Novel wild edible mushroom Astraeus hygrometricus(Pers.)Morgan induces robust apoptosis on human acute lymphoblastic leukemia cells through a RONS-subsisted mitochondria-dependent pathway

Objective:To explore the therapeutic effects of the novel wild edible mushroom Astraeus hygrometricus(Pers.)Morgan(A.hygrometricus)on human acute lymphoblastic leukemia cells.Methods:Extensive screening of the antiproliferative and chemopreventive potential of different extracts from 5 wild mushrooms,A.hygrometricus,Phallus sp.,Lentinus sp.,Tricholoma sp.,and Serpula sp.was performed against a panel of 6 cancer cell lines and normal cells using the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide(MTT)assay.Apoptosis determination,cell cycle profiling,intracellular reactive oxygen species(ROS)and reactive nitrogen species(RNS),and mitochondrial membrane potential were analyzed by flow cytometry.The activity of caspases was measured colorimetrically,and the expression pattern of mitochondrial proteins was analyzed.Results:The methanol extract of A.hygrometricus and MOLT-4 cells were identified as the most potent extract exhibiting antiproliferative activity and most sensitive cell line,respectively.The mushroom extract induced robust selective apoptosis in MOLT-4 cells and arrested cell cycle progression at the G0/G1 stage.The extract disrupted the mitochondrial membrane potential and enhanced ROS production in MOLT-4 cells.The methanol extract induced apoptosis by downregulating the expression of Bcl-2,increasing the expression of Bax,and activating the caspase cascade.Conclusion:The novel wild edible mushroom is a potential repository of biomolecules for the development of antileukemic drugs.

Sexual spores in edible mushroom: bioactive components, discharge mechanisms and effects on fruiting bodies quality

Edible mushroom sexual spores have been gaining more interest due to their bioactive components and functions.Spore discharge(SD)is an important factor affecting the quality of edible mushrooms.In this review,the bioactive nutrients of sexual spores of edible mushrooms were summarized,the SD mechanism was described,and the relationship between postharvest SD and the quality of edible mushrooms was analyzed.Spores contain various bioactive nutrients that are benefi cial to the human body.Mature mushrooms can actively discharge spores in a process affected by light,relative humidity,and temperature.During storage,the physiological metabolism of spore-bearing gill tissue is vigorous,promoting the release of postharvest spores and changing the nutritional value of fruiting bodies.The flavor of the fruiting bodies also varied signifi cantly during SD.Edible mushroom sexual spores have the potential to become new raw materials for functional food and medical resources.Research on the effect of the mechanism of SD on the quality of edible mushrooms and the development of SD regulation technology may be a new trend in the quality control of edible mushrooms,which will promote the development of the edible mushroom industry.

Edible mushrooms as a potent therapeutics of subclinical thyroid dysfunction among adults,especially in obese individuals:a prospective cohort study

Background:Mushrooms are a good source of many nutrients which are potentially beneficial for chronic diseases.We speculated that due to its abundant nutrients edible mushrooms might have a beneficial effect on the prevention of subclinical thyroid dysfunction(SCTD).Therefore,we designed a large-scale cohort study to examine whether mushrooms consumption is a protective factor for SCTD in adults.Methods:This prospective cohort study investigated 6631 participants(mean age:(45.0±10.2)years;55.1%men).Edible mushrooms consumption was measured at baseline using a validated food frequency questionnaire.SCTD was defined as abnormal serum thyroid-stimulating hormone levels and normal free thyroxine.Cox proportional hazards regression models were used to examine the association of edible mushrooms consumption with incident SCTD.Results:During follow-up period,a total of 262 new cases of SCTD were identified,the incidence rate of subclinical hypothyroidism was 8.9/1000 person-years and subclinical hyperthyroidism was 7.2/1000 person-years.After adjusting potential confounding factors,the multivariable hazard ratios(95%confidence intervals)for subclinical hypothyroidism were 1.00(reference)for almost never,0.53(0.29,0.97)for 1-3 times/week and 0.30(0.10,0.87)for≥4 times/week(P for trend=0.02).It also showed edible mushrooms consumption was inversely associated with subclinical hypothyroidism in obese individuals but not non-obese individuals,the final hazard ratios(95%confidence intervals)were 0.14(0.03,0.73)(P for trend<0.01).Conclusions:This population-based prospective cohort study has firstly demonstrated that higher edible mushrooms consumption was significantly associated with lower incidence of subclinical hypothyroidism among general adult population,especially in obese individuals.

