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丛枝菌根对植物叶片δ^(15) N含量影响的数据挖掘分析
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作者 徐寿霞 《河南科技大学学报(自然科学版)》 CAS 北大核心 2024年第1期88-97,M0008,共11页
为明确丛枝菌根(AM)对植物叶片稳定氮同位素(δ^(15)N)含量的影响,探索丛枝菌根在调控植物应对全球变化方面的重要功能,通过数据挖掘,建立了AM植物和非AM(non-AM)植物叶片δ^(15)N含量数据库,探究了AM对植物叶片δ^(15)N含量的影响。结... 为明确丛枝菌根(AM)对植物叶片稳定氮同位素(δ^(15)N)含量的影响,探索丛枝菌根在调控植物应对全球变化方面的重要功能,通过数据挖掘,建立了AM植物和非AM(non-AM)植物叶片δ^(15)N含量数据库,探究了AM对植物叶片δ^(15)N含量的影响。结果表明:AM植物叶片δ^(15)N含量为0.86‰,显著高于non-AM植物叶片的0.50‰。丛枝菌根针对不同植物功能群而言,将植物的AM状况与植物的固氮状况和生长周期结合作为影响因素时,AM都显著影响着植物叶片的δ^(15)N含量。虽然AM没有显著提高固氮植物叶片δ^(15)N含量,但却把非固氮植物叶片δ^(15)N的含量从0.58‰提高到了0.99‰。AM使多年生植物叶片δ^(15)N含量的增加最显著,从0.49‰增加到了0.82‰。可见,丛枝菌根作为与植物长期共生进化的最广泛共生体,对植物叶片δ^(15)N含量有不同程度的影响。 展开更多
关键词 ^叶片δ^(15)n含量 丛枝菌根 功能型 固氮 计量分析
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小长山岛3种大型海藻组织碳、氮含量和δ^(15)N值的环境指示意义
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作者 赵玥茹 蔡恒江 +3 位作者 王选忠 刘龙强 单晓煊 胡思琪 《应用海洋学学报》 CAS CSCD 北大核心 2023年第4期667-674,共8页
大型海藻组织碳、氮含量及δ^(15)N值的变化对于指示营养盐来源及营养盐评价研究具有重要的意义。为探究大型海藻组织碳、氮含量及δ^(15)N值的变化特征,于2020年7月至2021年6月逐月分析了小长山岛潮间带孔石莼(Ulva pertusa)、角叉菜(C... 大型海藻组织碳、氮含量及δ^(15)N值的变化对于指示营养盐来源及营养盐评价研究具有重要的意义。为探究大型海藻组织碳、氮含量及δ^(15)N值的变化特征,于2020年7月至2021年6月逐月分析了小长山岛潮间带孔石莼(Ulva pertusa)、角叉菜(Chondrus ocellatus)、鼠尾藻(Sargassum thunbergii)3种大型海藻组织的碳、氮含量和δ^(15)N值的变化,并对其生长环境中海水的理化因子进行了测定。结果发现:孔石莼组织的氮含量与海水中的NO_(3)-N和DIN含量之间存在显著的相关性,角叉菜组织的氮含量与海水中的NH 3-N含量之间也存在显著的相关性。然而,大型海藻组织的碳含量和C/N比值与海水中的碳、氮营养盐之间并无显著的相关性。孔石莼组织的δ^(15)N值与海水中的NO_(3)-N和DIN含量之间也存在显著的相关性。因此,孔石莼适于用作NO_(3)-N和DIN来源的指示藻种。结果指示小长山岛潮间带海水中具有供大型海藻生长利用的充足的营养盐,其主要受到生活污水和养殖排放的影响。 展开更多
关键词 海洋生物学 大型海藻 碳、氮含量 ^δ^(15)n 氮营养盐
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几种饲用牧草的δ^(15)N值及其固氮能力初评 被引量:3
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作者 姚允寅 陈明 +4 位作者 马昌磷 王志东 侯景琴 张丽红 骆永云 《同位素》 CAS 北大核心 1992年第3期129-134,共6页
不同种、不同品种豆科牧草茎叶的δ^(15)N值相差甚大,表明了它们的固氮能力的明显不同。某些禾本科牧草茎叶的δ^(15)N值很低,接近大气N_2的δ^(15)N值(0‰),表明它们很可能通过联合固氮等途径获取生长所需的N素,值得进一步研究。
关键词 ^δ^(15)n 固氮作用 牧草
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同位素质谱仪测定小麦植株各器官δ^(15)N值的考察 被引量:2
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作者 谷淑波 宋雪皎 +1 位作者 王树芸 高居荣 《分析测试技术与仪器》 CAS 2018年第3期129-135,共7页
建立了15N标记的小麦植株各器官中δ^(15)N值的元素分析仪-同位素比质谱仪联用技术(EA-IRMS)的测定方法.对仪器的稳定性和线性进行了条件优化,标准气N_2的同位素比值的标准偏差为0.03‰,在同位素信号值1e^(-9)~1e^(-8)A范围内,总体线性... 建立了15N标记的小麦植株各器官中δ^(15)N值的元素分析仪-同位素比质谱仪联用技术(EA-IRMS)的测定方法.对仪器的稳定性和线性进行了条件优化,标准气N_2的同位素比值的标准偏差为0.03‰,在同位素信号值1e^(-9)~1e^(-8)A范围内,总体线性为0.009‰/n A.称取不同量的小麦粉标准物质(OAS/Isotope)测定δ^(15)N值,进样量为4 mg时标准偏差最小,为最佳称样量.对硫酸铵标准物质(IAEA-N2-6)、小麦粉标准物质和^(15)N标记小麦植株样品各器官进行测定,δ^(15)N值的标准偏差均小于5%,测定精度和准确度较好.方法为研究氮素在小麦体内运转及利用效率提供了有效的技术手段. 展开更多
关键词 元素分析仪-同位素比质谱仪联用技术 15n标记 小麦 ^δ^15n
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黄海小黄鱼不同组织中δ^(15)N的分布特征及其生态学意义 被引量:7
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作者 谭鲁玉 王玉堃 +3 位作者 唐学玺 戴芳群 孙耀 金显仕 《渔业科学进展》 CSCD 北大核心 2018年第3期30-35,共6页
本研究利用同位素质谱仪测定了黄海小黄鱼(Larimichthys polyactis)的肌肉、内脏、鱼鳃、鳞片和背鳍等不同组织器官中的氮稳定同位素的比值(δ^(15)N)。结果显示,内脏、鱼鳃、背鳍、鳞片与肌肉间δ^(15)N的平均值范围为10.29‰~10.72‰... 本研究利用同位素质谱仪测定了黄海小黄鱼(Larimichthys polyactis)的肌肉、内脏、鱼鳃、鳞片和背鳍等不同组织器官中的氮稳定同位素的比值(δ^(15)N)。结果显示,内脏、鱼鳃、背鳍、鳞片与肌肉间δ^(15)N的平均值范围为10.29‰~10.72‰,无显著差异(P>0.05);而耳石中的δ^(15)N值低于其他组织器官中的δ^(15)N值,其平均值为7.08‰,差异极显著(P<0.01),可见耳石与其他组织器官中δ^(15)N值的组成明显不同。Pearson相关分析表明,内脏、背鳍、鳞片、鱼鳃、耳石与肌肉中的δ^(15)N值呈极显著正相关,其Pearson相关系数均>0.72,表明其他组织器官中δ^(15)N值可替代肌肉研究鱼类营养等级和构建海洋食物网结构的方法具有可行性。 展开更多
关键词 ^δ^(15)n 组织器官 小黄鱼
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增温施肥对农田土壤有机碳和全氮含量及δ^(13)C、δ^(15)N值的影响 被引量:2
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作者 李佳珍 董文旭 +1 位作者 陈拓 胡春胜 《中国生态农业学报(中英文)》 CAS CSCD 北大核心 2022年第5期842-850,共9页
