Three materials(agar,konjac glucomannan(KGM)andκ-carrageenan)were used to prepare ternary systems,i.e.,sol-gels and their dried composites conditioned at varied relative humidity(RH)(33%,54%and 75%).Combined methods,...Three materials(agar,konjac glucomannan(KGM)andκ-carrageenan)were used to prepare ternary systems,i.e.,sol-gels and their dried composites conditioned at varied relative humidity(RH)(33%,54%and 75%).Combined methods,e.g.,scanning electron microscopy,small-angle X-ray scattering,infrared spectroscopy(IR)and X-ray diffraction(XRD),were used to disclose howκ-carrageenan addition tailors the features of agar/KGM/κ-carrageenan ternary system.As affirmed by IR and XRD,the ternary systems withκ-carrageenan below 25%(agar/KGM/carrageenan,50:25:25,m/m)displayed proper component interactions,which increased the sol-gel transition temperature and the hardness of obtained gels.For instance,the ternary composites could show hardness about 3 to 4 times higher than that for binary counterpart.These gels were dehydrated to acquire ternary composites.Compared to agar/KGM composite,the ternary composites showed fewer crystallites and nanoscale orders,and newly-formed nanoscale structures from chain assembly.Such multi-scale structures,for composites withκ-carrageenan below 25%,showed weaker changes with RH,as revealed by especially morphologic and crystalline features.Consequently,the ternary composites with lessκ-carrageenan(below 25%)exhibited stabilized elongation at break and hydrophilicity at different RHs.This hints to us that agar/KGM/κ-carrageenan composite systems can display series applications with improved features,e.g.,increased sol-gel transition point.展开更多
Carrageenans are widely utilized in many commercial applications such as the food and pharmaceutical industry, due to their excellent functional properties. In this study, a sensitive LC-MS/MS method was developed to ...Carrageenans are widely utilized in many commercial applications such as the food and pharmaceutical industry, due to their excellent functional properties. In this study, a sensitive LC-MS/MS method was developed to determine κ-3, κ-5, and κ-7 carrageenan oligosaccharides simultaneously. Optimum MRM transitions for κ-3, κ-5, and κ-7 carrageenan oligosaccharides were(645.079→565.111, [M-H]-),(515.137→474.946, [M-2H]^2-), and(471.484→445, [M-3H]^3-), respectively. Chromatographic separation was performed on an Amide column coupled with a guard column operated at 60℃ under stepwise gradient elution. The linearity of the LC-MS/MS method for κ-3, κ-5, and κ-7 carrageenan oligosaccharides, evaluated over the concentration range of 0.10- 20.0 μmol L^-1, was excellent. The precisions of the method for κ-3, κ-5, and κ-7 carrageenan oligosaccharides were from 0.91% to 9.66%, and the inter-day precisions were from 0.92% to 10.5%. Validation of the LC-MS/MS method indicated that the method was precise and in line with the CFDA guidance. This method has been successfully applied to an in vitro absorption study.展开更多
The demand for plant protein is increasing significantly due to the shortage of protein resources.Walnut protein,the main by-product of preparing walnut oil,has limited application in the food industry due to its poor...The demand for plant protein is increasing significantly due to the shortage of protein resources.Walnut protein,the main by-product of preparing walnut oil,has limited application in the food industry due to its poor solubility.It was found that the soy protein isolate(SPI)concentration had significant effects on the gel properties of the walnut protein isolate(WNPI)-κ-Carrageenan(KC)composite system treated with 15 mmol/L NaCl.The results showed that the gel strength of the composite system increased first and then decreased with the increased concentration of SPI from 0 to 2.5%.The best rheological properties,texture properties,water holding capacity((92.03±1.05)%),swelling ratio((2.04±0.19)%),freeze-thaw stability and thermal stability(85.53°C)of the composite gel were found at an SPI concentration of 1%.In the meantime,the secondary structure of protein had the least α-helix content of 10.17% and the highest β-sheet content of 39.64%,the fluorescence intensity and free sulfhydryl content reached the highest value.1% SPI could also act as a filler for WNPI to enhance the intermolecular forces such as hydrophobic interaction between the two substances,thus forming a stable gel network structure.This study can provide technical support for improving the gel properties of walnut protein and producing new plant protein gel products.展开更多