A narrative review on inhibitory effects of edible mushrooms against malaria and tuberculosis-the world’s deadliest diseases

The isolated secondary metabolites from 39 edible mushrooms are reported,among which 107 compounds were active,61 demonstrated antitubercular activities with IC_(50) range of 0.2-50μg/mL and 46 manifested antimalarial effects with IC_(50) range of 0.061-36μg/mL.While more than 2000 strains of edible mushrooms are identified,this review shows the paucity of research in these rich organisms featuring a vital culinary ingredient worldwide.A thorough search was conducted on basidiomycetes to discuss the chemistry and biology of the isolated compounds,structure activity relationships(SAR)as well as the cytotoxicity profiles of,primarily,the active anti-plasmodial and antitubercular molecules.With a safe cellular profile,lanostane triterpenoids were found to be the only molecules with combined activities against both diseases.SAR correlations reviewed here indicated the significance of 3β-and 7α-hydroxylation in the anti-tuberculosis activity and the terminal unsaturated moiety between C-4 and C-28 in the antimalarial activity in the same terpene skeleton.This review will attract the attention of medicinal chemists,and food scientists to optimize and rationalize the use of mushrooms both as unexploited sources of novel molecules and as nutraceuticals to treat two of the deadliest infectious diseases,malaria,and tuberculosis.

Minerals in edible insects:a review of content and potential for sustainable sourcing

In response to the rapid increase in world population and subsequent demands for food,edible insects represent an alternative food source for humans that is rich in proteins,amino acids and minerals.Entomophagy is a tradition in many countries including China and Thailand,and edible insects have attracted a lot of attention in Western World due to their suitable nutrient composition,high mineral content(e.g.,Fe,Zn,Ca,Mg)and potential use as a supplement in human diet.In this study,we surveyed mineral content in seven insect orders and 67 species of mass produced and wild-harvested edible insects.The total content of essential elements in edible insects was very high in Tenebrio molitor,Bombyx mori,and Zonocerus variegatus.The heavy metal content(summarized for eight species)was below the maximum limit allowed for safe consumption.Sustainable supply of minerals derived from insect biomass is complicated due to the high variations of mineral content in insects and the potential of its change due to processing.

Matching Dyeing and Properties of Silk Fabrics with Natural Edible Pigments

The silk fabrics were matching dyed with three natural edible pigments(red rice red,ginger yellow and gardenia blue).By investigating the dyeing rates and lifting properties of these pigments,it was observed that their compatibilities were excellent in the dyeing process:dye dosage 2.5%(omf),mordant alum dosage 2.0%(omf),dyeing temperature 80℃and dyeing time 40 min.The silk fabrics dyed with secondary colors exhibited vibrant and vivid color owing to the remarkable lightness and chroma of ginger yellow.However,gardenia blue exhibited multiple absorption peaks in the visible light range,resulting in significantly lower lightness and chroma for the silk fabrics dyed with tertiary colors,thus making it suitable only for matte-colored fabrics with low chroma levels.In addition,the silk fabrics dyed with these three pigments had a color fastness that exceeded grade 3 in resistance to perspiration,soap washing and light exposure,indicating acceptable wearing properties.The dyeing process described in this research exhibited a wide range of potential applications in matching dyeing of protein-based textiles with natural colorants.

Variety Selection and Green Cultivation Technology of Courtyard Ornamental and Edible Peppers

In order to comply with the development trend of the multifunctional use of peppers,we conducted an investigation into the characteristics and features of varieties,potting management techniques,and the methods of extending the fruit ornamental period and other aspects of courtyard ornamental and edible peppers.A set of cultivation techniques suitable for courtyard ornamental and edible peppers has been developed,including timely sowing and seedling,nutrient soil preparation,water and fertilizer management,trimming and pruning,preservation of flowers and fruits,green prevention and control of diseases and pests,harvesting,and so on.

