Background Anterior chamber associated immune deviation （ACAID） is characterized by a Th2 cell response. GATA-3 has been shown to be necessary for the activation of Th2 cells. This study was designed to examine the ...Background Anterior chamber associated immune deviation （ACAID） is characterized by a Th2 cell response. GATA-3 has been shown to be necessary for the activation of Th2 cells. This study was designed to examine the expression of GATA-3 in the development of ACAID.Methods ACAID was induced by injection of 50 μg interphotoreceptor retinoid binding protein （IRBP） into the anterior chamber （AC） of Wistar rats. Delayed-type hypersensitivity （DTH） was evaluated on day 3, 7, 14, 21, 28 after IRBP inoculation. GATA-3 expression was detected using immunohistochemical staining. The expression of GATA-3 mRNA at different time points after AC injection of IRBP was assayed by reverse transcriptase polymerase chain reaction （RT-PCR）.Results A significant DTH reaction was observed in Wistar rats on day 3 and 5 after II^BP inoculation. Jne DTH reaction was decreased 7 days after IRBP inoculation. GATA-3 expression was weak at both mRNA and protein levels in the normal spleen, but was significantly increased on day 5, 7, 14, and 21 after AC injection of IRBP.Conclusion The expression of GATA-3 is increased during ACAID, suggesting that GATA-3 may be involved in the development of ACAID.展开更多
T lymphocytes with dedicated suppressor function(Treg)play a crucial role in the homeostatic control of immunity in the periphery.Several Treg phenotypes have now been identified in the CD4 and CD8 T cell populations,...T lymphocytes with dedicated suppressor function(Treg)play a crucial role in the homeostatic control of immunity in the periphery.Several Treg phenotypes have now been identified in the CD4 and CD8 T cell populations, suggesting their down-regulatory function in both human and animal models of autoimmunity,transplantation and tumor immunity.Here we will focus on the CD8 Treg population and their ability to specifically inhibit a pathogenic autoimmune response.This review will detail the current advances in the knowledge of CD8 Treg in the context of antigen specificity,phenotype,MHC restriction,mechanism of action,and priming.Cellular & Molecular Immunology.2005;2(1):11-19.展开更多
A symmetrical heterodyne grating interferometer with both a short period and high signal-to-noise ratio is proposed. It possesses good immunity to environmental disturbances and can simultaneously achieve high resolut...A symmetrical heterodyne grating interferometer with both a short period and high signal-to-noise ratio is proposed. It possesses good immunity to environmental disturbances and can simultaneously achieve high resolution and stability. The experimental results show that the standard deviation of 24.67 nm can be realized for the long range of 10 mm. The measurement resolution of better than 2 nm is achieved with the theoretical resolution of 12 pm. Additionally, system stability at less than 1.5 nm is obtained in just over 10 min. The measurement errors, including cosine error, nonlinear error and non-common path error, are discussed as well.展开更多
文摘Background Anterior chamber associated immune deviation （ACAID） is characterized by a Th2 cell response. GATA-3 has been shown to be necessary for the activation of Th2 cells. This study was designed to examine the expression of GATA-3 in the development of ACAID.Methods ACAID was induced by injection of 50 μg interphotoreceptor retinoid binding protein （IRBP） into the anterior chamber （AC） of Wistar rats. Delayed-type hypersensitivity （DTH） was evaluated on day 3, 7, 14, 21, 28 after IRBP inoculation. GATA-3 expression was detected using immunohistochemical staining. The expression of GATA-3 mRNA at different time points after AC injection of IRBP was assayed by reverse transcriptase polymerase chain reaction （RT-PCR）.Results A significant DTH reaction was observed in Wistar rats on day 3 and 5 after II^BP inoculation. Jne DTH reaction was decreased 7 days after IRBP inoculation. GATA-3 expression was weak at both mRNA and protein levels in the normal spleen, but was significantly increased on day 5, 7, 14, and 21 after AC injection of IRBP.Conclusion The expression of GATA-3 is increased during ACAID, suggesting that GATA-3 may be involved in the development of ACAID.
文摘T lymphocytes with dedicated suppressor function(Treg)play a crucial role in the homeostatic control of immunity in the periphery.Several Treg phenotypes have now been identified in the CD4 and CD8 T cell populations, suggesting their down-regulatory function in both human and animal models of autoimmunity,transplantation and tumor immunity.Here we will focus on the CD8 Treg population and their ability to specifically inhibit a pathogenic autoimmune response.This review will detail the current advances in the knowledge of CD8 Treg in the context of antigen specificity,phenotype,MHC restriction,mechanism of action,and priming.Cellular & Molecular Immunology.2005;2(1):11-19.
基金supported by the National Natural Science Foundation of China under Grant No.51275523
文摘A symmetrical heterodyne grating interferometer with both a short period and high signal-to-noise ratio is proposed. It possesses good immunity to environmental disturbances and can simultaneously achieve high resolution and stability. The experimental results show that the standard deviation of 24.67 nm can be realized for the long range of 10 mm. The measurement resolution of better than 2 nm is achieved with the theoretical resolution of 12 pm. Additionally, system stability at less than 1.5 nm is obtained in just over 10 min. The measurement errors, including cosine error, nonlinear error and non-common path error, are discussed as well.
