A field experiment was conducted to investigate the fate of ^15N-labeled urea and its residual effect under the winter wheat （Triticum aestivum L.） and summer maize （Zea mays L.） rotation system on the North China...A field experiment was conducted to investigate the fate of ^15N-labeled urea and its residual effect under the winter wheat （Triticum aestivum L.） and summer maize （Zea mays L.） rotation system on the North China Plain. Compared to a conventional application rate of 360 kg N ha^-1 （N360）, a reduced rate of 120 kg N ha^-1 （N120） led to a significant increase （P 〈 0.05） in wheat yield and no significant differences were found for maize. However, in the 0-100 cm soil profile at harvest, compared with N360, N120 led to significant decreases （P 〈 0.05） of percent residual N and percent unaccounted-for N, which possibly reflected losses from the managed system. Of the residual fertilizer N in the soil profile, 25.6%-44.7% and 20.7%-38.2% for N120 and N360, respectively, were in the organic N pool, whereas 0.3%-3.0% and 11.2%-24.4%, correspondingly, were in the nitrate pool, indicating a higher potential for leaching loss associated with application at the conventional rate. Recovery of residual N in the soil profile by succeeding crops was less than 7.5% of the applied N. For N120, total soil N balance was negative; however, there was still considerable mineral N （NH4^＋-N and NO3^--N） in the soil profile after harvest. Therefore, N120 could be considered ngronomically acceptable in the short run, but for long-term sustainability, the N rate should be recommended based on a soil mineral N test and a plant tissue nitrate test to maintain the soil fertility.展开更多
In this paper, the role of mixed amino acids in nitrate uptake and assimilation was evaluated in leafy radish by using ^15N labeled nitrate. The mixtures of alanine, β-alanine, aspartic acid, asparagines, glutamic ac...In this paper, the role of mixed amino acids in nitrate uptake and assimilation was evaluated in leafy radish by using ^15N labeled nitrate. The mixtures of alanine, β-alanine, aspartic acid, asparagines, glutamic acid, glutamine, and glycine were sprayed to plant leaf two or four times. The activity of the enzymes related to the process of NO3- reduction （nitrate reductase, nitrite reductase and glutamine synthetase） was affected differently depending on the application rate of mixed amino acids. Applying mixed amino acids increased the fresh weight, dry weight, and N yield. The NO3 content was reduced to 24-38%, but no significant differences were observed in amino acids and proteins. In addition, the nitrogen derived from fertilizer and the ^15N-NO3-recovery rate increased to 2-8% and 15-47%, respectively. These results strongly suggest that the positive effect of mixed amino acids on nitrate uptake and assimilation might be attributed to the regulation on NO3- uptake and assimilation, but not to the preference for amino acids as sources of reduced nitrogen.展开更多
The partitioning of nitrogen deposition among forest soil （including forest floor）, leachate and above- and belowground biomass of pot cultured beech seedlings in comparison to non-cultured treatments were investiga...The partitioning of nitrogen deposition among forest soil （including forest floor）, leachate and above- and belowground biomass of pot cultured beech seedlings in comparison to non-cultured treatments were investigated by adding 1.92 g.m^-2 ^15N tracer in throughfall for two successive growing seasons at a greenhouse experiment. Ammonium and nitrate depositions were simulated on four treatments （cultured and non-cultured） and each treatment was labeled with either ^15N-NH4^＋ or ^15N-NO3^-. Total recovery rates of the applied ^15N in the whole system accounted for 74.9% to 67.3% after ^15N-NH4^＋ and 85.3% to 88.1% after ^15N-NO3^-in cultured and non-cultured treatments, respectively. The main sink for both ^15N tracers was the forest soil （including forest floor）, where 34.6% to 33.7% of ^15N-NH4^＋ and 13.1% to 9.0% of ^15N-NO3^-were found in cultured and non-cultured treatments, respectively, suggesting strong immobilization of both N forms by hetero- trophic microorganisms. Nitrogen immobilization by microorganisms in the forest soil （including forest floor） was three times higher when ^15N-NH4^＋ was applied compared to ^15N-NO3^-. The preferential heterotrophic use of ammonium resulted in a two times higher retention of deposited ^15N-NH4^＋ in the forest soil as compared to plants. In contrast, nitrate immobilization in the forest soil was lower compared to plants, although statistically it was not significantly different. In total the immobilization of ammonium in the plant-soil system was about 60% higher than nitrate, indicating the importance of the N-forms deposition for retention in forest ecosystems.展开更多