农田土壤是重要的碳氮库,对气候变化极其敏感,但土壤碳氮循环对气候变化的响应目前还不清楚。在全球变暖背景下,为了实现我国碳达峰、碳中和目标,研究增温对土壤有机碳、全氮含量及其碳氮同位素的影响具有重要的现实意义。本研究采用红... 农田土壤是重要的碳氮库,对气候变化极其敏感,但土壤碳氮循环对气候变化的响应目前还不清楚。在全球变暖背景下,为了实现我国碳达峰、碳中和目标,研究增温对土壤有机碳、全氮含量及其碳氮同位素的影响具有重要的现实意义。本研究采用红外辐射加热器模拟全球变暖,使5 cm土壤温度增加约2℃。通过测定灌溉前后土壤有机碳和全氮含量及δ^(13)C和δ^(15)N值的变化,研究增温、施氮和灌溉对华北平原小麦田土壤碳氮库的影响。试验共设4个处理:不施氮不增温(N0T0)、不施氮增温(N0T1)、施氮不增温(N1T0)和施氮增温(N1T1)。结果表明:灌溉前,增温降低了土壤有机碳含量,0~10 cm土层N1T1处理与不增温处理(N0T0和N1T0)之间差异显著(P<0.05),10~20 cm土层N1T1处理与其他处理差异均显著(P<0.05);灌溉后,增温虽有降低土壤有机碳含量的趋势,但差异不显著;施氮条件下,增温显著提升了δ^(13)C值(P<0.05)。增温降低了土壤全氮含量,并在灌溉前10~20 cm土层和灌溉后0~10 cm土层达显著水平(P<0.05);增温提升了土壤δ^(15)N值,灌溉前0~10 cm土层N0T0处理与增温处理(N0T1和N1T1)差异显著(P<0.05),灌溉后0~10 cm土层仅N0T1和N1T0处理间差异显著(P<0.05),而10~20 cm土层增温处理(N0T1和N1T1)均与N1T0处理差异显著(P<0.05)。同一处理同一时期,土壤有机碳和全氮的含量随土壤深度的增加而降低,δ^(13)C和δ^(15)N值随深度增加而升高,但土壤有机碳和δ^(13)C值的变化差异不显著,全氮含量和δ^(15)N值的变化差异显著(P<0.05)。土壤有机碳、全氮含量及δ^(13)C和δ^(15)N值在灌溉前后的差异均不显著。连续5年的增温施氮试验表明,未来气候变暖可能会加快土壤有机碳和全氮的分解,造成更多轻组有机碳损失。灌溉在短期内不会显著改变土壤碳氮含量及δ^(13)C和δ^(15)N值,但其长期影响还需进一步探究。此外,未来研究还应该重视多因素交互作用对土壤碳氮循环的影响。 展开更多
关键词 增温 施氮 土壤有机碳 土壤全氮 ^δ^(13)C值 ^δ^(15)n
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^(δ)15 N在有机农产品鉴别上的应用前景 被引量:2
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作者 马希贤 高源 +1 位作者 王会 胡国庆 《安徽农业科学》 CAS 2022年第2期15-18,共4页
有机农业在世界范围内正呈现快速发展的趋势,有机产品识别受到人们的普遍关注。目前已有研究者提出将产品^(15)N自然丰度(^(δ)15 N值)作为其鉴别指标。介绍了稳定性氮同位素的相关知识,解释了^(δ)15 N识别技术判断有机生产中肥料施用... 有机农业在世界范围内正呈现快速发展的趋势,有机产品识别受到人们的普遍关注。目前已有研究者提出将产品^(15)N自然丰度(^(δ)15 N值)作为其鉴别指标。介绍了稳定性氮同位素的相关知识,解释了^(δ)15 N识别技术判断有机生产中肥料施用类型的可行性,并指出可能引起该技术鉴别误差的主要影响因素。建议在尽可能全面分析各个影响因素的基础上,结合^(δ)15 N识别技术与其他的认证管理措施,提高有机农产品鉴别的准确度。 展开更多
关键词 ^^(δ)15 n 有机产品鉴别 稳定性同位素 氮素
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Tree-ring δ^(15)N of Qinghai spruce in the central Qilian Mountains of China:Is pre-treatment of wood samples necessary? 被引量:2
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作者 WANG Ziyi LIU Xiaohong +5 位作者 WANG Keyi ZENG Xiaomin ZHANG Yu GE Wensen KANG Huhu LU Qiangqiang 《Journal of Arid Land》 SCIE CSCD 2022年第6期673-690,共18页
A knowledge of the tree-ring stable nitrogen isotope ratio(δ^(15)N)can deepen our understanding of forest ecosystem dynamics by indicating the long-term availability,cycling and sources of nitrogen(N).However,the rad... A knowledge of the tree-ring stable nitrogen isotope ratio(δ^(15)N)can deepen our understanding of forest ecosystem dynamics by indicating the long-term availability,cycling and sources of nitrogen(N).However,the radial mobility of N blurs the interannual variations in the long-term N records.Previous studies of the chemical extraction of tree rings before analysis had produced inconsistent results and it is still unclear whether it is necessary to pre-treat wood samples from specific tree species to remove soluble N compounds before determining theδ^(15)N values.We compared the effects of pre-treatment with organic solvents and hot ultrapure water on the N concentration andδ^(15)N of tree rings from endemic Qinghai spruce(Picea crassifolia)growing in the interior of the central Qilian Mountains,China,during the last 60 a.We assessed the effects of different preparation protocols on the removal of the labile N compounds and investigated the need to pre-treat wood samples before determining theδ^(15)N values of tree rings.Increasing trends of the tree-ring N concentration were consistently observed in both the extracted and unextracted wood samples.The total N removed by extraction with organic solvents was about 17.60%,with a significantly higher amount in the sapwood section(P<0.01).Theδ^(15)N values of tree rings decreased consistently from 1960 to 2019 in both the extracted and unextracted wood samples.Extraction with organic solvents increased theδ^(15)N values markedly by about 5.2‰and reduced the variations in theδ^(15)N series.However,extraction with hot ultrapure water had little effect,with only a slight decrease in theδ^(15)N values of about 0.5‰.Our results showed that the radial pattern in the inter-ring movement of