A pot experiment was conducted to examine the effects of a phosphate solubilizing bacterium(PSB),Enterobacteria sp.EnHy-401,on the availability of insoluble accumulative phosphorus(P)and growth of wheat(Triticum ...A pot experiment was conducted to examine the effects of a phosphate solubilizing bacterium(PSB),Enterobacteria sp.EnHy-401,on the availability of insoluble accumulative phosphorus(P)and growth of wheat(Triticum Ningmai No.13)plants in sterile saline soil.Our results showed that the strain EnHy-401 had the ability to activate the insoluble accumulated phosphorus in saline soil and enhanced nutrient uptake efficiency by wheat plants,then conferred resistance in wheat plants to salt stress and resulted in a significant growth increase.In saline soil inoculated with Enterobacteria sp.EnHy-401,available phosphorus and exchangeable calcium was increased from 6.4 mg/kg and 1 162 mg/kg to 10.3 mg/kg and 1 214 mg/kg,respectively.Wheat seedling grown in soil inoculated with the EnHy-401 strain increased shoot weight by 28.1% and root weight by 14.6% when compared to the control.P,Ca,K and Mg contents in shoots increased 34.4%,36.3%,31.5%,and 6.3% compared to the control,respectively.the fact that the increases in available P,biomass P,and Ca2+ concentration in saline soil treated with PSB Enterobacter sp.EnHy-401 inocula,and high relativity between the P,Ca,K,and Mg content in wheat tissue and dry matter indicated that PSB Enterobacter sp.EnHy-401 suppressed the adverse effect of salinity stress in plants through nutrient(P and Ca)supply and nutrient(K and Mg)uptake enhancement.The phosphate solubilizing activity of Enterobacteria sp.EnHy-401 and the amelioration of salt stress on wheat plants by the strain varied with the salinity levels and content of organic matter in the saline soil.展开更多
Histamine fish poisoning (HFP) is a major illness occurring throughout the world due to the consumption of quality of deteriorated fish containing pre-formed histamine from bacterial activities. In the study reported ...Histamine fish poisoning (HFP) is a major illness occurring throughout the world due to the consumption of quality of deteriorated fish containing pre-formed histamine from bacterial activities. In the study reported here, the histamine-producing bacteria were isolated from the muscle, gills and the gut of 19 samples of Indian mackerel (Rastrelliger kanagurta) from Mumbai, India. The isolates from modified Niven’s medium (MNM) were confirmed for their ability to produce histamine by using 4 different pH-indicator media, followed by HPLC analyses. Out of 202 isolates, 63 isolates produced considerable amounts of histamine on at least 3 out of 4 media used in this study. The histamine formers were identified by biochemical tests followed by sequencing of their 16SrDNA gene, which showed that 89% of the isolates belonged to 13 different genera of the family Enterobacteriaceae. The non-enterobacterial histamine-producing bacteria belonged to the genera Staphylococcus, Alkaligenes, Shewanella and Psychrobacter.展开更多
Objective:To investigate the isolation of enterobacteria associated with Macrobrachium amazonicum(M.amazonicum) farming and evaluate the in vitro antimicrobial susceptibility of Vibrio strains.Methods:Strains were iso...Objective:To investigate the isolation of enterobacteria associated with Macrobrachium amazonicum(M.amazonicum) farming and evaluate the in vitro antimicrobial susceptibility of Vibrio strains.Methods:Strains were isolated from female M.amazonicum prawns and environmental and hatchery water.Biochemical assays were used to identify bacterial genera and those belonging to the genus Vibrio were submitted to further analyses for species identification,through Vitek 2 automated system and scrotyping.Susceptibility test was performed according to Clinical Laboratory Standards Institute.Results:The following genera of enlerobacteria were recovered:Enterobacter(n=11),Citrobacter(n=10),Proteus(n=2),Serratia(n=2),Kluyvera(n=2),Providencia(n=2),Cedecea(n=1),Escherichia(n=1).Edwardsiella(n=1) and BulliauxeUa(n=1).As for Vibrio,three species were identified:Vibrio cholerae non-O1/non-O139(n=4).Vibrio