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Iron and ferritin effects on intensive care unit mortality:A metaanalysis 被引量:1
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作者 Deng-Can Yang Bo-Jun Zheng +1 位作者 Jian Li Yi Yu 《World Journal of Clinical Cases》 SCIE 2024年第16期2803-2812,共10页
BACKGROUND The effect of serum iron or ferritin parameters on mortality among critically ill patients is not well characterized.AIM To determine the association between serum iron or ferritin parameters and mortality ... BACKGROUND The effect of serum iron or ferritin parameters on mortality among critically ill patients is not well characterized.AIM To determine the association between serum iron or ferritin parameters and mortality among critically ill patients.METHODS Web of Science,Embase,PubMed,and Cochrane Library databases were searched for studies on serum iron or ferritin parameters and mortality among critically ill patients.Two reviewers independently assessed,selected,and abstracted data from studies reporting on serum iron or ferritin parameters and mortality among critically ill patients.Data on serum iron or ferritin levels,mortality,and demographics were extracted.RESULTS Nineteen studies comprising 125490 patients were eligible for inclusion.We observed a slight negative effect of serum ferritin on mortality in the United States population[relative risk(RR)1.002;95%CI:1.002-1.004].In patients with sepsis,serum iron had a significant negative effect on mortality(RR=1.567;95%CI:1.208-1.925).CONCLUSION This systematic review presents evidence of a negative correlation between serum iron levels and mortality among patients with sepsis.Furthermore,it reveals a minor yet adverse impact of serum ferritin on mortality among the United States population. 展开更多
关键词 IRON ferritin MORTALITY Critically ill Meta analysis
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Ferritin and mortality in hemodialysis patients with COVID-19:A systematic review and meta-analysis
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作者 Ni Wayan Anantika Riani I Gde Raka Widiana Yenny Kandarini 《Asian Pacific Journal of Tropical Medicine》 SCIE CAS 2024年第1期4-11,共8页
Objective:To investigate the difference in serum ferritin levels between deceased and surviving regular hemodialysis patients with COVID-19.Methods:We conducted a systematic search across four databases following the ... Objective:To investigate the difference in serum ferritin levels between deceased and surviving regular hemodialysis patients with COVID-19.Methods:We conducted a systematic search across four databases following the PRISMA statement guidelines.Studies reporting ferritin levels and mortality of regular hemodialysis patients with COVID-19 were included.Employing the random-effects model,we performed a meta-analysis to determine the mean difference in serum ferritin levels between the studied groups,along with their corresponding 95%confidence intervals.The meta-analysis was carried out using Review Manager 5.4 and Stata 16.Results:A total of 1013 patients from seven studies were included in this study.Our meta-analysis showed higher mean serum ferritin in the deceased compared to surviving regular hemodialysis patients with COVID-19,with a mean difference of 449.43 ng/mL[95%CI(244.07,654.80),P<0.0001;I2=58%,P=0.003].Conclusions:Our study found a higher mean of serum ferritin levels in the deceased compared to surviving regular hemodialysis patients with COVID-19. 展开更多
关键词 ferritin MORTALITY HEMODIALYSIS COVID-19 INFLAMMATION
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Serum ferritin and the risk of early-onset colorectal cancer
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作者 Adam L Urback Kylee Martens +5 位作者 Hannah Stowe McMurry Emerson Y Chen Caitlin Citti Anil Sharma Adel Kardosh Joseph J Shatzel 《World Journal of Gastrointestinal Oncology》 SCIE 2024年第8期3496-3506,共11页