N in Qinghai spruce was not minimized by extraction with either organic solvents or hot ultrapure water.It is unnecessary to conduct hot ultrapure water extraction for the wood samples from Qinghai spruce because of its negligible effect on the removal of the labile N.Theδ^(15)N variation trend of tree rings in the unextracted wood samples was not influenced by the heartwood-sapwood transition zone.We suggest that theδ^(15)N values of the unextracted wood samples of the climate-sensitive Qinghai spruce could be used to explore the ecophysiological dynamics while focusing on the long-term variations. 展开更多
关键词 tree rings stable nitrogen isotope ^ratio(δ^(15)n) nitrogen concentration solvent-extracted wood water-extracted wood wood pre-treatment Qinghai spruce Qilian Mountains
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施肥对菜地土壤、蔬菜、径流中δ^(15)N组成的影响
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作者 张丽君 胡燕芳 +1 位作者 王京文 章明奎 《农学学报》 2021年第7期28-31,共4页
为了解施肥对蔬菜地土壤、蔬菜和地表水体中δ^(15)N丰度的影响,选择2012至2018内施用不同肥料的28块蔬菜地(包括13块施用化肥蔬菜地、8块施用畜禽粪蔬菜地和7块施用商品有机肥加化肥蔬菜地),采样分析表层土壤、蔬菜地上部分和地下部分... 为了解施肥对蔬菜地土壤、蔬菜和地表水体中δ^(15)N丰度的影响,选择2012至2018内施用不同肥料的28块蔬菜地(包括13块施用化肥蔬菜地、8块施用畜禽粪蔬菜地和7块施用商品有机肥加化肥蔬菜地),采样分析表层土壤、蔬菜地上部分和地下部分及地表径流样的δ^(15)N丰度。结果表明:施肥种类对土壤、蔬菜和地表径流中δ^(15)N丰度均可产生显著影响,其丰度值由高至低依次为:畜禽肥>商品有机肥加化肥>化肥。蔬菜和地表水与土壤之间的δ^(15)N丰度存在正相关。分析认为,蔬菜产品中氮同位素组成可以作为鉴定有机蔬菜的辅助依据,地表水体中δ^(15)N丰度可作为水体中氮素污染源的指标。 展开更多
关键词 蔬菜地 ^δ^(15)n丰度 有机蔬菜 有机肥 化肥 污染源识别
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Trace element geochemistry and stable isotopic(δ^(13)C andδ^(15)N)records of the Paleocene coals,Salt Range,Punjab,Pakistan 被引量:1
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作者 Noshin Masood Tehseen Zafar +2 位作者 Karen A.Hudson-Edwards Hafiz U.Rehman Abida Farooqi 《International Journal of Mining Science and Technology》 SCIE EI CAS CSCD 2022年第3期551-561,共11页
The Paleocene coals of the Salt Range in the Punjab Province of Pakistan have great economic potential;however,their trace element and stable isotopic characteristics have not been studied in detail except for a few s... The Paleocene coals of the Salt Range in the Punjab Province of Pakistan have great economic potential;however,their trace element and stable isotopic characteristics have not been studied in detail except for a few sporadic samples.In this study,a total of 59 coal samples of which 14 are obtained from open cast mines have been investigated for elemental composition andδ^(13)C-δ^(15)N isotopic signatures.Average contents of trace elements such as Co,Cr,Cu,Pb,Sr,Th,U,V,and Zn are 7.4,41.7,11.2,12.5,90.2,4.0,1.9,128,and 31.1 mg/kg,respectively.These values,when compared with the World Coal Clarke values,were relatively higher in low-rank coals in comparison with Clarke values for brown coals.Likewise,As(20.4 mg/kg),Co(6.6 mg/kg),Cr(22.4 mg/kg),Cu(^(13).3 mg/kg),Pb(19.2 mg/kg),Sr(^(15)4.7 mg/kg),Th(2.5 mg/kg),V(47.8 mg/kg),and Zn(75.1 mg/kg)were significantly higher in the sub-bituminous to bituminous coals of the Salt Range.Mineralogical analysis,based on X-ray diffraction and energy dispersive X-ray spectroscopy,revealed that the studied samples contain illite,kaolinite calcite,gypsum,pyrite,and quartz.Elemental affinity with organic and inorganic phases of coals calculated by an indirect statistical approach indicated a positive association of ash content with Ag,Al,Co,Cr,Cs,Cu,Mn,P,Rb,Pb,Th,U,and V,suggesting the presence of inorganic components in studied coals.However,As,Fe,Sr,and Zn exhibit negative correlations that imply their association with the organic fraction.Theδ^(13)C andδ^(15)N isotopic range and average−24.94‰to−25.86‰(−25.41‰)and−2.77‰to 3.22‰(0.96‰),respectively,reflecting 3C type modern terrestrial vegetation were common in the palaeomires of studied coal seams.In addition,the trivial variations of 0.92‰and 0.45‰among^(13)C and^(15)N values can be attributed to water level fluctuations and plant assemblies. 展开更多
