) rats. Seventy male SD rats (average initial body weight of (83.2±4.4) g) were randomly assigned to seven dietary treatments (n=10). At the end of the trial,body composition was assessed via dual energy X-ray absorptiometry (DEXA). All rats were then sacrificed to collect samples of blood,organs and tissues for determination of serum hormones and tissue Cr contents. The results indicated that lean body mass was significantly increased (P<0.05) due to the addition of 300 and 450 ppb Cr from CrNano. Supplementation of 150,300,450,and 600 ppb Cr decreased (P<0.05) percent body fat significantly. Average daily gain was increased (P<0.05) by addition of 75,150,and 300 ppb Cr and feed efficiency was increased (P<0.05) by supplementation of 75,300,and 450 ppb Cr. Addition of 300 and 450 ppb Cr decreased (P<0.05) the insulin level in serum greatly. Cr contents in liver and kidney were greatly increased (P<0.05) by the addition of Cr as CrNano in the dosage of from 150 ppb to 1 200 ppb. In addition,Supplementation of 300,450,and 600 ppb Cr significantly increased (P<0.05) Cr content in the hind leg muscle. These results suggest that supplemental CrNano has beneficial effects on growth performance and body composition,and increases tissue Cr concentration in selected muscles. 展开更多
关键词 rat Chromium (Cr) NANOPARTICLE GROWTH Body composition HORMONE
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Effects of Estrogen on ER, NGF, and ChAT Expression in Cerebellum of Aging Female Sprague-Dawley Rat 被引量:1
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作者 CHEN Zheng-li FAN Guang-li +2 位作者 LUO Qi-hui ZHU Chun-mei HUANG Yi-dan 《Agricultural Sciences in China》 CAS CSCD 2007年第3期368-374,共7页
This article discusses the effects of estrogen on the expression of estrogen receptor (ER), nerve growth factor (NGF), and choline acetyltransferase (CHAT) in the cerebellum of rats. The model of aging female ra... This article discusses the effects of estrogen on the expression of estrogen receptor (ER), nerve growth factor (NGF), and choline acetyltransferase (CHAT) in the cerebellum of rats. The model of aging female rat was established to study the expression and distribution of ER, NGF, and ChAT in the cerebellum following 17β-estradiol treatment using the technique of immunohistochemical ultrasensitive SP in sprague-dawley rat. The immunoreactive productions were distributed in stratum Purkinje cell, nucleus dentatus, nucleus interpositus, and nucleus fastigii of cerebellum, and the ER positive production was mainly located in the plasma, cytoplasmic membrane, and neurite, and also existed in nucleus. The general tendency of the expression of ER, NGF, and ChAT positive production in the cerebellum cortex and nuclei of aging rat significantly decreases, while the intensity and quantity of the immunoreactive production ascends predominantly after 17β-estradiol treatment. Simultaneously, the positive neurite of Purkinje cell shows a similar tendency. The above- mentioned results suggest that the estrogen upregulates the expression of NGF and CHAT, and plays a vital role in sustaining and protecting the structure and function of cerebellum neurons. Furthermore, the similarity of their changing tendency implies that they were correlated and cooperated during the course in effect of estrogen on cerebellum. It also showed that the action of estrogen in cerebellum could be via genomic and nongenomic mechanism. 展开更多
关键词 estrogen receptor (ER) nerve growth factor (NGF) choline acetyltransferase (CHAT) immunohistochemical ultrasensitive SP CEREBELLUM rat
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Analysis of Behaviour Pattern in Sprague-Dawley Rats Using Microwave Techniques
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作者 Anil Lonappan Ernest Remy Hervé Ebell’a Dallé +1 位作者 Thomas Joachim Odhiambo Afullo Williams Mark Uren Daniels 《Journal of Electromagnetic Analysis and Applications》 2017年第11期155-166,共12页
This communication presents a new method of detecting the behaviour pattern in Sprague-Dawley (SD) rats based on the measurement of the dielectric properties of blood plasma at microwave frequencies at different perio... This communication presents a new method of detecting the behaviour pattern in Sprague-Dawley (SD) rats based on the measurement of the dielectric properties of blood plasma at microwave frequencies at different periods of time. The microwave measurements were performed by rectangular cavity perturbation method in the S-band of microwave frequency with the blood plasma collected from normal rats (Controls) as well as chemically induced rats (A&beta;). A change is observed in the dielectric properties of the A&beta;samples but not the controls samples at the extended period of time. This measurement technique is simple and the collection of blood from the rats is nonsurgical in nature. These results prove a new method of diagnosing Alzheimer’s Disease (AD) using microwave techniques. 展开更多
