The absence of effective therapies for castration-resistant prostate cancer(CRPC) establishes the need to develop novel therapeutic modality, such as targeted gene therapy, which is ideal for the treatment of CRPC. Bu...The absence of effective therapies for castration-resistant prostate cancer(CRPC) establishes the need to develop novel therapeutic modality, such as targeted gene therapy, which is ideal for the treatment of CRPC. But its application has been limited due to lack of favorable gene vector and the reduction of "bystander effect". Consequently, scientists all over the world focus their main experimental research on the following four aspects: targeted gene, vector, transfer means and comprehensive therapy. In this paper, we reviewed the latest advances of experimental research on targeted gene therapy for prostate cancer.展开更多
Objective： To find out whether ultraviolet ray, a kind of non-ionic ray, could cause the bystander effect, the UV exposed MOLT-4 cells had been investigated. Methods： Two experiment groups were carried out, in which...Objective： To find out whether ultraviolet ray, a kind of non-ionic ray, could cause the bystander effect, the UV exposed MOLT-4 cells had been investigated. Methods： Two experiment groups were carried out, in which cells were culture and treated at two concentrations： 2 × 10^5/mL and 5 × 10^5/mL. All other treatments were the same. Part of the cells was labeled with DID and exposed to UV ray for 40 s as effect cells; other cells was untreated as bystander cells. Then, the cells were co-cultured and harvested at 4 h interval over a period of 24 h. Annexin V-FITC/PI assay was used to evaluate the bystander effect in bystander cells co-cultured with effected cells. Laser confocal microscope method was used to observe the morphologic changes of the bystander cells. Results： The percentage of cells undergoing apoptosis in the bystander cells was increased over time compared with the control group. They were 6.84%, 8.09%, 9.88%, 17.64%, 17.43%, 30.99% and 37.93% respectively in 0 h, 4 h, 8 h, 12 h, 16 h, 20 h and 24 h. When observed by laser scanning confocal microscope, the bystander cells show some classic character of apoptosis such as chromosome condense, phosphatidylsedne transfer and formation of apoptotic bodies. Conclusion： Bystander effect is significant in un-irradiated bystander MOLT-4 cells when co-cultured with UV exposed cells.展开更多
This research is based on creative industry of sports culture of the U.K. Firstly, the literature research method is used to collect relevant authoritative economic data, and clarify and identify concepts of the sport...This research is based on creative industry of sports culture of the U.K. Firstly, the literature research method is used to collect relevant authoritative economic data, and clarify and identify concepts of the sports culture creative industry with the ＂rule of thirds＂ of creative industry of the U.K.; secondly, empirical research is conducted to describe an active and drive function of the 3 kinds of expansion effects-forward effect, bystander effect and backward effect on peripheral industries on sports industry and even national economy development by rapid development of the sports culture creative industry in the U.K.; thirdly, this paper figures out that the sports culture creative industry will become new economic growth, ixew development route for the sports industry in the future and new power for industrial structure optimization and upgrading; finally, the research outcome gives reference and guide to scientific development, transformation and upgrading of the sports industry of China in the new round of worlds＇ economic restructuring.展开更多
文摘The absence of effective therapies for castration-resistant prostate cancer(CRPC) establishes the need to develop novel therapeutic modality, such as targeted gene therapy, which is ideal for the treatment of CRPC. But its application has been limited due to lack of favorable gene vector and the reduction of "bystander effect". Consequently, scientists all over the world focus their main experimental research on the following four aspects: targeted gene, vector, transfer means and comprehensive therapy. In this paper, we reviewed the latest advances of experimental research on targeted gene therapy for prostate cancer.
基金the Clinical Key Subject Foundation of HealthMinistry of China [Diagnosis and analysis of clinical tumor cell cycle (3)] and Life science Ministry Director Foundation (No.30440024)
文摘Objective： To find out whether ultraviolet ray, a kind of non-ionic ray, could cause the bystander effect, the UV exposed MOLT-4 cells had been investigated. Methods： Two experiment groups were carried out, in which cells were culture and treated at two concentrations： 2 × 10^5/mL and 5 × 10^5/mL. All other treatments were the same. Part of the cells was labeled with DID and exposed to UV ray for 40 s as effect cells; other cells was untreated as bystander cells. Then, the cells were co-cultured and harvested at 4 h interval over a period of 24 h. Annexin V-FITC/PI assay was used to evaluate the bystander effect in bystander cells co-cultured with effected cells. Laser confocal microscope method was used to observe the morphologic changes of the bystander cells. Results： The percentage of cells undergoing apoptosis in the bystander cells was increased over time compared with the control group. They were 6.84%, 8.09%, 9.88%, 17.64%, 17.43%, 30.99% and 37.93% respectively in 0 h, 4 h, 8 h, 12 h, 16 h, 20 h and 24 h. When observed by laser scanning confocal microscope, the bystander cells show some classic character of apoptosis such as chromosome condense, phosphatidylsedne transfer and formation of apoptotic bodies. Conclusion： Bystander effect is significant in un-irradiated bystander MOLT-4 cells when co-cultured with UV exposed cells.
文摘This research is based on creative industry of sports culture of the U.K. Firstly, the literature research method is used to collect relevant authoritative economic data, and clarify and identify concepts of the sports culture creative industry with the ＂rule of thirds＂ of creative industry of the U.K.; secondly, empirical research is conducted to describe an active and drive function of the 3 kinds of expansion effects-forward effect, bystander effect and backward effect on peripheral industries on sports industry and even national economy development by rapid development of the sports culture creative industry in the U.K.; thirdly, this paper figures out that the sports culture creative industry will become new economic growth, ixew development route for the sports industry in the future and new power for industrial structure optimization and upgrading; finally, the research outcome gives reference and guide to scientific development, transformation and upgrading of the sports industry of China in the new round of worlds＇ economic restructuring.
