An Improvement of the Mass Flux Convection Parameterization Scheme and its Sensitivity Tests for Seasonal Prediction over China

A modified cumulus parameterization scheme, suitable for use in a seasonal forecast model, is presented. This parameterization scheme is an improvement of the mass flux convection scheme developed by Gregory and Rowntree (1989; 1990). This convection scheme uses a 'bulk' cloud model to present an ensemble of convective clouds, and aims to represent shallow, deep, and mid-level convection. At present, this convection scheme is employed in the NCC T63L20 model (National Climate Center, China Meteorological Administration). Simulation results with this scheme have revealed some deficiencies in the scheme, although to some extent, it improves the accuracy of the simulation. In order to alleviate the deficiencies and reflect the effect of cumulus convection in the actual atmosphere, the scheme is modified and improved. The improvements include (i) the full estimation of the effects of the large-scale convergence in the lower layer upon cumulus convection, (ii) the revision of the initial convective mass flux, and (iii) the regulation of convective-scale downdrafts. A comparison of the results obtained by using the original model and the modified one shows that the improvement and modification of the original convection scheme is successful in simulating the precipitation and general circulation field, because the modified scheme provides a good simulation of the main features of seasonal precipitation in China, and an analysis of the anomaly correlation coefficient between the simulation and the observations confirms the improved results.

Application of the extended Fourier amplitude sensitivity testing(FAST)method to inflated,axial stretched,and residually stressed cylinders

This paper is dedicated to applying the Fourier amplitude sensitivity test(FAST)method to the problem of mixed extension and inflation of a circular cylindrical tube in the presence of residual stresses.The metafunctions and the Ishigami function are considered in the sensitivity analysis(SA).The effects of the input variables on the output variables are investigated,and the most important parameters of the system under the applied pressure and axial force such as the axial stretch and the azimuthal stretch are determined.

THE SENSITIVITY TEST STUDY OF AIR-SEA SURFACE FLUX MODEL

In this article, a sensitivity test of air-sea surface flux model was carried out with the field observation data of Project “South China Sea Air-Sea Flux Measurement in 2000”. The results show that sensible heat fluxes are sensitive to observation errors, increasing the error of model calculation; In contrast, the latent heat flux and momentum flux are not as sensitive to observation errors as the sensible heat, and their calculated results are reliable. The test result also verifies the rationality of the surface flux values calculated and the conclusions can be used to detect errors in observed data.

Isolation and Identification of Pseudomonas solanacearum and Its Drug Sensitivity Test

Ten pathogenic strains were isolated fi-om gingers infected by blast, and were identified by substrate utilization test and biochemical test. The identifica- tion results showed that these ten strains accorded with the basic characteristics of Pseudomonas solanacearum. Drug sensitivity test of ten strains was carried out, and prevention agents were screened to provide an experimental basis for the control of ginger blast.

Drug Sensitivity Test and Regression Verification of Escherichia coli from Rex Rabbit

[ Objectives] The paper aimed to select drugs reasonably for treatment of rex rabbit colibacillosis, and to isolate the pathogenicity of Escherichia coll. [ Methods ] Pathogen isolation, drug sensitivity test and pathogen regression test were performed with rex rabbits killed by E. coli in clinic. [ Results] The isolate was E. coli 0-23, susceptible to amikacin and cefotaxime sodium; when the challenge dose was 1.0 mL/rabbit （about one billion E. coli）, the test animal would discharge mucous feces. [ Conclusions] The results provided model support for clinical medicine selection against rex rabbit colibacillosis.

Isolation, Identification and Drug Sensitivity Test of a Pathogenic Escherichia coli Strain from Minks with Diarrhea

[Objectives] The study aimed to identify the pathogen that causes diarrhea in minks. [Methods] Liver tissues were aseptically collected from dead minks with diarrhea. By bacterial isolation and culture,morphological observation,biochemical test and pathogenicity test,the isolated strain was identified as pathogenic E. coli. [Results]The pathogen causing diarrhea in minks was confirmed as a pathogenic E. coli strain. Drug sensitivity test indicated that the isolated pathogenic E. coli strain was highly sensitive to ceftazidime,cefotaxime,enrofloxacin,florfenicol and cephradine,moderately sensitive to ampicillin,ciprofloxacin,amikacin,doxycycline,lincomycin and gentamycin,and resistant to amoxycillin,neomycin,spectinomycin,polymyxin and penicillin. [Conclusions] This study provided reference for the prevention and control of abortion in female minks in Qinhuangdao region.

