Establishment of a diatom-total phosphorus transfer function for lakes on the Songnen Plain in northeast China

To understand the relationship between planktonic diatoms and environmental variables in the lakes on the Songnen Plain,northeast(NE)China,we investigated water quality and planktonic diatoms from 71 sampling points in 27 lakes,based on which Canonical Correspondence Analysis(CCA)was conducted.The result show that planktonic diatoms displayed certain responses to environment gradients,and the total phosphorus(TP)explained the maximum variation of planktonic diatom species among the 15 environmental variables,suggesting that TP was the most important and significant environmental parameter affecting the distribution of diatom species.In addition,we established a diatom-total phosphorus transfer(DTPT)function,of which component 5 of the weighted averaging partial least squares regression(WAPLS)was chosen to and compared with a series of weighted average regression(WA)models and WA-PLS models.According to the jackknifing statistical test,the component 5 of WA-PLS models provided a lower root-mean-square error prediction(RMSEP=0.202)and a higher correlation coefficient between observation and prediction(R^2_Jack=0.759).After deletion of three outliers,the root-mean-square error prediction of the DTPT function was reduced(RMSEP=0.169)while the correlation coefficient between observation and prediction was increased(R^2_Jack=0.823).Therefore,this DTPT function performed better than other regional TP models in the world.However,it remains demanding for expanding the background dataset to improve the prediction ability of the model.

A data-derived soft-sensor method for monitoring effluent total phosphorus

The effluent total phosphorus(ETP) is an important parameter to evaluate the performance of wastewater treatment process(WWTP). In this study, a novel method, using a data-derived soft-sensor method, is proposed to obtain the reliable values of ETP online. First, a partial least square(PLS) method is introduced to select the related secondary variables of ETP based on the experimental data. Second, a radial basis function neural network(RBFNN) is developed to identify the relationship between the related secondary variables and ETP. This RBFNN easily optimizes the model parameters to improve the generalization ability of the soft-sensor. Finally, a monitoring system, based on the above PLS and RBFNN, named PLS-RBFNN-based soft-sensor system, is developed and tested in a real WWTP. Experimental results show that the proposed monitoring system can obtain the values of ETP online and own better predicting performance than some existing methods.

Continuous flow analysis of dissolved total phosphorus in seawater by UVK_(2)S_(2)O_(8) online digestion method

Several methods for analysis of dissolved total phosphorus in seawater were reviewed. Discussions were focused on UVirradiation and persulphate oxidation methods which are the most popular dissolved organic phosphorus determinationmethods presently. The compounds used for the phosphorus recovery test were categorized into three groups accordingto their chemical structure. It was found that low power UV irradiation can decompose POC or PC bonds efficientlybut may be inefficient for POP bonds. Heating-bath in acid condition is useful for decomposing POP bonds. Usingthe continuous flow analysis system (Auto-analyzer II), UV digestion and heating-bath, series experiments were carriedout based on the above analysis. Eleven model compounds were employed for the phosphorus recovery test and thefactors influencing the decomposition efficiency of dissolved compounds containing phosphorus were clarified. Finally,the optimal design for determination of dissolved total phosphorus in seawater based on the routine continuous flowanalysis system was presented. For the organic mono-phosphate, the recovery is more than 90% and a recovery of33%~51% was obtained for inorganic or organic polyphosphates. Up to now, this is the highest decompositionefficiency for dissolved phosphorus based on the continuous flow analysis system.

Advances in Detection of Total Phosphorus and Application of Titanium Dioxide Photocatalytic Technology

Total phosphorus is an important index in the monitoring of water quality, but the existing detection methods waste time and energy during the process of di- gestion. In this paper, the existing detection technology of total phosphorus and the digestion technology of organic phosphorus in the determination of total phosphorus were summarized firstly, of which photocatalytic degradation technology, as one of new technologies of organic phosphorus digestion, was introduced. Afterwards, tita- nium dioxide photocatalytic technology and its application were summarized, and its application prospects were expected.

