Aim To establish an assay method for the determination of three kinds of biologically active components,five compounds（emodin,chrysophanol,baicalin,wogonin and berberine hydrochloride） simultaneously in Sanhuang tab...Aim To establish an assay method for the determination of three kinds of biologically active components,five compounds（emodin,chrysophanol,baicalin,wogonin and berberine hydrochloride） simultaneously in Sanhuang tablets.Methods HPLC was carried out,using a C18 column（150 mm×4.6 mm ID,5 μm） set at 30 ℃,acetonitrile-0.02 mol·L-1 acetic ammonium（adjusted pH to 3.50 with acetic acid glacial） as mobile phase（using gradient） with flowing rate 1.00 mL·min-1 and detected at 270 nm.Results The calibration curve of emodin was linear from（0.020 7） μg to 0.207 μg with r=0.999 9,the average recovery was 99.65% with RSD 1.25%.The calibration curve of chrysophanol was linear from 0.052 μg to 0.52 μg with r=（0.999 9）,and the average recovery was 100.36% with RSD 0.96%.The calibration curve of baicalin was linear from（0.250 5） μg to 2.505 μg with r=（0.999 8）,and the average recovery was 100.22% with RSD 1.29%.The calibration curve of wogonin was linear from 0.047 6 μg to 0.476 μg with r=（0.999 9）,and the average recovery was 98.97% with RSD 1.20%.The calibration curve of berberine hydrochloride was linear from（0.053 12） μg to 0.531 2 μg with r=（0.999 5）,and the average recovery was 96.02% with RSD 2.02%.The established method had also been used in the determination of the 5 compounds in 10 different batches of Sanhuang tablets.Conclusion This method was proved to be accurate and quick,and can be used for the quality control of the preparation all-around.展开更多
目的建立以RP-HPLC法同时分离测定三黄片中7种指标成分含量的方法。方法采用Kromasil ODS-C_(18)色谱柱(200 mm×4.6 mm,5μm),流动相A为体积分数为0.5%的三乙胺溶液-甲醇(体积比为95∶5,冰醋酸调p H 3.12)、流动相B为体积分数为0.5...目的建立以RP-HPLC法同时分离测定三黄片中7种指标成分含量的方法。方法采用Kromasil ODS-C_(18)色谱柱(200 mm×4.6 mm,5μm),流动相A为体积分数为0.5%的三乙胺溶液-甲醇(体积比为95∶5,冰醋酸调p H 3.12)、流动相B为体积分数为0.5%的三乙胺溶液-甲醇(体积比为5∶95,冰醋酸调p H 4.00)进行梯度洗脱,流速1.0 m L·min^(-1),检测波长为254 nm,柱温为25℃。结果三黄片中7种指标成分线性良好,各成分相关系数r均在0.999 8~0.999 9之间,平均回收率在97.09%~101.6%之间,RSD均低于2.0%。结论该方法测定三黄片中7种指标成分结果稳定可靠、准确,专属性强,可用于三黄片的质量控制。展开更多
文摘Aim To establish an assay method for the determination of three kinds of biologically active components,five compounds（emodin,chrysophanol,baicalin,wogonin and berberine hydrochloride） simultaneously in Sanhuang tablets.Methods HPLC was carried out,using a C18 column（150 mm×4.6 mm ID,5 μm） set at 30 ℃,acetonitrile-0.02 mol·L-1 acetic ammonium（adjusted pH to 3.50 with acetic acid glacial） as mobile phase（using gradient） with flowing rate 1.00 mL·min-1 and detected at 270 nm.Results The calibration curve of emodin was linear from（0.020 7） μg to 0.207 μg with r=0.999 9,the average recovery was 99.65% with RSD 1.25%.The calibration curve of chrysophanol was linear from 0.052 μg to 0.52 μg with r=（0.999 9）,and the average recovery was 100.36% with RSD 0.96%.The calibration curve of baicalin was linear from（0.250 5） μg to 2.505 μg with r=（0.999 8）,and the average recovery was 100.22% with RSD 1.29%.The calibration curve of wogonin was linear from 0.047 6 μg to 0.476 μg with r=（0.999 9）,and the average recovery was 98.97% with RSD 1.20%.The calibration curve of berberine hydrochloride was linear from（0.053 12） μg to 0.531 2 μg with r=（0.999 5）,and the average recovery was 96.02% with RSD 2.02%.The established method had also been used in the determination of the 5 compounds in 10 different batches of Sanhuang tablets.Conclusion This method was proved to be accurate and quick,and can be used for the quality control of the preparation all-around.
文摘目的建立以RP-HPLC法同时分离测定三黄片中7种指标成分含量的方法。方法采用Kromasil ODS-C_(18)色谱柱(200 mm×4.6 mm,5μm),流动相A为体积分数为0.5%的三乙胺溶液-甲醇(体积比为95∶5,冰醋酸调p H 3.12)、流动相B为体积分数为0.5%的三乙胺溶液-甲醇(体积比为5∶95,冰醋酸调p H 4.00)进行梯度洗脱,流速1.0 m L·min^(-1),检测波长为254 nm,柱温为25℃。结果三黄片中7种指标成分线性良好,各成分相关系数r均在0.999 8~0.999 9之间,平均回收率在97.09%~101.6%之间,RSD均低于2.0%。结论该方法测定三黄片中7种指标成分结果稳定可靠、准确,专属性强,可用于三黄片的质量控制。
