Objective: We aimed to observe the effects of loganin(Log) on serum glycolipid levels and probe the mechanisms focusing on intestinal flora and AMP-activated protein kinase(AMPK) signaling in obese mice.Methods: A hig...Objective: We aimed to observe the effects of loganin(Log) on serum glycolipid levels and probe the mechanisms focusing on intestinal flora and AMP-activated protein kinase(AMPK) signaling in obese mice.Methods: A high-fat diet was given for 12 consecutive weeks to generate the obesity model in institute of cancer research(ICR) mice. Body weight was measured weekly and fasting blood glucose(FBG) was determined every 2 weeks. Both the oral glucose tolerance test and the intraperitoneal insulin tolerance test were performed. The serum levels of total cholesterol(TC), triglyceride, high-density lipoproteincholesterol, low-density lipoprotein-cholesterol(LDL-C), and free fatty acids(FFA) were measured. The expression of key proteins in the AMPK signaling pathway in skeletal muscle tissue was detected by immunoblotting, and gut microbiota were characterized using 16S rDNA sequencing.Results: Log significantly decreased the body weight and the FBG in obese mice(P <.05), and it could restore FBG to normal levels. The total cholesterol, LDL-C, and FFA levels were significantly reduced by Log compared with the obese controls(TC: P =.0020;LDL-C: P =.0233;FFA: P =.0127), and the glucose tolerance of animals was significantly improved(P =.0477). The western blot results showed that Log could upregulate the protein expression of Adenosine 5‘-monophosphate(AMP)-activated protein kinase(AMPKa), Sirtuin 1(SIRT1), and peroxisome proliferator-activated receptor-gamma coactivator-1 alpha(PGC1a) in skeletal muscle tissue of obese mice. 16S rDNA sequencing indicated that Log reduced the diversity of the gut flora in feces and altered the floral composition of obese mice.Conclusions: Log was effective in reducing body weight and improving glucolipid metabolism in obese mice, probably through activating AMPK signaling and regulating intestinal microbial diversity.展开更多
目的优化加味缩泉合剂制备工艺,确定其质量标准。方法以出膏率、莫诺苷和马钱苷总量为指标,通过正交试验确定加味缩泉合剂的制备工艺。采用Diamonsil Plus 5μm C_(18)(250mm×4.6mm)色谱柱;流动相A为乙腈,流动相B为0.3%磷酸溶液,...目的优化加味缩泉合剂制备工艺,确定其质量标准。方法以出膏率、莫诺苷和马钱苷总量为指标,通过正交试验确定加味缩泉合剂的制备工艺。采用Diamonsil Plus 5μm C_(18)(250mm×4.6mm)色谱柱;流动相A为乙腈,流动相B为0.3%磷酸溶液,进行梯度洗脱;检测波长为240nm;流速为1.0ml/min;柱温为35℃。结果确定提取工艺的条件为提取2次,分别以10倍量水、8倍量水,提取1.5h、1.0h。建立的莫诺苷、马钱苷含量测定方法的专属性、重现性、准确度均较好;莫诺苷在进样量0.1962~0.9810μg范围内线性关系良好,平均加样回收率为100.83%、相对标准偏差(relative standard deviation,RSD)为1.26%,精密度、稳定性等均符合要求。马钱苷在进样量0.0995~0.7960μg范围内线性关系良好,平均加样回收率为97.10%、RSD为0.84%,精密度、稳定性等均符合要求。结论最终确定的加味缩泉合剂制备工艺简单,操作易行。含量测定方法专属性强、准确性可靠,可用于加味缩泉合剂的质量控制。展开更多
Loganin(LOG),a bioactive compound derived from Cornus officinalis Siebold & Zucc,has been understudied in the context of osteoarthritis(OA)treatment.In this study,we induced an inflammatory response in chondrocyte...Loganin(LOG),a bioactive compound derived from Cornus officinalis Siebold & Zucc,has been understudied in the context of osteoarthritis(OA)treatment.In this study,we induced an inflammatory response in chondrocytes using lipopolysaccharide(LPS)and subsequently treated these cells with LOG.We employed fluorescence analysis to quantify reactive oxygen species(ROS)levels and measured the expression of NLRP3 and nuclear factor erythropoietin-2-related factor 2(NRF2)using real-time quantitative polymerase chain reaction(qRT-PCR),Western blotting,and immunofluorescence(IF)techniques.Additionally,we developed an OA mouse model by performing medial meniscus destabilization(DMM)surgery and monitored disease progression through micro-com-puted tomography(micro-CT),hematoxylin and eosin(H&E)staining,safranin O and fast green(S&F)staining,and immunohisto-chemical(IHC)analysis.Our results indicate that LOG significantly reduced LPS-induced ROS levels in chondrocytes,inhibited the activation of the NLRP3 inflammasome,and enhanced NRF2/heme oxygenase 1(HO-1)signaling.In vivo,LOG treatment mitigated cartilage degradation and osteophyte formation triggered by DMM surgery,decreased NLRP3 expression,and increased NRF2 expres-sion.These findings suggest that LOG has a protective effect against OA,potentially delaying disease progression by inhibiting the ROS-NLRP3-IL-1β axis and activating the NRF2/HO-1 pathway.展开更多