Effects of Conservation Methods of Water Samples on Determination of Total Phosphorus

Effects of conservation methods of water samples on determination of total phosphorus were analyzed from aspects of methods of adding conserving agent, SS concentration, natural sedimentation time, conservation temperature and time. The results showed that SS concentration in water and natural sedimentation time had the greatest impact on the determination of total phosphorus, while the influence of methods of adding conserving agent, conservation temperature and time was relatively smaller. TP concentration in water samples conserved irregularly was higher than the standard value due to the existence of SS and microorganisms in water, and the deviation became larger with the increase of SS concentration in water. In addition, quality control measures and matters needing attention during the conservation process of water samples were put forward to improve the accuracy of TP concentration.

Effects of Sediment on the Test of Total Phosphorus in Water

Based on previous studies,the effects of the type,content,particle size and state of sediment on the determination of total phosphorus in water are analyzed.The results show that the type of sediment has a certain influence on the determination result of total phosphorus,and the influence cannot be ignored.The degree of influence depends on the specific type.Sediment content is negatively correlated with the determination result of total phosphorus in water.That is,the higher the sediment content is,the lower the determination result is.The particle size of sediment is positively correlated with the determination result of total phosphorus in water.That is,the larger the particle size of sediment is,the higher the determination result is.Among the static,semi-suspended and completely suspended states,the sediment in the static state has the least impact on the determination of total phosphorus,and it has the greatest impact on the determination of total phosphorus when it is fully suspended.Therefore,in the analysis and testing process of total phosphorus,attention should be paid to the impact of sediment to improve the authenticity of the test results.

TOTAL PHOSPHORUS RELEASE FROM BOTTOM SEDIMENTS IN FLOWING WATER

In this paper, the bottom of the Dianshan Lake was selected as a test sample. The dynamic release of contaminated sediments into the overlying water column was experimentally investigated in an open water channel under different hydrodynamic conditions. The experimental results indicate that the Total Phosphorus （TP） release process can be divided into three stages： rapid release, slow release and equilibration release. In the initial release stage the measured TP concentration changes along the depth. The TP concentration near the sediment-water interface is higher than that near the water surface, but the TP concentration becomes uniform along the depth after 3 h. The dynamic release of re-suspension sediment pollutants is about 6 times higher than the static release of sediment-water interface. There are three main types of release mechanism： diffusion release, re-suspended pore water mixing release and re-suspended particles desorbing release.

Total phosphorus accident pollution and emergency response study based on geographic information system in Three Gorges Reservoir area

In recent years,sudden water pollution accidents in China's rivers have become more frequent,resulting in considerable effects on environmental safety.Therefore,it is necessary to simulate and predict pollution accidents.Simulation and prediction provide strong support for emergency disposal and disaster reduction.This paper describes a new two-dimensional water quantity and the quality model that incorporates a digital elevation model into the geographic information system.The model is used to simulate sudden water pollution accidents in the main stream of the Yangtze River and Jialing River in the Chongqing section of the Three Gorges Reservoir area.The sectional velocity distribution and concentration change of total phosphorus are then analyzed under four hydrological situations.The results show that the proposed model accurately simulates and predicts the concentration change and migration process of total phosphorus under sudden water pollution accidents.The speed of migration and diffusion of pollutants is found to be greatest in the flood season,followed by the water storage period,drawdown season,and dry season,in that order.The selection of an appropriate water scheduling scheme can reduce the peak concentration of river pollutants.This study enables the impact of pollutants on the ecological environment of river water to be alleviated,and provides a scientific basis for the emergency response to sudden water pollution accidents in the Three Gorges Reservoir area.