Natural biopolymers have excellent biocompatibility and biodegradability;they can be used as biomedical materials for wound healing.In this work,the Silk Fibroin(SF)andκ-carrageenan(κ-Car)composite films containing ...Natural biopolymers have excellent biocompatibility and biodegradability;they can be used as biomedical materials for wound healing.In this work,the Silk Fibroin(SF)andκ-carrageenan(κ-Car)composite films containing Zinc-doped Bioactive Glass(ZBG)have been fabricated for wound closure applications.The as-fabricated SF-κ-Car/ZBG composite films have excellent stretchability and foldability,which facilitates them for application as wound dressing.They also exhibit excellent hydrophilicity and water-absorption capacity,which can effectively absorb wound exudate and keep the wound sites under moist state.In addition,the composite films have a good antibacterial effect against S.aureus and E.coli in vitro,which can reduce the risk of wound infection.Their excellent cell compatibility is confirmed by the CCK-8 assay.The strong vascular proliferation and wound regeneration are found in SF-κ-Car/ZBG composite films on a mouse skin wound model.The SF-κCar/ZBG composite films can inhibit the secretion of inflammatory factors,and stimulate the production of vascular factors and collagen fibers.The results derived from the performed investigations revealed that the SF-κ-Car/ZBG composite films are a promising candidate dressing for wound healing applications.展开更多
为改善κ-卡拉胶在脂肪替代物制备中存在的脆性大、力学性能差和高温易分解等问题,以κ-卡拉胶和可得然胶为原料制备复合凝胶脂肪替代物(compound gel fat substitutes,CGFS),筛选出κ-卡拉胶和可得然胶最佳质量比为3∶7,考察多糖浓度...为改善κ-卡拉胶在脂肪替代物制备中存在的脆性大、力学性能差和高温易分解等问题,以κ-卡拉胶和可得然胶为原料制备复合凝胶脂肪替代物(compound gel fat substitutes,CGFS),筛选出κ-卡拉胶和可得然胶最佳质量比为3∶7,考察多糖浓度、热处理时间、热处理温度和初始pH值对κ-卡拉胶与可得然胶CGFS质构和持水能力的影响。在此基础上,以硬度为响应值优化κ-卡拉胶与可得然胶CGFS的制备工艺。结果表明:κ-卡拉胶与可得然胶CGFS的最佳制备工艺为多糖浓度3.4%、初始pH6.3、热处理时间13 min,在此条件下CGFS硬度值为(2127.432±0.480)g。展开更多
基金the National Natural Science Foundation of China(32172240)BL19U2 beamline of National Facility for Protein Science in Shanghai(NFPS)at Shanghai Synchrotron Radiation Facility,for their assistance during data collection。
文摘Three materials(agar,konjac glucomannan(KGM)andκ-carrageenan)were used to prepare ternary systems,i.e.,sol-gels and their dried composites conditioned at varied relative humidity(RH)(33%,54%and 75%).Combined methods,e.g.,scanning electron microscopy,small-angle X-ray scattering,infrared spectroscopy(IR)and X-ray diffraction(XRD),were used to disclose howκ-carrageenan addition tailors the features of agar/KGM/κ-carrageenan ternary system.As affirmed by IR and XRD,the ternary systems withκ-carrageenan below 25%(agar/KGM/carrageenan,50:25:25,m/m)displayed proper component interactions,which increased the sol-gel transition temperature and the hardness of obtained gels.For instance,the ternary composites could show hardness about 3 to 4 times higher than that for binary counterpart.These gels were dehydrated to acquire ternary composites.Compared to agar/KGM composite,the ternary composites showed fewer crystallites and nanoscale orders,and newly-formed nanoscale structures from chain assembly.Such multi-scale structures,for composites withκ-carrageenan below 25%,showed weaker changes with RH,as revealed by especially morphologic and crystalline features.Consequently,the ternary composites with lessκ-carrageenan(below 25%)exhibited stabilized elongation at break and hydrophilicity at different RHs.This hints to us that agar/KGM/κ-carrageenan composite systems can display series applications with improved features,e.g.,increased sol-gel transition point.