基金supported by the Qi Huang Scholars Program of the State Administration of Traditional Chinese Medicine(10400633210005)the National Natural Science Foundation of China (NSFC82174329&NSFC81503540)+1 种基金the Key Drug Development Program (2012ZX09103201-005)the Key Research Project of Beijing University of Chinese Medicine (2020-JYB-ZDGG-029)。
文摘Objective: We aimed to observe the effects of loganin(Log) on serum glycolipid levels and probe the mechanisms focusing on intestinal flora and AMP-activated protein kinase(AMPK) signaling in obese mice.Methods: A high-fat diet was given for 12 consecutive weeks to generate the obesity model in institute of cancer research(ICR) mice. Body weight was measured weekly and fasting blood glucose(FBG) was determined every 2 weeks. Both the oral glucose tolerance test and the intraperitoneal insulin tolerance test were performed. The serum levels of total cholesterol(TC), triglyceride, high-density lipoproteincholesterol, low-density lipoprotein-cholesterol(LDL-C), and free fatty acids(FFA) were measured. The expression of key proteins in the AMPK signaling pathway in skeletal muscle tissue was detected by immunoblotting, and gut microbiota were characterized using 16S rDNA sequencing.Results: Log significantly decreased the body weight and the FBG in obese mice(P <.05), and it could restore FBG to normal levels. The total cholesterol, LDL-C, and FFA levels were significantly reduced by Log compared with the obese controls(TC: P =.0020;LDL-C: P =.0233;FFA: P =.0127), and the glucose tolerance of animals was significantly improved(P =.0477). The western blot results showed that Log could upregulate the protein expression of Adenosine 5‘-monophosphate(AMP)-activated protein kinase(AMPKa), Sirtuin 1(SIRT1), and peroxisome proliferator-activated receptor-gamma coactivator-1 alpha(PGC1a) in skeletal muscle tissue of obese mice. 16S rDNA sequencing indicated that Log reduced the diversity of the gut flora in feces and altered the floral composition of obese mice.Conclusions: Log was effective in reducing body weight and improving glucolipid metabolism in obese mice, probably through activating AMPK signaling and regulating intestinal microbial diversity.
文摘目的优化加味缩泉合剂制备工艺,确定其质量标准。方法以出膏率、莫诺苷和马钱苷总量为指标,通过正交试验确定加味缩泉合剂的制备工艺。采用Diamonsil Plus 5μm C_(18)(250mm×4.6mm)色谱柱;流动相A为乙腈,流动相B为0.3%磷酸溶液,进行梯度洗脱;检测波长为240nm;流速为1.0ml/min;柱温为35℃。结果确定提取工艺的条件为提取2次,分别以10倍量水、8倍量水,提取1.5h、1.0h。建立的莫诺苷、马钱苷含量测定方法的专属性、重现性、准确度均较好;莫诺苷在进样量0.1962~0.9810μg范围内线性关系良好,平均加样回收率为100.83%、相对标准偏差(relative standard deviation,RSD)为1.26%,精密度、稳定性等均符合要求。马钱苷在进样量0.0995~0.7960μg范围内线性关系良好,平均加样回收率为97.10%、RSD为0.84%,精密度、稳定性等均符合要求。结论最终确定的加味缩泉合剂制备工艺简单,操作易行。含量测定方法专属性强、准确性可靠,可用于加味缩泉合剂的质量控制。
基金supported by the National Natural Science Foundation of China(No.82074462)the Major Research Project of Guangzhou University of Chinese Medicine(No.2021xk53)the First Affiliated Hospital of Guangzhou University of Chinese Medicine National Center for Traditional Chinese Medicine Inheritance and Innovation Special Research(No.2022QN02).
文摘Loganin(LOG),a bioactive compound derived from Cornus officinalis Siebold & Zucc,has been understudied in the context of osteoarthritis(OA)treatment.In this study,we induced an inflammatory response in chondrocytes using lipopolysaccharide(LPS)and subsequently treated these cells with LOG.We employed fluorescence analysis to quantify reactive oxygen species(ROS)levels and measured the expression of NLRP3 and nuclear factor erythropoietin-2-related factor 2(NRF2)using real-time quantitative polymerase chain reaction(qRT-PCR),Western blotting,and immunofluorescence(IF)techniques.Additionally,we developed an OA mouse model by performing medial meniscus destabilization(DMM)surgery and monitored disease progression through micro-com-puted tomography(micro-CT),hematoxylin and eosin(H&E)staining,safranin O and fast green(S&F)staining,and immunohisto-chemical(IHC)analysis.Our results indicate that LOG significantly reduced LPS-induced ROS levels in chondrocytes,inhibited the activation of the NLRP3 inflammasome,and enhanced NRF2/heme oxygenase 1(HO-1)signaling.In vivo,LOG treatment mitigated cartilage degradation and osteophyte formation triggered by DMM surgery,decreased NLRP3 expression,and increased NRF2 expres-sion.These findings suggest that LOG has a protective effect against OA,potentially delaying disease progression by inhibiting the ROS-NLRP3-IL-1β axis and activating the NRF2/HO-1 pathway.