Effects of Phosphorus Concentration on the Treatment of Domestic Wastewater by a Combination of Hydrolytic Acidification and Biological Aerated Filter Filled with Mussel Shells

The combination of hydrolytic acidification and biological aerated filter （BAF） filled with mussel shells was used to treat domestic wastewater, and the removal rates of chemical oxygen demand （COD）, ammonia nitrogen （NH3-N） and total phosphorus （TP） by the system were analyzed under different TP concentrations. When TP concentration ranged from 12.39 to 14.69 mg/L, the removal rate of COD was the best, over 90.92% ; as TP concentration varied from 2.26 to 2.61 mg/L, the removal rates of NH3-N and TP were the best, up to 100.00% and 76.38% respectively. The results show that it is feasible to use mussel shells as the media of BAF, and TP concentration has certain influence on the performance of the system dealing with domestic wastewater.

Identifying Nonpoint Sources of Phosphorus and Nitrogen: A Case Study of Pollution That Enters a Freshwater Wetland (Laguna Cartagena, Puerto Rico)

Point and nonpoint sources of phosphorus (P) and nitrogen (N) can cause reductions in water quality, including eutrophication. Nonpoint pollution represents a special challenge because of dispersed not easily identifiable sources such as the runoff from soil, nutrients, and other chemicals from agricultural fields and residential areas. Laguna Cartagena is a tropical freshwater wetland, situated in southwestern Puerto Rico. It is a eutrophic ecosystem, and its eutrophication is caused by both external nutrient loading and internal, mainly by phosphorus. This wetland has been affected by phosphorus loading from inorganic agricultural fertilizer in this historically oligotrophic wetland system until the end of subsidized fertilizer use and sugar cane cultivation in the late 1990s. This study identifies: 1) nonpoint sources of phosphorus (SRP, Soluble Reactive Phosphorus and TP, Total Phosphorus) and nitrogen (nitrate, nitrite, and ammonia) that enter Laguna Cartagena;and 2) the role of precipitation events on the contributions of phosphorus and nitrogen loading to ecosystems. Herein we assess water samples from five channelized external sources of P and N that enter Laguna Cartagena at two-week intervals from October 2013 through November 2014. Rainfall data were obtained weekly from a rain gauge. Standard methods were used for all chemical analyses. Results showed that the channelized waterways that carry water to the lagoon can be classified as hypereutrophic (>100 μg/L) for TP concentrations and oligotrophic (<200 μg/L) for nitrogen concentrations. Currently agriculture (rice and cattle) is the predominant land use at the nearby University of Puerto Rico (UPR) Lajas Agricultural Experiment Substation, the predominant nonpoint source of nutrient pollution (SRP, TP and ammonia) in the principal channelized water sources to the lagoon. Current nutrient loads are likely derived from fertilizers applied to the Substation’s rice fields, and a high density livestock. The second important cause of external surface water degradation (SRP, TP and ammonia) is the discharge from rural households in the drainage basin that discharge greywater directly to the environment, as indicated by the results from Cerro Alto hills immediately to the north of the lagoon. Precipitation also was associated with SRP, TP and ammonia loads.

Effects of Long-term Located Fertilization on Evolution of Available Phosphorus and Phosphorus Pool in Shandong Fluvo-aquic Soil

This study was conducted to investigate the effects of long-term located fertilization on soil phosphorus,the changes of soil available phosphorus(OlsenP),the evolution of soil total phosphorus(TP)and the ratio change of Olsen-P to TP(PAC)by 33-year fertilization experiments in winter wheat-summer maize rotation system in Shandong fluvo-aquic soil.Eight treatments were designed as no fertilization(CK),nitrogen fertilizer(N),nitrogen and phosphate fertilizer(NP),nitrogen and potassium fertilizer(NK),phosphate and potassium fertilizer(PK),nitrogen-phosphate-potassium fertilizer(NPK),reduced NPK fertilizer(N(15)PK),and increased NPK fertilizer(N_(25)PK).Meanwhile,eight organic fertilizer-added treatments were designed based on the application of inorganic fertilizer the same as the above ones.The results showed that TP,Olsen-P and PAC of treatments added with organic fertilizer were higher than those without organic fertilizer,and those of the treatments applied with phosphate fertilizer were higher than those of no phosphate fertilizer.With the increase of years,soil P pool decreased due to crop absorption,nutrient loss and morphological transformation and other causes under the treatments of without and only phosphate fertilizer,while remained stable under the treatments added with organic fertilizer.The PAC values were generally lower in fluvo-aquic soil,and it could be improved by the application of organic fertilizer.On the whole,the application of chemical phosphate fertilizer combined with organic fertilizer could improve the phosphorus content in soil and ensure the supply of phosphorus nutrition.This study would provide scientific basis for fertilization management and soil fertility in fluvo-aquic soil.