关键词 BLOOD Plasma sprague-dawley rats CAVITY PERTURBATION Alzheimer’s DISEASE
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Establishment of Allergic Airway Inflammation Model in Late-phase Response of Sprague-Dawley Rats
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作者 朱敏敏 傅诚章 周钦海 《Journal of Nanjing Medical University》 2002年第4期163-167,共5页
Objective To establish allergic airway inflammation model in late-phase airway reaction of Sprague-Dawley (SD) rats. Methods Thirty-six SD rats were randomly divided into three groups-, control group (Group Ⅰ) ,singl... Objective To establish allergic airway inflammation model in late-phase airway reaction of Sprague-Dawley (SD) rats. Methods Thirty-six SD rats were randomly divided into three groups-, control group (Group Ⅰ) ,single challenge group (Group Ⅱ ),consecutive challenge group (Group Ⅲ). The rats in Group Ⅱ and Group Ⅲ were sensitized twice by injection of ovalbumin (OA) together with aluminum hydroxide and Bordetella pertussis as adjuvants, followed by challenge with aerosolized OA for 20 min once in Group Ⅱ or one time on each day for one week in, Group Ⅲ. The rats in Group Ⅰ received 0. 9% saline by injection and inhalation. Results Compared with group Ⅰ , there were positive symptoms observed in the group Ⅱ and group Ⅲ; the amount of total leucocytes and eosinophil percentage in brochoalveolar lavage fluid (BALF) significantly increased (P<0.05 or P<0. 01 respectively) in Group Ⅱ or Ⅲ ; histopathologic changes of lung showed acute allergic inflammation changes in Group Ⅱ: Disrupted epithelium damaged subepithelial structure and eosinophil infiltration in the airway wall. As for the Group Ⅲ, there were allergen- induced characteristic features of chronic allergic airways inflammation: hypertrophy and hyperplasia of bronchial smooth muscle, goblet cell hyperplasia, basement membrane thickening, eosinophil in filtration, edema. Conclusion The model of allergic airway inflammation in late-phase response of SD rats was successfully established by OA sensitization (twice) and consecutive challenge. 展开更多
关键词 allergic airway inflammation late-phase airway reaction rat MODEL
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Comparative study on the difference of obesity model induced by two kinds of high fat diet in Sprague-Dawley rats
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作者 Shanlei Zhang Wenqing Mo +3 位作者 Xi Rong Fang Wei Hong Liu Fuling Huang 《广西医科大学学报》 CAS 2018年第1期1-5,共5页
Objective: To explore the differences of obese Sprague-Dawley(SD)rats model induced by lard oil high-fat(HF)diet or purified HF diet. Methods: SD weanling rats were randomly divided into three groups: D1 group,where r... Objective: To explore the differences of obese Sprague-Dawley(SD)rats model induced by lard oil high-fat(HF)diet or purified HF diet. Methods: SD weanling rats were randomly divided into three groups: D1 group,where rats were fed by lard oil HF diet;D2 group,where rats were fed by purified HF diet;C group,where rats were fed on chow. After 12 weeks,diet-induced obesity rat(stop 33% based on weight)were selected for further study,and the rest rats from group D1 and D2 were excluded. The food intake and weight were weighted daily and weekly,respectively. The subcutaneous,visceral and total fat contents of rats was measured by 256-row CT scan and the Lee index was calculated accordingly. The kidney,liver,testis,spleen and heart were weighted respectively. Serum leptin and insulin levels were quantified. The pathology in liver and adipose tissues were analyzed by HE staining. Oral glucose tolerance test(OGTT)was used to compare the glucose tolerance ability. Serum total cholestero(lT-CHO),high density lipoprotein(HDL-C),low density lipoprotein(LDL-C),triglyceride(TG)and inflammatory cytokines IL-6,TNF-α were detected as well. Results: After 12 weeks,the body weight,subcutaneous fat,visceral fat,total fat mass,wet weight of liver,kidney and heart,area under blood glucose curve and the levels of serum insulin,leptin,T-CHO,LDL-C,TG,IL-6 and TNF-α in group D2 were significantly increased compared to those of group C and group D1. HDL-C of group D2 was markedly lower than that in group C(P<0. 