关键词 Coal Salt Range Pakistan Geochemistry Trace elements ^δ^(13)C ^andδ^(15)n isotopes
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Determination of trophic levels of marine fish in the Yellow Sea and northern East China Sea using nitrogen stable isotope (δ^(15)N) analysis of otoliths 被引量:1
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作者 Huaiyu BAI Yukun WANG +3 位作者 Tingting ZHANG Fangqun DAI Lingfeng HUANG Yao SUN 《Journal of Oceanology and Limnology》 SCIE CAS CSCD 2022年第2期634-642,共9页
Fish otolithδ^(15) N(δ^(15) N_(oto))is a demonstrated source of information of dietary history for marine fi sh as it is available iN_(oto)lith archives and sedimentary deposits unlike white muscle tissue(WMT).WMT a... Fish otolithδ^(15) N(δ^(15) N_(oto))is a demonstrated source of information of dietary history for marine fi sh as it is available iN_(oto)lith archives and sedimentary deposits unlike white muscle tissue(WMT).WMT and stomach content data are insufficient for trophic level(TL)data of past fi shes which is important for the changes of marine fi shery resources over long time scales.To determine the correlation betweenδ^(15) N_(oto) and fi sh WMTδ^(15) N(δ^(15) N_(wmt))and the feasibility of usingδ^(15) N_(oto) in characterizing the TLs of marine fi shes,we conducted nitrogen stable isotope analysis(SIA)in the otolith and WMT of 36 marine fi sh species sampled from the Yellow Sea and northern East China Sea in 2011-2014.Bothδ^(15) N_(oto) andδ^(15) N_(wmt) were analyzed using an elemental analyzer coupled with an isotope ratio mass spectrometer(EA-IRMS).Multiple otoliths were combined to make each otolith measurement and were analyzed as-is without a carbonate dissolution pre-processing step.δ^(15) N_(oto) andδ^(15) N_(wmt) comparisons for species in the Yellow Sea and northern East China Sea are currently lacking and would be helpful for both regional studies and for increasing the number of species for whichδ^(15) N_(oto) andδ^(15) N_(wmt) have been compared.Additionally,to determine the relative accuracy of trophic level calculated usingδ^(15) N_(oto),we compared TL calculated fromδ^(15) N_(oto) to traditional trophic level metrics calculated usingδ^(15) N_(wmt).The results showed a positive and highly signifi cant correlation(R=0.780,P<0.001)betweenδ^(15) N_(oto) andδ^(15) N_(wmt).Trophic level estimation using WMT(TL wmt)and otolith(TL oto)showed congruence in our study,which is not entirely surprising given thatδ^(15) N_(oto) was regressed againstδ^(15) N_(wmt) and the resulting regression coefficient was used to convertδ^(15) N_(oto) toδ^(15) N_(wmt) prior to calculating TL oto.This conversion was required in order to be consistent with previousδ^(15) N_(wmt)-based calculations of TL for comparison.TL oto calculations resulted in TL values that were largely within 5%-10%of TL values calculated withδ^(15) N_(wmt).Our fi ndings show thatδ^(15) N_(oto) is a feasible technique for characterizing the TLs of marine fi sh and can also assist in food web and marine ecosystem studies. 展开更多
关键词 stable isotope analysis ^δ^(15)n OTOLITH trophic level
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The Signatures of Stable Isotopes δ^(15)N and δ^(13)C in Anadromous and Non-Anadromous Coilia nasus Living in the Yangtze River, and the Adjacent Sea Waters 被引量:1
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作者 WANG Lei TANG Wenqiao DONG Wenxia 《Journal of Ocean University of China》 SCIE CAS 2015年第6期1053-1058,共6页