BACKGROUND The incidence of early-onset colorectal cancer(EO-CRC)is rising in the United States,and is often diagnosed at advanced stages.Low serum ferritin is often incidentally discovered in young adults,however,the... BACKGROUND The incidence of early-onset colorectal cancer(EO-CRC)is rising in the United States,and is often diagnosed at advanced stages.Low serum ferritin is often incidentally discovered in young adults,however,the indication for endoscopy in EO-CRC is unclear.AIM To compare serum ferritin between patients with EO-CRC and healthy controls(HCs),and examine the association of serum ferritin in EO-CRC with patient-and disease-specific characteristics.METHODS A retrospective study of patients<50 years with newly-diagnosed EO-CRC was conducted from 1/2013-12/2023.Patients were included if serum ferritin was measured within 2 years prior to 1 year following CRC histologic diagnosis.To supplement the analysis,a cohort of HCs meeting similar inclusion and exclusion criteria were identified for comparison.A sensitivity analysis including only patients with serum ferritin obtained at or before diagnosis was separately performed to minimize risk of confounding.RESULTS Among 85 patients identified with EO-CRC(48 females),the median serum ferritin level was 26 ng/mL(range<1-2759 ng/mL).Compared to HCs(n=80211),there were a higher proportion of individuals with EO-CRC with serum ferritin<20 ng/mL(female 65%,male 40%)versus HCs(female 32.1%,male 7.2%)age 29-39 years(P=0.002 and P<0.00001,respectively).Stage IV disease was associated with significantly higher serum ferritin compared to less advanced stages(P<0.001).Serum ferritin obtained before or at the time of diagnosis was lower than levels obtained after diagnosis.Similar findings were confirmed in the sensitivity analysis.CONCLUSION Severe iron deficiency may indicate an increased risk of EO-CRC,particularly at earlier stages.Further studies defining the optimal serum ferritin threshold and routine incorporation of serum ferritin in screening algorithms is essential to develop more effective screening strategies for EO-CRC. 展开更多
关键词 EARLY-ONSET Young-onset Colorectal cancer Age ferritin Iron deficiency
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Association between Serum Ferritin Levels and Metabolic-associated Fatty Liver Disease in Adults:a Cross-sectional Study Based on the NHANES
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作者 Jiang-hui LI Xue-yao MA +3 位作者 Yun YI Lu-rao LI Zhi-yong XU Ying CHANG 《Current Medical Science》 SCIE CAS 2024年第3期494-502,共9页
Objective Ferritin,initially acting as an iron-storage protein,was found to be associated with metabolic diseases.Our study was designed to investigate the association between serum ferritin and metabolic-associated f... Objective Ferritin,initially acting as an iron-storage protein,was found to be associated with metabolic diseases.Our study was designed to investigate the association between serum ferritin and metabolic-associated fatty liver disease(MAFLD)using data from the National Health and Nutrition Examination Survey(NHANES)of the United State of America.Methods A cross-sectional study was conducted,enrolling a total of 2145 participants from the NHANES in the 2017–2018 cycles.Hepatic steatosis and liver fibrosis were assessed by ultrasound images and several non-invasive indexes.Multiple regression analysis was conducted to determine the associations between serum ferritin concentration and MAFLD and liver fibrosis.Results The analysis revealed that participants with higher serum ferritin levels(Q3 and Q4 groups)had a higher prevalence of MAFLD than those with the lowest serum ferritin levels[Q3 vs.Q1:OR=2.17(1.33,3.53),P<0.05 in fatty liver index(FLI);Q4 vs.Q1:OR=3.13(1.91,5.13),P<0.05 in FLI].Additionally,participants with the highest serum ferritin levels(Q4 group)displayed a higher prevalence of liver fibrosis[Q4 vs.Q1:OR=2.59(1.19,5.62),P<0.05 in liver stiffness measurement;OR=5.06(1.12,22.94),P<0.05 in fibrosis-4 index],with significantly increased risk observed in participants with concomitant diabetes[OR=7.45(1.55,35.72),P=0.012].Conclusion Our study revealed that elevated serum ferritin levels are associated with a higher prevalence of MAFLD and advanced liver fibrosis in patients.Elevated serum ferritin levels combined with diabetes are important risk factors for liver fibrosis. 展开更多
