Potato absorption and phytoavailability of Cd, Ni, Cu, Zn and Pb in sierozem soils amended with municipal sludge compost

Effects of sludge utilization on the mobility and phytoavailability of heavy metals in soil-plant systems have attracted broad attention in recent years. In this study, we analyzed the effects of municipal sludge compost （MSC） on the solubility and pIant uptake of Cd, Ni, Cu, Zn and Pb in a soil-potato system to explore the mobility, potato plant uptake and enrichment of these five heavy metals in sierozem soils amended with MSC through a potato cultivation trial in Lanzhou University of China in 2014. Ridge regression analysis was conducted to investigate the phytoavailability of heavy metals in amended soils. Furthermore, CaC12, CH3COONH4, CH3COOH, diethylene triamine pentacetic acid （DTPA） and ethylene diamJne tetraacetic acid （EDTA） were used to extract the labile fraction of heavy metals from the amended soils. The results show that the MSC could not only improve the fertility but also increase the dissolved organic carbon （DOC） content of sierozem soils. The total concentrations and labile fraction proportions of heavy metals increase with increasing MSC percentage in sierozem soils. In amended soils, Cd has the highest solubility and mobility while Ni has the lowest solubility and mobility among the five heavy metals. The MSC increases the concentrations of heavy metals in the root, stem, peel and tuber of the potato plant, with the concentrations being much higher in the stem and root than in the peel and tuber. Among the five heavy metals, the bioconcentration factor value of Cd is the highest, while that of Ni is the lowest. The complexing agent （DTPA and EDTA） extractable fractions of heavy metals are the highest in terms of phytoavailability. Soil properties （including organic matter, pH and DOC） have important impacts on the phytoavailability of heavy metals. Our results suggest that in soil-potato systems, although the MSC may improve soil fertility, it can also increase the risk of soils exposed to heavy metals.

Frequency Domain Filtering SAR Interferometric Phase Noise Using the Amended Matrix Pencil Model

Interferometric phase filtering is one of the key steps in interferometricsynthetic aperture radar (InSAR/SAR). However, the ideal filtering results are difficult toobtain due to dense fringe and low coherence regions. Moreover, the InSAR/SAR datarange is relatively large, so the efficiency of interferential phase filtering is one of themajor problems. In this letter, we proposed an interferometric phase filtering methodbased on an amended matrix pencil and linear window mean filter. The combination ofthe matrix pencil and the linear mean filter are introduced to the interferometric phasefiltering for the first time. First, the interferometric signal is analyzed, and theinterferometric phase filtering is transformed into a local frequency estimation problem.Then, the local frequency is estimated using an amended matrix pencil at a window. Thelocal frequency can represent terrain changes, thus suggesting that the frequency can beaccurately estimated even in dense fringe regions. Finally, the local frequency is filteredby using a linear window mean filter, and the filtered phase is recovered. The proposedmethod is calculated by some matrices. Therefore, the computational complexity isreduced, and the efficiency of the interferometric phase filtering is improved.Experiments are conducted with simulated and real InSAR data. The proposed methodexhibits a better filtering effect and an ideal efficiency as compared with the traditionalfiltering method.

Amended influence matrix method for removal of rigid motion in the interior BVP for plane elasticity

A conventional complex variable boundary integral equation （CVBIE） in plane elasticity is provided. After using the Somigliana identity between a particular fundamental stress field and a physical stress field, an additional integral equality is obtained. By adding both sides of this integral equality to both sides of the conventional CVBIE, the amended boundary integral equation （BIE） is obtained. The method based on the discretization of the amended BIE is called the amended influence matrix method. With this method, for the Neumann boundary value problem （BVP） of an interior region, a unique solution for the displacement can be obtained. Several numerical examples are provided to prove the efficiency of the suggested method.

Effect of Amended Organic Media and Different Concentrations of Seaweed Extract on the Growth and Flowering of Periwinkle （Vinca rosea） Plant

A pot research has been conducted in Horticultural Department Nursery, Faculty of Agricultural and Forestry, Duhok University, during the period of May 15, 2012-Sep. 1, 2012. The study involved in testing the effects of two amended organic media （loam ＋ sheep manure and loam ＋ peat moss （1：1）） and different concentrations of seaweed （sea force 2） extract including （0, 0.5, 1, 1.5 and 2 mL/L） on growth and flowering of Periwinkle （Vinca rosea） plant. The results showed that growing vinca plants in peat moss medium and spraying sea force 2 extract with low concentrations especially 0.5 mL/L resulted in positive significant difference in vegetative and flowering characteristics.