05). The visceral fat,total fat content and HDL-C in group D1 were significantly different from those of group C(P<0. 05). Steatosis and enlarged adipocyte were found in the livers of rats in group D1 and D2,and the lesions were more significant in group D2. Conclusion: Purified HF diet was more effective in inducing metabolic abnormalities,steatosis,peripheral chronic inflammation in obese SD rat models. But lard oil HF diet was more economical when only inducing visceral steatosis was required. 展开更多
关键词 high fat diet weanling SD rats diet-induced obesity model
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Retinal damage after exposure to white light emitting diode lights at different intensities in Sprague-Dawley rats
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作者 Guangsen Wu Xiaoyou Huang +5 位作者 He Meng Le Yang Shengjian Lin Yang Gao Yujun Li Yandong Wang 《Annals of Eye Science》 2019年第1期89-96,共8页
Background:The usage of the light emitting diode(LED)has been increasingly applied in the illumination setting and electronic equipment.However,the effect of LED lights on the retina remains unclear.In this study,we o... Background:The usage of the light emitting diode(LED)has been increasingly applied in the illumination setting and electronic equipment.However,the effect of LED lights on the retina remains unclear.In this study,we observed and analyzed the impact of white LED lights at different intensities on the function and morphology of rat retinas.Methods:Thirty-six Sprague-Dawley rats weighing 150-180 g were randomly divided into six groups(n=6 in each group)including a normal control(NC)group,4 white LED groups at different light intensities(4,000,6,000,7,000,and 10,000 lux),and an ultraviolet B(UVB)lighting group(302 nm,1,000μw/cm2).After 24 hours of continuous illumination,full-field flash electroretinogram(FERG)and pathological examination were performed in each group.Results:As revealed by FERG,the impairment of retinal function gradually worsened with the increase of LED light intensity.In contrast,the UVB group had the most severe retinal function impairment.Particularly,the functional damage of rod cells and inner nuclear layer cells was the main FERG finding in each group.In the NC group,the retina had typical morphologies featured by well-defined structures,clearly visible border between the inner and outer segments,and neatly arranged inner and outer nuclear layer cells.After 24 hours of illumination,the inner and outer parts of the retina in the 4,000 lux group were still neatly arranged,along with a clear border;however,the inner and outer nuclear layers were randomly arranged,and some irregular nuclei and cells were lost.The damage of the internal and external retinal segments and the internal and external nuclear layers became more evident in the 6,000 lux group,7,000 lux group,and 10,000 lux group.The UVB group had a more obviously disordered arrangement of inner and outer nuclear layers and loss of cells.Conclusions:Continuous exposure to white LED light can cause structural and functional damage to rat retinas,and such damage is related to the intensity of illumination.Therefore,the risk of retinal damage should be considered during LED illumination,and proper LED illumination intensity may help to maintain eye health. 展开更多
关键词 RETINA light damage light emitting diode(LED) ultraviolet B(UVB) rat
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The effect of the degree of left renal vein constriction on the development of adolescent varicocele in Sprague-Dawley rats 被引量:4
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作者 Bing Yao Wen-Liang Zhou +5 位作者 Da-Yu Han Bin Ouyan Xu Chen Sheng-Fu Chen Chun-Hua Deng Xiang-Zhou Sun 《Asian Journal of Andrology》 SCIE CAS CSCD 2016年第3期471-474,I0011,共5页