Inversion of Water Quality TN-TP Values Based on Hyperspectral Features and Model Validation

Using hyperspectral data collected in January and June 2022 from the Sha River,the concentrations of total nitrogen(TN)and total phosphorus(TP)were estimated using the differential method.The results indicate that the optimal bands for estimation vary monthly due to temperature fluctuations.In the TN model,the power function model at 586 nm in January exhibited the strongest fit,yielding a fit coefficient(R2)of 0.95 and F-value of 164.57 at a significance level(p)of less than 0.01.Conversely,the exponential model at 477 nm in June provided the best fit,with R2=0.93 and F=80.95 at p<0.01.In the TP model,the exponential model fit of the differential values at 851 nm with TP in January produced the best results,with R2=0.78 and F=20.61.However,the overall fit in June outperformed that in January.Specifically,the quadratic and linear model fits of the differential values at 824 and 863 nm with TP achieved R2=0.96 and F-values of 34.42 and 203.34,respectively.

Correlation Analysis between Abundance of Trichodina spp. and Water Quality Parameters

To understand the correlations between the abundance of Trichodina spp. and the water quality parameters, the abundance of Trichodina spp. on the gill of yellow catfish in aquaculture water was investigated. Meanwhile, the temperature and total phosphorus, available phosphorus, total nitrogen, nitrite and ammonia nitro- gen contents of the aquaculture water were measured and determined. The results showed that the abundance of Trichodina spp. on the gill of yellow catfish was negatively correlated with the total phosphorus, available phosphorus and total nitro- gen contents in the aquaculture water, and was positively correlated with the nitrite content in the aquaculture water （P〈0.05）; there were no significant correlations between the abundance of Trichodina spp. and the temperature and ammonia nitrogen content of the aquaculture water （P〉0.05）.

Study on the Application of Phoslock in Purifying Eutrophic Water in Dianchi Lake

As a modified product,Phoslock can purify soluble phosphate(SP) in water to control water eutrophication effectively.The results showed that Phoslock had better removal effect for SP in the water of Dianchi Lake and could control water eutrophication effectively.Phoslock not only reduced SP concentration in water but also kept it at a lower level for a long time.Eutrophic water of Dianchi Lake treated by Phoslock had higher transparency,and the concentration of SP and total phosphorus(TP) was lower,with stronger anti-pollution ability to domestic sewage and agricultural non-point pollution.Therefore,Phoslock was a new dephosphorization reagent used in sewage and was safe to natural waters and aquatic organisms.

Soil Organic Carbon and Nutrients along an Alpine Grassland Transect across Northern Tibet

Soil carbon and nutrient contents and their importance in advancing our understanding of biogeochemical cycling in terrestrial ecosystem, has motivated ecologists to find their spatial patterns in various geographical area. Few studies have focused on changes in the physical and chemical properties of soils at high altitudes. Our aim was to identify the spatial distribution of soil physical and chemical properties in cold and arid climatic region. We also tried to explore relationship between soil organic carbon （SOC） and total nitrogen （TN）, total phosphorus （TP）, available nitrogen （AN）, available phosphorus （AP）, soil particle size distribution （PSD）. Samples were collected at 44 sites along a 300 km transect across the alpine grassland of northern Tibet. The study results showed that grassland type was the main factor influencing SOC, TN and TP distribution along the Gangdise Mountain-Shenzha-Shuanghu Transect. SOC, TN and TP contents were significantly higher in alpine meadow than alpine steppe ecosystems. SOC, TN, TP and AN contents in two soil layers （0-15 cm and 15-3o cm） showed no significant differences, while AP content in top soft （0-15 cm） was significantly higher than that in sub-top soil （15-30cm）. SOC content was correlated positively with TN and TP content （r = 0.901and 0.510, respectively）. No correlations were detected for clay content and fractal dimension of particle size distribution （D）. Our study results indicated the effects of vegetation on soil C, N and P seem to be more important than that of rocks itself along latitude gradient on the northern Tibetan Plateau. However, we did not found similar impacts of vegetation on soil properties in depth. Inaddition, this study also provided an interesting contribution to the global data pool on soil carbon stocks.