From Power to Rights: Interpreting Inscribing “To Respect and Protect Human Rights” in the Amended Criminal Procedure Law

Human rights are one of the widely acknowledged value systems in the international ,community. The core of human rights lies in the life and dignity of human beings. On the one hand, criminal procedure law is related to life and freedom, which is the concern of everybody. On the other hand, it involves direct dialogue between national power and citizens＇ fights.

BY-LAWS ON THE CAS MEMBERSHIP (Adopted by the Sixth General Assembly of the Chinese Academy of Sciences in 1992, and Amended by the Seventh General Assembly of the Chinese Academy of Sciences in 1994)

Article 1. The Membership of the Chinese Academy of Sciences (hereinafter referred to as the Member) is the highest academic title of life tenure and honor established by the State in the field of science and technology.

Breakdown of Azadirachtin A in a Tropical Soil Amended with Neem Leaves and Animal Manures

A field investigation was conducted to assess the breakdown of azadirachtin A in a tropical coastal savanna soil amended with neem leaves （NL） combined with poultry manure （PM） or cow dung （CD） using gas chromatography. Samples in polythene bags 15 cm long and 4.8 cm in diameter were randomly placed to a depth of 14 cm in the soil, and azadirachtin A concentration was assessed on days 0, 14, 28, 42, 56, 70, and 84. Azadirachtin A degradation in the soil followed first-order reaction kinetics with different half-lives obtained for varying combinations of the amendments. Higher neem amendment levels of 100 g gave shorter half-lives of azadirachtin A than the lower levels of 50 g. Within the 50 g NL group the additions of the poultry manure and the cow dung gave significantly shorter （P 〈0.05） half-lives of azadirachtin A than the sole neem amendment, whereas in the 100 g NL group only additions of 10 g CD and 10 g PM were significantly less （P 〈 0.05） than the sole neem amendment. Different changes resulting from the kind and quantity of animal manure added were observed in the half-lives of azadirachtin A. The 100 g NL group had significantly higher （P 〈0.05） moisture content, which, coupled with the likely differeaces in microbial biomass, could be the major factor responsible for variations in the half-llfe of the compound. Therefore, the quantity of the neem leaves applied and the addition of animal manure affected the breakdown of azadirachtin A in the soil amended with neem leaves.

Astronomical Algorithms: Amended Multi-Millennia Calendar

Three new worldwide calendars are proposed and compared in this paper. None of them requires any departure from an existing tradition to divide years on lean and leap. Although all three are pretty accurate, it is demonstrated that the Julian calendar with one additional amendment is the simplest and the most suitable for implementation.

Evaluation of Infiltration Capacity and Water Retention Potential of Amended Soil Using Bamboo Charcoal and Humus for Urban Flood Prevention

In Japan, floods occur frequently in urban areas because non-infiltrating areas are seeing increased urbanization. To prevent floods, urban basins must improve the infiltration capacity and water retention of the whole basin. There are several basic technologies for river basin management, such as infiltration trenches or rainwater storage. However, a method of soil amendment that prevents flood disasters has not been established. This study aims to evaluate the infiltration capacity of soil amendments using bamboo charcoal and humus. A constant-head infiltration test and rainfall simulation were conducted to evaluate the properties of the soil amendments. The constant-head infiltration test＇s results showed that soils mixed with 30% humus had the greatest potential for influencing initial and final infiltration rates, and the more the mixing rates of bamboo charcoal and humus were increased, the higher the water retention capacity. The results of the rainfall simulation showed that soils mixed with 30% humus had the highest final infiltration rates and lowest multiplication spillage. To reduce the runoff volume using soil amendment technology, it is important to delay overland flow, and the hydraulic properties of the soils mixed with bamboo charcoal and humus were as effective as those of granite soils.