Mannogalactoglucan from mushrooms protects pancreatic islets via restoring UPR and promotes insulin secretion in TIDM mice

Type 1 diabetes mellitus(T1DM) lacks insulin secretion due to autoimmune deficiency of pancreaticβ-cells.Protecting pancreatic islets and enhancing insulin secretion has been therapeutic approaches.Mannogalactoglucan is the main type of polysaccharide from natural mushroom,which has potential medicinal prospects.Nevertheless,the antidiabetic property of mannogalactoglucan in T1DM has not been fully elucidated.In this study,we obtained the neutral fraction of alkali-soluble Armillaria mellea polysaccharide(AAMP-N) with the structure of mannogalactoglucan from the fruiting body of A.mellea and investigated the potential therapeutic value of AAMP-N in T1DM.We demonstrated that AAMP-N lowered blood glucose and improved diabetes symptoms in T1DM mice.AAMP-N activated unfolded protein response(UPR) signaling pathway to maintain ER protein folding homeostasis and promote insulin secretion in vivo.Besides that,AAMP-N promoted insulin synthesis via upregulating the expression of transcription factors,increased Ca^(2+) signals to stimulate intracellular insulin secretory vesicle transport via activating calcium/calmodulin-dependent kinase Ⅱ(CamkⅡ) and cAMP/PKA signals,and enhanced insulin secretory vesicle fusion with the plasma membrane via vesicle-associated membrane protein 2(VAMP2).Collectively,these studies demonstrated that the therapeutic potential of AAMP-N on pancreatic islets function,indicating that mannogalactoglucan could be natural nutraceutical used for the treatment of T1DM.

Biochemical, functional and antioxidant potential of higher fungi cultivated on agro-industrial residues. Part Ⅱ: Cultures on mixtures of spent mushroom substrates and mushroom cropping by-products

Novel substrates consisted of different fresh agro-industrial residues,their corresponding and commercial spent mushroom substrates(i.e.SMS deriving from laboratory-scale experiments and SMS deriving from industrial-scale experiments by Green Zin S.A.-SMS GZ)and Pleurotus waste(PW;stipes/mishappen mushrooms)were used in blends for a new cultivation cycle of Pleurotus ostreatus and P.eryngii mushrooms in bags.Their impact on the biochemical properties(intra-cellular polysaccharides-IPSs,protein,lipid,total phenolic compounds-TPCs,individual carbohydrates composition of the IPSs)in the first-and second-flush whole mushrooms,pilei and stipes,as well as the fatty acids composition,the antioxidant activity(in the first-flush mushroom parts)and glucan content of stipes were examined.Both species produced satisfactory IPSs quantities in all substrates(28.69-46.38%,w/w)and significant protein amounts(18.37-26.80%,w/w).The further SMS addition(80%,w/w instead of 40%,w/w)in the cultivation substrates affected positively the mushroom IPSs values,whereas the highest protein content was detected in mushroom’s parts cultivated on substrates consisted of fresh agro-industrial residues.Mushroom’s lipid content was affected differently by the various substrate combinations,with SMS presence resulting in mushrooms with a lower fat content than those produced in substrates with PW addition.Fresh substrates with PW and those with coffee residue were the most favorable for TPCs production.Regarding production flushes,the nutritional value of mushrooms was comparable between them,only a slight increase in TPCs of second-flush carposomes was detected.Glucose was the predominant monosaccharide of the produced IPSs,combined with a significant production of total and β-glucans.SMSs and PW addition had a positive impact on antioxidant activity,too.A higher quantity of lipids,TPCs and significant antioxidant activity were detected in all Pleurotus pilei than stipes,whereas the latter were richer in IPSs.Both pilei and stipes had a significant protein amount.Hence,the data obtained by this study support the positive effect of different types of SMS and mushroom waste on P.ostreatus and P.eryngii nutritional value.

Study on Cotton Nursery Substrate Using Cotton Seed Hulls Residue after Producing Edible Mushroom

In order to discuss the localization application technology of cotton seedling using substrate, the author used cotton seed hulls after the production of edible fungi residue, river sand and peat in different proportion formula using in field and laboratory cotton seedling test. The results showed that： dry mass per plant and leaf area per plant of formula 3 were higher than Jiangxi cotton seedling nurs- ery substrate.the higher proportion were 48.4% and 73.5%; the rate of forming plantlets, survival rate of transplant and the unginned cotton yield had no obvious difference with the other matrix seedling and transplanting; nursery substrate was returned using as fertilizer, reduced environment pollution, achieved comprehensive utilization and cyclic utilization. Cotton seed hulls after the production of edible fungi residue was a cotton seedling substrate material of saving work,reducing cost and protecting environment and would have a good application prospect.