Stable isotopes are increasingly used to investigate seasonal migrations of aquatic organisms. This study employed stable isotopes (delta C-13 and delta N-15) for Coilia nasus from the lower Yangtze River and the adja... Stable isotopes are increasingly used to investigate seasonal migrations of aquatic organisms. This study employed stable isotopes (delta C-13 and delta N-15) for Coilia nasus from the lower Yangtze River and the adjacent East China Sea to distinguish different ecotypic groups, ascertain trophic nutrition positions, and reflect environmental influences on C. nasus. delta C-13 signatures of C. nasus sampled from Zhoushan (ZS), Chongming (CM), and Jingjiang (JJ) waters were significantly higher than those from the Poyang Lake (PYL) (P < 0.05). By contrast, delta N-15 signatures of C. nasus in ZS, CM, and JJ groups were significantly lower than those in PYL group (P < 0.05). Basing on delta C-13 and delta N-15 signatures, we could distinguish anadromous (ZS, CM, and JJ) and non-anadromous (PYL) groups. The trophic level (TL) of anadromous C. nasus ranged from 2.90 to 3.04, whereas that of non-anadromous C. nasus was 4.38. C. nasus occupied the middle and top nutrition positions in the marine and Poyang Lake food webs, respectively. C. nasus in Poyang Lake were significantly more enriched in delta N-15 but depleted in delta C-13, suggesting that anthropogenic nutrient inputs and terrigenous organic carbon are important to the Poyang Lake food web. This study is the first to apply delta N-15 and delta C-13 to population assignment studies of C. nasus in the Yangtze River and its affiliated waters. Analysis of stable isotopes (delta N-15 and delta C-13) is shown to be a useful tool for discriminating anadromous and non-anadromous C. nasus. 展开更多
关键词 Japanese grenadier anchovy delta C-13 delta n-15 Changjiang River Poyang Lake
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Variations in the natural 13C and 15N abundance of plants and soils under long-term N addition and precipitation reduction:interpretation of C and N dynamics
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作者 Guoyong Yan Shijie Han +5 位作者 Mingxin Zhou Wenjing Sun Binbin Huang Honglin Wang Yajuan Xing Qinggui Wang 《Forest Ecosystems》 SCIE CSCD 2020年第4期646-658,共13页
Background:The nitrogen isotope natural abundance(δ^(15)N)provides integrated information on ecosystem N dynamics,and carbon isotope natural abundance(δ^(13)C)has been used to infer how water-using processes of plan... Background:The nitrogen isotope natural abundance(δ^(15)N)provides integrated information on ecosystem N dynamics,and carbon isotope natural abundance(δ^(13)C)has been used to infer how water-using processes of plants change in terrestrial ecosystems.However,howδ^(13)C andδ^(15)N abundances in plant life and soils respond to N addition and water availability change is still unclear.Thus,δ^(13)C andδ^(15)N abundances in plant life and soils were used to investigate the effects of long-time(10 years)N addition(+50 kg N·ha^(−1)·yr^(−1)and precipitation reduction(−30%of throughfall)in forest C and N cycling traits in a temperate forest in northern China.Results:We analyzed theδ^(13)C andδ^(15)N values of dominant plant foliage,litterfall,fungal sporophores,roots,and soils in the study.The results showed thatδ^(15)N values of foliage,litterfall,and surface soil layer’s(0–10 cm)total N were significantly increased by N addition,whileδ^(15)N values of fine roots and coarse roots were considerably decreased.Nitrogen addition also significantly increased theδ^(13)C value of fine roots and total N concentration of the surface soil layer compared with the control.The C concentration,δ^(13)C,andδ^(15)N values of foliage andδ^(15)N values of fine roots were significantly increased by precipitation reduction,while N concentration of foliage and litterfall significantly decreased.The combined effects of N addition and precipitation reduction significantly increased