关键词 serum ferritin liver fibrosis metabolic-associated fatty liver disease National Health and Nutrition Examination Survey
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Serum tumor markers (carcinoembryonic antigen, carbohydrate antigen 19-9, carbohydrate antigen 72-4, carbohydrate antigen 24-2, ferritin) and gastric cancer prognosis correlation
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作者 Jie-Wen Zhu Ling-Zhen Gong Qian-Wen Wang 《World Journal of Gastrointestinal Surgery》 SCIE 2024年第9期2808-2814,共7页
BACKGROUND Gastric cancer is a kind of malignant tumor which is prevalent all over the world.Although some progress has been made in the treatment of gastric cancer,its prognosis is still not optimistic,so it is of gr... BACKGROUND Gastric cancer is a kind of malignant tumor which is prevalent all over the world.Although some progress has been made in the treatment of gastric cancer,its prognosis is still not optimistic,so it is of great significance to find reliable prog-nostic indicators to guide the treatment and management of patients with gastric cancer.AIM To explore the relationship between serum levels of five biomarkers[carcinoem-bryonic antigen(CEA),carbohydrate antigen(CA)19-9,CA72-4,CA24-2,and ferritin]and prognosis in patients with gastric cancer.METHODS This study included 200 patients with gastric adenocarcinoma,and conducted an in-depth analysis of their baseline characteristics,relationship between tumor markers and staging,and prognosis.The study found that CA19-9 has a signi-ficant correlation with tumor stage,the average levels of CA24-2,CEA,CA72-4 and ferritin were slightly increased disregarding the stage of tumor.Survival analysis showed that increases in CEA,CA19-9,CA24-2,and ferritin were all associated with shortened overall survival of patients.Further multivariate ana-lysis revealed that elevated serum CA72-4 levels were an inde-pendent adverse prognostic factor.RESULTS This study reveals that there is a significant correlation between the expression levels of serum tumor markers CEA,CA19-9,CA72-4,CA24-2 and ferritin in patients with gastric cancer and prognosis,and can be used as important indicators for prognostic evaluation of gastric cancer.In particular,markers that appear abnormally elevated initially may help identify gastric cancer patients with poor prognosis.CONCLUSION Serum CEA and CA19-9 play an important role in the prognosis assessment of gastric cancer,and are effective tools to guide clinical practice and optimize individualized treatment strategies for gastric cancer patients. 展开更多
关键词 Gastric cancer PROGNOSIS Carcinoembryonic antigen Carbohydrate antigen 19-9 Carbohydrate antigen 72-4 Carbohydrate antigen 24-2 ferritin Serum markers Retrospective study
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AFP、PIVKA-Ⅱ、Ferritin联合应用在肝癌诊断及疗效监测中的效能评价 被引量:1
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作者 倪凯茹 高晨慧 +1 位作者 颜士健 洪智慧 《标记免疫分析与临床》 CAS 2024年第3期444-449,538,共7页
