Effect of rice cultivar on greenhouse-gas emissions from rice-fish co-culture

In aquaculture,co-culturing rice with fish may mitigate greenhouse-gas emissions.In this study,co-culture of four rice cultivars in a laboratory-scale rice–fish system reduced CH_(4)and N_(2)O emissions relative to fish monoculture.Differences in CH_(4)and N_(2)O emissions among rice cultivars primarily stem from the differential effects of rice plants on plant-mediated CH_(4)transport,CH_(4)oxidation and nitrogen absorption.

Variation of grain Cd and Zn concentrations of 110 hybrid rice cultivars grown in a low-Cd paddy soil

Enhanced Cd uptake and Zn depletion in rice grains and high potential for food Cd exposure by the high-yielding hybrid cultivars of China had been addressed. A field experiment was conducted in 2006 to determine the difference in grain Cd and Zn between cultivars. Total 110 cultivars including super rice and common hybrid rice cultivars were grown on a single paddy soil （Entic Haplaquept） with a neutral reaction and low total Cd content. Grain Cd and Zn concentrations were determined with graphite atomic adsorption spectrophotometer （GFAAS） and flame atomic adsorption spectrophotometer （AAS） respectively. Wide variation of Cd content in grain was found in a range of 0.004-0.057 mg/kg, while the Zn content in a range of 10.25-30.06 mg/kg among the cultivars. Higher Cd but lower Zn concentration in grains of super rice cultivars was observed compared to the common hybrid ones. A highly significant positive linear correlation of grain Cd/Zn with grain Cd was found for super rice and common hybrid cultivars, meanwhile much higher slope for these hybrid cultivars than the reported non-hybrid cultivars was also observed. Using the limit value of the Chinese chemical guidelines for foods （MOHC and SSC, 2005）, calculated potential risk of food Cd exposure with ＂Zn hungry＂ through diet intake was prominent with all the studied 110 hybrid rice cultivars, possessing high potential health problems for rice production in South China using the super rice cultivars. Breeding of genotypes of rice cultivars with low grain Cd and low Cd/Zn ratio is needed for rice production in acidic red soils where Cd bioavailability is prevalently high.

Variation of Cd concentration in various rice cultivars and derivation of cadmium toxicity thresholds for paddy soil by species-sensitivity distribution

It is imperative to derive an appropriate cadmium (Cd) health risk toxicity threshold for paddy soils to ensure the Cd con-centration of rice grains meet the food safety standard. In this study, 20 rice cultivars from the main rice producing areas in China were selected, and a pot-experiment was conducted to investigate transformation of Cd in paddy soil-rice system with 0 (CK), 0.3 mg kg–1 (T1) and 0.6 mg kg–1 (T2) Cd treatments in greenhouse. The results showed that Cd concentrations of rice grains existed signiifcant difference (P<0.05) in 20 rice cultivars under the same Cd level in soil. The Cd concentrations of rice grains of the CK, T1 and T2 treatments were in the range of 0.143–0.202, 0.128–0.458 and 0.332–0.806 mg kg–1, respectively. Marked differences of the ratios of Cd concentration for soil to rice grain (BCFs) and transfer factors (TFs, root to grain and straw to grain) among the tested cultivars were observed in this study. The bioconcentration factors (BCFgrain) and TFs of the 20 rice cultivars were 0.300–1.112 and 0.342–0.817, respectively. The TFs of Cd from straw to grain ranged from 0.366 to 1.71, with signiifcant differences among these 20 rice cultivars. The bioconcentration factors (BCFgrain) and TFs among the 20 rice cultivars ranged from 0.300–1.112 and 0.342–0.817, respectively. The species-sensitivity distribu-tion (SSD) of Cd sensitivity of the rice species could be iftted wel with Burr-III (R2=0.987) based on the data of BCFs. The toxicity threshold of Cd derived from SSD for the paddy soil was 0.507 mg kg–1 in the present study.