Mini-review on edible mushrooms as source of dietary fiber:Preparation and health benefits

Dietary fiber and high-fiber food products have attracted great attention because of their significant health benefits to consumers.Mushrooms are valuable resources for food,medicine and nutraceuticals.Edible mushroom is considered as a novel source of dietary fiber.The dietary fiber content and composition in edible mushroom vary greatly with its morphological stages including fruit body,mycelium and sclerotium.The focus of this mini-review is on the preparation of dietary fiber from edible mushroom with emphasis on the sclerotium which has the highest level of non-starch polysaccharides.The possible health benefits of mushroom dietary fiber in relationship with boosting the immune system,anticancer functions as well as the control of blood lipids and glucose levels are also discussed.©2013 Beijing Academy of Food Sciences.Production and hosting by Elsevier B.V.All rights reserved.

Physicochemical Characterization of Two Species of Wild Edible Mushrooms: <i>Lentinus brunneofloccosus pegler</i>and <i>Auricularia auricularia judae</i>

In tropical Africa, mushrooms play an important role for people in rural areas as a source of food, medicine and income. In order to promote the mycology sector in Côte d’Ivoire, a study was carried out on the determination of the biochemical parameters of the mushroom Lentinus brunneofloccosus pegler and Auricularia auricularia judae found in the localities of Daloa and Yamoussoukro. The samples were purchased in the markets of Daloa and Yamoussoukro. The dry matter, ash and lipid contents were determined by the AOAC method. Those of proteins, total sugars, reducing sugars were carried out respectively by the methods of Kjeldahl, Bernfeld and Dubois, and six minerals (potassium, phosphorus, calcium, magnesium, iron and sodium) were quantified by atomic absorption spectrophotometry (AAS). The results showed that the ash (3.46 - 7.74%) and protein (6.10 - 12.40%) levels were high, but the lipid contents (4% to 6.60%), total sugars (1.34 - 5.16%) and reducing sugars (0.01 to 0.27%) are low. The major minerals are calcium (898 to 1006 mg/100g), potassium (1182 to 2433 mg/100g) and phosphorus (2091 - 4375 mg/100g). Magnesium (270 - 433 mg/100g) is represented in average proportion. Iron (2.83 to 46.17 mg/100g) and sodium (4.87 to 5.99 mg/100g) are in low amounts. The mushrooms Lentinus brunneofloccosus pegler and Auricularia auricularia judae are low in fat, high in ash, and are good sources of protein and minerals.

Quantification of Heavy Metal Accumulation in Edible Wild-Mushrooms in Copperbelt and Western Provinces of Zambia

The mushrooms are highly regarded as one of the most nutritious foods across the globe but also recognized bio-accumulators of heavy metals. The nature and level of industrial activities are continually changing and affecting the environment adversely. The mushrooms are not an exception and may inevitably have heavy metal contaminations. In this vein, this study aimed to determine heavy metal (Cu, Ni, Co, Zn, Pb, and Cd) uptake levels in wild edible mushrooms from the sites with different economic activities. The wild mushrooms considered for this study included Tente (Amanita Zambiana), Ichikolowa (Termitomyces Titaniscus), and Kabansa (Lactarius Tataniscus). The analysis of heavy metal concentration was carried out using atomic absorption spectrometry (AAS). For the selected mushrooms, concentration ranges (mg&middot;kg-1 total dry weight (dw)) of 46.90 - 141.80 for Cu, 0.10 - 6.60 for Cd, 1.10 - 2.00 for Pb, 19.00 - 38.90 for Zn, 1.00 - 3.40 for Ni, and 44.80 - 79.70 for Co were obtained. However, for the respective soil samples, concentration ranges (mg&middot;kg-1&middot;dw) of 51.00 - 279.40 for Cu, 1.00 - 99.50 for Cd, 8.00 - 10.00 for Pb, 22.80 - 209.10 for Zn, 9.00 - 33.70 for Ni, and 60.00 - 111.90 for Co were obtained. To a certain degree, the concentrations reflected the impact of diversity in the surrounding activities. This study discovered that for the selected mushrooms, the contamination level of cadmium, cobalt, nickel, and copper exceeded the World Health Organization (WHO)/FAO (Food and Agriculture Organization) recommended limits. Although some minor aberrations from the prescribed limits were also observed in the case of copper and nickel. Further, the data established that the heavy metal concentrations in respective soils are not the sole determinant of concentrations in mushrooms. Thus, these findings merit attention as, in some cases, the extent of contamination has exceeded the WHO permissible limit, and it may pose a health risk to consumers.