Variation in Enzymes Activities of Rhizospheric Substrate and Influencing Factors during Nursing of Watermelon Seedlings

[Objective] This study aimed to investigate the effects of substrate en- zymes activities on nursing of watermelon seedlings. [Method] The composted mushroom residue was mixed with garden soil according to a certain proportion to prepare nursing substrate for watermelon seedlings. During the nursing, the activity variation in alkaline phosphatase, acid phosphatase, neutral phosphatase and urease was investigated. In addition, the correlations between pH value, total nitrogen con- tent, total phosphorus content and organic matter content in substrate and enzyme activity were studied. At different young seedling stages, the rhizospheric substrates with different formulas were sampled for determination of enzymes activities. [Result] The enzyme activity differed significantly among different substrates. The correlation analysis results showed that the higher the organic matter content and total nitrogen content in substrate are, the higher the urease activity is; the phosphatase activity was significantly related to the organic matter content, total nitrogen content and to- tal phosphorus content in substrate; the pH value of substrate was significantly relat- ed to rhizospheric alkaline phosphatase activity; the shoot dry weight was positively related to urease activity; there was a significant correlation between phosphatase activity and root dry weight. [Conclusion] Through determining enzymes activities in the rhizospheric substrate for nursing watermelon seedlings, the optimum substrate M3 was screened out. The activities of rhizospheric urease, alkaline phosphatase, acid phosphatase and neutral phosphatase in the substrate M3 were all higher than those in the substrate fertilized with manure.

Effects of recovery time after fire and fire severity on stand structure and soil of larch forest in the Kanas National Nature Reserve, Northwest China

Forest recovery may be influenced by several factors, of which fire is the most critical.However, moderate-and long-term effects of fire on forest recovery are less researched in Northwest China.Thus, the effects of different forest recovery time after fire(1917(served as the control), 1974, 1983 and 1995) and fire severities(low, moderate and high) on larch(Larix sibirica Ledeb.) forest were investigated in the Kanas National Nature Reserve(KNNR), Northwest China in 2017.This paper analyzed post-fire changes in stand density, total basal area(TBA), litter mass, soil organic carbon(SOC) and soil nutrients(total nitrogen, total phosphorus and total potassium) with one-way analyses of variance.Results indicate that litter mass, TBA, SOC and soil nutrients increased with increasing recovery time after fire and decreasing fire severity, while the stand density showed an opposite response.The effects of fire disturbance on SOC and soil nutrients decreased with increasing soil depth.Moreover, we found that the time of more than 43 a is needed to recover the litter mass, TBA, SOC and soil nutrients to the pre-fire level.In conclusion, high-severity fire caused the greatest variations in stand structure and soil of larch forest, and low-severity fire was more advantageous for post-fire forest stand structure and soil recovery in the KNNR.Therefore, low-severity fire can be an efficient management mean through reducing the accumulation of forest floor fuel of post-fire forests in the KNNR, Northwest China.