Difference of Nitrogen Uptake and Use Efficiency in Conventional Indica Rice Cultivars with Different Nitrogen Use Efficiency for Grain Output

[Objective] The study aimed to confirm difference of nitrogen uptake and used efficiency with different nitrogen use efficiency for grain output (NUEg) types of indica rice.[Method] 88 and 122 conventional indica rice cultivars were solution-cultured in 2001 and 2002, respectively. Dry matter weight (including root system, culm and sheath, leaves, panicle), nitrogen content of different organs, yield and its components were measured. The tested rice cultivars were classified into 6 types (i.e. A, B, C, D, E and F, A was the lowest, and F was the highest) based on their NUEg level by the MinSSw method.[Result](1)Difference of NUEg of the cultivars used in this study were very large; (2) No significant difference of N content at heading stage was observed among different NUEg types of indica rice. In the cultivars with higher NUEg, however, N content in leaf, stem-sheath and entire rice plant were lower at mature stage. (3)Cultivars with higher NUEg were characterized with lower N uptake before heading and at mature stage; (4) Cultivars with higher NUEg were characterized with higher N use efficiency in biomass production and harvest index. [Conclusion] The cultivars with higher NUEg showed lower N uptake and N content, while nitrogen use efficiency was higher.

Difference in Selenium Accumulation in Shoots of Two Rice Cultivars

Two japonica rice （Oryza sativa L.） cultivars, Xiushui 48 and S. Andrea, differing in their ability to accumulate Se in the grain （as high as a three-fold difference）, were compared for selenium （Se） accumulation in their shoots when their growth media was supplied with different forms of Se. Results indicated that when treated with 0.25μmol L^-1 Na2SeO3, Xiushui 48 accumulation of Se in the shoots was significantly more rapid （P〈0.05） than S. Andrea, probably because of greater Se uptake and transport in Xiushui 48. Xiushui 48 rice seedlings had a higher shoot-Se accumulation rate and absorbed selenocysteine （Se-Cys） more rapidly than S. Andrea seedlings. However, when treated with Se as 0.25μmol L^-1 selenomethionine （Se-Met）, the S. Andrea seedlings＇ accumulation rate was significantly greater （P〈0.05） than that of Xiushui 48. Possibly, the high Se accumulation rate of Xiushui 48 seedling shoots compared to S. Andrea shoots was the result of a higher capacity of Xiushui 48 to transform selenite to organic Se compounds and a higher selenite uptake rate.

N Accumulation and Translocation in Four Japonica Rice Cultivars at Different N Rates

Developing high-yielding rice(Oryza sativa L.)cultivars depends on having a better understanding of nitrogen(N) accumulation and translocation to the ear during the reproductive stage.Field experiments were carried out to evaluate the genetic variation for N accumulation and translocation in different Japonica rice cultivars at different N rates and to identify any relationship to grain yield in southeast China.Four Japonica cultivars with similar agronomic characteristics were grown at two experimental sites in 2004 with three N rates of 0,60,and 180 kg N ha^(-1).Dry weights and N contents of rice plants were measured at tillering,initiation,anthesis,and maturity.Grain yields exhibited significant differences (P<0.05)among the cultivars and N application rates.Increasing N rates improved N uptake at anthesis and maturity in all four cultivars(P<0.05).N translocation from vegetative organs to the grains increased with enhanced N rates (P<0.05).N translocation to the grains ranged from 9 to 64 kg N ha^(-1)and N-translocation efficiency from 33% to 68%. Grain yield was linear to N uptake at anthesis(r^2=0.78^(**))and N translocation(r^2=0.67^(**)).Thus,cultivars with a high N uptake at anthesis,low residual N in the straw at maturity,and appropriate low N fertilizer supply in southeast China should efficiently increase N-recovery rate while maintaining grain yield and soil fertility.