Experimental models have allowed inquiry into the pathophysiology of varicocele (VC) beyond that possible with human patients. A randomized controlled study in rats was designed to clarify the influence of the degre... Experimental models have allowed inquiry into the pathophysiology of varicocele (VC) beyond that possible with human patients. A randomized controlled study in rats was designed to clarify the influence of the degree of left renal vein constriction on the development of adolescent VC. Fifty adolescent male Sprague-Dawley rats (Rattus norvegicus) were randomly assigned to five groups of 10: the experimental groups (I-IV) underwent partial ligation of left renal veins with 0.5-, 0.6-, 0.7-, and 0.8-mm diameter needles, respectively. The control group (V) underwent a sham operation. The diameter of the left spermatic vein (LSV) was measured at baseline and 30 days postoperatively. In addition, the lesion of the left kidney was examined with the naked eye and assessed by Masson's trichrome staining. VC was successfully induced in 2 (20%), 4 (40%), 7 (70%), and 10 (100%) rats in groups I-IV, respectively. The other rats failed to develop VCs primarily due to left renal atrophy. No VC was observed in group V. The postsurgical LSV diameters in VC rats in groups III and IV were 1.54 ± 0. 16 and 1.49 ± 0.13 mm, respectively (P〉 0.05), and their increments were 1.36±0. 10 and 1.31±0. 10 ram, respectively (P 〉 0.05). These results suggest that suitable constriction of the left renal vein is critical for adolescent VC development. In addition, the 0.8-mm diameter needle may be more suitable for inducing left renal vein constriction in adolescent rat models. 展开更多
关键词 ADOLESCENT KIDNEY left renal vein constriction left spermatic vein sprague-dawley rat VARICOCELE
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Outer hair cells isolation from postnatal Sprague-Dawley rats
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作者 Mei-Hao Qi Yang Qiu +7 位作者 Ke-Yong Tian Kun Liang Hui-Min Chang Ren-Feng Wang Er-Fang Chen Wei-Long Wang Ding-Jun Zha Jian-Hua Qiu 《World Journal of Otorhinolaryngology-Head and Neck Surgery》 2019年第1期14-18,共5页
Outer hair cells (OHCs) damage is a general phenomenon in clinical disorders such as noise-induced hearing loss and drug-induced hearing loss.In order to elucidate the mechanism underlying these disorders,OHCs-its dis... Outer hair cells (OHCs) damage is a general phenomenon in clinical disorders such as noise-induced hearing loss and drug-induced hearing loss.In order to elucidate the mechanism underlying these disorders,OHCs-its diseased region needs to be deeply investigated.However,OHCs array on the basilar membrane which contains massive cells with different types.Therefore,to isolate OHCs from this huge population is significant for revealing its pathological and molecular changes during disease processing.In the present study,we tried to isolate OHCs from the commonly used animal model-Sprague-Dawley (SD) rats.By separating outer hair cells from SD rats with different day ages,we found that 9 days after birth was a suitable time for the separation of the OHCs.At this time,the number of OHCs isolated from rats was large,and the cell morphology was typical of cylindrical shape.OHCs isolated using this method are histologically suitable and quantitatively adequate for molecular biological and electrophysiological analyses. 展开更多
关键词 Outer HAIR cell ISOLATION technique MORPHOLOGY sprague-dawley rat
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Effect of ethanolic extract of Pericampylus glaucus (Lam) Merr on biochemical parameters in Sprague-Dawley rats with high fats diets
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作者 Kifayatullah Muhammad Mustapha Mohd Shahimi +3 位作者 Aravinth Vijay Jesuraj Sengupta Pinaki Khan Muhammad Shahid Kaleemullah 《Journal of Coastal Life Medicine》 CAS 2017年第5期219-223,共5页
Objective: To evaluate the effect of ethanolic extract of Pericampylus glaucus (Lam) Merr (P. glaucus) on biochemical parameters in high fats diets treated rats. Methods: The effect of P. glaucus extract on biochemica... Objective: To evaluate the effect of ethanolic extract of Pericampylus glaucus (Lam) Merr (P. glaucus) on biochemical parameters in high fats diets treated rats. Methods: The effect of P. glaucus extract on biochemical parameters was studied in male Sprague Dawley rats. The high-fat diets along with standard normal laboratory rat's diets were given for a period of 28 days along with ethanolic extract of P. glaucus and standard drug. The standard group was treated with simvastatin at dose of 80 mg/kg and the tested groups were treated with ethanol extract of P. glaucus at 400, 600 and 800 mg/kg daily for 28 days of the experiment. The weight of animals in each group was measured weekly and the effect of extract and standard on serum lipids profile was evaluated at the end of the study. Results: The results showed that the ethanolic extract of P. glaucus at different doses of 400, 600 and 800 mg/kg and standard drug induced a significant reduction in the body weight (P< 0.001), total cholesterol, triglycerides (TG), low density lipoproteins (LDL) and uric acid levels and increase (P < 0.001) in high density lipoproteins (HDL) as compared to untreated hyperlipidemic group. Conclusions: The present work indicates that the ethanolic extract significantly suppressed the biochemical parameters in high fats diet treated animals, suggesting the antihyperlipidemic potential of P. glaucus. 展开更多
