The German Mouse Clinic was established as a phenotyping center to provide the scientific community with systematic standardized phenotyping of mouse models from various genetic backgrounds.We found metabolic phenotyp...The German Mouse Clinic was established as a phenotyping center to provide the scientific community with systematic standardized phenotyping of mouse models from various genetic backgrounds.We found metabolic phenotypes in nine out of 20 mutant lines screened in a primary screen.Based on these findings,the mutants were analyzed in secondary and tertiary screens.Mice of a sample mutant line,isolated from the ENU-screen at the National Research Center for Environment and Health in Munich,were found to have lower body weight,consume less food,but have higher ratios of metabolized energy per unit body weight compared with their wild-type littermates.Basal metabolic rate and heat production were simultaneously increased by 16–18%,whereas body fat content was reduced by 11–16%.The combination of various parameters of energy consumption,expenditure and energy storage illustrate the metabolic demands of the sample mutant mouse line and demonstrate the utility of the powerful phenotyping tool used at the German Mouse Clinic.展开更多
Placenta,a temporary organ first formed during the development of a new life is essential for the survival and growth of the fetus in eutherian mammals.It serves as an interface for the exchange of nutrients,gases and...Placenta,a temporary organ first formed during the development of a new life is essential for the survival and growth of the fetus in eutherian mammals.It serves as an interface for the exchange of nutrients,gases and wastes between the maternal and fetal compartments.During the past decades,studies employing gene-engineered mouse mutants have revealed a wide range of signaling molecules governing the trophoblast development and function during placentation under various pathophysiological conditions.Here,we summarize the recent progress with particular respect to the involvement of developmental genes during placentation.展开更多
文摘The German Mouse Clinic was established as a phenotyping center to provide the scientific community with systematic standardized phenotyping of mouse models from various genetic backgrounds.We found metabolic phenotypes in nine out of 20 mutant lines screened in a primary screen.Based on these findings,the mutants were analyzed in secondary and tertiary screens.Mice of a sample mutant line,isolated from the ENU-screen at the National Research Center for Environment and Health in Munich,were found to have lower body weight,consume less food,but have higher ratios of metabolized energy per unit body weight compared with their wild-type littermates.Basal metabolic rate and heat production were simultaneously increased by 16–18%,whereas body fat content was reduced by 11–16%.The combination of various parameters of energy consumption,expenditure and energy storage illustrate the metabolic demands of the sample mutant mouse line and demonstrate the utility of the powerful phenotyping tool used at the German Mouse Clinic.
基金the National Basic Research Program of China(No.2011CB944401)the National Natural Science Foundation of China(Grant Nos.2299390,31000659)+1 种基金the Beijing Natural Science Foundation(No.5091002)Haibin Wang is a recipient of“National Science Foundation for Distinguished Young Scholars”(No.30825015).
文摘Placenta,a temporary organ first formed during the development of a new life is essential for the survival and growth of the fetus in eutherian mammals.It serves as an interface for the exchange of nutrients,gases and wastes between the maternal and fetal compartments.During the past decades,studies employing gene-engineered mouse mutants have revealed a wide range of signaling molecules governing the trophoblast development and function during placentation under various pathophysiological conditions.Here,we summarize the recent progress with particular respect to the involvement of developmental genes during placentation.