vulnificus(V.vulnificus)(n=1) and Vibrio mimicus(n=1).Vibrio spp.showed minimum inhibitory concentrations values within the susceptibility range established by Clinical Laboratory Standards Institute for almost all antibiotics,except for V.vulnificus,which presented intermediate profile to ampicillin.Conclusions:Enterobacteria do not seem to be the most important pathogens associated with M.amazonicum farming,whereas the recovery of Vibrio spp.from larviculture,with emphasis on Vibrio cholerae and V.vulnificus,deserves special attention due to their role as potentially zoonotic aquaculture-associated pathogens.Furthermore,the intermediate susceptibility of V.vulnificus to ampicillin reflects the importance of monitoring drug use in prawn farming.展开更多
Introduction: Entero bacteria are mainly found in the gut of man and animals. The frequent acquisition of antibiotic resistance mechanisms explains why they are the bacteria most often implicated in human infectious p...Introduction: Entero bacteria are mainly found in the gut of man and animals. The frequent acquisition of antibiotic resistance mechanisms explains why they are the bacteria most often implicated in human infectious pathology. It is estimated to be involved in 50% of sepsis, 60% of enteritis, 70% of urinary tract infection case. Objective: To determine the prevalence of enterobacterial infections diagnosed at Fann Infectious Diseases Clinic, and describe their epidemiological, clinical, therapeutic and evolutionary aspects. Patients and Methods: This is a retrospective and descriptive study, on patients hospitalized from January 2013 to December 2014, at Fann Infectious Diseases Clinic, with bacteriological confirmation of an enterobacteria infection. Results: A total of 129 cases were collected during the study period. The average age was 41 years, and female were predominant (60%) with a sex ratio of 0.67. Comorbidity was found in 88.4% of the cases, most of which were HIV infection. The most common clinical signs were infectious syndrome (53.49%) and general impairment (40.31%). The main gateway was urinary (55.8%). Samples were monomicrobial in 76.7% of cases. Klebsiella and Escherichia were the most common and 68.7% of the subjects had probabilistic treatment. Most enterobacterial strains were resistant to third generation cephalosporins (C3G), aminoglycosides to ciprofloxacin and cotrimoxazole. Aside from 4% of them, all were sensitive to imipenem. Conclusion: The advent of antibiotics has brought hope in the treatment of enterobacterial infections. However, an increase in their resistance to the usual antibiotics has been noted in recent years. As a result, the fight against antibiotic resistance must be a priority.展开更多
The improper use of antimicrobials against infectious diseases has allowed microorganisms to develop defense mechanisms that give them insensitivity to these agents. All bacteria are concerned by this phenomenon. This...The improper use of antimicrobials against infectious diseases has allowed microorganisms to develop defense mechanisms that give them insensitivity to these agents. All bacteria are concerned by this phenomenon. This work aimed to assess prevalence of beta-lactamase produced by enterobacterial isolates. Then, disc diffusion, double disc synergy test (DDST) and combined disc test (CDT) were respectively used for antimicrobial resistance, detection of Extended-Spectrum Beta-Lactamases (ESBL) and Metallo-Beta-Lactamases (MBL). bla genes were detected by PCR. A total of 132 enterobacterial strains were studied. Resistance to antibiotic families was observed with a greater frequency than 50%. Gentamicin was the least active beta-lactam antibiotic, with a resistance rate of 88%. 40.9% of strains show an ESBL phenotype and 16.6% were MBL. An overall prevalence of 74% (40/54) and respectively rates of 29.6%, 27.7% and 16.7% for blaSHV, blaCTX and blaTEM genes were observed. SHV, CTX, CTX/SHV/TEM, CTX/TEM, SHV/TEM and CTX/SHV were different ESBL genotypes observed. ESBL-producing enterobacteria isolation worried about the future of antimicrobial therapy in the Republic of Congo. This is a public health problem that requires careful monitoring and implementation of a policy of rational antibiotics use.展开更多