NEW ANTIMICROBIAL SENSITIVITY TESTS OF BIOFILM OF STREPTOCOCCUS MUTANS IN ARTIFICIAL MOUTH MODEL

Objective To develop a new antimicrobial sensitivity test model for oral products in vitro.Methods A biofilm artificial mouth model for antimicrobial sensitivity tests was established by modifying the LKB chromatography chamber. Using sodium fluoride and Tea polyphenol as antimicrobial agent and Streptococcus mu-tans as target, sensitivity tests were studied. Results The modeling biofilm assay resulted in a MIC of 1. 28mg/ ml for fluoride against S. mutans, which was 32 times the MIC for broth maco-dilution method. The differential resistance of bacteria bioflim to antimicrobial agent relative to planktonic cells was also demonstrated. Conclusion The biofilm artificial mouth model may be useful in oral products test.

The Effect of the Atmospheric and Cloud Properties on the Retrieval of Fog Microphysics at Night for MSG/SEVIRI: A Sensitivity Test Using LibRadtran

The simplest way of building a look-up table (LUT) for the retrieval of cloud microphysical properties is to use a standard atmospheric profile and vertically uniform cloud microphysics. Such an assumption has been demonstrated to be incoherent with in-cloud observations. This paper aims to show the effect of some atmospheric conditions associated with fog as well as its macro-and microstructure on brightness temperature (BT) for the MSG/ SEVIRI satellite using libRadtran. The sensitivity tests were performed by gradually changing some features from the initial data, such as cloud cover, total water vapor column, thermal inversion intensity, fog depth, fog microstructure, and others. The results revealed that some variables can cause significant variations on BT and, consequently, discrepancies in the retrieval of fog microphysical properties. Also, a variation as high as 0.5°C was found on BT just by switching uniform to the non-uniform profile of fog microphysics depending on the channel, the droplet size, and optical thickness.

Test and Study of the Rock Pressure Sensitivity for KeLa-2 Gas Reservoir in the Tarim Basin

KeLa-2 gas reservoir is the largest uncompartimentalized gas field so far discovered in China, with a reserve of hundreds of billions of cubic meters of dry gas. It has such features as extremely long interval (550m), high pressure (74.5MPa) and pressure coefficient (2.022). Gas reservoirs with a pressure coefficient of over 2.0 are not commonly found. The abnormal high-pressure reservoirs are quite different in characteristic and performance during the process of depletion exploitation. Therefore, it is necessary to know the property of pressure sensitivity for this abnormal high-pressure reservoir. The aim of this paper is to test the reservoir pressure sensitivity and to analyze its effect on the deliverability of gas. Through some experiments, the permeability change with the confining pressure of rock samples from KeLa-2 abnormal high-pressure gas reservoir is measured. A power function is used to match the measured data, and to derive an empirical equation to describe the change of permeability through the change of the reservoir pressure or effective overburden pressure. Considering the permeability change during the development of reservoirs, a conventional deliverability equation is modified, and the deliverability curve for KeLa-2 gas reservoir is predicted. The research indicates that the extent of the pressure sensitivity of rock samples from KeLa-2 is higher than that from the Daqing oilfield. KeLa-2 reservoir rock has the feature of an undercompaction state. The pressure sensitivity of a reservoir may decrease the well deliverability. It is concluded that for KeLa-2 reservoir the predicted absolute open flow (AOF), when the pressure sensitivity is taken into account, is approximately 70% of the AOF when permeability is constant and does not change with pressure.

Distribution of pathogenic bacteria and antimicrobial sensitivity of eye infections in Suzhou

