Transplantation of human placental chorionic plate-derived mesenchymal stem cells for repair of neurological damage in neonatal hypoxic-ischemic encephalopathy

Neonatal hypoxic-ischemic encephalopathy is often associated with permanent cerebral palsy,neurosensory impairments,and cognitive deficits,and there is no effective treatment for complications related to hypoxic-ischemic encephalopathy.The therapeutic potential of human placental chorionic plate-derived mesenchymal stem cells for various diseases has been explored.However,the potential use of human placental chorionic plate-derived mesenchymal stem cells for the treatment of neonatal hypoxic-ischemic encephalopathy has not yet been investigated.In this study,we injected human placental chorionic plate-derived mesenchymal stem cells into the lateral ventricle of a neonatal hypoxic-ischemic encephalopathy rat model and observed significant improvements in both cognitive and motor function.Protein chip analysis showed that interleukin-3 expression was significantly elevated in neonatal hypoxic-ischemic encephalopathy model rats.Following transplantation of human placental chorionic plate-derived mesenchymal stem cells,interleukin-3 expression was downregulated.To further investigate the role of interleukin-3 in neonatal hypoxic-ischemic encephalopathy,we established an in vitro SH-SY5Y cell model of hypoxic-ischemic injury through oxygen-glucose deprivation and silenced interleukin-3 expression using small interfering RNA.We found that the activity and proliferation of SH-SY5Y cells subjected to oxygen-glucose deprivation were further suppressed by interleukin-3 knockdown.Furthermore,interleukin-3 knockout exacerbated neuronal damage and cognitive and motor function impairment in rat models of hypoxic-ischemic encephalopathy.The findings suggest that transplantation of hpcMSCs ameliorated behavioral impairments in a rat model of hypoxic-ischemic encephalopathy,and this effect was mediated by interleukin-3-dependent neurological function.

Research on the Application Framework of Intelligent Technologies to Promote Teachers’Classroom Teaching Behavior Evaluation

With the advantages of real-time analysis and visual evaluation results,intelligent technology-enabled teaching behavior evaluation has gradually become a powerful means to help teachers adjust teaching behaviors and improve teaching quality.However,at present,the evaluation of intelligent teachers’behaviors is still in the preliminary exploration stage,and the application research is not deep enough.This paper analyzes the application of intelligent technology in the evaluation of teachers’classroom teaching behaviors from the perspectives of evaluation data,methods,and results.Voice print recognition technology is used to recognize the teachers’identities and track the speech in the classroom videos,and the videos are segmented.Then,the evaluation framework of teachers’classroom teaching behaviors is constructed using three dimensions of emotion,posture,and position preference.Finally,evaluation results are presented to teachers in a more intuitive and easy-to-understand visual way,to help teachers reflect on teaching.This paper aims to promote the transformation of teachers’classroom teaching behavior evaluation toward an intelligent,efficient,and sustainable direction through current research.