Agronomic performance of drought-resistance rice cultivars grown under alternate wetting and drying irrigation management in southeast China

Compared to drought-susceptible rice cultivars(DSRs),drought-resistance rice cultivars(DRRs)could drastically reduce the amount of irrigation water input and simultaneously result in higher grain yield under water-saving irrigation conditions.However,the mechanisms underlying these properties are unclear.We investigated how improved agronomic traits contribute to higher yield and higher water use efficiency(WUE)in DRRs than in DSRs under alternate wetting and drying(AWD).Two DRRs and two DSRs were field-grown in 2015 and 2016 using two different irrigation regimes:continuous flooding(CF)and AWD.Under CF,no statistical differences in grain yield and WUE were observed between DRRs and DSRs.Irrigation water under the AWD regime was 275–349 mm,an amount 49.8%–56.2% of that(552–620 mm)applied under the CF regime.Compared to CF,AWD significantly decreased grain yield in both DRRs and DSRs,with a more significant reduction in DSRs,and WUE was increased in DRRs,but not in DSRs,by 9.9%–23.0% under AWD.Under AWD,DRRs showed a 20.2%–26.2% increase in grain yield and an 18.6%–24.5% increase in WUE compared to DSRs.Compared to DSRs,DRRs showed less redundant vegetative growth,greater sink capacity,higher grain filling efficiency,larger root biomass,and deeper root distribution under AWD.We conclude that these improved agronomic traits exert positive influences on WUE in DRRs under AWD.

Subcellular Cd accumulation characteristic in root cell wall of rice cultivars with different sensitivities to Cd stress in soil

The variations of grain cadmiun（Cd） concentrations, translocation factors（TFs） of Cd from roots to shoots/grains of six rice cultivars, characterized with different Cd-sensitivities in polluted soil were studied, the selected rice cultivars were Xiangzao 17（R1）, Jiayu 211（R2）, Xiangzao 42（R3）, Zhuliangyou 312（R4）, Zhuliangyou 611（R5）, and Jinyou 463（R6）, respectively. The Cd subcellular distribution and Cd binding characteristics on subcellular fractions of rice root cell wall（CW） were further investigated. The results showed that the rice grain Cd contents varied significantly, with a maximum variation of 47.0% among the cultivars, the largest grain Cd content was observed with cultivar R1（Cd-sensitivity cultivar） and the smallest with R5（Cd-tolerance cultivar）. The translocation factors of Cd from roots to shoots（TF_（shoot）） and roots to grains（TF_（grain）） varied greatly among the cultivars. In general, the TFgrain of the cultivars followed the order of R1〉R2〉R3〉R4〉 R6-R5. The Cd concentration（mg kg^（–1） FW） in the fraction of root CW, the fraction of cell wall removing pectin（CW-P） and the fraction of cell wall removing pectin and hemicellulose（CW-P-HC） of the cultivars generally followed the order of CW-P〉CW〉CWP-HC; the ratios of Cd concentration（mg kg–1 FW） in the fraction of CW-P to that of CW were mostly more than 1.10, while the ratios of Cd concentration in the fraction of CW-P-HC to that of CW were mostly less than 0.60, indicating that Cd was mainly stored in the hemicellulose of the root CW. The ratios of Cd of CW-P-HC to CW generally followed the descending order of R1~R2〉R3〉R4〉R5~R6 for the cultivars, which implied that hemicellulose is probably the main subcellular pool for transferring Cd into rice grain, and it restrains the translocation of Cd from shoot to the grain, especially for the Cd-tolerance cultivars（R5 and R6）, the compartmentation of more Cd in hemicellulose in root CW is probably one of the main mechanisms for Cd tolerance of rice cultivars.

Molecular marker for screening rice cultivars with intermediate amylose content in indica

The waxy(Wx)gene of rice encodes the granule-bound starch synthase(GBSS),which was required in the synthesis of amylose in endosperm.Theamylose content(AC)of rice endosperm played an important role in grain yield,palatability,and processing quality.Our previous researehes showed that en-dosperm AC and GBSS contents were correlated with the ability of excising intron1 from the leader sequence of the Wx transcript.Cultivars with high endosperm

Genetic Diversity and Structure of New Inbred Rice Cultivars in China

A total of 408 inbred rice cultivars bred in the last decade were analyzed for 24 SSR markers. The results showed the genetic diversity of indica cultivars was higher than that of japonica cultivars, and the genetic diversity of new cultivars raised in recent years was lower. Among the six rice cropping regions （RCRs） in China, genetic diversity was the highest in the central rice region （RCR-II） and the southwest rice region （RCR-III）. Genetic differences among subpopulations of japonica were more complex than those in indica. Differentiation among seasonal ecotypes and RCRs in indica populations was unclear, but differentiation between RCR-II and northeast rice region （RCR-V） was more distinct for japonica cultivars. Considering the North rice region （RCR-IV） has very low genetic diversity among the tested cultivars, it is important to broaden the genetic background for future cultivars in rice breeding programs.