The objective of this study was to determine the efficiency of different plant systems in capturing deep soil nitrate （NO3-） to reduce NO3- leaching in a field plot experiment using 15N labelling. The study was cond...The objective of this study was to determine the efficiency of different plant systems in capturing deep soil nitrate （NO3-） to reduce NO3- leaching in a field plot experiment using 15N labelling. The study was conducted on a calcareous alluvial soil on the North China Plains and the plant systems evaluated included alfalfa （Medicago sativa）, American black poplar （Populus nigra） and cocksfoot （Dactylis）. ^15N-labelled N fertilizer was injected to 90 cm depth to determine the recovery of ^15N by the plants. With conventional water and nutrient management, the total recovery of ^15N-labeled NO3--N was 23.4% by alfalfa after two consecutive growth years. The recovery was significantly higher than those by American black poplar （12.3%） and cocksfoot （11.4%）. The highest proportion of soil residual ~SN from the labeled fertilizer N （%Ndff） was detected around 90 cm soil depth at the time of the 1st year harvest and at 110-130 cm soil depth at time of the 2nd year harvest. Soil %Ndff in 0-80 cm depth was significantly higher in the alfalfa treatment than those in all the other treatments. The soil %Ndff below 100 cm depth was much lower in the alfalfa than those in all the other treatments. These results indicated that ^15N leaching losses in the alfalfa treatment were significantly lower than by those in the black poplar and cocksfoot treatments, due to the higher root density located in nitrate labeling zone of soil profile. In conclusion, alfalfa may be used as a plant to capture deep soil NO3- left from previous crops to reduce NO3- leaching in high intensity crop cultivation systems of North China Plain.展开更多
Three goats were fed with ^15N-labelled rice straw to study the characteristics of digestion,assimilation,transformation and excretion of C and N compounds from rice straw.It was shown that the amount of ^15N transfor...Three goats were fed with ^15N-labelled rice straw to study the characteristics of digestion,assimilation,transformation and excretion of C and N compounds from rice straw.It was shown that the amount of ^15N transformed into the bodies of the two slaughtered goats accounted for 38.5 and 23.6% of the total amount of ^15N deposition of the experimental diet taken by each goat.The ^15N excreted through the feces and urine for the three goats accounted for 34.8,33.2 and 33.9% of the total amount of ^15N deposition in the feed of the 3 goats.The recovery of total ^15N for the two slaughtered goats were 73.3 and 57.5%,with the corresponding rates of ^15N loss 26.7 and 42.5% respectively.The digestibilities of total amino acids for Goats 1 and 3 were 68.7 and 54.0%,and the digestibilties of carbohydrates for the two goats were 74.8 and 67.7% respectively.展开更多
Nitrogen fixation is one of the most important sources of new nitrogen in the ocean and thus profoundly affects the nitrogen and carbon biogeochemical processes.The distribution,controlling factors,and flux of N2 fixa...Nitrogen fixation is one of the most important sources of new nitrogen in the ocean and thus profoundly affects the nitrogen and carbon biogeochemical processes.The distribution,controlling factors,and flux of N2 fixation in the global ocean remain uncertain,partly because of the lack of methodological uniformity.The^(15)N_(2)tracer assay(the original bubble method→the^(15)N_(2)-enriched seawater method→the modified bubble method)is the mainstream method for field measurements of N2 fixation rates(NFRs),among which the original bubble method is the most frequently used.However,accumulating evidence has suggested an underestimation of NFRs when using this method.To improve the availability of previous data,we compared NFRs measured by three^(15)N_(2)tracer assays in the South China Sea.Our results indicate that the relationship between NFRs measured by the original bubble method and the^(15)N_(2)-enriched seawater method varies obviously with area and season,which may be influenced by incubation time,diazotrophic composition,and environmental factors.In comparison,the relationship between NFRs measured by the original bubble method and the modified bubble method is more stable,indicating that the N2 fixation rates based on the original bubble methods may be underestimated by approximately 50%.Based on this result,we revised the flux of N2 fixation in the South China Sea to 40 mmol/(m2·a).Our results improve the availability and comparability of literature NFR data in the South China Sea.The comparison of the^(15)N_(2)tracer assay for NFRs measurements on a larger scale is urgently necessary over the global ocean for a more robust understanding of the role of N2 fixation in the marine nitrogen cycle.展开更多