In silico genome mining provides easy access to secondary metabolite biosynthetic gene clusters(BGCs)encoding the biosynthesis of many bioactive compounds,which are the basis for many important drugs used in human med...In silico genome mining provides easy access to secondary metabolite biosynthetic gene clusters(BGCs)encoding the biosynthesis of many bioactive compounds,which are the basis for many important drugs used in human medicine.However,the association between BGCs and other functions encoded in the genomes of producers have remained elusive.Here,we present a systems biology workflow that integrates genome mining with a detailed pangenome analysis for detecting genes associated with a particular BGC.We analyzed 3,889 enterobacterial genomes and found 13,266 BGCs,represented by 252 distinct BGC families and 347 additional singletons.A pangenome analysis revealed 88 genes putatively associated with a specific BGC coding for the colon cancer-related colibactin that code for diverse metabolic and regulatory functions.The presented workflow opens up the possibility to discover novel secondary metabolites,better understand their physiological roles,and provides a guide to identify and analyze BGC associated gene sets.展开更多
The discovery and application of antibiotics in animal feeds have boomed the development of intensive animal husbandry in the last century,until the emergence of antibiotics-resistant bacteria.To alleviate the risks a...The discovery and application of antibiotics in animal feeds have boomed the development of intensive animal husbandry in the last century,until the emergence of antibiotics-resistant bacteria.To alleviate the risks aroused by antibiotics-resistant bacteria,effective antibiotic substitutes are urgently needed to replace antibiotics.Essential oils(EOs)derived from plants are illustrated as the promising antibiotic substitutes used in animal feeds,as same as their current views for poultry and livestock industries in the future.It has been widely demonstrated that the phytochemicals in EOs show multiple biofunctionability and are less likely to induce resistance in bacteria.The beneficial effects of EOs feed supplementation on the intestinal inflammation,intestinal flora,immunity,digestion,and growth performances have been already extensively examined.However,the cost-effectiveness,odor,volatility,instability and bioavailability are the challenges in effectively utilizing EOs in animal intestines.Based on previous researches,and these challenges can partially be resolved by microencapsulation and nanotechnology are promising techniques to deal with these challenges.This article presents the feasibility and foundation of EOs application as antibiotic substitutes in animal feeds,and illustrates the mechanisms,functional performances and superiority of EOs compared with antibiotics.展开更多
A previous study was conducted on the synthesis and antibacterial evaluation of Mannich bases of 2-(thioalkyl)-1H-methylbenzimidazole derivatives. The results of this study showed that certain 2-(thioalkyl)-1H-methylb...A previous study was conducted on the synthesis and antibacterial evaluation of Mannich bases of 2-(thioalkyl)-1H-methylbenzimidazole derivatives. The results of this study showed that certain 2-(thioalkyl)-1H-methylbenzimidazole and 2-(thioalkyl)-methyl-1-(piperidin-1-ylmethyl)benzimidazole derivatives possess antibacterial activities against clinical strains, such as Escherichia coli, Klebsiella pneumonia (Gram-negative bacteria), Staphylococcus aureus and Pseudomonas aeruginosa (Gram-positive bacteria). Following these favorable results, we extended the antibacterial evaluation of this series of molecules to environmental strains. The aim of this study was to assess the impact of the methyl-piperidine group fixed at position-1 of this new series of benzimidazoles on the antibacterial activity of environmental strains. In addition, we first evaluated the antibacterial activity of four 2-(thioalkyl)methylbenzimidazole derivatives and then that of five 2-(thioalkyl)-methyl-1-(piperidin-1-ylmethyl) benzimidazole derivatives. This