关键词 Ethanolic EXTRACT Pericampylus glaucus (Lam) Merr BIOCHEMICAL parameters MALE sprague-dawley rats
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Inhibition of central Na+/H+ exchanger type 3 can alleviate sleep apnea in Sprague-Dawley rats
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作者 Wang Qimin Zhou Rong Zhang Cheng Dong Hui Ma Jing Wang Guangfa 《Chinese Medical Journal》 SCIE CAS CSCD 2014年第1期48-53,共6页
Background Recent studies showed the central Na+/H+ exchanger type 3 (NHE3) has a close relationship with ventilation control.The objective of the study is to investigate the role of NHE3 in sleep apnea in Sprague... Background Recent studies showed the central Na+/H+ exchanger type 3 (NHE3) has a close relationship with ventilation control.The objective of the study is to investigate the role of NHE3 in sleep apnea in Sprague-Dawley (SD) rats.Methods A sleep study was performed on 20 male SD rats to analyze the correlation between the sleep apneic events and total NHE3 protein content and inactive NHE3(pS552) in the brainstem measured by Western blotting.Another 20 adult male SD rats received 3 days of sleep and respiration monitoring for 6 hours a day,with adaption on the first day,0.5% DMSO microinjection into the fourth ventricle on the second day,and AVE0657 (specific inhibitor of NHE3) microinjection on the third day.Rats were divided into two groups with injection of 5 μmol/L or 8 μmol/L AVE0657 before the sleep study.The effects of AVE0657 on sleep apnea and sleep structure of rats were analyzed through self-control.Results The total post-sigh apnea index (TPSAI) and post-sigh apnea index in non-rapid eye movement (NREM) sleep (NPSAI) and total apnea index (AI) in NREM sleep (NAI) were negatively correlated with NHE3(pS552) protein contents in the brainstem (r=-0.534,-0.547 and-0.505,respectively,P<0.05).The spontaneous apnea index in REM sleep (RSPAI) was positively correlated with the level of NHE3(pS552) protein expression in the brainstem (r=0.556,P<0.05).However,the sleep AI had no relationship with total NHE3 protein.Compared with the blank control and microinjection of 0.5% DMSO,5 μmol/L AVE0657 significantly reduced the total AI and NPSAI (both P<0.05) without a significant effect on sleep architecture.In contrast to blank control and microinjection of 0.5% DMSO,injection of 8 μmol/L AVE0657 significantly reduced the AI and PSAI in NREM and REM sleep (all P<0.05).Conclusions The severity of sleep apnea was negatively correlated with central inactive NHE3.A specific inhibitor of NHE3 decreased the sleep AI.Thus,our results indicate that central NHE3 might be a molecular target for sleep apnea treatment,whose inhibitors may be potential therapeutic drugs for sleep apnea. 展开更多
关键词 Na+/H+ exchanger 3 rat sleep apnea sleep monitoring
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Emerging role of liquid biopsy in rat sarcoma virus mutated metastatic colorectal cancer:A case report 被引量:1
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作者 João Gramaça Isabel Gomes Fernandes +4 位作者 Carolina Trabulo Joana Gonçalves Rita Gameiro dos Santos Adriano Baptista Idília Pina 《World Journal of Gastrointestinal Oncology》 SCIE 2024年第1期234-243,共10页