Nitrogenous fertilisers are under consideration for promoting the growth of nursery-reared hoop pine （Araucaria cunninghamii Alton ex A. Cunn） seedlings in the establishment phase of second rotation （2R） plantatio...Nitrogenous fertilisers are under consideration for promoting the growth of nursery-reared hoop pine （Araucaria cunninghamii Alton ex A. Cunn） seedlings in the establishment phase of second rotation （2R） plantations. Using ^15N- labelled fertilisers, we investigated the effect of different forms （ammonium sulphate, ammonium nitrate, potassium nitrate and urea） and rates of application （0, 150 and 300 mg N kg^-1 dried soil） of fertilisers on the growth, ^15N recovery and carbon isotope composition （δ^13C） of hoop pine seedlings in a 12-month glasshouse trial in southeast Queensland, Australia. The ^15N-labelled fertilisers were applied to nursery-reared hoop pine seedlings, which were then grown in pots, containing ca. 1.2 kg dried soil, under well watered conditions for 12 months. Four seedlings from each treatment were harvested at 4-month intervals, divided into roots, stem and foliage, with a further subdivision for new and old foliage, and then analysed for ^15N, total N, δ^13C and total C. There was no significant response in the seedling growth to the form or rate of application of nitrogen （N） fertiliser within the 12-month period, indicating that the seedlings did not experience N deficiency when grown on second rotation hoop pine soils. While the combined ^15N recovery from soil and plant remained at around 70% throughout the experiment, the proportion of ^15N recovered from the plants increasing steadily over time. Nitrate containing fertilisers at 150 mg N kg^-1 soil gradually increased seedling foliage δ^13C over the 12-month period, indicating an increase in seedling water use efficiency.展开更多
Water-soluble, nondialyzable Maillard polymers were prepared by reacting D-xylose with 15N-glycine (and/or glycine) at 68 ℃ and pH 8.0 at equimolar concentrations of 1, 0.5 and 0.1 mol L-1, respectively,for 13 days a...Water-soluble, nondialyzable Maillard polymers were prepared by reacting D-xylose with 15N-glycine (and/or glycine) at 68 ℃ and pH 8.0 at equimolar concentrations of 1, 0.5 and 0.1 mol L-1, respectively,for 13 days and partitioned into acid-insoluble (MHA) and acid-soluble (MFA) fractions. The nitrogen forms in these polymers were studied by using the 15N cross polarization-magic angle spinning nuclear magnetic resonance (CPMAS NMR) technique in combination with chemical methods. The 15N nuclear magnetic resonance (NMR) data showed that while the yield, especially the MHA/MFA ratio, varied considerably with the concentrations of the reactants, the nitrogen distribution patterns of these polymers were quite similar.From 65% to 70% of nitrogen in them was in the secondary amide and/or indole form with 24%~25% present as aliphatic and/or aromatic ammes and 5% to 11% as pyrrole and/or pyrrole-like nitrogen. More than half (50%~77%) of the N in these polymers were nonhydrolyzable. The role of Maillard reaction in the formation of nonhydrolyzable nitrogen in soil organic matter is discussed.展开更多
基金Project supported by the National Natural Science Foundation of China (Nos. 40571071, 30390080 and 30370287)the Program for Changjiang Scholars and Innovative Research Team in University (No. IRT0511).
文摘A field experiment was conducted to investigate the fate of ^15N-labeled urea and its residual effect under the winter wheat （Triticum aestivum L.） and summer maize （Zea mays L.） rotation system on the North China Plain. Compared to a conventional application rate of 360 kg N ha^-1 （N360）, a reduced rate of 120 kg N ha^-1 （N120） led to a significant increase （P 〈 0.05） in wheat yield and no significant differences were found for maize. However, in the 0-100 cm soil profile at harvest, compared with N360, N120 led to significant decreases （P 〈 0.05） of percent residual N and percent unaccounted-for N, which possibly reflected losses from the managed system. Of the residual fertilizer N in the soil profile, 25.6%-44.7% and 20.7%-38.2% for N120 and N360, respectively, were in the organic N pool, whereas 0.3%-3.0% and 11.2%-24.4%, correspondingly, were in the nitrate pool, indicating a higher potential for leaching loss associated with application at the conventional rate. Recovery of residual N in the soil profile by succeeding crops was less than 7.5% of the applied N. For N120, total soil N balance was negative; however, there was still considerable mineral N （NH4^＋-N and NO3^--N） in the soil profile after harvest. Therefore, N120 could be considered ngronomically acceptable in the short run, but for long-term sustainability, the N rate should be recommended based on a soil mineral N test and a plant tissue nitrate test to maintain the soil fertility.
