期刊文献+
共找到5篇文章
< 1 >
每页显示 20 50 100
Isolation and Culture of Rabbit Marrow-derived Mesenchymal Stem Cells 被引量:1
1
作者 Ai-Ming ZHANG Lin CAI(Department of Orthopedics, Zhongnan Hospital of Wuhan University, Wuhan 430071,China) 《生物医学工程学杂志》 EI CAS CSCD 北大核心 2005年第S1期163-165,共3页
关键词 MSCS bone cell isolation and culture of Rabbit Marrow-derived Mesenchymal Stem Cells
下载PDF
Isolation and Culture of a kind of Derived Cells from Differentiation Group Of Murine ESC-like Cell and Their Traits in Culture
2
作者 Qi-Shuang GAO Ting YU Bang-You LI Yan-Yun HAN Chun-Han AIChun-Fang WANG Jing-Fu LV Jian-Ying WU(Wuhan Institute of Pasturage and Veterinary,Wuhan 430000,China) 《生物医学工程学杂志》 EI CAS CSCD 北大核心 2005年第S1期177-178,共2页
关键词 ESC isolation and culture of a kind of Derived Cells from Differentiation Group Of Murine ESC-like Cell and Their Traits in culture
下载PDF
Isolation and differentiation of embryonic stem cells from BALB/c mouse 被引量:1
3
作者 Wei GONG Zhuo-Jing LUO Hua HAN Hong-Yan QIN You-Biao CHU Xue-Yu HU Li-Feng LAN 《Neuroscience Bulletin》 SCIE CAS CSCD 2006年第1期7-13,共7页
Objective To invest the efficient method which can culture and induce embryonic stem cells to neuroeyte in vitro. Methods Isolate the blastula o f 3.5 d from BALB/c species mouse. Culture the cells from inner cell ma... Objective To invest the efficient method which can culture and induce embryonic stem cells to neuroeyte in vitro. Methods Isolate the blastula o f 3.5 d from BALB/c species mouse. Culture the cells from inner cell mass (inner cell mass, ICM) which were isolated by mechanical method on the mouse embryonic fibroblaste cell (MEF) feeder layer or 0.1% gelatin coated dishes. The stem ceils were identified by characterized morphology, alkaline phosphatase stain, differential potency in vivo and immunoehemistry stain. The isolated cells were differentiated by serial induction method that mimicking the intrinsic developmental process of the neural system. Results The isolated cells were positive for alkaline phosphatatse and SSEA-1 ( stage specific embryonic antigen 1 ). Moreover they were identified pluripotent by differentiation in vivo. Therefore the isolated ceils presented the characters of ESCs. Then the isolated cells were able to differentiate into neuroeytes in vitro. Conclusion Mouse embryonic stem ceils isolation, culture and differentiation system has been established. 展开更多
关键词 isolation and culture BALB/c mouse embryonic stem cells DIFFERENTIATION neurocyte
下载PDF
Stem cell properties and neural differentiation of sheep amniotic epithelial cells
4
作者 Xuemin Zhu Xiumei Wang +7 位作者 Guifang Cao Fengjun Liu Yinfeng Yang Xiaonan Li Yuling Zhang Yan Mi Junping Liu Lingli Zhang 《Neural Regeneration Research》 SCIE CAS CSCD 2013年第13期1210-1219,共10页
This study was designed to verify the stem cell properties of sheep amniotic epithelial cells and their capacity for neural differentiation. Immunofluorescence microscopy and reverse transcription-PCR revealed that th... This study was designed to verify the stem cell properties of sheep amniotic epithelial cells and their capacity for neural differentiation. Immunofluorescence microscopy and reverse transcription-PCR revealed that the sheep amniotic epithelial cells were positive for the embryonic stem cell marker proteins SSEA-1, SSEA-3, SSEA-4, TRA-1-60 and TRA-1-81, and the totipotency-associated genes Oct-4, Sox-2 and Rex-1, but negative for Nanog. Amniotic epithelial cells expressed β-Ⅲ-tubulin, glial fibrillary acidic protein, nestin and microtubule-associated protein-2 at 28 days after induction with serum-free neurobasal-A medium containing B-27. Thus, sheep amniotic epithelial cells could differentiate into neurons expressing β-Ⅲ-tubulin and microtubule-associated protein-2, and glial-like cells expressing glial fibrillary acidic protein, under specific conditions. 展开更多
关键词 neural regeneration stem cells SHEEP amniotic epithelial cells isolation and culture stem cecharacteristics DIFFERENTIATION differentiation potential reverse transcription-PCR immunofluorescence microscopy grants-supported paper NEUROREGENERATION
下载PDF
Characterization, isolation, and culture of spermatogonial stem cells in Macaca fascicularis 被引量:1
5
作者 Guo-Ping Mao Ming-Hui Niu +4 位作者 Ying-Hong Cui Rui-Ling Tang Wei Chen Bang Liu Zuping He 《Asian Journal of Andrology》 SCIE CAS CSCD 2021年第3期240-248,共9页
Spermatogonial stem cells(SSCs)have great applications in both reproductive and regenerative medicine.Primates including monkeys are very similar to humans with regard to physiology and pathology.Nevertheless,little i... Spermatogonial stem cells(SSCs)have great applications in both reproductive and regenerative medicine.Primates including monkeys are very similar to humans with regard to physiology and pathology.Nevertheless,little is known about the isolation,the characteristics,and the culture of primate SSCs.This study was designed to identify,isolate,and culture monkey SSCs.Immunocytochemistry was used to identify markers for monkey SSCs.Glial cell line-derived neurotrophic factor family receptor alpha-1(GFRAl)-enriched spermatogonia were isolated from monkeys,namely Macaca fascicularis(M.fascicularis),by two-step enzymatic digestion and magnetic-activated cell sorting,and they were cultured on precoated plates in the conditioned medium.Reverse transcription-polymerase chain reaction(RT-PCR),immunocytochemistry,and RNA sequencing were used to compare phenotype and transcriptomes in GFRAl-enriched spermatogonia between 0 day and 14 days of culture,and xenotransplantation was performed to evaluate the function of GFRAl-enriched spermatogonia.SSCs shared some phenotypes with rodent and human SSCs.GFRAl-enriched spermatogonia with high purity and viability were isolated from M.fascicularis testes.The freshly isolated cells expressed numerous markers for rodent SSCs,and they were cultured for 14 days.The expression of numerous SSC markers was maintained during the cultivation of GFRAl-enriched spermatogonia.RNA sequencing reflected a 97.3%similarity in global gene profiles between 0 day and 14 days of culture.The xenotransplantation assay indicated that the GFRAl-enriched spermatogonia formed colonies and proliferated in vivo in the recipient c-Kitw/w(W)mutant mice.Collectively,GFRAl-enriched spermatogonia are monkey SSCs phenotypically both in vitro and in vivo.This study suggests that monkey might provide an alternative to human SSCs for basic research and application in human diseases. 展开更多
关键词 CHARACTERIZATION isolation and culture Macaca fascicularis spermatogonial stem cells transplantation and transcriptomes
原文传递
上一页 1 下一页 到第
使用帮助 返回顶部