目的评价单独及联合应用甲胎蛋白(AFP)、异常凝血酶原(PIVKA-Ⅱ)、铁蛋白(Ferritin)指标在肝癌诊断及预后评估中的效能。方法回顾性分析10个月内在苏州大学附属第二医院核医学科就诊的体检、门诊、住院患者的AFP、PIVKA-Ⅱ、Ferritin检... 目的评价单独及联合应用甲胎蛋白(AFP)、异常凝血酶原(PIVKA-Ⅱ)、铁蛋白(Ferritin)指标在肝癌诊断及预后评估中的效能。方法回顾性分析10个月内在苏州大学附属第二医院核医学科就诊的体检、门诊、住院患者的AFP、PIVKA-Ⅱ、Ferritin检测数值,按照临床诊断信息分为健康体检组(74例)、肝炎肝囊肿组(63例)、肝硬化组(40例)、腺瘤增生组(76例)及肝癌组(788例),比较各指标在各肝相关分组中的表达水平差异。通过Passing-Bablok回归性分析及Kappa分析评价AFP与PIVKA-Ⅱ的应用特性。通过ROC曲线及Kappa分析比较各指标单独及联合应用在诊断肝恶性肿瘤的效能。比较各指标对肝恶性肿瘤预后的评估价值。结果3指标在各分组中的表达差异明显,AFP在健康人群阳性检出率为4.05%,在肝癌组阳性检出率为57.61%;PIVKA-Ⅱ在健康体检组阳性检出率为4.05%,在肝癌组阳性检出率为64.47%;Ferritin在健康体检组阳性检出率为12.16%,在肝癌组阳性检出率为34.90%。分析AFP与PIVKA-Ⅱ指标相关性,Y=0.0786 X+1.5675,线性度偏差显著;一致性分析,Kappa值为0.381,一致性一般。多指标联合应用在肝癌组的诊断效能高于单一指标,ROC曲线下面积为0.802,在腺瘤增生+肝癌组中诊断的ROC曲线下面积为0.771;在肝癌诊断中,AFP、Ferritin、PIVKA-Ⅱ3项联检的灵敏度为88.96%,特异性为80.24%,高于单项目检测的灵敏度,在良恶性肿瘤诊断中,3项联检的灵敏度为82.52%,特异性为78.53%。3指标在肝癌治疗前后差异明显,AFP在治疗前后中位数由4.11ng/mL下调至3.2ng/mL,Ferritin在治疗前后中位数由244ng/mL下调至223ng/mL,PIVKA-Ⅱ在治疗前后中位数由161mIU/mL下调至24.78mIU/mL;秩和检验分析治疗前后数值差异,AFP:P<0.0247;Ferritin:P<0.4842;PIVKA-Ⅱ:P<0.0001,AFP、PIVKA-Ⅱ指标变化有更强的临床一致性和预后指导价值。结论3项传统肝癌检测指标在良恶性疾病中表达差异明显,其中两个核心指标AFP与PIVKA-Ⅱ一致性较差,但互补性强,联检可以有效提高阳性检出率。相较于腺瘤增生+肝癌综合诊断,多指标联检对单纯肝癌的诊断效能更高。AFP与PIVKA-Ⅱ的疗效监测价值更高。 展开更多
关键词 甲胎蛋白 异常凝血酶原 铁蛋白 肝癌 诊断 疗效监测
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Magnetic resonance imaging focused on the ferritin heavy chain 1 reporter gene detects neuronal differentiation in stem cells 被引量:1
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作者 Xiao-Ya He Yi-Rui Zhou +4 位作者 Tong Mu Yi-Fan Liao Li Jiang Yong Qin Jin-Hua Cai 《Neural Regeneration Research》 SCIE CAS CSCD 2023年第7期1563-1569,共7页
The neuronal differentiation of mesenchymal stem cells offers a new strategy for the treatment of neurological disorders.Thus,there is a need to identify a noninvasive and sensitive in vivo imaging approach for real-t... The neuronal differentiation of mesenchymal stem cells offers a new strategy for the treatment of neurological disorders.Thus,there is a need to identify a noninvasive and sensitive in vivo imaging approach for real-time monitoring of transplanted stem cells.Our previous study confirmed that magnetic resonance imaging,with a focus on the ferritin heavy chain 1 reporter gene,could track the proliferation and differentiation of bone marrow mesenchymal stem cells that had been transduced with lentivirus carrying the ferritin heavy chain 1 reporter gene.However,we could not determine whether or when bone marrow mesenchymal stem cells had undergone neuronal differentiation based on changes in the magnetic resonance imaging signal.To solve this problem,we identified a neuron-specific enolase that can be differentially expressed before and after neuronal differentiation in stem cells.In this study,we successfully constructed a lentivirus carrying the neuron-specific enolase promoter and expressing the ferritin heavy chain 1 reporter gene;we used this lentivirus to transduce bone marrow mesenchymal stem cells.Cellular and animal studies showed that the neuron-specific enolase promoter effectively drove the expression of ferritin heavy chain 1 after neuronal differentiation of bone marrow mesenchymal stem cells;this led to intracellular accumulation of iron and corresponding changes in the magnetic resonance imaging signal.In summary,we established an innovative magnetic resonance imaging approach focused on the induction of reporter gene expression by a neuron-specific promoter.This imaging method can be used to noninvasively and sensitively detect neuronal differentiation in stem cells,which may be useful in stem cell-based therapies. 展开更多
关键词 ferric ammonium citrate ferritin heavy chain 1 gene magnetic resonance imaging mesenchymal stem cells molecular imaging neuronal differentiation neuron-like cells NEURONS neuron-specific enolase PROMOTER
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Effect of Glycosylation on the Physicochemical Properties,Structure and Iron Bioavailability of Ferritin Extracted from Tegillarca granosa
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作者 SUN Bolun TAN Beibei +4 位作者 LI Gaoshang YANG Changjie ZHANG Panxue LI Chao YANG Wenge 《Journal of Ocean University of China》 SCIE CAS CSCD 2023年第4期1068-1078,共11页