BACKGROUND In patients with metastatic colorectal cancer(mCRC),the treatment options are limited and have been proved to be affected by rat sarcoma virus(RAS)mutational status.In RAS wild-type(wt)patients,the combinat... BACKGROUND In patients with metastatic colorectal cancer(mCRC),the treatment options are limited and have been proved to be affected by rat sarcoma virus(RAS)mutational status.In RAS wild-type(wt)patients,the combination of antiepidermal growth factor receptor(EGFR)monoclonal antibodies with chemotherapy(CT)is more effective than CT alone.On the other hand,RAS-mutated patients are not eligible for treatment with anti-EGFR antibodies.CASE SUMMARY Eleven patients with initially RAS-mutated mCRC were followed from diagnosis to May 2022.At the time of cell-free DNA determination,five patients had undergone one CT line,five patients had undergone two CT lines,and one patient had undergone three CT lines(all in combination with bevacizumab).At the second and third treatment lines[second line(2L),third line(3L)],patients with neo-RAS wt received a combination of CT and cetuximab.In neo-RAS wt patients treated with anti-EGFR,our findings indicated an increase in progression-free survival for both 2L and 3L(14.5 mo,P=0.119 and 3.9 mo,P=0.882,respectively).Regarding 2L overall survival,we registered a slight increase in neo-RAS wt patients treated with anti-EGFR(33.6 mo vs 32.4 mo,P=0.385).At data cut-off,two patients were still alive:A RAS-mutated patient undergoing 3L treatment and a neo-RAS wt patient who received 2L treatment with anti-EGFR(ongoing).CONCLUSION Our case series demonstrated that monitoring RAS mutations in mCRC by liquid biopsy may provide an additional treatment line for neo-RAS wt patients. 展开更多
关键词 Metastatic colorectal cancer rat sarcoma virus mutational status Liquid biopsy rat sarcoma virus wild-type Neo-rat sarcoma virus wild-type Anti-epidermal growth factor receptor therapy Case report
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Chemokine platelet factor 4 accelerates peripheral nerve regeneration by regulating Schwann cell activation and axon elongation 被引量:2
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作者 Miao Gu Xiao Cheng +3 位作者 Di Zhang Weiyan Wu Yi Cao Jianghong He 《Neural Regeneration Research》 SCIE CAS CSCD 2024年第1期190-195,共6页
Schwann cells in peripheral nerves react to traumatic nerve injury by attempting to grow and regenerate.Howeve r,it is unclear what factors play a role in this process.In this study,we searched a GEO database and foun... Schwann cells in peripheral nerves react to traumatic nerve injury by attempting to grow and regenerate.Howeve r,it is unclear what factors play a role in this process.In this study,we searched a GEO database and found that expression of platelet factor 4 was markedly up-regulated after sciatic nerve injury.Platelet factor is an important molecule in cell apoptosis,diffe rentiation,survival,and proliferation.Further,polymerase chain reaction and immunohistochemical staining confirmed the change in platelet factor 4 in the sciatic nerve at different time points after injury.Enzyme-linked immunosorbent assay confirmed that platelet factor 4 was secreted by Schwann cells.We also found that silencing platelet factor 4 decreased the proliferation and migration of primary cultured Schwann cells,while exogenously applied platelet factor 4 stimulated Schwann cell prolife ration and migration and neuronal axon growth.Furthermore,knocking out platelet factor 4 inhibited the prolife ration of Schwann cells in injured rat sciatic nerve.These findings suggest that Schwann cell-secreted platelet factor 4 may facilitate peripheral nerve repair and regeneration by regulating Schwann cell activation and axon growth.Thus,platelet factor 4 may be a potential therapeutic target for traumatic peripheral nerve injury. 展开更多
关键词 axon elongation bioinformatic analysis cell migration cell proliferation dorsal root ganglia peripheral nerve regeneration peripheral nerve trauma platelet factor 4 rat sciatic nerve Schwann cells
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Anti-Obesity Effects of Dietary d-Allulose and Medium-Chain Triglycerides in High-Fat Diet-Fed Rats
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作者 Tatsuhiro Matsuo Chihiro Yokoyama +4 位作者 Takako Yamada Tetsuo Iida Susumu Mochizuki Akihide Yoshihara Kazuya Akimitsu 《Food and Nutrition Sciences》 CAS 2024年第8期701-710,共10页
d-Allulose, a rare sugar, exerts anti-obesity effects by inhibiting hepatic lipogenesis and promoting energy expenditure. Medium-chain triglycerides (MCTs) consist of three medium-chain fatty acids connected by glycer... d-Allulose, a rare sugar, exerts anti-obesity effects by inhibiting hepatic lipogenesis and promoting energy expenditure. Medium-chain triglycerides (MCTs) consist of three medium-chain fatty acids connected by glycerol. MCTs have been extensively investigated for their ability to reduce body fat accumulation. We previously investigated the anti-obesity effects of a combination of dietary d-allulose and MCT (5% - 13%) in rats;however, we could not confirm the anti-obesity