theδ^(13)C andδ^(15)N values of foliage as well as theδ^(15)N values of fine roots andδ^(13)C values of litterfall.Furthermore,foliarδ^(15)N values were significantly correlated with foliageδ^(13)C values,surface soilδ^(15)N values,surface soil C concentration,and N concentrations.Nitrogen concentrations andδ^(13)C values of foliage were significantly correlated withδ^(15)N values and N concentrations of fine roots.Conclusions:This indicates that plants increasingly take up the heavier 15N under N addition and the heavier 13C and 15N under precipitation reduction,suggesting that N addition and precipitation reduction may lead to more open forest ecosystem C and N cycling and affect plant nutrient acquisition strategies. 展开更多
关键词 ^δ^(13)C ^δ^(15)n n addition Precipitation reduction nutrient acquisition strategies
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Sedimentary record of climate change in a high latitude fjord—Kongsfjord
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作者 Hang Wu Binbin Deng +5 位作者 Jinlong Wang Sheng Zeng Juan Du Peng Yu Qianqian Bi Jinzhou Du 《Acta Oceanologica Sinica》 SCIE CAS CSCD 2023年第1期91-102,共12页
The sedimentary record of climate change in the Arctic region is useful for understanding global warming.Kongsfjord is located in the subpolar region of the Arctic and is a suitable site for studying climate change.Gl... The sedimentary record of climate change in the Arctic region is useful for understanding global warming.Kongsfjord is located in the subpolar region of the Arctic and is a suitable site for studying climate change.Glacier retreat is occurring in this region due to climate change,leading to an increase in meltwater outflow with a high debris content.In August 2017,we collected a sediment Core Z3 from the central fjord near the Yellow River Station.Then,we used the widely used chronology method of 210Pb,^(137)Cs,and other parameters to reflect the climate change record in the sedimentary environment of Kongsfjord.The results showed that after the mid-late 1990s,the mass accumulation rate of this core increased from 0.10 g/(cm^(2)·a)to 0.34 g/(cm^(2)·a),while the flux of^(210)Pb_(ex)increased from 125 Bq/(m^(2)·a)to 316 Bq/(m^(2)·a).The higher sedimentary inventory of^(210)Pb_(ex)in Kongsfjord compared to global fallout might have been caused by sediment focusing,boundary scavenging,and riverine input.Similarities between the inventory of^(137)Cs and global fallout indicated that terrestrial particulate matter was the main source of^(137)Cs in fjord sediments.The sedimentation rate increased after 1997,possibly due to the increased influx of glacial meltwater containing debris.In addition,the^(137)Cs activity,percentage of organic carbon(OC),and OC/total nitrogen concentration ratio showed increasing trends toward the top of the core since 1997,corresponding to a decrease in the mass balance of glaciers in the region.The results ofδ^(13)C,δ^(15)N and OC/TN concentration ratio showed both terrestrial and marine sources contributed to the organic matter in Core Z3.The relative contribution of terrestrial organic matter which was calculated by a two-endmember model showed an increased trend since mid-1990s.All these data indicate that global climate change has a significant impact on Arctic glaciers. 展开更多
关键词 Kongsfjord RADIOnUCLIDE organic carbon/total nitrogen(OC/Tn)concentration ratio ^δ^(13)C ^δ^(15)n sediment record climate change
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基于特征脂肪酸和稳定同位素技术的新疆长身高原鳅摄食习性研究
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作者 张丽蓉 郝慧敏 +2 位作者 聂竹兰 魏杰 张蝶燕 《南方水产科学》 CAS CSCD 北大核心 2024年第2期92-101,共10页