study allowed us to show that compounds 1d and 1e could inhibit bacterial growth in vitro of the Enterobacteria P1 strain with inhibition diameters of 17.3 ± 0.6 mm and 10 ± 0.0 mm, respectively. However, addition of methyl-piperidinyl in this series showed that five (5) of 2-(thioalkyl)-methyl-1-(piperidin-1-ylmethyl) benzimidazole derivatives had an inhibitory effect on the in vitro growth of bacterial strains used except on Enterobacteria P2 with inhibition diameters between 10.0 ± 0.8 mm and 27.9 ± 1.4 mm. The introduction of the methyl-piperidinyl group at the 1-position of 2-(thioalkyl)-1H-methylbenzimidazole derivatives greatly improved the antibacterial activity against environmental bacteria such as Escherichia coli A1, A2, A3, and A4 and two Enterobacteria P1.展开更多
Neonatal thrombocytopenia accounts for 20% of neonates hospitalized in the neonatal intensive care unit (NICU) at DPH. The etiologies are multiple, but bacterial infection is the third leading cause of neonatal mortal...Neonatal thrombocytopenia accounts for 20% of neonates hospitalized in the neonatal intensive care unit (NICU) at DPH. The etiologies are multiple, but bacterial infection is the third leading cause of neonatal mortality worldwide. We therefore set out to assess the frequency of neonatal thrombocytopenia associated or not with bacterial infection in the NICU. We conducted a retrospective and prospective study with the DPH NICU, over 10 months (August 2018 and April 2019). Thrombocytopenia encountered in the NICUs, were the subject of research into bacteriological, inflammatory, and epidemiological parameters using Inlog laboratory data processing software. During this period, 1280 babies were hospitalized, 94 of whom underwent thrombocytopenia, corresponding to 7.34%, with a sex ratio of 0.92. The number of babies presenting with thrombocytopenia during the first week of hospitalization was 72, accounting for 76.6%. The clinical context was usually low birth weight in 30.8% of cases and perinatal asphyxia (25%). Thrombocytopenia ranged from 2000 to 137,000 with an average of 69,520/mm3. Among these thrombocytopenias, 64 cases (68%) were below 100,000 mm3 and 44 cases had a CRP >5 mg/l. A total of 30 bacteria were isolated, including 23 Enterobacteria, 2 Streptococci, and 1 Acinetobacter. Among these enterobacteria, 14 were multidrug-resistant (MDR). Thrombocytopenia associated with a multidrug-resistant bacterial infection is a real challenging management.展开更多
基金Supported by Key Technologies R&D Program of Shanghai Municipal Agricultural Commission(X9810)~~
文摘A pot experiment was conducted to examine the effects of a phosphate solubilizing bacterium(PSB),Enterobacteria sp.EnHy-401,on the availability of insoluble accumulative phosphorus(P)and growth of wheat(Triticum Ningmai No.13)plants in sterile saline soil.Our results showed that the strain EnHy-401 had the ability to activate the insoluble accumulated phosphorus in saline soil and enhanced nutrient uptake efficiency by wheat plants,then conferred resistance in wheat plants to salt stress and resulted in a significant growth increase.In saline soil inoculated with Enterobacteria sp.EnHy-401,available phosphorus and exchangeable calcium was increased from 6.4 mg/kg and 1 162 mg/kg to 10.3 mg/kg and 1 214 mg/kg,respectively.Wheat seedling grown in soil inoculated with the EnHy-401 strain increased shoot weight by 28.1% and root weight by 14.6% when compared to the control.P,Ca,K and Mg contents in shoots increased 34.4%,36.3%,31.5%,and 6.3% compared to the control,respectively.the fact that the increases in available P,biomass P,and Ca2+ concentration in saline soil treated with PSB Enterobacter sp.EnHy-401 inocula,and high relativity between the P,Ca,K,and Mg content in wheat tissue and dry matter indicated that PSB Enterobacter sp.EnHy-401 suppressed the adverse effect of salinity stress in plants through nutrient(P and Ca)supply and nutrient(K and Mg)uptake enhancement.The phosphate solubilizing activity of Enterobacteria sp.EnHy-401 and the amelioration of salt stress on wheat plants by the strain varied with the salinity levels and content of organic matter in the saline soil.