Determination of Upland Rice Cultivar Coefficient Specific Parameters for DSSAT (Version 4.7)-CERES-Rice Crop Simulation Model and Evaluation of the Crop Model under Different Temperature Treatments conditions

To develop basis for strategic or arranged decision making towards crop yield improvement in Thailand, a new method in which crop models could be used is essential. Therefore, the objective of this study was to measure cultivar specific parameters by using DSSAT (v4.7) Cropping Simulation Model (CSM) with five upland rice genotypes namely Dawk Pa-yawm, Mai Tahk, Bow Leb Nahng, Dawk Kha 50 and Dawk Kahm. Experiment was laid out in a Completely Randomized Design (CRD) with split plot design. Results showed that five upland rice genotypes had significantly affected each other by different temperature treatments (28°C, 30°C, 32°C) with grain yield, tops weight, harvest index, flowering, and maturity date. At the same time, all the phenological traits had highly significant variation with the genotypes. The cultivar specific parameters obtained by using a temperature tolerant cultivar (Basmati 385) with five upland genotypes involved in the DSSAT4.7-CSM. Model evaluation results indicated that utilizing the estimated cultivar coefficient parameters, model simulated well with varying temperature treatments as indicated by the agreement index (d-statistic) closer to unity. Hence, it was estimated that model calibration and evaluation was realistic in the limits of test cropping seasons and that CSM fitted with cultivar specific parameters can be used in simulation studies for investigation, farm managing or decision making. This electronic document is a “live” template. The various components of your paper [title, text, heads, etc.] are already defined on the style sheet, as illustrated by the portions given in this document.

Evaluation of Drought Tolerant Rice Cultivars Using Drought Tolerant Indices under Water Stress and Irrigated Condition

Drought is the most critical abiotic factor reducing rice yield in rainfed and drought prone areas. Majority of rice cultivated area in south Asia are under rainfed, where water stress at any of the critical growth stage causes sharp decline in yield. Research was conducted in western Nepal at farmers field around the close vicinity of Regional Agriculture Research Center (RARS) Nepalgunj. Ten different rice cultivars were evaluated under drought stress and non-stress condition during the year 2017 to identify superior stress tolerant cultivars. Different drought tolerance indices like stress tolerance (TOL), Stress tolerance index (STI), Stress susceptibility index (SSI), were tested in screening superior rice cultivars. Significant reduction in mean grain yield was observed under drought stress in all rice cultivars under the study. The rice cultivars with high STI values and low TOL and SSI were identified as superior drought tolerant cultivars. Based on results of different tolerance indices, Sukha series of rice, Sukha Dhan 1-6 and Radha-4 showed low TOL and SSI and high STI values and were identified as drought tolerant cultivars. The present study reveals that selection based on drought tolerance indices can be an efficient tool in identification of superior drought tolerant cultivars with higher yield and stability.

Diversity Centers of Rice Cultivar in Yunnan and Their Correlation with Ecological and Cultural Factors

Ecological and cultural factors have strong impacts on the distribution and cultivation of agricultural cultivar., In this paper, the correlation between diversity centers of rice cultivar and ecological, cultural factors was probed, based on datasets of rice cultivar in Yunnan, Southwest China. The results showed that diversity centers of rice cultivar were observed in Southwest Yunnan, South Yunnan and Southeast Yunnan, which may be related to the local culture of rice production and warm, humid climate. For the diversity center in South Yunnan, culture of rice production of Dai and Hani people may play important roles. The diversity center in Southwest Yunnan may relate to the culture of rice production of Dai, Jingpo and Lahu people, and for the diversity center in Southeast Yunnan, Miao, Yao and Zhuang people＇s culture in rice production can not be underestimated. Traditional culture promoted the cultivation of rice cultivars, and high diversity of rice cultivars facilitate the preservation and continuation of the traditional culture as well.