Recovery of Soil Test Phosphorus from an Acidic Soil Amended with Organic and Inorganic Phosphorus

Information on soil test phosphorus (P) in soil treated with organic amendments is important to a sound management of manure additions to agricultural fields. This study compared the recovery of cow manure, chicken manure, city compost P relative to triple super phosphate P (TSP) for an acidic soil with different antecedent soil test P (STP). Phosphorus was added at rates of 0, 100, 200, 400 and 800 mg P kg-1 soil based on total P. The soil was incubated at field capacity for 1, 4, 8 and 16 weeks (wk) after which they were extracted using NaHCO3 (Olsen) Mehlich-3, Kelowna and Bray & Kurtz-1 extractants. Regardless of extractants, after 1 wk incubation, the highest STP source was the TSP and the least was the city compost. Soil Test P increased with the addition of amendments from different P sources. Among the amendments, soil test P in TSP amended soil gradually decreased but in the city compost amended soil slightly in- creased with incubation time, whereas the changes of soil test P with time in the cow and chicken manures amended soil was very negligible. Across the amendments and rates of P additions, the value of extractable P with Olsen was of 55 mg ?kg-1 (16%), with Mehlich-3 was of 112 mg ?kg-1 (32%), with Kelowna was of 88 mg ?kg-1 (24%) and with Bray & Kurtz was of 104 mg ?kg-1 (29% of total added P). The P extraction efficiency was in the order: NaHCO3 Kelowna < Bray & Kurtz-1 < Mehlich-3. This study indicates that P in organic amendments reflects plant available P through the entire incubation period but P in the TSP are likely to under estimate after 8 wk of incubation.

Heavy Metal Accumulation in Maize (<i>Zea mays</i>L.) Grown on Chromated Copper Arsenate (CCA) Contaminated Soil Amended with Treated Composted Sewage Biosolid

A pot experiment was conducted to investigate the heavy metal accumulation in maize (Zea mays L.) plant grown in chromated copper arsenate (CCA) soil amended with treated composted sewage biosolid. The initial concentrations of chromium, copper, arsenate in the CCA soil and sewage biosolid were determined by atomic absorption spectrophotometer. These were found to be, in CCA soil: 365.8 ± 6.18, 109.22 ± 14.04, 28.22 ± 3.8 and in sewage biosolid: 35 ± 1.06, 1.0 ± 0.02, 0 mg·kg-1 respectively. The concentration of Cr, Cu and As determined in both the roots and shoots generally decreased with increase in percentage amendment concentration and number of days (20 and 40 days after planting). At 20 days, the total metal concentration ranges in roots were As (5.54 ± 0.03 - 6.69 ± 1.14), Cr (9.59 ± 0.02 - 13.22 ± 0.03), Cu (2.28 ± 0.06 - 4.53 ± 0.37) mg·kg-1 while at 40 days the values were As (5.60 ± 0.19 - 6.08 ± 0.01), Cr (9.47 ± 0.04 - 10.95 ± 0.09), Cu (3.94 ± 0.19 - 4.64 ± 0.07) mg·kg-1. For the shoot system, the concentrations of the metals at 20 days were As (5.28 ± 0.03 - 5.90 ± 0.13), Cr (9.30 ± 0.05 - 10.07 ± 0.06), Cu (3.64 ± 0.12 - 4.72 ± 0.15) mg/kg while at 40 days the values obtained were As (5.28 ± 0.03 - 5.9 ± 0.13), Cr (9.69 ± 0.14 - 10.07 ± 0.03), Cu (2.94 ± 0.72 - 4.53 ± 0.03) mg·kg-1. The roots accumulated the three heavy metals more than the shoot system at all treatments used. Concentration of arsenic, chromium and copper in the plants decreased with increasing percentage amendments. The results suggest relatively low bioavailability of the three metals in CCA soil treated with high percentages of sewage biosolid as an amendment.

Theoretical investigations on lattice Boltzmann method:an amended MBD and improved LBM

This paper presents theoretical investigations of lattice Boltzmann method(LBM)to develop a completed LBM theory.Based on H-theorem with Lagrangian multiplier method,an amended theoretical equilibrium distribution function(EDF)is derived,which modifies the current Maxwell–Boltzmann distribution(MBD)to include the total internal energy as its parameter.This modification allows the three conservation laws derived directly from lattice Boltzmann equation(LBE)without additional small-parameter expansions adopted in references.From this amended theoretical EDF,an improved LBM is developed,in which the total internal energy like the mass density and mean velocity is a new macroscopic variable to be updated for different times and cells during simulations.The developed method provides a means to consider external forces and energy generation sources as generalised forces in LBM simulations.The corresponding model and implementation process of the improved LBM are presented with its performance theoretically investigated.Analytically hand-workable examples are given to illustrate its applications and to confirm its validity.The paper will excite more researchers and scientists of this area to numerically practice the new theory and method dealing with complex physical problems,from which it is expected to further advance LBM benefiting science and engineering.