Accumulation of Natural Radionuclides by Some Edible Wild Mushrooms in Ekiti State, Southwestern, Nigeria

Activity concentrations of primordial radionuclides such as 40K, 226Ra and 232Th were determined in edible mushrooms samples in Ekiti State Nigeria. The edible mushrooms collected are Termitomyces striatus, Psathyrella atroumbonata, Termitomyces robustus, Pleurotus tuber-regium and Pleurotus squarrosulus. The activity measurements were carried out by gamma spectrometry. The average concentrations of 40K varied from 254.17 ± 46.78 to 416.07 ± 68.43 Bq&middot;kg-1, 226Ra concentrations varied from 2.68 ± 0.82 to 21.64 ± 7.23 Bq&middot;kg-1 and 232Th concentrations varied from 8.57 ± 3.25 to 10.98 ± 4.31 Bq&middot;kg-1. The concentrations were converted to effective dose. Effective doses calculated were found to be below maximum permissible levels. Therefore, no health risk is envisaged for those that normally consumed these mushrooms.

Comparison of Protein and Amino Acids in the Extracts of Two Edible Mushroom, <i>Pleurotus sajor-caju</i>and <i>Schizophyllum commune</i>

This study was undertaken to determine total protein (%) and profiles of amino acid and made comparison between the aqueous and organic solvent extracted mushroom. Extraction was made from two edible, Pleurotus sajor-caju (commercial) and Schizophyllum commune (wild) types of mushrooms. Four types of solvents were used for the extraction include 100% aqueous, 50% ethanol, 50% methanol and 50% acetone. True protein of mushroom extract was analyzed with colorimetric Lowry method and amino acids were determined by using high-performance liquid chromatograph (HPLC). The range of 1.06% to 3.43% and 1.30% to 2.17% total protein value were obtained in the extracts of P. sajor-caju and S. commune respectively, while the highest total protein of 3.43% was determined in aqueous extracted P. sajor-caju mushroom. The amount of total amino acids of S. commune and P. sajor-caju were in the range of 308.65 mg/g to 443.84 mg/g and 172.52 mg/g to 400.76 mg/g, respectively. The highest content of 443.84 mg/g total amino acids and 77.08 mg/g of essential amino acids were obtained in the aqueous extracted Schizophyllum commune. On the other hand the total content of essential amino acids (EAA). Essential amino acid of both mushrooms was dominated by leucine along with threonine and alanine, but the highest contents were determined from the extract of Schizophyllum commune. Aqueous extraction was effective in both types of mushroom for the protein components as well essential amino acids compared to other organic solvents that were used in extraction process in this study.

Preliminary Study on the Ecological Agriculture Development Model of"Pennisetum sineseRoxb-Rocky Desertification Control-Edible Mushrooms"in Guizhou Province

In Guizhou Province, the area of rocky desertification land is 3.023 8 million hm^2, accounting for 17.16% of total area of land in the province. Rocky desertification is the most important ecological problem that restricts the economic and social development in Guizhou, so the primary task of ecological construction in Guizhou Province is to curb rocky desertification. How to effectively, efficiently and persistently use rocky desertification land is not only the key to the improvement of ecological environment, but also an urgent problem solved for local people's survival and anti-poverty. Due to having developed roots, growing fast, high yield of grass, strong resistance to drought, and high regeneration capacity, Pennisetum sinese Roxb has become a new method to control rocky desertification. How to use P. sinese Roxb efficiently becomes the key to the ecological control of rocky desertification by P. sinese Roxb. In this study, the ecological agriculture development model of "P. sinese Roxb-rocky desertification control-edible mushrooms" was studied, and the effect of cultivating Pleurotus eryngii with P. sinese Roxb in rocky desertification land was analyzed. Moreover, an outlook for the application of P. sinese Roxb in rocky desertification areas of Guizhou was given.