基金the Science Foundation of Zhejiang Forestry University, China (2006FR011)
文摘In this paper, the role of mixed amino acids in nitrate uptake and assimilation was evaluated in leafy radish by using ^15N labeled nitrate. The mixtures of alanine, β-alanine, aspartic acid, asparagines, glutamic acid, glutamine, and glycine were sprayed to plant leaf two or four times. The activity of the enzymes related to the process of NO3- reduction （nitrate reductase, nitrite reductase and glutamine synthetase） was affected differently depending on the application rate of mixed amino acids. Applying mixed amino acids increased the fresh weight, dry weight, and N yield. The NO3 content was reduced to 24-38%, but no significant differences were observed in amino acids and proteins. In addition, the nitrogen derived from fertilizer and the ^15N-NO3-recovery rate increased to 2-8% and 15-47%, respectively. These results strongly suggest that the positive effect of mixed amino acids on nitrate uptake and assimilation might be attributed to the regulation on NO3- uptake and assimilation, but not to the preference for amino acids as sources of reduced nitrogen.
基金supported by ibw, Goet-tingen University, Germany
文摘The partitioning of nitrogen deposition among forest soil （including forest floor）, leachate and above- and belowground biomass of pot cultured beech seedlings in comparison to non-cultured treatments were investigated by adding 1.92 g.m^-2 ^15N tracer in throughfall for two successive growing seasons at a greenhouse experiment. Ammonium and nitrate depositions were simulated on four treatments （cultured and non-cultured） and each treatment was labeled with either ^15N-NH4^＋ or ^15N-NO3^-. Total recovery rates of the applied ^15N in the whole system accounted for 74.9% to 67.3% after ^15N-NH4^＋ and 85.3% to 88.1% after ^15N-NO3^-in cultured and non-cultured treatments, respectively. The main sink for both ^15N tracers was the forest soil （including forest floor）, where 34.6% to 33.7% of ^15N-NH4^＋ and 13.1% to 9.0% of ^15N-NO3^-were found in cultured and non-cultured treatments, respectively, suggesting strong immobilization of both N forms by hetero- trophic microorganisms. Nitrogen immobilization by microorganisms in the forest soil （including forest floor） was three times higher when ^15N-NH4^＋ was applied compared to ^15N-NO3^-. The preferential heterotrophic use of ammonium resulted in a two times higher retention of deposited ^15N-NH4^＋ in the forest soil as compared to plants. In contrast, nitrate immobilization in the forest soil was lower compared to plants, although statistically it was not significantly different. In total the immobilization of ammonium in the plant-soil system was about 60% higher than nitrate, indicating the importance of the N-forms deposition for retention in forest ecosystems.
基金financially supported by the Provincial Natural Science Foundation of Hebei Province in China(C2006000491)the financial support from the National Natural Science Foundation of China(30571110,31172033)the National 863 Program of China(2012AA101403-3)
文摘The objective of this study was to determine the efficiency of different plant systems in capturing deep soil nitrate （NO3-） to reduce NO3- leaching in a field plot experiment using 15N labelling. The study was conducted on a calcareous alluvial soil on the North China Plains and the plant systems evaluated included alfalfa （Medicago sativa）, American black poplar （Populus nigra） and cocksfoot （Dactylis）. ^15N-labelled N fertilizer was injected to 90 cm depth to determine the recovery of ^15N by the plants. With conventional water and nutrient management, the total recovery of ^15N-labeled NO3--N was 23.4% by alfalfa after two consecutive growth years. The recovery was significantly higher than those by American black poplar （12.3%） and cocksfoot （11.4%）. The highest proportion of soil residual ~SN from the labeled fertilizer N （%Ndff） was detected around 90 cm soil depth at the time of the 1st year harvest and at 110-130 cm soil depth at time of the 2nd year harvest. Soil %Ndff in 0-80 cm depth was significantly higher in the alfalfa treatment than those in all the other treatments. The soil %Ndff below 100 cm depth was much lower in the alfalfa than those in all the other treatments. These results indicated that ^15N leaching losses in the alfalfa treatment were significantly lower than by those in the black poplar and cocksfoot treatments, due to the higher root density located in nitrate labeling zone of soil profile. In conclusion, alfalfa may be used as a plant to capture deep soil NO3- left from previous crops to reduce NO3- leaching in high intensity crop cultivation systems of North China Plain.
文摘Three goats were fed with ^15N-labelled rice straw to study the characteristics of digestion,assimilation,transformation and excretion of C and N compounds from rice straw.It was shown that the amount of ^15N transformed into the bodies of the two slaughtered goats accounted for 38.5 and 23.6% of the total amount of ^15N deposition of the experimental diet taken by each goat.The ^15N excreted through the feces and urine for the three goats accounted for 34.8,33.2 and 33.9% of the total amount of ^15N deposition in the feed of the 3 goats.The recovery of total ^15N for the two slaughtered goats were 73.3 and 57.5%,with the corresponding rates of ^15N loss 26.7 and 42.5% respectively.The digestibilities of total amino acids for Goats 1 and 3 were 68.7 and 54.0%,and the digestibilties of carbohydrates for the two goats were 74.8 and 67.7% respectively.