Biochar,Properties and Skills with a Focus on Implications for Vineyard Land and Grapevine Performance

Biochar has emerged as a promising tool for enhancing vineyard sustainability by improving soil properties and mitigating climate change impacts.This review highlights key findings on biochar’s role in viticulture,focusing on its effects on soil fertility,water retention,and plant physiology.Field and pot studies demonstrate that biochar amendments enhance soil structure,increase cation exchange capacity(CEC),and promote water availability,leading to improved drought resistance in grapevines.However,the impacts on grape yield,physiology,and quality remain inconclusive,with some studies reporting benefits while others show neutral effects.Future research should focus on optimizing biochar application rates,understanding its interactions with soil microbiota,and assessing long-term impacts on grape production and wine quality.Additionally,addressing potential risks,such as heavy metal contamination and changes in microbial communities,is crucial for its safe and effective use.This review aims to supply a comprehensive assessment of our knowledge about the incidence and consequences of biochar on soil,including its potential use in soil remediation and concerns regarding its possible negative impacts,with a focus on its effects on vine physiology and grape production.

Long-term combined application of organic and inorganic fertilizers increases crop yield sustainability by improving soil fertility in maize-wheat cropping systems

Organic material combined with inorganic fertilizer has been shown to greatly improve crop yield and maintain soil fertility globally. However, it remains unclear if crop yield and soil fertility can be sustained in the long term under the combined application of organic and inorganic fertilizers. Three long-term field trials were conducted to investigate the effects of organic amendments on the grain sustainable yield index(SYI), soil fertility index(SFI)and nutrient balance in maize–wheat cropping systems of central and southern China during 1991–2019. Five treatments were included in the trials: 1) no fertilization(control);2) balanced mineral fertilization(NPK);3) NPK plus manure(NPKM);4) high dose of NPK plus manure(1.5NPKM);and 5) NPK plus crop straw(NPKS). Over time, the grain yields of wheat and maize showed an increasing trend in all four fertilization treatments at the Yangling(YL) and Zhengzhou(ZZ) locations, while they declined at Qiyang(QY). The grain yield in the NPKM and 1.5NPKM treatments gradually exceeded that of the NPK and NPKS treatments at the QY site. The largest SYI was recorded in the NPKM treatment across the three sites, suggesting that inorganic fertilizer combined with manure can effectively improve crop yield sustainability. Higher SYI values were recorded at the YL and ZZ sites than at the QY site, possibly because the soil was more acid at QY. The key factors affecting grain yield were soil available phosphorus(AP) and available potassium(AK) at the YL and ZZ sites, and pH and AP at the QY site.All fertilization treatments resulted in soil N and P surpluses at the three sites, but soil K surpluses were recorded only at the QY site. The SFI was greater in the 1.5NPKM, NPKM and NPKS treatments than in the NPK treatment by 13.3–40.0 and 16.4–63.6% at the YL and ZZ sites, respectively, and was significantly higher in the NPKM and 1.5NPKM treatments than in the NPK and NPKS treatments at the QY site. A significant, positive linear relationship was found between SFI and crop yield, and SYI and nutrient balance, indicating that grain yield and its sustainability significantly increased with increasing soil fertility. The apparent N, P and K balances positively affected SFI.This study suggests that the appropriate amount of manure mixed with mineral NPK fertilizer is beneficial to the development of sustainable agriculture, which effectively increases the crop yield and yield sustainability by improving soil fertility.

Carbon mineralization in subtropical alluvial arable soils amended with sugarcane bagasse and rice husk biochars