Iron deficiency anemia(IDA)is a major global health problem.Tegillarca granosa has been considered as an excellent source of iron given its high content of iron-binding protein,ferritin.The aim of the present study wa... Iron deficiency anemia(IDA)is a major global health problem.Tegillarca granosa has been considered as an excellent source of iron given its high content of iron-binding protein,ferritin.The aim of the present study was to determine the physicochemical properties,protein structures,and iron uptake of ferritin extracted from T.granosa,and to evaluate the potential impacts of chitosan glycosylation on these characteristics.Based on Box-Behnken design and response surface methodology,the optimal conditions for glycosylation included a ferritin/chitosan mass ratio of 4:1,a pH of 5.5,a reaction time of 10 min,and a reaction temperature of 50℃.Glycosylation caused decreased surface hydrophobicity and elevated water-holding capacity of ferritin due to the introduction of hydrophilic groups.Additionally,glycosylation improved antioxidant capacity of ferritin by 20.69%–189.66%,likely owing to the protons donated by saccharide moiety to terminate free radical chain reaction.The in vitro digestibility of ferritin was elevated by 22.56%–104.85%after glycosylation,which could be associated with lessβ-sheet content in secondary structure that made the glycosylated protein less resistant to enzymatic digestion.The results of the iron bioavailability in Caco-2 cells revealed that ferritin(78.85–231.77 ngmg^(−1))exhibited better iron bioavailability than FeSO4(51.48–114.37 ngmg^(−1))and the values were further elevated by glycosylation with chitosan(296.23–358.20 ngmg^(−1)),which may be related to the physicochemical properties of ferritin via glycosylation modification.These results provide a basis for the development of T.granosa derived ferritin and its glycosylated products,and can promote the utilization of aquatic resources. 展开更多
关键词 ferritin Tegillarca granosa GLYCOSYLATION box-behnken design iron bioavailability physicochemical properties
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Association of Serum Ferritin Levels and Thyroid Hormones
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作者 Hari Krishnan Krishnamurthy Swarnkumar Reddy +6 位作者 Vasanth Jayaraman Karthik Krishna Qi Song Karenah E. Rajasekaran Tianhao Wang Kang Bei John J. Rajasekaran 《Open Journal of Clinical Diagnostics》 2023年第3期68-79,共12页
Thyroid metabolism is orchestrated by the action of various minerals and trace elements including iron, iodine, selenium, and zinc. Iron deficiency, specifically deficiency in serum ferritin levels, is one of the comm... Thyroid metabolism is orchestrated by the action of various minerals and trace elements including iron, iodine, selenium, and zinc. Iron deficiency, specifically deficiency in serum ferritin levels, is one of the common causes of thyroid dysfunction. Our objective was to evaluate the relationship between serum ferritin levels and circulating thyroid hormones. For this, a retrospective analysis was performed on 16,512 individuals who tested for serum levels of ferritin and thyroid profile at Vibrant America Clinical Laboratories. Subjects were stratified based on the serum levels of ferritin. Age (p −0.03232, p < 0.0001). Analysis of Linear association by Pearson’s correlation exhibited a considerable correlation between varying serum ferritin levels with all tested thyroid hormones. The study concludes that serum ferritin levels were associated with thyroid hormone synthesis and metabolism in individuals with optimal levels of circulating ferritin. 展开更多
关键词 ferritin THYROXINE Free Triiodothyronine Free Thyroxine Thyroid Stimulating Hormone
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Hepcidin levels in hereditary hyperferritinemia:Insights into the iron-sensing mechanism in hepatocytes 被引量:1
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作者 Jayantha Arnold Arvind Sangwaiya +4 位作者 Vijay Manglam Mark Thursz Caroline Beaumont Caroline Kannengiesser Mark Busbridge 《World Journal of Gastroenterology》 SCIE CAS CSCD 2010年第28期3541-3545,共5页