基金The National Natural Science Foundation of China under contract Nos 42076042 and 41721005the Fund of Ministry of Science and Technology of China under contract No.2017FY201403the Fund of China Ocean Mineral Resources R&D Association under contract No.DY135-13-E2-03.
文摘Nitrogen fixation is one of the most important sources of new nitrogen in the ocean and thus profoundly affects the nitrogen and carbon biogeochemical processes.The distribution,controlling factors,and flux of N2 fixation in the global ocean remain uncertain,partly because of the lack of methodological uniformity.The^(15)N_(2)tracer assay(the original bubble method→the^(15)N_(2)-enriched seawater method→the modified bubble method)is the mainstream method for field measurements of N2 fixation rates(NFRs),among which the original bubble method is the most frequently used.However,accumulating evidence has suggested an underestimation of NFRs when using this method.To improve the availability of previous data,we compared NFRs measured by three^(15)N_(2)tracer assays in the South China Sea.Our results indicate that the relationship between NFRs measured by the original bubble method and the^(15)N_(2)-enriched seawater method varies obviously with area and season,which may be influenced by incubation time,diazotrophic composition,and environmental factors.In comparison,the relationship between NFRs measured by the original bubble method and the modified bubble method is more stable,indicating that the N2 fixation rates based on the original bubble methods may be underestimated by approximately 50%.Based on this result,we revised the flux of N2 fixation in the South China Sea to 40 mmol/(m2·a).Our results improve the availability and comparability of literature NFR data in the South China Sea.The comparison of the^(15)N_(2)tracer assay for NFRs measurements on a larger scale is urgently necessary over the global ocean for a more robust understanding of the role of N2 fixation in the marine nitrogen cycle.
基金Project supported by a scholarship grant from the Cooperative Research Centre for Sustainable Production Forestry,Australia
文摘Nitrogenous fertilisers are under consideration for promoting the growth of nursery-reared hoop pine （Araucaria cunninghamii Alton ex A. Cunn） seedlings in the establishment phase of second rotation （2R） plantations. Using ^15N- labelled fertilisers, we investigated the effect of different forms （ammonium sulphate, ammonium nitrate, potassium nitrate and urea） and rates of application （0, 150 and 300 mg N kg^-1 dried soil） of fertilisers on the growth, ^15N recovery and carbon isotope composition （δ^13C） of hoop pine seedlings in a 12-month glasshouse trial in southeast Queensland, Australia. The ^15N-labelled fertilisers were applied to nursery-reared hoop pine seedlings, which were then grown in pots, containing ca. 1.2 kg dried soil, under well watered conditions for 12 months. Four seedlings from each treatment were harvested at 4-month intervals, divided into roots, stem and foliage, with a further subdivision for new and old foliage, and then analysed for ^15N, total N, δ^13C and total C. There was no significant response in the seedling growth to the form or rate of application of nitrogen （N） fertiliser within the 12-month period, indicating that the seedlings did not experience N deficiency when grown on second rotation hoop pine soils. While the combined ^15N recovery from soil and plant remained at around 70% throughout the experiment, the proportion of ^15N recovered from the plants increasing steadily over time. Nitrate containing fertilisers at 150 mg N kg^-1 soil gradually increased seedling foliage δ^13C over the 12-month period, indicating an increase in seedling water use efficiency.
文摘Water-soluble, nondialyzable Maillard polymers were prepared by reacting D-xylose with 15N-glycine (and/or glycine) at 68 ℃ and pH 8.0 at equimolar concentrations of 1, 0.5 and 0.1 mol L-1, respectively,for 13 days and partitioned into acid-insoluble (MHA) and acid-soluble (MFA) fractions. The nitrogen forms in these polymers were studied by using the 15N cross polarization-magic angle spinning nuclear magnetic resonance (CPMAS NMR) technique in combination with chemical methods. The 15N nuclear magnetic resonance (NMR) data showed that while the yield, especially the MHA/MFA ratio, varied considerably with the concentrations of the reactants, the nitrogen distribution patterns of these polymers were quite similar.From 65% to 70% of nitrogen in them was in the secondary amide and/or indole form with 24%~25% present as aliphatic and/or aromatic ammes and 5% to 11% as pyrrole and/or pyrrole-like nitrogen. More than half (50%~77%) of the N in these polymers were nonhydrolyzable. The role of Maillard reaction in the formation of nonhydrolyzable nitrogen in soil organic matter is discussed.