Subtropical recent alluvial soils are low in organic carbon(C).Thus,increasing organic C is a major challenge to sustain soil fertility.Biochar amendment could be an option as biochar is a C-rich pyrolyzed material,which is slowly decomposed in soil.We investigated C mineralization(CO_(2)-C evolution)in two types of soils(recent and old alluvial soils)amended with two feedstocks(sugarcane bagasse and rice husk)(1%,weight/weight),as well as their biochars and aged biochars under a controlled environment(25±2℃)over 85 d.For the recent alluvial soil(charland soil),the highest absolute cumulative CO_(2)-C evolution was observed in the sugarcane bagasse treatment(1140 mg CO_(2)-C kg^(-1)soil)followed by the rice husk treatment(1090 mg CO_(2)-C kg^(-1)soil);the lowest amount(150 mg CO_(2)-C kg^(-1)soil)was observed in the aged rice husk biochar treatment.Similarly,for the old alluvial soil(farmland soil),the highest absolute cumulative CO_(2)-C evolution(1290 mg CO_(2)-C kg^(-1)soil)was observed in the sugarcane bagasse treatment and then in the rice husk treatment(1270 mg CO_(2)-C kg^(-1)soil);the lowest amount(200 mg CO_(2)-C kg^(-1)soil)was in the aged rice husk biochar treatment.Aged sugarcane bagasse and rice husk biochar treatments reduced absolute cumulative CO_(2)-C evolution by 10%and 36%,respectively,compared with unamended recent alluvial soil,and by 10%and 18%,respectively,compared with unamended old alluvial soil.Both absolute and normalized C mineralization were similar between the sugarcane bagasse and rice husk treatments,between the biochar treatments,and between the aged biochar treatments.In both soils,the feedstock treatments resulted in the highest cumulative CO_(2)-C evolution,followed by the biochar treatments and then the aged biochar treatments.The absolute and normalized CO_(2)-C evolution and the mineralization rate constant of the stable C pool(K_(s))were lower in the recent alluvial soil compared with those in the old alluvial soil.The biochars and aged biochars had a negative priming effect in both soils,but the effect was more prominent in the recent alluvial soil.These results would have good implications for improving organic matter content in organic C-poor alluvial soils.

Minimization of methabenzthiazuron residues in leaching water using amended soils and photocatalytic treatment with TiO_2 and ZnO

In the present work, potential groundwater pollution by methabenzthiazuron （MTBU） and the effect of three different amendments （composted sheep manure, composted pine bark and spent coffee grounds） on its mobility were investigated under laboratory conditions. The efficiency of ZnO and TiO2 suspensions in the photocatalytic degradation of MTBU in leaching water was also investigated. The relative and cumulative breakthrough curves were obtained from disturbed soil columns. The presence and/or addition of organic matter drastically reduced the movement of the herbicide. On other hand, photocatalytic experiments showed that the addition of ZnO and TiO2 strongly enhances the degradation rate of this herbicide compared with the results of photolytic experiments under artificial light. ZnO appeared to be more effective in MTBU oxidation than TiO2. The results obtained point to the interest of using organic wastes and heterogeneous photocatalysis for reducing the pollution of groundwater by pesticide drainage.

Volatile organic compound emissions from straw-amended agricultural soils and their relations to bacterial communities:A laboratory study

A laboratory study was conducted to investigate volatile organic compound（VOC） emissions from agricultural soil amended with wheat straw and their associations with bacterial communities for a period of 66 days under non-flooded and flooded conditions. The results indicated that ethene, propene, ethanol, i-propanol, 2-butanol, acetaldehyde, acetone,2-butanone, 2-pentanone and acetophenone were the 10 most abundant VOCs, making up over 90% of the total VOCs released under the two water conditions. The mean emission of total VOCs from the amended soils under the non-flooded condition（5924 ng C/（kg·hr）） was significantly higher than that under the flooded condition（2211 ng C/（kg·hr））. One ＂peak emission window＂ appeared at days 0–44 or 4–44, and over 95% of the VOC emissions occurred during the first month under the two water conditions. Bacterial community analysis using denaturing gradient gel electrophoresis（DGGE） showed that a relative increase of Actinobacteria, Bacteroidetes, Firmicutes and γ-Proteobacteria but a relative decrease of Acidobacteria with time were observed after straw amendments under the two water conditions. Cluster analysis revealed that the soil bacterial communities changed greatly with incubation time, which was in line with the variation of the VOC emissions over the experimental period. Most of the above top 10 VOCs correlated positively with the predominant bacterial species of Bacteroidetes, Firmicutes and Verrucomicrobia but correlated negatively with the dominant bacterial species of Actinobacteria under the two water conditions. These results suggested that bacterial communities might play an important role in VOC emissions from straw-amended agricultural soils.