AIM:To investigate the types of bacteria in patients with eye infections in Suzhou and their drug resistance to commonly used antibacterial drugs.METHODS:The clinical data of 155 patients were retrospectively collected in this study,and the pathogenic bacteria species and drug resistance of each pathogenic bacteria were analyzed.RESULTS:Among the 155 patients(age from 12 to 87 years old,with an average age of 57,99 males and 56 females)with eye infections(160 eyes:74 in the left eye,76 in the right eye and 5 in both eyes,all of which were exogenous),71(45.81%)strains were gram-positive bacteria,23(14.84%)strains were gram-negative bacteria and 61(39.35%)strains were fungi.Gram-positive bacteria were highly resistant to penicillin and erythromycin(78.87%and 46.48%respectively),but least resistant to vancomycin at 0.Gram-negative bacteria were highly resistant to cefoxitin and compound sulfamethoxazole(100%and 95.65%respectively),but least resistant to meropenem at 0.Comparison of the resistance of gram-positive and gram-negative bacteria to some drugs revealed statistically significant differences(P<0.05)in the resistance of both to cefoxitin,cotrimoxazole,levofloxacin,cefuroxime,ceftriaxone and ceftazidime,and both had higher rates of resistance to gram-negative bacteria than to gram-positive bacteria.The distribution of bacterial infection strains showed that Staphylococcus epidermidis was the most common strain in the conjunctiva,cornea,aqueous humor or vitreous body and other eye parts.Besides,Fusarium and Pseudomonas aeruginosa were also among the most common strains of conjunctival and corneal infections.CONCLUSION:Gram-positive bacteria are the dominant bacteria in eye infections,followed by gram-negative bacteria and fungi.Considering the resistance of gramnegative bacteria to multiple drugs,monitoring of bacteria should be strengthened in eye bacterial infections for effective prevention and control to reduce complications caused by eye infections.

Age norms for grating acuity and contrast sensitivity measured by Lea tests in the first three years of life

AIM： To determine age norms in the first three years of life for grating visual acuity and contrast sensitivity obtained with Lea grating test and Hiding Heidi low contrast face test. METHODS： Lea grating test was used to estimate binocular grating acuity and Hiding Heidi low contrast face test was used to estimate contrast sensitivity in 600 healthy infants and children. Age ranged from 3 to 36 mo subdivided into 12 groups subjected for full ophthalmologic and pediatric examinations.RESULTS： The grating acuity developed along the first three years of life. It ranged from 1.88±0.32 c/d at 3mo to 30.95±0.77 c/d at 36 mo. The most rapid development was during the first 12 mo and the slowest development was from 30 to 36 mo. The contrast sensitivity showed rapid development in the first two years of life. Its mean value ranged from 4.23±1.17 at 3mo to 78.26±8.21 at 24 mo. It was constant at the highest score（80） thereafter.CONCLUSION： Age norms for grating acuity along with contrast sensitivity offer a more comprehensive measure of spatial vision and should be incorporated in clinical practice for better visual assessment in preverbal and nonverbal children.

The Changes of Baroreflex Sensitivity During Head-up Tilt Test and Its Clinical Significance in the Patients with Vasovagal Syncope

Objective To study thechanges of baroreflex sensitivity (BRS) during head - up tilt test (HUT) in patients with vasovagal syncope (VS), and to examine the relationship between baroreflex sensitivity and neurohormonal factors. Furthermore, to investigate the effects of the changes of BRS on VS. Methods Forty - two patients with unexplained syncope (Among the 42 patients, there were 22 patients with positive HUT and 20 patients with negative HUT respectively) and 20 healthy volunteers (with negative HUT) underwent passive head - up tilt testing, Ante-cubital vein blood samples were taken before and after HUT, or at syncope. The fasting plasma endothelin , serum nitric oxide (NO), serum NE were measured, the BRS was assessed on the basis of the linear regression slope the RR interval versus systolic arterial blood pressure during the increment in blood pressure after intravenous administration of phenylephrine. Results (1) During the syncope, the BRS significantly reduced in HUT(+) group than baseline. At the end of tilt, the level of plasma ET, serum NO in patients with positive HUT significantly increased compared with baseline or normal controls, and the plasma concentration of NE also had the trend of increase. (2) By multiple regression analysis, a significant negative correlation was found between baroreceptor sensitivity and the plasma ET, NO at the end of HUT in patients with positive HUT, but there was no relationship between BRS and NE. Conclusions During the syncope occure, the BRS in patients with VS decreased significantly compared with normal controls. The abnormal plasma ET, NO concen-tration might contribute to the mechanism of VS.

Using a Modified Soil-Plant-Atmosphere Scheme (MSPAS) to Study the Sensitivity of Land Surface and Boundary Layer Processes to Soil and Vegetation Conditions

A series of sensitivity tests are performed to test the stability and sensibility of the Modified Soil-Plant-Atmosphere Scheme (MSPAS), which was wholly introduced in a previous paper. The numerical simulation results from the experiments show good agreement with physical reality. Besides, some of the results are illuminating. Together with the first paper, it is concluded that MSPAS is a simple but effective model, and it is practically valuable in the research work of desertification control and reforestation in China.