基金supported by the Shandong Provincial Natural Science Foundation,China (No. ZR2019BC025)Open Research Fund Program of Shandong Provincial Key Laboratory of Glycoscience&Glycotechnology (Ocean University of China)the Fundamental Research Funds for the Central Universities (Nos. 201912008,201964019)。
文摘Carrageenans are widely utilized in many commercial applications such as the food and pharmaceutical industry, due to their excellent functional properties. In this study, a sensitive LC-MS/MS method was developed to determine κ-3, κ-5, and κ-7 carrageenan oligosaccharides simultaneously. Optimum MRM transitions for κ-3, κ-5, and κ-7 carrageenan oligosaccharides were(645.079→565.111, [M-H]-),(515.137→474.946, [M-2H]^2-), and(471.484→445, [M-3H]^3-), respectively. Chromatographic separation was performed on an Amide column coupled with a guard column operated at 60℃ under stepwise gradient elution. The linearity of the LC-MS/MS method for κ-3, κ-5, and κ-7 carrageenan oligosaccharides, evaluated over the concentration range of 0.10- 20.0 μmol L^-1, was excellent. The precisions of the method for κ-3, κ-5, and κ-7 carrageenan oligosaccharides were from 0.91% to 9.66%, and the inter-day precisions were from 0.92% to 10.5%. Validation of the LC-MS/MS method indicated that the method was precise and in line with the CFDA guidance. This method has been successfully applied to an in vitro absorption study.
基金funded by the Research and Application of Key Technology of Xinjiang Walnut Oil Refining Deep Processing(2022A02004-4)the National Key R&D Program of China(2018YFD0400302)+2 种基金the Special Fund for Anhui Agriculture Research System(AHCYJSTX-NCPJG)-15the Cooperative Projects of Hefei University of Technology-Wuhan Xudong Food Co.,Ltd.(W2020JSKF0457,W2021JSKF0356)the 7^(th) Young Elite Scientist Sponsorship Program by CAST(2021QNRC001).
文摘The demand for plant protein is increasing significantly due to the shortage of protein resources.Walnut protein,the main by-product of preparing walnut oil,has limited application in the food industry due to its poor solubility.It was found that the soy protein isolate(SPI)concentration had significant effects on the gel properties of the walnut protein isolate(WNPI)-κ-Carrageenan(KC)composite system treated with 15 mmol/L NaCl.The results showed that the gel strength of the composite system increased first and then decreased with the increased concentration of SPI from 0 to 2.5%.The best rheological properties,texture properties,water holding capacity((92.03±1.05)%),swelling ratio((2.04±0.19)%),freeze-thaw stability and thermal stability(85.53°C)of the composite gel were found at an SPI concentration of 1%.In the meantime,the secondary structure of protein had the least α-helix content of 10.17% and the highest β-sheet content of 39.64%,the fluorescence intensity and free sulfhydryl content reached the highest value.1% SPI could also act as a filler for WNPI to enhance the intermolecular forces such as hydrophobic interaction between the two substances,thus forming a stable gel network structure.This study can provide technical support for improving the gel properties of walnut protein and producing new plant protein gel products.
基金supported by the Zhejiang Provincial Natural Science Foundation(LBY20H110001,LY18E030006)National Natural Science Foundation of China(51873194,51373155).
文摘Natural biopolymers have excellent biocompatibility and biodegradability;they can be used as biomedical materials for wound healing.In this work,the Silk Fibroin(SF)andκ-carrageenan(κ-Car)composite films containing Zinc-doped Bioactive Glass(ZBG)have been fabricated for wound closure applications.The as-fabricated SF-κ-Car/ZBG composite films have excellent stretchability and foldability,which facilitates them for application as wound dressing.They also exhibit excellent hydrophilicity and water-absorption capacity,which can effectively absorb wound exudate and keep the wound sites under moist state.In addition,the composite films have a good antibacterial effect against S.aureus and E.coli in vitro,which can reduce the risk of wound infection.Their excellent cell compatibility is confirmed by the CCK-8 assay.The strong vascular proliferation and wound regeneration are found in SF-κ-Car/ZBG composite films on a mouse skin wound model.The SF-κCar/ZBG composite films can inhibit the secretion of inflammatory factors,and stimulate the production of vascular factors and collagen fibers.The results derived from the performed investigations revealed that the SF-κ-Car/ZBG composite films are a promising candidate dressing for wound healing applications.
文摘为改善κ-卡拉胶在脂肪替代物制备中存在的脆性大、力学性能差和高温易分解等问题,以κ-卡拉胶和可得然胶为原料制备复合凝胶脂肪替代物(compound gel fat substitutes,CGFS),筛选出κ-卡拉胶和可得然胶最佳质量比为3∶7,考察多糖浓度、热处理时间、热处理温度和初始pH值对κ-卡拉胶与可得然胶CGFS质构和持水能力的影响。在此基础上,以硬度为响应值优化κ-卡拉胶与可得然胶CGFS的制备工艺。结果表明:κ-卡拉胶与可得然胶CGFS的最佳制备工艺为多糖浓度3.4%、初始pH6.3、热处理时间13 min,在此条件下CGFS硬度值为(2127.432±0.480)g。