Excessive manure application stimulates nitrogen cycling but only weakly promotes crop yields in an acidic Ultisol:Results from a 20-year field experiment

Population growth and growing demand for livestock products produce large amounts of manure,which can be harnessed to maintain soil sustainability and crop productivity.However,the impacts of excessive manure application on crop yields,nitrogen(N)-cycling processes and microorganisms remain unknown.Here,we explored the effects of 20-year of excessive rates(18 and 27 Mg ha^(–1)yr^(–1))of pig manure application on peanut crop yields,soil nutrient contents,N-cycling processes and the abundance of N-cycling microorganisms in an acidic Ultisol in summer and winter,compared with none and a regular rate(9 Mg ha^(–1)yr^(–1))of pig manure application.Long-term excessive pig manure application,especially at the high-rate,significantly increased soil nutrient contents,the abundance of N-cycling functional genes,potential nitrification and denitrification activity,while it had a weaker effect on peanut yield and plant biomass.Compared with manure application,seasonality had a much weaker effect on N-cycling gene abundance.Random forest analysis showed that available phosphorus(AP)content was the primary predictor for N-cycling gene abundance,with significant and positive associations with all tested N-cycling genes.Our study clearly illustrated that excessive manure application would increase N-cycling gene abundance and potential N loss with relatively weak promotion of crop yields,providing significant implications for sustainable agriculture in the acidic Ultisols.

Prospects and limitations of soil amendment and irrigation techniques for the water-saving public urban greenery and ephemeral weed management in the sandy soils of the United Arab Emirates

Public urban greenery greatly contributes to the residential and tourist value of cities in the Gulf Region,but due to the hyper-arid climatic conditions,the cost of irrigation and plant maintenance is very high.Existing strategies to reduce the monetary and ecological costs involve the cultivation of native xerophytic plantations,and/or the use of soil improvers to increase water-and nutrient-holding capacity of the sandy soils.Various soil improvers based on mineral,organic,or synthetic materials have entered the United Arab Emirates(UAE)market in recent years,but there is considerable uncertainty about how they should best be used in combination with ornamental plant stands involving xerophytic native plants.The present study investigated the effect of soil amendment and deep pipe irrigation on perennial ornamental plant stands involving native plants(Tephrosia appolinea(Gel.)Link in combination with Aerva javanica(Burm.f.)Juss.ex Schult.)and native-exotic plants(T.appolinea in combination with Ruelia simplex C.Wright)either or not topsoil and subsoil amendment with bentonite and hydrophobic sand under the irrigation water supply of less than 50%of reference evapotranspiration(ET0).After one year of cultivation,T.appolinea and A.javanica(native vs.native)produced high biomass and exhibited high water use efficiency(WUE)as compared with T.appolinea and R.simplex(native vs.exotic)combination given that no significant differences were found under the soil amendment treatments.All stands thrived under irrigation water supply far below what is usually supplied to exotic ornamental stands in public parks of the Al Ain City,the UAE.However,subsoil amendment in combination with deep pipe irrigation reduced the occurrence of weeds and increased the overall plant rooting depth.Our results suggest that subsoil amendment and irrigation up to 60-80 cm depth can potentially control ephemeral weed infestation,which is a great challenge in various plant production systems of the Gulf Region.The results of the present study suggest that the impact of soil amendment on the WUE of exotic plants is marginal and might not be economically justified.Replacing exotic with native ornamental plant species seems to have a far greater water-saving potential than the amendment of the soil,while weeds can be suppressed in the absence of topsoil moisture.

Mineralization and humification of chicken manure and composted kitchen waste in soils based on an in situ litter-bag experiment: impacts of organic inputs and microbial community

Organic inputs are key to increasing soil organic carbon in agricultural soils.This study aimed to unravel the process of mineralization and humification of chicken manure(CM)and composted kitchen waste(KW)using an in situ litter-bag incubation experiment.The results indicated that over 50%,64%to 72%,and 62%to 85%of the initial mass,carbon and nitrogen,respectively,were lost through incubation with a marked loss occurring during the first 28 days.Increased humic acids(HAs),humus(Hs)and degree of humification,along with a decrease in the level of fulvic acids and precursors for humic substances were observed through incubation.By comparison,CM demonstrated higher carbon and nitrogen conservation efficiencies and greater humification compared to KW.Additionally,a higher degree of humifaction and larger quantities of HAs and HS were not favorable for carbon and nitrogen conservation.Further structural equation modeling indicated that microbial community had a strong effect on carbon loss and nitrogen release,while stoichiometric properties of organic inputs were the main determinant of the mineralization and humification processes.These findings will enhance understanding of litter decomposition in soils and provide valuable references for soil carbon sequestration with organic inputs.