Six-year analysis of key monitoring for bacterial strain distribution and antibiotic sensitivity in a hospital

BACKGROUND With the widespread use of antimicrobial drugs,bacterial resistance has become a significant problem,posing a serious threat to public health.The prevalence of clinical infection strains in hospitals and their drug sensitivities are key to the appropriate use of antibiotics in clinical practice.AIM To identify prevalent bacteria and their antibiotic resistance profiles in a hospital setting,thereby guiding effective antibiotic usage by clinicians.METHODS Specimens from across the institution were collected by the microbiology laboratory.The VITEK 2 compact fully automatic analyzer was used for bacterial identification and antibiotic sensitivity testing,and the WHONET5.6 software was utilized for statistical analysis.RESULTS A total of 12062 bacterial strains of key monitoring significance were detected.Staphylococcus aureus demonstrated widespread resistance to penicillin,but none of the strains were resistant to vancomycin or linezolid.Moreover,219 strains of methicillin-resistant coagulase-negative staphylococci and 110 strains of methicillin-resistant Staphylococcus aureus were detected.Enterococcus faecalis showed moderate resistance to the third-generation quinolones ciprofloxacin and levofloxacin,but its resistance to nitrofurantoin and tetracycline was low.Enterococcus faecium displayed significantly lower resistance to third-and fourthgeneration quinolones than Enterococcus faecalis.The resistance of two key monitoring strains,Escherichia coli and Klebsiella pneumoniae,to piperacillin/tazobactam was 5%-8%.However,none of the Escherichia coli and Klebsiella pneumoniae strains were resistant to meropenem.The resistance of Acinetobacter baumannii to piperacillin/sulbactam was nearly 90%.Nonetheless,the resistance to tigecycline was low,and Pseudomonas aeruginosa demonstrated minimal resistance in the antibiotic sensitivity test,maintaining a resistance of<10%to the cephalosporin antibiotics cefotetan and cefoperazone over the last 6 years.The resistance to amikacin remained at 0.2%over the past 3 years.CONCLUSION Our hospital’s overall antibiotic resistance rate was relatively stable from 2017 to 2022.The detection rates of key monitoring strains are reported quarterly and their resistance dynamics are monitored and communicated to the entire hospital,which can guide clinical antibiotic selection.

Improving treatment plan and mental health in children with abdominal infection for broad-spectrum bacterial infections

BACKGROUND Pediatric abdominal infection is a common but serious disease that requires timely and effective treatment.In surgical treatment,accurate diagnosis and rational application of antibiotics are the keys to improving treatment effects.AIM To investigate the effect of broad-spectrum bacterial detection on postoperative antibiotic therapy.METHODS A total of 100 children with abdominal infection who received surgical treatment in our hospital from September 2020 to July 2021 were grouped.The observation group collected blood samples upon admission and sent them for broad-spectrum bacterial infection nucleic acid testing,and collected pus or exudate during the operation for bacterial culture and drug sensitivity testing;the control group only sent bacterial culture and drug sensitivity testing during the operation.RESULTS White blood cell count,C-reactive protein,procalcitonin,3 days after surgery,showed better postoperative index than the control group(P<0.05).The hospital stay in the observation group was significantly shorter than that in the control group.The hospitalization cost in the observation group was significantly lower than that in the control group,and the difference between the two groups was statistically significant(P<0.05).CONCLUSION Early detection of broad-spectrum bacterial infection nucleic acids in pediatric abdominal infections can help identify pathogens sooner and guide the appropriate use of antibiotics,improving treatment outcomes and reducing medical costs to some extent.

Comparison of Antibacterial Activities of Six Bacillus amyloliquefaciens Strains

In this study, six bacterial strains were isolated from the sediment, probiotic fermentation products, and lake sediments, they were identified as Bacillus amyloliquefaciens using genetic evolution analysis, which were named B3, B4, B5, XD3, YF6, and YF8. The comparison of the antibacterial activity, hemolytic activity, and antibiotic sensitivity of six Bacillus amyloliquefaciens strains laid a foundation for the development and application of antimicrobial peptide products. A surface activity assay was used to determine the production of biosurfactants in six Bacillus amyloliquefaciens strains. With Staphylococcus aureus and Escherichia coli as indicator bacteria, their antibacterial activity was determined using the agar diffusion method;the same diffusion method was used to determine the antibiotic susceptibility of Bacillus amyloliquefaciens. The results showed that the six Bacillus amyloliquefaciens strains had obvious biosurface activity, and the bacteria inhibited Staphylococcus aureus and Escherichia coli, from strong to weak: YF8, XD3, B3, B4, YF6, and B5. Strain YF8 had the best broad-spectrum bacteriostatic effect, followed by strain XD3. All Bacillus amyloliquefaciens strains were susceptible to 16 common drugs, except for Bacillus amyloliquefaciens strain YF8, which was intermediate to neomycin. The study shows that Bacillus amyloliquefaciens and secondary metabolites have the ability to produce a variety of active peptides, exert a certain inhibitory effect on common pathogens, and have the potential to develop as animal probiotics.