长身高原鳅(Triplophysa tenuis)对维持木扎提河的水生态系统物质和能量流动具有重要作用。文章基于脂肪酸生物标记法和碳、氮稳定同位素(δ^(13)C、δ^(15)N)技术研究了长身高原鳅的食性和营养生态位特征。结果显示,其肌肉中共检测出2... 长身高原鳅(Triplophysa tenuis)对维持木扎提河的水生态系统物质和能量流动具有重要作用。文章基于脂肪酸生物标记法和碳、氮稳定同位素(δ^(13)C、δ^(15)N)技术研究了长身高原鳅的食性和营养生态位特征。结果显示,其肌肉中共检测出22种脂肪酸,其中有8种饱和脂肪酸、6种单不饱和脂肪酸、8种多不饱和脂肪酸;由特征脂肪酸组成情况推测,长身高原鳅对浮游动物、硅藻、陆地植物或喜摄食硅藻的鱼虾均有摄食,表现为杂食性。长身高原鳅的δ^(13)C和δ^(15)N分别介于-27.09‰~-20.98‰和5.71‰~8.45‰,营养级介于2.68~3.48。雌雄样本间的δ^(13)C、δ^(15)N和营养级均不存在显著性差异(P>0.05);雄性样本核心生态位(Standard ellipse area,SEAc)和总生态位(Total area of convex hull,TA)均高于雌性。δ^(13)C与体长间表现为极显著正相关性(P<0.01),δ^(15)N和营养级与体长间不具有显著相关性。70~80 mm体长组与90 mm以上体长组SEAc面积不存在重叠且在聚类分析中被分为不同组。综上,长身高原鳅的食性为杂食性且食物组成随体长变化而不同,作为营养级偏高的捕食者,长身高原鳅能延长食物链长度,增加食物网复杂性,有利于维持水域生态系统的稳定性。 展开更多
关键词 长身高原鳅 脂肪酸 ^δ^(13)C ^δ^(15)n 食性 生态位 营养级
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基于同位素特征的华北平原菜地N_2O排放监测中取样时间的探讨 被引量:2
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作者 房福力 李玉中 《植物营养与肥料学报》 CAS CSCD 北大核心 2016年第4期978-987,共10页
【目的】为了长期监测土壤释放N_2O的通量和同位素变化规律,了解产生N_2O的微生物过程,提高对N_2O排放量和排放系数估计的准确性,需要对N_2O的日变化规律做深入研究,以便获得具有代表性的取样时间点及密闭时间。【方法】采用田间原位试... 【目的】为了长期监测土壤释放N_2O的通量和同位素变化规律,了解产生N_2O的微生物过程,提高对N_2O排放量和排放系数估计的准确性,需要对N_2O的日变化规律做深入研究,以便获得具有代表性的取样时间点及密闭时间。【方法】采用田间原位试验对华北平原的莴苣菜地进行了N_2O排放监测,选取N_2O排放高峰期即施肥灌溉后5 6 d为监测时间段,采用静态气体箱收集土壤释放的N_2O气体,结合气相色谱和质谱技术测定N_2O的含量及其同位素值(δ^(15)N-N_2O,δ^(18)O-N_2O和SP)。试验设2种取样间隔,即2 h和10 min,分别对N_2O日变化规律和密闭时间进行研究。【结果】1)在莴苣菜地N_2O排放高峰期内,N_2O通量日变化范围为34.65 131.45μg/(m^2·h),最大和最小的通量分别发生在13:00和次日5:00,9:00的N_2O通量为83.66μg/(m^2·h),与日通量平均值82.81μg/(m^2·h)相接近。N_2O通量产生日变化的原因与土壤温度有关,相关分析表明,N_2O通量与地下5 cm处土壤温度呈显著正相关(R2=0.82,P<0.01),而与土壤充水孔隙度(WFPS)无显著相关性。2)24 h内,δ^(15)Nbulk-N_2O和δ^(18)O-N_2O随着时间呈现先降低后增加的变化趋势,变化范围分别为-31.22‰-11.09‰和-7.45‰-0.68‰;SP值随时间呈现先增加后降低的变化趋势,变化范围为16.13‰26.41‰。N_2O各个同位素值随时间的变化表明产生N_2O的微生物过程随之变化,但SP值在9:00 17:00较稳定,变化范围为23.26‰26.21‰,极显著高于其他时刻(P<0.01),表明硝化作用在这一时间段内对N_2O的产生起主导作用。3)扣箱40min后,N_2O含量、δ15N-N_2O和SP值都达到稳定状态,因此选取40 min作为单次观测N_2O含量和同位素变化的密闭时间。4)24 h内N_2O通量加权SP值为22.54‰。根据前人总结的规律,本研究中N_2O主要由细菌硝化作用产生,且估计60.92%的N_2O来自于细菌硝化作用,39.08%的N_2O来自于反硝化作用。【结论】华北平原莴苣菜地的N_2O通量和同位素值具有较大的日变化,综合N_2O通量和同位素值,建议选取9:00作为观测莴苣菜地N_2O排放通量和同位素特征值变化规律的时刻,建议静态气体箱密闭时间为40 min。 展开更多
关键词 n_2O通量 δ-(15)n-n_2O δ-(18)O-n_2O SP 日变化
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四川盆地农业区河流型水库流域地下水硝酸盐来源解析及健康风险研究
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作者 何可馨 徐芬 +5 位作者 周亚倩 覃杰 邬丽姗 何小霞 汪宏 杨睿 《湖泊科学》 EI CAS CSCD 北大核心 2024年第4期1144-1151,I0019,I0020,共10页
地下水硝酸盐(NO_(3)^(-))污染是一个全球性的环境问题,尤其在农业区普遍存在。地下水中NO_(3)^(-)可以通过交互作用进入地表水中,是地表水NO_(3)^(-)的潜在来源。研究地下水中NO_(3)^(-)的来源及其贡献率对防治周边地表水NO_(3)^(-)污... 地下水硝酸盐(NO_(3)^(-))污染是一个全球性的环境问题,尤其在农业区普遍存在。地下水中NO_(3)^(-)可以通过交互作用进入地表水中,是地表水NO_(3)^(-)的潜在来源。研究地下水中NO_(3)^(-)的来源及其贡献率对防治周边地表水NO_(3)^(-)污染具有重要意义。本研究以四川盆地农业区河流型水库周边地下水为研究对象,综合运用水文地球化学、多种稳定同位素(δD-H_(2)O和δ^(18) O-H_(2)O,δ^(15) N-NO_(3)^(-)和δ^(18)O-NO_(3)^(-))、贝叶斯同位素混合模型(SIAR)和人类健康风险评价(HHRA)解析地下水中NO_(3)^(-)的来源与转化过程、不同NO_(3)^(-)来源的贡献率及潜在的人类健康风险。结果表明:丰、枯水期地下水中NO_(3)^(-)-N浓度分别为1.24~42.91和0~42.96 mg/L,61%和40%的地下水样品超过了饮用水限值(10 mg/L)。δ^(15) N-NO_(3)^(-)和δ^(18)O-NO_(3)^(-)表明,研究区地下水中NO_(3)^(-)的主要来源为粪肥/生活污水。硝化作用可能是研究区地下水中重要的氮循环过程,存在加剧地下水NO_(3)^(-)污染的风险。SIAR模型得出丰、枯水期地下水中NO_(3)^(-)的主要来源为粪肥/生活污水,贡献率分别为50%和38%。HHRA表明长期饮用研究区NO_(3)^(-)浓度较高的地下水对人类健康具有潜在风险。 展开更多
关键词 四川盆地 河流型水库 地下水 硝酸盐 氮氧同位素 贝叶斯同位素混合模型(SIAR) 健康风险评估
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紫花苜蓿叶片和根系膜脂过氧化及C、N特征对水分和N添加的响应 被引量:2
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作者 白婕 臧真凤 +5 位作者 刘丛 昝看卓 龙明秀 王可珍 屈洋 何树斌 《草业学报》 CSCD 北大核心 2022年第2期213-220,共8页
为研究紫花苜蓿叶片和根系对水分和外源氮(N)添加的响应规律,在温室条件下设置水分胁迫处理(WS)(35%±5%)田间持水量(field water capacity,FWC)和充分灌溉且未渍水(WW)(70%±5%)FWC两个水分梯度,每个水分梯度下设置0、5和10 mm... 为研究紫花苜蓿叶片和根系对水分和外源氮(N)添加的响应规律,在温室条件下设置水分胁迫处理(WS)(35%±5%)田间持水量(field water capacity,FWC)和充分灌溉且未渍水(WW)(70%±5%)FWC两个水分梯度,每个水分梯度下设置0、5和10 mmol·L^(-1)3个N添加水平(Nn、Nm和Nh),研究了紫花苜蓿叶片和根系膜脂过氧化的程度及C、N特征对不同水分条件和外源N添加的响应规律。结果表明:WS和外源N提高了紫花苜蓿叶片丙二醛(MDA)含量,但对根系没有显著影响。WS和N添加未影响紫花苜蓿叶片C含量,但N添加提高了根系C含量。WS未改变紫花苜蓿叶片N含量,但提高了根系N含量。外源N添加不但提高了叶片N含量,还增加了根系N含量,但叶片N含量在WW处理下对外源N添加较为敏感,而根系N含量在WS处理下对外源N的添加较为敏感,这说明紫花苜蓿叶片和根系C、N状态对N添加的响应受土壤水分条件的调控。紫花苜蓿根系C/N较叶片更高,且对水分和外源N添加的响应更为敏感。WS处理显著提高了根系δ^(13)C,对叶片δ^(13)C无显著影响。外源N添加降低了叶片和根系δ^(15)N,且在WS处理下根系δ^(15)N显著降低,叶片中δ^(15)N在WW处理下显著降低。总之,相比叶片,紫花苜蓿根系生理参数及C、N特征对水分和外源N添加采取了更为积极的策略,在生长中发挥着更重要的作用。该研究结果有助于全面掌握紫花苜蓿各器官对水分和外源N添加的响应策略,为我国旱作农业区紫花苜蓿制定精准的水肥管理制度提供了理论依据。 展开更多