AIM:To study the role of hepcidin in hereditary hyperferritinemia cataract syndrome(HHCS). METHODS:Six patients from two families with HHCS, confirmed by genetic analysis showing A to G mutation at position+40 in the ... AIM:To study the role of hepcidin in hereditary hyperferritinemia cataract syndrome(HHCS). METHODS:Six patients from two families with HHCS, confirmed by genetic analysis showing A to G mutation at position+40 in the L-ferritin gene,were recruited to undergo serum hepcidin and prohepcidin measurements using radioimmunoassay and enzyme linked immunoassay,respectively,and measurements were compared with levels in serum from 25 healthy volunteers(14 females),mean age 36±11.9 years.RESULTS:The serum hepcidin and prohepcidin levels in patients with HHCS were 19.1±18.6 and 187± 120.9 ng/mL,respectively.Serum ferritin was 1716.3± 376μg/L.Liver biopsy in one patient did not show any evidence of iron overload.Serum hepcidin and prohepcidin values in healthy controls(HCs)were 15.30±15.71 and 236.88±83.68 ng/mL,respectively,while serum ferritin was 110±128.08μg/L.There was no statistical difference in serum hepcidin level between the two cohorts(19.1±18.6 ng/mL vs 15.30±15.71 ng/mL,P= 0.612)using two-tailed t-test. CONCLUSION:Serum hepcidin levels in HHCS patients is similar to that in HCs.Our study suggests that circulating ferritin is not a factor influencing hepcidin synthesis and does not have a role in the iron-sensing mechanism in hepatocytes. 展开更多
关键词 Hereditary hyperferritinemia Hereditary hyperferritinemia cataract syndrome HEPCIDIN Hepcidin assay Iron-sensing mechanism Iron responsive element ferritin
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仿刺参铁蛋白ferritin基因的序列分析及表达 被引量:13
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作者 杨爱馥 周遵春 +6 位作者 孙大鹏 董颖 姜北 汪笑宇 陈仲 关晓燕 王摆 《水产学报》 CAS CSCD 北大核心 2010年第6期710-717,共8页
通过构建仿刺参cDNA文库,获得铁蛋白全长cDNA序列。该基因序列全长792bp,5′-非翻译区长133bp,开放阅读框长522bp,编码173个氨基酸,3′-UTR长137bp;预测蛋白分子量为20ku。5′-UTR具有一个高度保守的铁离子应答元件。仿刺参ferritin氨... 通过构建仿刺参cDNA文库,获得铁蛋白全长cDNA序列。该基因序列全长792bp,5′-非翻译区长133bp,开放阅读框长522bp,编码173个氨基酸,3′-UTR长137bp;预测蛋白分子量为20ku。5′-UTR具有一个高度保守的铁离子应答元件。仿刺参ferritin氨基酸序列具备脊椎动物ferritin的亚铁氧化酶活性中心所特有的保守结构。该序列与海参的同源性最高,达84%,与其它无脊椎动物如:海星、鲍、牡蛎、海葵、线虫、小龙虾和果蝇的同源性为74%~34%;与脊椎动物ferritin重链亚基同源性高于轻链亚基。系统进化分析表明,仿刺参的ferritin与大部分无脊椎动物聚为一支。利用半定量RT-PCR检测,ferritin mRNA在仿刺参未受精卵、受精卵、多细胞期、囊胚期、原肠期、小耳状幼体、中耳状幼体、大耳状幼体、樽型幼体、五触手幼体、稚参11个发育阶段和幼参的体壁、体腔细胞、肠道和呼吸树中均表达。Quantitative real-time PCR结果显示,ferritin mRNA在未受精卵至原肠期表达量低,从小耳状幼体至稚参表达量显著增高;在幼参的不同组织中,ferritin mRNA在呼吸树中的表达量显著低于其他3种组织;幼参注射LPS后,4种组织中ferritin mRNA表达量与注射前无显著差异。 展开更多
关键词 仿刺参 铁蛋白ferritin基因 序列分析 基因表达 LPS刺激
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干露胁迫对脊尾白虾(Exopalaemon carinicauda)HSP70和ferritin基因表达的影响 被引量:14
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作者 王琦 李健 +2 位作者 李吉涛 潘鲁青 杨爱国 《海洋与湖沼》 CAS CSCD 北大核心 2013年第2期409-414,共6页
采用常规急性实验方法,研究了不同干露胁迫条件对脊尾白虾(Exopalaemon carinicauda)成活率的影响。结果表明,保持虾体湿润并用冰块降温的P组干露胁迫12h后成活率为75%,显著高于其它各实验组(P<0.05)。采用实时荧光定量PCR方法研究... 采用常规急性实验方法,研究了不同干露胁迫条件对脊尾白虾(Exopalaemon carinicauda)成活率的影响。结果表明,保持虾体湿润并用冰块降温的P组干露胁迫12h后成活率为75%,显著高于其它各实验组(P<0.05)。采用实时荧光定量PCR方法研究了不同干露胁迫条件对脊尾白虾血细胞和肝胰腺组织中热休克蛋白70(HSP70)和ferritin基因表达的影响。干露胁迫能诱导脊尾白虾血细胞、肝胰腺HSP70基因的表达上调,肝胰腺中的高表达时间相对血细胞中出现的较早;干露胁迫能诱导湿润低温P组脊尾白虾血细胞和肝胰腺ferritin基因的表达上调,并显著高于对照组(P<0.05),而且各组织ferritin基因表达的上调时间具有差异性,血细胞最先上调。其余实验组脊尾白虾各组织ferritin基因表达均下调,并显著低于对照组(P<0.05)。上述结果表明,在脊尾白虾受干露胁迫的耐受范围内,HSP70和ferritin基因发挥抗氧化功能。 展开更多
关键词 脊尾白虾 干露 成活率 HSP70 ferritin
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干旱胁迫条件下发菜Ferritin差异表达与基因克隆 被引量:4
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作者 梁文裕 焦广飞 +2 位作者 周有文 张亚萍 陈伟 《植物科学学报》 CAS CSCD 北大核心 2012年第1期72-78,共7页
发菜(Nostoc flagelliforme)是一种陆生固氮蓝藻,具有强烈的旱生生态适应性。运用双向电泳技术、凝胶图像分析、MALDI-TOF-TOF/MS质谱鉴定和数据库检索,发现发菜Ferritin在干旱胁迫条件下表达量逐渐降低。根据鉴定的Ferritin已知氨基酸... 发菜(Nostoc flagelliforme)是一种陆生固氮蓝藻,具有强烈的旱生生态适应性。运用双向电泳技术、凝胶图像分析、MALDI-TOF-TOF/MS质谱鉴定和数据库检索,发现发菜Ferritin在干旱胁迫条件下表达量逐渐降低。根据鉴定的Ferritin已知氨基酸序列设计简并性引物克隆该基因,获得了长度为540 bp的DNA,GenBank登陆号为HM854287。序列比较显示该基因具有较高的保守性,蛋白质二级结构主要由α螺旋和随机卷曲构成。RT-PCR分析表明,Ferritin mRNA在干旱胁迫条件下表达量逐渐降低,与Ferritin的表达趋势一致。将Ferritin基因在大肠杆菌中表达,获得符合预期的外源重组蛋白(22.4 kD)。实验结果可为进一步研究发菜耐旱的分子机理及探讨发菜对极端干旱环境的适应和保护机制奠定基础。 展开更多