文摘Histamine fish poisoning (HFP) is a major illness occurring throughout the world due to the consumption of quality of deteriorated fish containing pre-formed histamine from bacterial activities. In the study reported here, the histamine-producing bacteria were isolated from the muscle, gills and the gut of 19 samples of Indian mackerel (Rastrelliger kanagurta) from Mumbai, India. The isolates from modified Niven’s medium (MNM) were confirmed for their ability to produce histamine by using 4 different pH-indicator media, followed by HPLC analyses. Out of 202 isolates, 63 isolates produced considerable amounts of histamine on at least 3 out of 4 media used in this study. The histamine formers were identified by biochemical tests followed by sequencing of their 16SrDNA gene, which showed that 89% of the isolates belonged to 13 different genera of the family Enterobacteriaceae. The non-enterobacterial histamine-producing bacteria belonged to the genera Staphylococcus, Alkaligenes, Shewanella and Psychrobacter.
基金supported by the National Council for Scientific and Technological Development(445670/2014-2)Coordination Office for the Improvement of Higher Education Personnel(AEI-0052-000650100/11)
文摘Objective:To investigate the isolation of enterobacteria associated with Macrobrachium amazonicum(M.amazonicum) farming and evaluate the in vitro antimicrobial susceptibility of Vibrio strains.Methods:Strains were isolated from female M.amazonicum prawns and environmental and hatchery water.Biochemical assays were used to identify bacterial genera and those belonging to the genus Vibrio were submitted to further analyses for species identification,through Vitek 2 automated system and scrotyping.Susceptibility test was performed according to Clinical Laboratory Standards Institute.Results:The following genera of enlerobacteria were recovered:Enterobacter(n=11),Citrobacter(n=10),Proteus(n=2),Serratia(n=2),Kluyvera(n=2),Providencia(n=2),Cedecea(n=1),Escherichia(n=1).Edwardsiella(n=1) and BulliauxeUa(n=1).As for Vibrio,three species were identified:Vibrio cholerae non-O1/non-O139(n=4).Vibrio vulnificus(V.vulnificus)(n=1) and Vibrio mimicus(n=1).Vibrio spp.showed minimum inhibitory concentrations values within the susceptibility range established by Clinical Laboratory Standards Institute for almost all antibiotics,except for V.vulnificus,which presented intermediate profile to ampicillin.Conclusions:Enterobacteria do not seem to be the most important pathogens associated with M.amazonicum farming,whereas the recovery of Vibrio spp.from larviculture,with emphasis on Vibrio cholerae and V.vulnificus,deserves special attention due to their role as potentially zoonotic aquaculture-associated pathogens.Furthermore,the intermediate susceptibility of V.vulnificus to ampicillin reflects the importance of monitoring drug use in prawn farming.
文摘Introduction: Entero bacteria are mainly found in the gut of man and animals. The frequent acquisition of antibiotic resistance mechanisms explains why they are the bacteria most often implicated in human infectious pathology. It is estimated to be involved in 50% of sepsis, 60% of enteritis, 70% of urinary tract infection case. Objective: To determine the prevalence of enterobacterial infections diagnosed at Fann Infectious Diseases Clinic, and describe their epidemiological, clinical, therapeutic and evolutionary aspects. Patients and Methods: This is a retrospective and descriptive study, on patients hospitalized from January 2013 to December 2014, at Fann Infectious Diseases Clinic, with bacteriological confirmation of an enterobacteria infection. Results: A total of 129 cases were collected during the study period. The average age was 41 years, and female were predominant (60%) with a sex ratio of 0.67. Comorbidity was found in 88.4% of the cases, most of which were HIV infection. The most common clinical signs were infectious syndrome (53.49%) and general impairment (40.31%). The main gateway was urinary (55.8%). Samples were monomicrobial in 76.7% of cases. Klebsiella and Escherichia were the most common and 68.7% of the subjects had probabilistic treatment. Most enterobacterial strains were resistant to third generation cephalosporins (C3G), aminoglycosides to ciprofloxacin and cotrimoxazole. Aside from 4% of them, all were sensitive to imipenem. Conclusion: The advent of antibiotics has brought hope in the treatment of enterobacterial infections. However, an increase in their resistance to the usual antibiotics has been noted in recent years. As a result, the fight against antibiotic resistance must be a priority.