关键词 紫花苜蓿 叶片和根系互作 ^δ^(13)C ^δ^(15)n 水分胁迫
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Responses of nutrient biogeochemistry and nitrogen cycle to seasonal upwelling in coastal waters of the eastern Hainan Island 被引量:1
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作者 Nan Zhou Sumei Liu +3 位作者 Guodong Song Yunyan Zhang Lingyan Wang Xiaoyan Ning 《Acta Oceanologica Sinica》 SCIE CAS CSCD 2022年第6期99-113,共15页
The coastal upwelling has profound influence on the surrounding ecosystem by supplying the nutrient-replete water to the euphotic zone.Nutrient biogeochemistry was investigated in coastal waters of the eastern Hainan ... The coastal upwelling has profound influence on the surrounding ecosystem by supplying the nutrient-replete water to the euphotic zone.Nutrient biogeochemistry was investigated in coastal waters of the eastern Hainan Island in summer 2015 and autumn 2016.From perspectives of nutrient dynamics and physical transport,the nutrient fluxes entered the upper 50 m water depth(between the mixed layer and the euphotic zone)arisen from the upwelling were estimated to be 2.5-5.4 mmol/(m^(2)·d),0.15-0.28 mmol/(m^(2)·d),and 2.2-7.2 mmol/(m^(2)·d)for dissolved inorganic nitrogen(DIN),phosphate(DIP),and dissolved silicate(DSi),respectively,which were around 6-to 12-fold those in the background area.The upwelled nutrients supported an additional plankton growth of(14.70±8.95)mg/m^(2)for chlorophyll a(Chl a).The distributions of nitrateδ^(15)N andδ^(18)O above the 300 m water depth(top of the North Pacific Intermediate Water)were different among the upwelling area,background area in summer,and the stations in autumn,and the difference of environmental and biogeochemical conditions between seasons should be the reason.The higher DIN/DIP concentration ratio,nitrate concentration anomaly,and lower nitrate isotope anomaly(Δ(15,18))in the upper ocean in summer than in autumn indicated the stronger nitrogen fixation and atmospheric deposition,and the following fixed nitrogen regeneration in summer.The higher values of Chl a and nitrateδ^(15)N andδ^(18)O within the euphotic zone in autumn than the background area in summer suggested the stronger nitrate assimilation in autumn.The differences in relatively strength of the assimilation,nitrogen fixation and atmospheric deposition,and the following remineralization and nitrification between the two seasons made the higherδ^(18)O:δ^(15)N and larger difference of enzymatic isotope fractionation factors^(15)εand^(18)εfor nitrate assimilation in summer than in autumn above the North Pacific Tropical Water. 展开更多
关键词 nUTRIEnTS UPWELLInG ^nitrateδ^(15)n ^andδ^(18)O nitrogen cycle South China Sea
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基于氮氧同位素的南四湖硝酸盐来源解析 被引量:8
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作者 凌郡鸿 张依章 +2 位作者 曹英杰 李旭 赵光磊 《中国环境科学》 EI CAS CSCD 北大核心 2023年第6期3100-3106,共7页
选取南水北调东线受水湖南四湖为研究对象,运用δ^(15)N-NO_(3)^(-)、δ^(18)O-NO_(3)^(-)同位素示踪技术和水化学分析方法,阐明了研究区水化学及不同形态氮分布特征,揭示了氮的转化过程,分析了硝酸盐来源,基于MixSIAR模型,对研究区水... 选取南水北调东线受水湖南四湖为研究对象,运用δ^(15)N-NO_(3)^(-)、δ^(18)O-NO_(3)^(-)同位素示踪技术和水化学分析方法,阐明了研究区水化学及不同形态氮分布特征,揭示了氮的转化过程,分析了硝酸盐来源,基于MixSIAR模型,对研究区水体中各硝酸盐来源贡献比例进行了定量识别.结果表明:南四湖无明显温跃层,水体呈碱性,水化学类型以SO_(4)^(2-)-Na^(+)型为主.下级湖中的氮以硝态氮为主,随着水体自净及沉积物吸附,浓度逐渐降低,入湖河流污染特征与湖水一致.研究区湖水硝酸盐形成过程以硝化作用为主,水体中的硝酸盐来源生活污水>土壤有机氮>合成化肥>大气沉降,基于MixSIAR源解析模型分析,贡献比例分别为51.3%、23.7%、16.4%、8.5%. 展开更多
关键词 氮氧同位素 硝酸盐 源解析 南四湖 MixSIAR
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