关键词 发菜 ferritin 差异表达 基因克隆 RT-PCR 原核表达
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青岛文昌鱼铁蛋白ferritin基因的序列分析、同源性比较及二级结构预测 被引量:5
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作者 李忻怡 张伟 +3 位作者 林浴霜 于善清 王振光 张红卫 《山东大学学报(理学版)》 CAS CSCD 北大核心 2003年第5期116-120,共5页
对青岛文昌鱼神经胚cDNA文库进行系统测序 ,分析测序结果 ,获得 4个铁蛋白ferritin的EST ,经过拼接得到含有完整读框的文昌鱼铁蛋白ferritin基因cDNA序列并据此演绎出其氨基酸序列 ,对演绎的文昌鱼铁蛋白ferritin的一级结构进行了分析 ... 对青岛文昌鱼神经胚cDNA文库进行系统测序 ,分析测序结果 ,获得 4个铁蛋白ferritin的EST ,经过拼接得到含有完整读框的文昌鱼铁蛋白ferritin基因cDNA序列并据此演绎出其氨基酸序列 ,对演绎的文昌鱼铁蛋白ferritin的一级结构进行了分析 ,对其二级结构进行了预测 ,并与多种真核生物中的铁蛋白ferritin进行同源性比较 ,为认识文昌鱼铁蛋白ferritin和研究该分子的进化和功能提供了资料 . 展开更多
关键词 文昌鱼 ferritin 克隆
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葡萄铁蛋白基因Ferritin的鉴定、克隆及其在果实发育不同时期对氨基酸铁复合肥喷施的响应
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作者 宋志忠 王建萍 +5 位作者 史圣朋 曹晶雯 刘万好 徐维华 肖慧琳 唐美玲 《中国农业科学》 CAS CSCD 北大核心 2023年第18期3629-3641,共13页
【目的】铁蛋白Ferritin在植物生长发育过程中发挥重要作用,其在果树中的生物学功能尚未见报道。克隆葡萄Ferritin家族基因并揭示其在果实发育不同时期的表达模式及其对叶面喷施氨基酸-铁(Fe)复合肥处理的响应差异,可为研究果树铁素营... 【目的】铁蛋白Ferritin在植物生长发育过程中发挥重要作用,其在果树中的生物学功能尚未见报道。克隆葡萄Ferritin家族基因并揭示其在果实发育不同时期的表达模式及其对叶面喷施氨基酸-铁(Fe)复合肥处理的响应差异,可为研究果树铁素营养与代谢的分子机制提供理论依据。【方法】通过同源克隆法从‘马瑟兰’中克隆Ferritin家族基因,利用生物信息学手段分析葡萄Ferritin及其编码蛋白的详细特征;设置叶面喷施氨基酸-Fe复合肥处理,利用荧光实时定量PCR技术分析Ferritin在葡萄果实发育不同时期的表达特征及其对叶面喷施处理的差异响应。【结果】在葡萄基因组中检索并克隆获得4个Ferritin家族基因,命名为VvFer1—VvFer4,分布于第6、8和13号三条染色体上,均含有7个长度不一的内含子,且主要定位于叶绿体和细胞核。本文所选16种植物Ferritin蛋白序列的一致性高达61.48%,系统发育树分析表明同一属的Ferritin同源蛋白如十字花科的拟南芥和芜菁,茄科的烟草和马铃薯,豆科的大豆、落花生和鹰嘴豆,大戟科的橡胶树、木薯和蓖麻,蔷薇科的苹果、桃和草莓,遗传进化关系较近,葡萄VvFer3和茄科同源蛋白遗传关系最近。葡萄VvFer基因在五年生‘马瑟兰’果实发育不同时期、不同组织中的表达水平差异较大,其中,VvFer3在不同组织中的表达量均最高,最大值出现在硬核期至成熟期的果实中,其次是VvFer2和VvFer4。‘马瑟兰’葡萄发育不同时期果实中的Fe含量略有差异,从幼果期开始逐渐增加,并在转色期果实中达到最高值,从转色期到成熟期略有下降,但仍高于幼果期和硬核期果实中的Fe含量,叶面喷施处理显著提高了成熟期果实中的Fe含量、乌头酸酶ACO(aconitase)、硝酸还原酶NIR(nitrite reductase)和琥珀酸脱氢酶SDH(succinate dehydrogenase)的酶活性。VvFer2—4在转录水平易受叶面喷施铁肥的诱导而显著增强,且与葡萄组织/器官分布和果实发育的不同时期密切相关,VvFer2在葡萄发育整个周期仅在果实中对叶面喷施铁肥处理比较敏感,VvFer3在所有组织中仅从幼果期至转色期对叶面喷施铁肥有响应,VvFer4在葡萄发育整个周期的韧皮部和叶片中持续受叶面喷施铁肥的诱导,而在果实中仅从幼果期至转色期对叶面喷施铁肥有响应;VvFer1在本文所选组织中的表达量相对较低,但很均匀,且在转录水平对叶面喷施铁肥没有响应。【结论】从葡萄中克隆并鉴定了4个铁蛋白基因Ferritin,其在葡萄发育不同时期、不同组织中的表达水平差异较大,并在转录水平易受叶面喷施铁肥的诱导;VvFer3在葡萄所有组织中的整体表达量最高(特别是果实中最为突出),且在幼果期至转色期易受铁肥叶面喷施的调控。 展开更多
关键词 葡萄 Fe的储藏与封存 Fe蛋白 氨基酸-Fe复合肥 叶面喷施
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LASP1下调和ferritin上调在rhBMP-2诱导的比格犬BMSCs成骨分化中起重要作用 被引量:4
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作者 胡稷杰 刘亚伟 +2 位作者 何敏毅 余斌 王钢 《南方医科大学学报》 CAS CSCD 北大核心 2013年第8期1207-1212,共6页
目的建立稳定的比格犬骨髓间充质干细胞(BMSCs)诱导成骨分化模型;鉴定参与比格犬BMSCs细胞成骨分化过调控的蛋白质,探讨其可能的调控机制。方法分离培养比格犬BMSCs细胞,rhBMP-2诱导细胞成骨分化7 d,基于蛋白质双向凝胶电泳的蛋白质组... 目的建立稳定的比格犬骨髓间充质干细胞(BMSCs)诱导成骨分化模型;鉴定参与比格犬BMSCs细胞成骨分化过调控的蛋白质,探讨其可能的调控机制。方法分离培养比格犬BMSCs细胞,rhBMP-2诱导细胞成骨分化7 d,基于蛋白质双向凝胶电泳的蛋白质组学分析成骨分化组与对照组差异表达的蛋白质,对感兴趣的蛋白质LASP1、ferritin轻链和重链表达情况用Q-PCR、Western blot进行验证。结果成功诱导比格犬BMSCs成骨分化;蛋白质组学分析获得rhBMP-2诱导上调的蛋白质9个,下调的蛋白质11个。荧光定量PCR技术和Western blot对LASP1和ferritin表达情况进行了验证,发现rhBMP-2诱导7 d后,LASP1显著下调而ferritin显著上调,与蛋白质组学结果一致。结论 LASP1在调节细胞骨架活性方面发挥重要功能,而ferritin是细胞铁离子稳态维持的重要分子,提示这两种蛋白质在rhBMP-2诱导的比格犬BMSCs成骨分化过程中发挥重要作用。 展开更多
关键词 骨髓间充质干细胞 成骨分化 重组人骨形态发生蛋白-2 LASP1 ferritin
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细粒棘球蚴重组St-Eg 95-Eg.ferritin疫苗构建及生物学特性检测 被引量:3
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作者 王志昇 林源 +4 位作者 刘强 吴璟 郎多勇 李红兵 杨文 《西北农业学报》 CAS CSCD 北大核心 2014年第10期44-48,共5页
构建表达细粒棘球蚴Eg95-Eg.ferritin融合蛋白的重组口服减毒鼠伤寒沙门菌活载体疫苗株并评价其生物学特性。将细粒棘球蚴Eg95-Eg.ferritin融合基因插入到表达载体pYA3341中,构建重组质粒pYA3341-Eg95-Eg.ferritin,将重组质粒分别电转... 构建表达细粒棘球蚴Eg95-Eg.ferritin融合蛋白的重组口服减毒鼠伤寒沙门菌活载体疫苗株并评价其生物学特性。将细粒棘球蚴Eg95-Eg.ferritin融合基因插入到表达载体pYA3341中,构建重组质粒pYA3341-Eg95-Eg.ferritin,将重组质粒分别电转入减毒鼠伤寒沙门菌X3770和X4550,获得重组疫苗菌株St-Eg95-Eg.ferritin,对重组菌的稳定性、生长状态及安全性进行分析。结果表明,经PCR和酶切鉴定成功构建重组质粒pYA3341-Eg95-Eg.ferritin,Western blot检测Eg95-Eg.ferritin蛋白在重组菌中获得表达;生物学特性研究结果表明,重组质粒可稳定存在,且不影响重组菌的生长状态,小鼠口服试验证实,重组菌安全无毒性。成功构建能稳定表达细粒棘球蚴Eg95-Eg.ferritin融合蛋白的口服减毒鼠伤寒沙门菌活载体疫苗株,将为研究包虫病口服基因工程疫苗奠定基础。 展开更多