Screening and Efficacy Observation of Traditional Chinese Medicine Against Chicken Pathogenic Escherichia coli

[ Objective] The aim of this study was to lay a foundation for obtaining effective traditional Chinese medicine preparations controlling chicken colibacillosis. [ Method] With the western medicine Lomeflbxacin Hydrochloride and Sodium Chloride Injection as the control, drug sensitivity tests of 15 single traditional Chinese herbs and seven self-made Chinese herbal compounds were conducted. Subsequently, the single traditional Chinese herbs presenting good in vitro antibacterial effects against Escherichia coli and all the seven compounds were given to the chicks artificially infected with E. coil to observe their efficacies. [ Result] The results of drug sensitivity tests showed that all the 15 traditional Chinese herbs had certain antibacterial effect against E. coli, among which aloe, pomegranate rind and radix sanguisorbae were the best. Compound 1, 2 and 5 had good antibacterial effects in vitro. By in vivo test, traditional Chinese medicines showed superior efficacies on infected chicks than western medicine, and compound 1 and 2 performed well. [ConclusiOn] Traditional Chinese medicine preparations are good at controlling Chicken colibacillosis, but their bacteriostasis mechanism in vivo was different with that in vitro.

Percentiles Estimation for a Response Distribution

To estimate percentiles of a response distribution, the transformed response rule of Wetherill and Robbins-Monro sequential design were proposed under Log-Logistic model. Based on responses data, a necessary and sufficient condition for the existence of maximum likelihood estimators and then the calculating formula were presented. After a simulation study, the proposed approach was applied to 65# detonator. Numerical results showed that estimators of percentiles from the proposed approach are robust to the parametric models lacking information on the original response distribution.

Diagnosis and Treatment of Hemorrhagic Pneumonia of Mink

[Objective] The paper was to diagnose and treat hemorrhagic pneumonia of mink. [Method] The diseased minks bred by one raiser in Huangtian Village of Changli County in Qinhuangdao City were used as experimental materials. It was necropsied, and its tissues such as liver, kidney, spleen and lung were taken under aseptic condition, then inoculated into general nutrition agar and blood agar. The bacteria were isolated and purified, and the strains obtained were carried out animal inoculation test and drug sensitivity test. Finally, the relevant treatment and preven- tive measures were put forward. [Conclusion] The bacteria isolated were detected to be negative by Gram staining; it formed hemolysis ring in blood agar; the bacteria could ferment glucose and xylose, which could not ferment sucrose, lactose, maltose and esculin; it was positive in catalase and oxidase test, and negative in urease test and hydrogen sulfide test; so the strain was indentified to be Pseudomonas aerugi- nosa. Drug sensitivity test showed that the bacteria were highly sensitive to ciprofloxacin, which had tolerance to cefoxitin. [Conclusion] The study laid foundation for diagnosis and treatment of hemorrhagic pneumonia of mink.

Simulations of a Hydrological Model as Coupled to a Regional Climate Model

Considering a detailed hydrologic model in the land surface scheme helps to improve the simulation of regional hydro-climatology. A hydrologic model, which includes spatial heterogeneities in precipitation and infiltration, is constructed and incorporated into the land surface scheme BATS. Via the coupled-model (i.e., a regional climate model) simulations, the following major conclusions are obtained: the simulation of surface hydrology is sensitive to the inclusion of heterogeneities in precipitation and infiltration; the runoff ratio is increased after considering the infiltration heterogeneity, a result which is more consistent with the observations of surface moisture balance over humid areas; the introduction of the parameterization of infiltration heterogeneity can have a greater influence on the regional hydro-climatology than the precipitation heterogeneity; and the consideration of the impermeable fraction for the region reveals some features that are closer to the trend of aridification over northern China.