High-efficiency breeding of early-maturing rice cultivars via CRISPR/Cas9-mediated genome editing

Rice is a staple food for more than half of the human population.It has been estimated that by 2030,40%more rice needs to be produced in order to meet the growing demand（Khush,2005）.One of the strategies to improve rice productivity is to enlarge rice growth areas, such as the northward expansion of the growth region in Heilongjiang Province, the northernmost region of China （Li et al., 2015）. However, the northward cultivation is accompanied with daylength extension and temperature decrease, which are unfavor- able for rice, a tropical short-day plant, to complete flowering and seed setting. Thus, the need for early-maturing rice cultivars with extremely low photoperiod sensitivity is urgent.

Evaluation of the occluded carbon within husk phytoliths of 35 rice cultivars

Rice is a well-known silicon accumulator. During its periods of growth, a great number of phytoliths are formed by taking up silica via the plant roots. Concurrently, carbon in those phytoliths is sequestrated by a mechanism of long-term biogeochemical processes within the plant. Phytolith occluded C （PhytOC） is very stable and can be retained in soil for longer than a millennium. In this study, we evaluated the carbon bio- sequestration within the phytoliths produced in rice seed husks of 35 rice cultivars, with the goal of finding rice cultivars with relatively higher phytolith carbon sequestra- tion efficiencies. The results showed that the phytolith contents ranged from 71.6 mg. g^-1 to 150.1 mg. g^-1, and the PhytOC contents ranged from 6.4 mg.g^-1 to 38.4 mg.g^-1, suggesting that there was no direct correlation between the PhytOC content and the content of rice seed husk phytoliths （R = 0.092, p 〉 0.05）. Of all rice cultivars, six showed a higher carbon sequestration efficiency in phytolith seed husks. Additionally, the carbon bio- sequestration within the rice seed husk phytoliths was approximately 0.45-3.46 kg-e-CO2-ha^-1. yr^-1. These rates indicate that rice cultivars are a potential source of carbon biosequestration which could contribute to the global carbon cycle and climate change.

Screening of Drought Resistance Identification Indexes for Rice(Oryza sativa L.) Cultivars in Ningxia

The morphological characters, physiological characters, yield traits and yield per plant of total 49 rice cultivars from Ningxia were investigated under conditions of water stress and non-water stress so as to determine the relationship between each trait and yield per plant under water stress and the relationship between each relative character and drought resistance coefficient under water stress and non-water stress. The correlation, grey correlation, stepwise regression and path analyses showed that the tiller number per plant, plant height, grain density, effective panicle number per plant and grain number per panicle, total 5 traits, were significantly correlated with the drought resistance of rice, and they could be used to identify the drought resistance of rice in Ningxia. In addition, the drought resistance of rice was graded qualitatively according to the subordinate function value of corresponding drought resistance coefficient. The results showed that among the 49 rice cultivars from Ningxia,6 rice cultivars were highly drought resistant, and 9 rice cultivars were moderately drought resistant, suggesting that the evaluation method was feasible and effective.

Sources of Nitrogen in Combination with Systems of Irrigation Influence the Productivity of Modern Rice(Oryza sativa L.)Cultivars during Dry Season in Sub-Tropical Environment

