[Objective] The study was to provide the theoretical guidance for the control of rice sheath blight using the mixture of endophytic Bacillus megaterium strain B196 and fungicide. [Method] The toxicity of the mixture o...[Objective] The study was to provide the theoretical guidance for the control of rice sheath blight using the mixture of endophytic Bacillus megaterium strain B196 and fungicide. [Method] The toxicity of the mixture of endophytic strain B196 and jinggangmycin against Rhizoctonia solani was determined by inhibition rate method,and the effect of the mixture on R. solani was also tested. [Result] The mixture of B196 strain and 5% Jinggangmycin water agent with the mass ratio of 1∶68.47 had synergy effect,co-toxicity coefficient （CTC） was 209,the control effect of 200 μg/ml mixture against R. solani was 75.57% in field,which was 17.68% higher than that of single endogenous bacillus strain B196 treatments. [Conclusion] The mixture of endophytic strain B196 and Jinggangmycin had good control effect against R. solani.展开更多
A simple, fast and reliable method was developed for the analysis of jinggangmycin A （validamycin A） in commercial formulations. The running buffer used was acetate buffer （100 mmol/L, pH 4.7） with 15 kV as the ap...A simple, fast and reliable method was developed for the analysis of jinggangmycin A （validamycin A） in commercial formulations. The running buffer used was acetate buffer （100 mmol/L, pH 4.7） with 15 kV as the applied voltage. The detection was achieved by using direct UV mode at 200 nm and the detection limit was 0.2 μg/mL. Linearity in the concentration range of 5-500 μg/mL was excellent （RE 〉 0.999）. The run-to-run repeatability （n = 3）, as expressed by the relative standard deviation （RSD） for migration times and peak areas were less than 0.5% and 3.0% respectively. The mean recovery ranged from 97.2% to 101.4%.展开更多
基金Supported by Guangxi Natural Science Foundation(GSN0728012)Graduate Education Innovation Project in Guangxi University(2008105930904M023)~~
文摘[Objective] The study was to provide the theoretical guidance for the control of rice sheath blight using the mixture of endophytic Bacillus megaterium strain B196 and fungicide. [Method] The toxicity of the mixture of endophytic strain B196 and jinggangmycin against Rhizoctonia solani was determined by inhibition rate method,and the effect of the mixture on R. solani was also tested. [Result] The mixture of B196 strain and 5% Jinggangmycin water agent with the mass ratio of 1∶68.47 had synergy effect,co-toxicity coefficient （CTC） was 209,the control effect of 200 μg/ml mixture against R. solani was 75.57% in field,which was 17.68% higher than that of single endogenous bacillus strain B196 treatments. [Conclusion] The mixture of endophytic strain B196 and Jinggangmycin had good control effect against R. solani.
文摘A simple, fast and reliable method was developed for the analysis of jinggangmycin A （validamycin A） in commercial formulations. The running buffer used was acetate buffer （100 mmol/L, pH 4.7） with 15 kV as the applied voltage. The detection was achieved by using direct UV mode at 200 nm and the detection limit was 0.2 μg/mL. Linearity in the concentration range of 5-500 μg/mL was excellent （RE 〉 0.999）. The run-to-run repeatability （n = 3）, as expressed by the relative standard deviation （RSD） for migration times and peak areas were less than 0.5% and 3.0% respectively. The mean recovery ranged from 97.2% to 101.4%.