Effects of Wetland Reclamation on Soil Nutrient Losses and Reserves in Sanjiang Plain,Northeast China

The carbon （C）, nitrogen （N） and phosphorus （P） variations of a temperate wetland soil under continuous cultivation for 40 yr were determined and evaluated in the Sanjiang Plain, Northeast China. The results showed that the soil organic carbon （SOC） and total nitrogen （TN） contents in each soil layer decreased sharply after cultivation for 2-3 yr, and exhibited minor differences after cultivation for 11 yr, which showed an exponential decline curve with the increase of cultivation years. The reduction rates of carbon and nitrogen reserves were 14.79% and 28.53% yr^-1 at the initial reclamation stages of 2-3 yr and then decreased to 2.02-3.08% yr^-1 and 1.98-2.93% yr^-1 after cultivation for 20 yr, respectively. Soil total phosphorus （TP） reserves decreased within cultivation for 5 yr, and then gradually restored to the initial level after cultivation for 17 yr. Both SOC and TN could be restored slightly when the farmland was left fallow for 8 yr after reclamation for 11 yr, whereas TP had no significant difference. These results demonstrated that wetland cultivation was one of the most important factors influencing on the nutrient fate and reserves in soil, which could lead to the rapid nutrient release and slow restoration through abandon cultivation, therefore protective cultivation techniques preventing nutrients from loss should be immediately established after wetland reclamation.

Impact of nutrient losses from agricultural lands on nutrient stocks in Dianshan Lake in Shanghai, China

Abstract： The water quality of Dianshan Lake in Shanghai Municipality, China, is impacted by nutrient losses from agricultural lands around the lake. In this study, nine types of agricultural land use were monitored in 2010 and 2011, and a correlation analysis between nutrient losses from agricultural non-point sources （NPS） and nutrient stocks in the lake was conducted over monthly and seasonal time periods. The results indicate that the monthly average concentration of total nitrogen （TN） ranged from 1.41 to 7.34 mg/L in 2010 and from 1.52 to 5.90 mg/L in 2011, while the monthly average concentration of total phosphorous （TP） ranged from 0.11 to 0.26 mg/L in 2010 and from 0.13 to 0.30 mg/L in 2011. The annual loss of TN from agricultural NPS was 195.55 tons in 2010 and 208.40 tons in 2011. The cultivation of water oat made the largest contribution to the loss of TN. The annual loss of TP was 44.58 tons in 2010 and 48.12 tons in 2011, and multi-vegetable cultivation made the largest contribution to the loss of TP. The results of correlation analysis show that the monthly stocks of TN and TP in the lake have a positive correlation with the monthly losses of TN and TP from agricultural NPS. According to the seasonal data, the stocks of TN and TP in the lake both have a much stronger correlation with the losses of TN and TP from agricultural NPS in summer than in other seasons. Agricultural NPS pollution control should be the main focus for the water resource conservation in this area.

Inference of Reference Conditions for Nutrient Concentrations of Chaohu Lake Based on Model Extrapolation

In the mid-eastern China,there are few or no lakes which are in the absence of anthropogenic disturbances,or their sediments remain undisturbed.As a result,the reference lakes distribution and paleolimnological reconstruction approaches usually are inappropriate to estimate lake reference conditions for nutrients.This yields the necessity of using the extrapolation methods to estimate the lake reference conditions for nutrients within those regions.The lake reference conditions for nutrients could be inferred inversely from the law of mass conservation,current lake nutrient concentration,and the loadings from watershed.Considering the scarcity of hydrological and water quality data associated with lakes and watersheds in China,as well as the low requirement of the watershed nutrient loadings models for these data,the soil conservation service(SCS) distributed hydrological model and the universal soil loss equation(USLE) were applied.The SCS model simulates the runoff process of the watershed,thereby calculating dissolved nutrients annually.The USLE estimates the soil erosion and particulate nutrients annually in a watershed.Then,with the loadings from atmospheric deposition and point source,the previous annual average nutrient concentrations could be acquired given the current nutrient concentrations in a lake.Therefore,the nutrient reference conditions minimally impacted by human activities could be estimated.Based on the proposed model,the reference conditions for total nitrogen and total phosphorus of Chaohu Lake,Anhui Province,China are 0.031 mg/L and 0.640 mg/L,respectively.The proposed reference conditions estimation model is of clear physical concept,and less data required.Thus,the proposed approach can be used in other lakes with similar circumstances.