文摘The improper use of antimicrobials against infectious diseases has allowed microorganisms to develop defense mechanisms that give them insensitivity to these agents. All bacteria are concerned by this phenomenon. This work aimed to assess prevalence of beta-lactamase produced by enterobacterial isolates. Then, disc diffusion, double disc synergy test (DDST) and combined disc test (CDT) were respectively used for antimicrobial resistance, detection of Extended-Spectrum Beta-Lactamases (ESBL) and Metallo-Beta-Lactamases (MBL). bla genes were detected by PCR. A total of 132 enterobacterial strains were studied. Resistance to antibiotic families was observed with a greater frequency than 50%. Gentamicin was the least active beta-lactam antibiotic, with a resistance rate of 88%. 40.9% of strains show an ESBL phenotype and 16.6% were MBL. An overall prevalence of 74% (40/54) and respectively rates of 29.6%, 27.7% and 16.7% for blaSHV, blaCTX and blaTEM genes were observed. SHV, CTX, CTX/SHV/TEM, CTX/TEM, SHV/TEM and CTX/SHV were different ESBL genotypes observed. ESBL-producing enterobacteria isolation worried about the future of antimicrobial therapy in the Republic of Congo. This is a public health problem that requires careful monitoring and implementation of a policy of rational antibiotics use.
基金supported by grants from the Novo Nordisk Foundation(NNF20CC0035580,NNF16OC0021746)。
文摘In silico genome mining provides easy access to secondary metabolite biosynthetic gene clusters(BGCs)encoding the biosynthesis of many bioactive compounds,which are the basis for many important drugs used in human medicine.However,the association between BGCs and other functions encoded in the genomes of producers have remained elusive.Here,we present a systems biology workflow that integrates genome mining with a detailed pangenome analysis for detecting genes associated with a particular BGC.We analyzed 3,889 enterobacterial genomes and found 13,266 BGCs,represented by 252 distinct BGC families and 347 additional singletons.A pangenome analysis revealed 88 genes putatively associated with a specific BGC coding for the colon cancer-related colibactin that code for diverse metabolic and regulatory functions.The presented workflow opens up the possibility to discover novel secondary metabolites,better understand their physiological roles,and provides a guide to identify and analyze BGC associated gene sets.
基金Financial support was provided by State Key Laboratory of Utilization of Woody Oil Resource(Grant no.2019XK2002)National Natural Science Foundation of China(Grant no.32202004,31972172)+2 种基金Natural Science Foundation of Jiangsu Province(Grant no.BK20201417)Jiangsu Province Research Fund(Grant no.JNHB-131)Starting Research Fund of Yantai University(Grant No.2222006)。
文摘The discovery and application of antibiotics in animal feeds have boomed the development of intensive animal husbandry in the last century,until the emergence of antibiotics-resistant bacteria.To alleviate the risks aroused by antibiotics-resistant bacteria,effective antibiotic substitutes are urgently needed to replace antibiotics.Essential oils(EOs)derived from plants are illustrated as the promising antibiotic substitutes used in animal feeds,as same as their current views for poultry and livestock industries in the future.It has been widely demonstrated that the phytochemicals in EOs show multiple biofunctionability and are less likely to induce resistance in bacteria.The beneficial effects of EOs feed supplementation on the intestinal inflammation,intestinal flora,immunity,digestion,and growth performances have been already extensively examined.However,the cost-effectiveness,odor,volatility,instability and bioavailability are the challenges in effectively utilizing EOs in animal intestines.Based on previous researches,and these challenges can partially be resolved by microencapsulation and nanotechnology are promising techniques to deal with these challenges.This article presents the feasibility and foundation of EOs application as antibiotic substitutes in animal feeds,and illustrates the mechanisms,functional performances and superiority of EOs compared with antibiotics.