effects of MCT or observed synergetic effects between d-allulose and MCT on body fat loss. We speculated that our previous studies were influenced by the excessive amount of MCT in the diets. Therefore, in this study, we aimed to investigate the anti-obesity effects of the simultaneous intake of d-allulose and MCT in rats fed an obesity-inducing high-fat diet with a low amount of MCTs (2%). Thirty-two male Wistar rats (3-week-old) were randomly divided into four groups: control, d-allulose, MCT, and d-allulose + MCT groups. Rats in each group were fed ad libitum on a control (no d-Allulose or MCT), 5% d-allulose, 2% MCT, or 5% d-allulose + 2% MCT diets for 16 weeks. Abdominal adipose tissue weights were significantly lower in the d-allulose diet group than in the control group, whereas no differences were observed between results of the MCT-supplemented groups. The total body fat mass was significantly lower in the d-allulose and MCT diet groups than in the control group, but no differences were observed between the MCT-supplemented groups. These results suggested that anti-obesity effects of dietary d-allulose were observed, and the effects of dietary MCTs were weaker than those of d-allulose. Moreover, we confirmed the interaction between dietary d-allulose and MCT on indicators of obesity. Interestingly, their effects were not synergistic, as MCT supplementation offset the anti-obesity effects of dietary d-allulose. However, the specific mechanisms underlying those effects remain unknown, warranting further investigation. 展开更多
关键词 Rare Sugar d-Allulose Medium-Chain Triglycerides Body Fat rat
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Probiotic microorganisms affect the reproductive and nervous systems of male rats treated with acrylamide
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作者 Seyedhossein Hekmatimoghaddam Maryam Yadegari +2 位作者 Fateme Zare Fatemeh Zakizadeh Seyed Mohammad Seifati 《Asian pacific Journal of Reproduction》 CAS 2024年第4期178-186,共9页
Objective:To evaluate the protective effects of probiotic microorganisms on the reproductive and nervous systems of male rats treated with acrylamide.Methods:Thirty-two rats were randomly divided into 4 groups and rec... Objective:To evaluate the protective effects of probiotic microorganisms on the reproductive and nervous systems of male rats treated with acrylamide.Methods:Thirty-two rats were randomly divided into 4 groups and received normal saline through gavage(control),acrylamide 20 mg/kg body weight,acrylamide plus probiotic microorganisms(Lactobacillus acidophilus,Lactobacillus casei,Lactobacillus bulgaricus,Lactobacillus rhamnosus,Bifidobacterium breve,Bifidobacterium infantis,Streptococcus thermophilus and fructooligosaccharides,all mixed in sachets)20 or 200 mg/kg body weight,respectively.After 30 days,the testis,prostate,seminal vesicle and cerebellum were removed,fixed and stained with hematoxylin-eosin(H&E).The Johnsen score was used to classify spermatogenesis.Cavalieri's principle method was used to evaluate the total volume(in mm3)of the testes.The number of each intratubular cell type as well as intertubular Leydig cells in whole samples was measured using the physical dissector counting techniques.Stereological analysis and the grids were used to determine the volume of cerebellar layers as well as the Purkinje cell number.Results:The testis weight decreased significantly in the acrylamide-treated group compared to the other groups(P<0.001).The number of spermatogonia,spermatocytes,spermatids and Leydig cells in the acrylamide-treated group were significantly less compared to the control group(P<0.05),while they were increased significantly in the acrylamide+200 mg/kg probiotic group(P<0.05;P<0.01).The mean Johnsen score in the acrylamide-treated group was lower than in the control group(P<0.001).Acrylamide-induced changes including congestion,vacuolization in the secretory epithelial cells,and epithelial rupture were observed in the prostate and seminal vesicle.The volumes of cerebellar layers were decreased in the acrylamide group compared to the control group while recovered in both probiotic treated groups.Conclusions:Probiotic microorganisms alleviate acrylamide-induced toxicities against the reproductive and cerebellar tissues in rats. 展开更多
关键词 ACRYLAMIDE CEREBELLUM MICROORGANISMS PROBIOTICS Prostate rats TESTIS
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Effects of heat stress and long photoperiod on the prostate of rats