In irrigated agricultural systems,nitrogen(N)and water are the vital resources for sustainability of the crop production in the modern era of climate change.The current study aimed to assess the impact of water and N management on the productivity of irrigated rice cultivars.In the context,a field observation was done at the research farm of Bangladesh Agricultural University,Mymensingh,during dry seasons in consecutive two years(2018–2019 and 2019–2020).The experiments were set up following split-plot design assigning water management in the main plots,nitrogen management in the sub-plots,and the cultivars were approved in the split-split plot with three replications.After two years observation,it was revealed that rice cultivar Binadhan-8 gave the maximum value of leaf area index,number effective tillers hill-1 and grains panicle-1 which lead to the higher grain yield(GY).Substantial relationships were observed among the concentration of N,growth,total dry matter(TDM)and N content,N uptake,N utilization effectiveness,and GY.However,with little exception,the Combined effect of water and N,cultivars and water management were varied significantly for all parameters.Finally,the results of the current study concluded that application of irrigation at 8 days after the disappearance of ponded water and source of 105 kg N ha-1 from PU+Poultry manure are the best management approach for the excellent performance of rice cultivar Binadhan-8.

Effects of elevated [CO_2] on stem and root lodging among rice cultivars

Studies showed that elevated [CO2] would improve photosynthetic rates and enhance yields of rice;however,few studies have focused on the response of rice lodging,which is a major cause of cereal yield loss and quality reduction,under elevated [CO2].In this study,we examined the effects of elevated [CO2] on stem and root lodging using 4 rice cultivars(86Y8,japonica hybrid;LYP9,2-line indica hybrid;variety 9311,type of indica inbred rice,and SY63,3-line indica hybrid) grown under two [CO2] levels:400 and 680 μmol mol-1.Our results indicated that under elevated [CO2],the stem-lodging risk(SLR) of 9311 decreased,while in SY63 the SLR increased,86Y8 and LYP9 were not significantly affected;the risk of root lodging was reduced for all cultivars,because root biomass(instead of root number) and bending strength were increased significantly,and then the increase of anti-lodging ability is far higher than that of self-weight mass moment for all cultivars.These findings suggested that higher [CO2] can enhance the risk of stem-lodging for cultivars with strong-[CO2]-responses,but may not aggravate the root lodging for all rice cultivars.

Genetic diversity of rice cultivars (Oryza sativa L.) in China and the temporal trends in recent fifty years

To understand geographical distribution of the genetic diversity of rice cultivars {Oryza sativa L.) and its trends in recent fifty years in China, 453 ’ accessions were analyzed by 36 microsatellites loci and 42 phenotypic traits. Results revealed that the genetic diversity by SSRs is highly consistent with that by phenotypic traits and the genetic diversity of indica cultivars was higher than that of japonica cultivars; the genetic diversity of cultivars declined from 1950s to 1980s and then increased greatly; among the six rice ecological zones (REZs), genetic diversity of REZTl was the highest and those of REZV and REZVT were the lowest at both DNA and phenotypic level. Jiangsu and Jiangxi provinces in the middle and lower reaches of Yangtze River and Sichuan province in southwest of China were the areas with the highest genetic diversity. Breeders in REZV which is an important japonica rice area but with very low genetic diversity should explore more gene resources to widen the genetic backgrounds of cultivars.

Effect of Nutrient Management and Planting Geometry on Productivity of Hybrid Rice (<i>Oryza sativa</i>L.) Cultivars

Field experiments were conducted during the wet seasons of 2006 and 2007 at the Agricultural Experimental Farm of the Indian Statistical Institute, Giridih, a part of eastern plateau region of India. The study was designed to investigate the effect of planting geometry and nutrient management practices on productivity of two hybrid rice cultivars. Split-plot design with three replications was adopted to carry out the experiment by allocating combinations of treatments of planting geometry and rice cultivar in main-plots and nutrient management treatments in sub-plots. “CNRH-3” rice proved its efficiency in terms of grain yield that was also reflected in yield attributing characters such as number of productive tillers, number of grains per panicle, length of panicle, panicle weight, test weight and harvest index. Higher rice grain yield was registered when the cultivars grown in 20 cm × 20 cm planting geometry. Rice cultivars grown with the application of inorganic fertilizers alone produced maximum grain yield and also recorded higher values of ancillary characters. The maximum amount of N, P and K was taken up by the “CNRH-3” rice, whereas maximum residual soil fertility was recorded in “Pro Agro 6201” rice. Maximum N, P and K uptake values were recorded in 20 cm × 20 cm crop geometry and inorganic fertilizers treatment.