关键词 细粒棘球蚴 Eg95-Eg.ferritin 减毒鼠伤寒沙门菌 口服疫苗
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Full-length cDNA Cloning and Tissues Expression Analysis of Ferritin Gene from Acipenser sinensis 被引量:2
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作者 陈晓武 施志仪 程千千 《Zoological Research》 CAS CSCD 北大核心 2009年第2期144-150,共7页
According to the conserved sequence of the ferritin gene, a homologous sequence was obtained from the EST database through a BLAST search against the GenBank database. This sequence was amplified with the method of RT... According to the conserved sequence of the ferritin gene, a homologous sequence was obtained from the EST database through a BLAST search against the GenBank database. This sequence was amplified with the method of RT-PCR, false sequencing was corrected, and full length eDNA of the ferritin subunit from the Chinese sturgeon was obtained. After being submitted to the GenBank database, the sequence accession number EU348782 was assigned. With the length of 896 bp, this eDNA includes entire coding regions of 53 lbp, which encodes 176 amino acids (aa). The molecular weight was predicted to be 20339.9Mr and the theoretical isoelectric point 5.66. It shares 82.9% protein sequence homology with the ferritin of the Atlantic salmon. This gene is expressed in many organs of the Chinese sturgeon, for example, the liver, pancreas, muscle, brain, heart and gastric mucosa. The highest expression level was found in the pancreas and the heart, while the muscular tissue showed the lowest. Homology modeling was used to predict the 3-D structure of the protein, which included 5 alpha helices and 10 turns. The ferritin protein structure could be overlapped and showed high similarity with that of human, flog and bacteria. It was revealed that this kind of ferritin was highly conserved in structure and function. 展开更多
关键词 Acipenser sinensis ferritin BIOINFORMATICS
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细粒棘球蚴Eg95-Eg.ferritin融合基因在昆虫细胞/杆状病毒中的表达 被引量:2
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作者 李红兵 杨文 +6 位作者 陈健茂 刘强 王志辉 张昕 吴璟 郎多勇 王志昇 《宁夏医科大学学报》 2015年第2期120-124,F0004,共6页
目的利用昆虫细胞/杆状病毒系统表达细粒棘球蚴Eg95-Eg.ferritin融合蛋白,用于开发包虫病新型疫苗以及建立相关血清学诊断方法等研究。方法从细粒棘球蚴包囊中分离原头节,超声粉碎后提取总RNA为模板,通过RT-PCR扩增细粒棘球蚴Eg95和Eg.f... 目的利用昆虫细胞/杆状病毒系统表达细粒棘球蚴Eg95-Eg.ferritin融合蛋白,用于开发包虫病新型疫苗以及建立相关血清学诊断方法等研究。方法从细粒棘球蚴包囊中分离原头节,超声粉碎后提取总RNA为模板,通过RT-PCR扩增细粒棘球蚴Eg95和Eg.ferritin基因,采用基因拼接法将Eg95和Eg.ferritin融合,将该融合基因Eg95-Eg.ferritin插入到p Fast Bac DUAL载体中,构建重组转座载体后转化DH10Bac感受态细胞,获得重组Bacmid质粒后转染Sf-9昆虫细胞,传毒3代,对表达蛋白进行Western blot鉴定。结果成功克隆了Eg95和Eg.ferritin基因,通过柔性氨基酸linker成功获得了融合基因Eg95-Eg.ferritin,经PCR和酶切鉴定成功构建了重组质粒p Fast Bac DUAL-Eg95-Eg.ferritin,Western blot结果证实表达蛋白能够被包虫病人标准阳性血清识别。结论在Bac-to-Bac杆状病毒表达系统中成功表达了细粒棘球蚴Eg95-Eg.ferritin融合蛋白,与包虫病人标准阳性血清具有良好的反应性。 展开更多
关键词 细粒棘球蚴 Eg95-Eg.ferritin融合基因 杆状病毒 Sf-9细胞 表达
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血清中PD-1、TPS与Ferritin水平联合检测在非小胞肺癌诊断中的价值 被引量:3
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作者 姚力偀 唐友东 《标记免疫分析与临床》 CAS 2019年第4期662-666,共5页
目的探讨血清中PD-1、TPS与Ferritin水平联合检测在非小胞肺癌诊断中的价值。方法 2016年1月至2018年1月期间,我院共收治70例非小细胞肺癌患者(非小细胞肺癌组),并以此作为研究对象。同时以此期间在我院行健康体检正常人群(n=70)作为对... 目的探讨血清中PD-1、TPS与Ferritin水平联合检测在非小胞肺癌诊断中的价值。方法 2016年1月至2018年1月期间,我院共收治70例非小细胞肺癌患者(非小细胞肺癌组),并以此作为研究对象。同时以此期间在我院行健康体检正常人群(n=70)作为对照人群(正常组)。比较上述二组受试者血清PD-1、TPS与Ferritin水平,并对单独或联合检测非小细胞肺癌患者进行统计学分析。结果①非小细胞肺癌患者血清PD-1、TPS与Ferritin水平明显高于正常组(P<0.05)。②非小细胞肺癌组患者组内进行比较,该组患者治疗后血清PD-1、TPS与Ferritin水平较治疗前明显降低(P<0.05)。③PD-1、TPS与Ferritin均与非小细胞肺癌的发展有关,是非小细胞肺癌的独立危险因素。④与单独检测比较,联合检测PD-1、TPS与Ferritin的灵敏度、特异性、准确度最高(P <0.05),且此时ROC曲线面积最大。结论联合检测PD-1、TPS与Ferritin能明显提高非小细胞肺癌诊断的准确度与灵敏度,并增强其诊断特异性,具有一定的临床应用价值。 展开更多
关键词 PD-1 TPS ferritin 非小细胞肺癌
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