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作者 Hamid Reza Ghaffari Javad Poursamimi 《Asian pacific Journal of Reproduction》 CAS 2024年第4期187-196,共10页
Objective:To examine light and heat effects on the morphological,histological,and micrometric structure of the prostate of rats.Methods:Thirty adult male rats were divided into three groups.The control group was kept ... Objective:To examine light and heat effects on the morphological,histological,and micrometric structure of the prostate of rats.Methods:Thirty adult male rats were divided into three groups.The control group was kept under 20℃-22℃ and an artificial 12 h/12 h day/night cycle;the temperature group was under normal light and at(42±1)℃ heat for 4 to 5 h daily,and the light group was exposed to 8 h/16 h day/night cycle with 20℃-22℃.Rats were weighed five times(at the beginning of the study and every seven days).Five milliliters(mL)of their peripheral blood were taken.The tissue staining was performed using the hematoxylin-eosin(H&E)stain and periodic acid-Schiff(PAS).In the following,tissue and cellular reactions to the PAS were examined.Results:Folds were located entirely on the surface of the anterior lobe and periphery of the other lobes.The secretory units in the anterior lobe were more than the lateral lobe.A strong reaction of the secretory cells to the PAS was observed.Testosterone serum levels of the light group also significantly increased compared to the control group(P<0.05).The most histometric changes of the lobes were established in the lateral lobes.Heat stress resulted in a significant decrease in testosterone levels and transformed prostate tissue.The epithelium and parenchyma to scaffold ratio in the temperature group decreased.Conclusions:Maximum and minimum changes in the ventral lobe happened under the ascent of temperature and light,respectively.The ventral lobe in the study of prostatic hyperplasia should be more considered. 展开更多
关键词 PROSTATE Heat stress PHOTOPERIOD HISTOLOGY TESTOSTERONE rat
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Hydrogel dressings on neurotrophic keratitis in an experimental animal model
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作者 Hua-Qin Xia Xiao-Dan Jiang +2 位作者 Yi-Fan Song Xue-Min Li Yan-Jie Tian 《International Journal of Ophthalmology(English edition)》 SCIE CAS 2024年第8期1396-1402,共7页
AIM:To investigate the therapeutic effects of hydrogel dressings on neurotrophic keratitis in rats.METHODS:Male Wistar rats,aged 42–56d,were randomly divided into control,experimental,and treatment groups,each consis... AIM:To investigate the therapeutic effects of hydrogel dressings on neurotrophic keratitis in rats.METHODS:Male Wistar rats,aged 42–56d,were randomly divided into control,experimental,and treatment groups,each consisting of five rats.The experimental and treatment groups underwent neurotrophic keratitis modeling in both eyes.After successful modeling,biomedical hydrogels formed with polyvinyl alcohol and polyvinyl pyrrolidone were used in treatment group for 7d.Ocular irritation response and keratitis index scores,Schirmer’s test,tear film break-up time(BUT),sodium fluorescein staining,and hematoxylin and eosin(HE)staining were used to evaluate the effectiveness of the treatment.RESULTS:The neurotrophic keratitis model was successfully established in rats with severe ophthalmic nerve injury,characterized by keratitis,ocular irritation,reduced tear secretion measured by decreased BUT and Schirmer test values,corneal epithelial loss,and disorganized collagen fibers in the stromal layer.Following treatment with hydrogel dressings,significant improvements were observed in keratitis scores and ocular irritation symptoms in model eyes.Although the recovery of tear secretion,as measured by the Schirmer’s test,did not show statistical differences,BUT was significantly prolonged.Fluorescein staining confirmed a reduction in the extent of corneal epithelial loss after treatment.HE staining revealed the restoration of the structural disorder in both the epithelial and stromal layers to a certain extent.CONCLUSION:Hydrogel dressing reduces ocular surface irritation,improves tear film stability,and promotes the repair and restoration of damaged epithelial cells by maintaining a moist and clean environment on the ocular surface in the rat model. 展开更多
关键词 neurotrophic keratitis HYDROGEL corneal epithelial cells rat
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