文摘A previous study was conducted on the synthesis and antibacterial evaluation of Mannich bases of 2-(thioalkyl)-1H-methylbenzimidazole derivatives. The results of this study showed that certain 2-(thioalkyl)-1H-methylbenzimidazole and 2-(thioalkyl)-methyl-1-(piperidin-1-ylmethyl)benzimidazole derivatives possess antibacterial activities against clinical strains, such as Escherichia coli, Klebsiella pneumonia (Gram-negative bacteria), Staphylococcus aureus and Pseudomonas aeruginosa (Gram-positive bacteria). Following these favorable results, we extended the antibacterial evaluation of this series of molecules to environmental strains. The aim of this study was to assess the impact of the methyl-piperidine group fixed at position-1 of this new series of benzimidazoles on the antibacterial activity of environmental strains. In addition, we first evaluated the antibacterial activity of four 2-(thioalkyl)methylbenzimidazole derivatives and then that of five 2-(thioalkyl)-methyl-1-(piperidin-1-ylmethyl) benzimidazole derivatives. This study allowed us to show that compounds 1d and 1e could inhibit bacterial growth in vitro of the Enterobacteria P1 strain with inhibition diameters of 17.3 ± 0.6 mm and 10 ± 0.0 mm, respectively. However, addition of methyl-piperidinyl in this series showed that five (5) of 2-(thioalkyl)-methyl-1-(piperidin-1-ylmethyl) benzimidazole derivatives had an inhibitory effect on the in vitro growth of bacterial strains used except on Enterobacteria P2 with inhibition diameters between 10.0 ± 0.8 mm and 27.9 ± 1.4 mm. The introduction of the methyl-piperidinyl group at the 1-position of 2-(thioalkyl)-1H-methylbenzimidazole derivatives greatly improved the antibacterial activity against environmental bacteria such as Escherichia coli A1, A2, A3, and A4 and two Enterobacteria P1.
文摘Neonatal thrombocytopenia accounts for 20% of neonates hospitalized in the neonatal intensive care unit (NICU) at DPH. The etiologies are multiple, but bacterial infection is the third leading cause of neonatal mortality worldwide. We therefore set out to assess the frequency of neonatal thrombocytopenia associated or not with bacterial infection in the NICU. We conducted a retrospective and prospective study with the DPH NICU, over 10 months (August 2018 and April 2019). Thrombocytopenia encountered in the NICUs, were the subject of research into bacteriological, inflammatory, and epidemiological parameters using Inlog laboratory data processing software. During this period, 1280 babies were hospitalized, 94 of whom underwent thrombocytopenia, corresponding to 7.34%, with a sex ratio of 0.92. The number of babies presenting with thrombocytopenia during the first week of hospitalization was 72, accounting for 76.6%. The clinical context was usually low birth weight in 30.8% of cases and perinatal asphyxia (25%). Thrombocytopenia ranged from 2000 to 137,000 with an average of 69,520/mm3. Among these thrombocytopenias, 64 cases (68%) were below 100,000 mm3 and 44 cases had a CRP >5 mg/l. A total of 30 bacteria were isolated, including 23 Enterobacteria, 2 Streptococci, and 1 Acinetobacter. Among these enterobacteria, 14 were multidrug-resistant (MDR). Thrombocytopenia associated with a multidrug-resistant bacterial infection is a real challenging management.