Assessment of the Diversity, Distribution and Uses of Wild Edible Mushrooms in Ekiti State, Nigeria

Lack of information about the available species of mushroom, the distribution along different ecological zones and purposes for which they have been used has hampered their domestication for sustainable production. This study assessed the diversity, distribution and uses of wild edible mushrooms in Ekiti State. The study area was stratified based on vegetation distribution into two zones （rainforest and derived savanna zones）. Pre-tested semi-structured questionnaires were administered to 80 randomly selected respondents for data collection. Descriptive statistical technique of frequency counts and percentages were used to summarize the data. Information from the study revealed that 52.5% and 67.5% of the respondents are above 50 years in the rain forest and the derived savannah vegetation, respectively. About 57.5% and 47.5% of the respondents had at least secondary education in the rainforest and derived savannah vegetation, respectively, while majority of the respondents with over 20 years of collection experience carried out their hunting in free areas outside forest reserve in both ecological zones. Twenty different species of mushroom are collected at different period of the year for consumption. The study recommended that government should checkmate further destruction of the remaining natural forest to ensure continued availability of these mushrooms.

An Experimental Study of Vermicomposting with Earthworm （Eisenia foetida） Growth in Edible Mushrooms Wastes

Every year mushroom wastes are produced in big volumes from edible mushroom production industries. Vermicomposting technology using earthworms as versatile natural bioreactors for effective recycling of organic wastes to the soil is an environmentally acceptable means of converting waste into nutritious composts for crop production. This study was undertaken to use earthworms （eisenia foetida） in vermicomposting on edible mushrooms wastes. The Randomized block design with three treatments in Kimia, Sabalan and Artashahr mushroom production industries were selected and four replications were applied for treatments. The total biomass of earthworms was estimated by counting the number of adults, juveniles and cocoons from each replication. The number of adult and mature worms, egg capsules and wet weight of earthworms were recorded in one month intervals （totally in five months）. Vermicomposting was carried out by these worms in four months in spite of delay in their establishment in mushroom wastes. The worms wet weight, their number and the number of capsules were increased up to fourth month and then decreased. The produced vermicompost had suitable properties for amending soil.

Three Major Effects and Guarantee System for Quality Management of Edible Mushroom Products

In order to provide consumers with more safe and edible fungus products and to create a faithful social environment of food quality, the edible fungus products quality safety events, as well as their evoked factors and effects on edible fungus industry were discussed in depth in this paper. The performance of edible fungus products quality management is a comprehensive reflection of technical effect, control effect and implementation effect. Among them, the implementation effect is the most important, which refers to the actual implementation effect of quality and safety rules by practitioners of edible fungus industry. Thus, the initiative implementation effect in quality management will be improved, and a quality assurance system in which practitioners are volunteered to participate and implement initiatively the quality standards and regulations so as to provide safe and high-quality products will be established, thereby achieving the best actual implementation effect in quality management.

Changes of structure and functional properties of rice protein in the fresh edible rice during the seed development

It has been reported that fresh edible rice has more bioactive compounds and its protein is easier to digest and has lower hypoallergenic than mature rice. In this paper, the changes in structure and functional properties of proteins at five different stages, including early milky stage(EMS), middle milky stage(MMS), late milky stage(LMS), waxy ripe stage(WS)and ripening stage(RS), during the seed development were investigated. It was found that with the seed developing, the molecular weight of fresh rice protein gradually become larger while the secondary structure changed from the highest content of disordered structure at MMS to the highest content of ordered structure at RS, which affect the surface hydrophobicity and then the functional properties of proteins, including foaming properties, emulsifying properties and oil holding capacity. Fresh rice protein at MMS has the strongest surface hydrophobicity while fresh edible rice protein at RS has the strongest oil holding capability. The results of our study can provide a theoretical basis for the application of fresh rice protein in the food industry and help to develop new fresh edible rice food.